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Protein

Serine/threonine-protein phosphatase PP1-beta

Gene

gsp-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein phosphatase essential for chromosomal dynamics during meiosis and mitosis. Antagonizes the function of air-2 in the regulation of chromosome cohesion. Dephosphorylates histone H3 at 'Ser-10'. Also involved in the activation of chloride channel clh-3 during cell swelling and meiotic maturation. Essential for embryogenesis.5 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited by okadaic acid.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Manganese 1By similarity1
Metal bindingi65Manganese 1By similarity1
Metal bindingi91Manganese 1By similarity1
Metal bindingi91Manganese 2By similarity1
Metal bindingi123Manganese 2By similarity1
Active sitei124Proton donorBy similarity1
Metal bindingi172Manganese 2By similarity1
Metal bindingi247Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: WormBase

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Developmental protein, Hydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Meiosis, Mitosis
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-beta (EC:3.1.3.16)
Alternative name(s):
CeGLC-7-beta
Glc seven-like phosphatase 2
Gene namesi
Name:gsp-2
ORF Names:F56C9.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF56C9.1; CE01319; WBGene00001748; gsp-2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • chromosome Source: WormBase
  • cytoplasm Source: WormBase
  • nuclear membrane Source: WormBase
  • nucleus Source: WormBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000589131 – 333Serine/threonine-protein phosphatase PP1-betaAdd BLAST333

Proteomic databases

EPDiP48727.
PaxDbiP48727.
PeptideAtlasiP48727.
PRIDEiP48727.

Expressioni

Tissue specificityi

Expressed in gonads, nervous system, intestine and muscles.1 Publication

Developmental stagei

Expressed at early embryonic stages.1 Publication

Gene expression databases

BgeeiWBGene00001748.

Interactioni

Protein-protein interaction databases

BioGridi532125. 1 interactor.
DIPiDIP-25119N.
IntActiP48727. 3 interactors.
STRINGi6239.F56C9.1.1.

Structurei

3D structure databases

ProteinModelPortaliP48727.
SMRiP48727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IN85. Eukaryota.
ENOG410XPVF. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
InParanoidiP48727.
KOiK06269.
OMAiIQRLLEX.
OrthoDBiEOG091G0EKF.
PhylomeDBiP48727.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P48727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVEKLNLDN IISRLLEVRG SKPGKNVQLT ESEIKGLCQK SREIFLSQPI
60 70 80 90 100
LLELEAPLKI CGDVHGQYYD LLRLFEYGGF PPESNYLFLG DYVDRGKQSL
110 120 130 140 150
ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRYNIKLWKT
160 170 180 190 200
FTDCFNCLPV AAIIDEKIFC CHGGLSPDLQ SMEQIRRIMR PTDVPDQGLL
210 220 230 240 250
CDLLWSDPDK DVTGWGENDR GVSFTFGPEV VAKFLHKHDL DLICRAHQVV
260 270 280 290 300
EDGYEFFAKR QLVTLFSAPN YCGEFDNAGS MMTVDETLMC SFQILKPADK
310 320 330
KKYPYGAGGV GSNRPVTPPR NAPAAQPKKG AKK
Length:333
Mass (Da):37,792
Last modified:February 1, 1996 - v1
Checksum:iA73CE460AC78054E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080186 Genomic DNA. Translation: CCD61828.1.
PIRiT16476.
RefSeqiNP_001022616.1. NM_001027445.4.
UniGeneiCel.24408.

Genome annotation databases

EnsemblMetazoaiF56C9.1; F56C9.1; WBGene00001748.
GeneIDi3564807.
KEGGicel:CELE_F56C9.1.
UCSCiF56C9.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080186 Genomic DNA. Translation: CCD61828.1.
PIRiT16476.
RefSeqiNP_001022616.1. NM_001027445.4.
UniGeneiCel.24408.

3D structure databases

ProteinModelPortaliP48727.
SMRiP48727.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi532125. 1 interactor.
DIPiDIP-25119N.
IntActiP48727. 3 interactors.
STRINGi6239.F56C9.1.1.

Proteomic databases

EPDiP48727.
PaxDbiP48727.
PeptideAtlasiP48727.
PRIDEiP48727.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF56C9.1; F56C9.1; WBGene00001748.
GeneIDi3564807.
KEGGicel:CELE_F56C9.1.
UCSCiF56C9.1. c. elegans.

Organism-specific databases

CTDi3564807.
WormBaseiF56C9.1; CE01319; WBGene00001748; gsp-2.

Phylogenomic databases

eggNOGiENOG410IN85. Eukaryota.
ENOG410XPVF. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
InParanoidiP48727.
KOiK06269.
OMAiIQRLLEX.
OrthoDBiEOG091G0EKF.
PhylomeDBiP48727.

Miscellaneous databases

PROiP48727.

Gene expression databases

BgeeiWBGene00001748.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLC7B_CAEEL
AccessioniPrimary (citable) accession number: P48727
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 15, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.