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P48727 (GLC7B_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP1-beta

EC=3.1.3.16
Alternative name(s):
CeGLC-7-beta
Glc seven-like phosphatase 2
Gene names
Name:gsp-2
ORF Names:F56C9.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine/threonine-protein phosphatase essential for chromosomal dynamics during meiosis and mitosis. Antagonizes the function of air-2 in the regulation of chromosome cohesion. Dephosphorylates histone H3 at 'Ser-10'. Also involved in the activation of chloride channel clh-3 during cell swelling and meiotic maturation. Essential for embryogenesis. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Enzyme regulation

Inhibited by okadaic acid. Ref.5

Subcellular location

Cytoplasm. Nucleus Ref.5.

Tissue specificity

Expressed in gonads, nervous system, intestine and muscles. Ref.5

Developmental stage

Expressed at early embryonic stages. Ref.5

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Serine/threonine-protein phosphatase PP1-beta
PRO_0000058913

Sites

Active site1241Proton donor By similarity
Metal binding631Iron By similarity
Metal binding651Iron By similarity
Metal binding911Iron By similarity
Metal binding911Manganese By similarity
Metal binding1231Manganese By similarity
Metal binding1721Manganese By similarity
Metal binding2471Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
P48727 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: A73CE460AC78054E

FASTA33337,792
        10         20         30         40         50         60 
MDVEKLNLDN IISRLLEVRG SKPGKNVQLT ESEIKGLCQK SREIFLSQPI LLELEAPLKI 

        70         80         90        100        110        120 
CGDVHGQYYD LLRLFEYGGF PPESNYLFLG DYVDRGKQSL ETICLLLAYK IKYPENFFLL 

       130        140        150        160        170        180 
RGNHECASIN RIYGFYDECK RRYNIKLWKT FTDCFNCLPV AAIIDEKIFC CHGGLSPDLQ 

       190        200        210        220        230        240 
SMEQIRRIMR PTDVPDQGLL CDLLWSDPDK DVTGWGENDR GVSFTFGPEV VAKFLHKHDL 

       250        260        270        280        290        300 
DLICRAHQVV EDGYEFFAKR QLVTLFSAPN YCGEFDNAGS MMTVDETLMC SFQILKPADK 

       310        320        330 
KKYPYGAGGV GSNRPVTPPR NAPAAQPKKG AKK 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes."
Hsu J.-Y., Sun Z.-W., Li X., Reuben M., Tatchell K., Bishop D.K., Grushcow J.M., Brame C.J., Caldwell J.A., Hunt D.F., Lin R., Smith M.M., Allis C.D.
Cell 102:279-291(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis."
Rogers E., Bishop J.D., Waddle J.A., Schumacher J.M., Lin R.
J. Cell Biol. 157:219-229(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Cell cycle- and swelling-induced activation of a Caenorhabditis elegans ClC channel is mediated by CeGLC-7alpha/beta phosphatases."
Rutledge E., Denton J., Strange K.
J. Cell Biol. 158:435-444(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Role of Caenorhabditis elegans protein phosphatase type 1, CeGLC-7 beta, in metaphase to anaphase transition during embryonic development."
Sassa T., Ueda-Ohba H., Kitamura K., Harada S., Hosono R.
Exp. Cell Res. 287:350-360(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
[6]"Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis."
Sasagawa Y., Urano T., Kohara Y., Takahashi H., Higashitani A.
Genes Cells 8:857-872(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO080186 Genomic DNA. Translation: CCD61828.1.
PIRT16476.
RefSeqNP_001022616.1. NM_001027445.4.
UniGeneCel.24408.

3D structure databases

ProteinModelPortalP48727.
SMRP48727. Positions 6-298.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid532125. 1 interaction.
DIPDIP-25119N.
IntActP48727. 2 interactions.
STRING6239.F56C9.1.

Proteomic databases

PaxDbP48727.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF56C9.1.1; F56C9.1.1; F56C9.1.
F56C9.1.2; F56C9.1.2; F56C9.1.
GeneID3564807.
KEGGcel:CELE_F56C9.1.
UCSCF56C9.1. c. elegans.

Organism-specific databases

CTD3564807.
WormBaseF56C9.1; CE01319; WBGene00001748; gsp-2.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172697.
InParanoidP48727.
KOK06269.
OMAGWNENDR.
PhylomeDBP48727.

Family and domain databases

InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio954057.
PROP48727.

Entry information

Entry nameGLC7B_CAEEL
AccessionPrimary (citable) accession number: P48727
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase