Isocitrate dehydrogenase [NADP], mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot P48735 (IDHP_HUMAN)

Last modified June 16, 2009. Version 88. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP], mitochondrial
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP
    ICD-M

Gene names

Name:

IDH2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Catalytic activity	Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH. Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

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Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 39

Mitochondrion By similarity

Chain

40 – 452

413

Isocitrate dehydrogenase [NADP], mitochondrial

PRO_0000014420

Regions

Nucleotide binding

115 – 117

NADP By similarity

Nucleotide binding

349 – 354

NADP By similarity

Region

134 – 140

Substrate binding By similarity

Sites

Metal binding

291

Magnesium or manganese By similarity

Metal binding

314

Magnesium or manganese By similarity

Binding site

117

Substrate By similarity

Binding site

122

NADP By similarity

Binding site

149

Substrate By similarity

Binding site

172

Substrate By similarity

Binding site

299

NADP By similarity

Binding site

367

NADP; via amide nitrogen and carbonyl oxygen By similarity

Site

179

Critical for catalysis By similarity

Site

251

Critical for catalysis By similarity

Amino acid modifications

Modified residue

106

N6-acetyllysine By similarity

Modified residue

272

N6-acetyllysine By similarity

Experimental info

Sequence conflict

Q → H in CAA49208. Ref.1

Sequence conflict

435

T → M in CAA49208. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P48735-1 [UniParc].

Last modified April 3, 2002. Version 2.
Checksum: 4DDC830AFC06AB52
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FASTA

452

50,909

        10         20         30         40         50         60 
MAGYLRVVRS LCRASGSRPA WAPAALTAPT SQEQPRRHYA DKRIKVAKPV VEMDGDEMTR 

        70         80         90        100        110        120 
IIWQFIKEKL ILPHVDIQLK YFDLGLPNRD QTDDQVTIDS ALATQKYSVA VKCATITPDE 

       130        140        150        160        170        180 
ARVEEFKLKK MWKSPNGTIR NILGGTVFRE PIICKNIPRL VPGWTKPITI GRHAHGDQYK 

       190        200        210        220        230        240 
ATDFVADRAG TFKMVFTPKD GSGVKEWEVY NFPAGGVGMG MYNTDESISG FAHSCFQYAI 

       250        260        270        280        290        300 
QKKWPLYMST KNTILKAYDG RFKDIFQEIF DKHYKTDFDK NKIWYEHRLI DDMVAQVLKS 

       310        320        330        340        350        360 
SGGFVWACKN YDGDVQSDIL AQGFGSLGLM TSVLVCPDGK TIEAEAAHGT VTRHYREHQK 

       370        380        390        400        410        420 
GRPTSTNPIA SIFAWTRGLE HRGKLDGNQD LIRFAQMLEK VCVETVESGA MTKDLAGCIH 

       430        440        450 
GLSNVKLNEH FLNTTDFLDT IKSNLDRALG RQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Huh T.-L., Oh I.-U., Kim Y.O., Huh J.-W., Song B.J. Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Skin.
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X69433 mRNA. Translation: CAA49208.1. BC009244 mRNA. Translation: AAH09244.1. BC071828 mRNA. Translation: AAH71828.1.
IPI	IPI00011107.
PIR	S57499.
RefSeq	NP_002159.2.
UniGene	Hs.596461
3D structure databases
HSSP	HSSP built from PDB template 1LWD based on UniProtKB P33198.
SMR	P48735. Positions 40-452.
ModBase	Search...
PTM databases
PhosphoSite	P48735.
2-D gel databases
OGP	P48735.
Proteomic databases
PeptideAtlas	P48735.
PRIDE	P48735.
Genome annotation databases
Ensembl	ENSG00000182054. Homo sapiens. [Contig view]
GeneID	3418.
KEGG	hsa:3418.
NMPDR	fig\|9606.3.peg.11234.
Organism-specific databases
GeneCards	GC15M088428.
HGNC	HGNC:5383. IDH2.
MIM	147650. gene.
PharmGKB	PA29631.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P48735.
HOVERGEN	P48735.
OMA	P48735. GGMLWAC.
Enzyme and pathway databases
BRENDA	1.1.1.42. 247.
Gene expression databases
ArrayExpress	P48735.
Bgee	P48735.
CleanEx	HS_IDH2.
GermOnline	ENSG00000182054. Homo sapiens.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	13474.
SOURCE	Search...

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Entry information

Entry name

IDHP_HUMAN

Accession

Primary (citable) accession number: P48735
Secondary accession number(s): Q96GT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	April 3, 2002
Last modified:	June 16, 2009
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

2-D gel databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents