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Protein

Serine/threonine-protein phosphatase PP1-beta

Gene

gsp-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein phosphatase essential for chromosomal dynamics during meiosis and mitosis. Antagonizes the function of air-2 in the regulation of chromosome cohesion. Dephosphorylates histone H3 at 'Ser-10'. Also involved in the activation of chloride channel clh-3 during cell swelling and meiotic maturation. Essential for embryogenesis.5 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited by okadaic acid.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi63 – 631Manganese 1By similarity
Metal bindingi65 – 651Manganese 1By similarity
Metal bindingi91 – 911Manganese 1By similarity
Metal bindingi91 – 911Manganese 2By similarity
Metal bindingi123 – 1231Manganese 2By similarity
Active sitei124 – 1241Proton donorBy similarity
Metal bindingi172 – 1721Manganese 2By similarity
Metal bindingi247 – 2471Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: WormBase

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. chromatin modification Source: UniProtKB-KW
  3. meiotic cell cycle Source: UniProtKB-KW
  4. mitotic nuclear division Source: UniProtKB-KW
  5. multicellular organismal development Source: UniProtKB-KW
  6. peptidyl-serine dephosphorylation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Developmental protein, Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-beta (EC:3.1.3.16)
Alternative name(s):
CeGLC-7-beta
Glc seven-like phosphatase 2
Gene namesi
Name:gsp-2
ORF Names:F56C9.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiF56C9.1; CE01319; WBGene00001748; gsp-2.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. chromosome Source: WormBase
  2. cytoplasm Source: WormBase
  3. nuclear membrane Source: WormBase
  4. nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Serine/threonine-protein phosphatase PP1-betaPRO_0000058913Add
BLAST

Proteomic databases

PaxDbiP48727.

Expressioni

Tissue specificityi

Expressed in gonads, nervous system, intestine and muscles.1 Publication

Developmental stagei

Expressed at early embryonic stages.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi532125. 1 interaction.
DIPiDIP-25119N.
IntActiP48727. 2 interactions.
STRINGi6239.F56C9.1.

Structurei

3D structure databases

ProteinModelPortaliP48727.
SMRiP48727. Positions 6-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
InParanoidiP48727.
KOiK06269.
OMAiGWNENDR.
PhylomeDBiP48727.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVEKLNLDN IISRLLEVRG SKPGKNVQLT ESEIKGLCQK SREIFLSQPI
60 70 80 90 100
LLELEAPLKI CGDVHGQYYD LLRLFEYGGF PPESNYLFLG DYVDRGKQSL
110 120 130 140 150
ETICLLLAYK IKYPENFFLL RGNHECASIN RIYGFYDECK RRYNIKLWKT
160 170 180 190 200
FTDCFNCLPV AAIIDEKIFC CHGGLSPDLQ SMEQIRRIMR PTDVPDQGLL
210 220 230 240 250
CDLLWSDPDK DVTGWGENDR GVSFTFGPEV VAKFLHKHDL DLICRAHQVV
260 270 280 290 300
EDGYEFFAKR QLVTLFSAPN YCGEFDNAGS MMTVDETLMC SFQILKPADK
310 320 330
KKYPYGAGGV GSNRPVTPPR NAPAAQPKKG AKK
Length:333
Mass (Da):37,792
Last modified:February 1, 1996 - v1
Checksum:iA73CE460AC78054E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080186 Genomic DNA. Translation: CCD61828.1.
PIRiT16476.
RefSeqiNP_001022616.1. NM_001027445.4.
UniGeneiCel.24408.

Genome annotation databases

EnsemblMetazoaiF56C9.1; F56C9.1; WBGene00001748.
GeneIDi3564807.
KEGGicel:CELE_F56C9.1.
UCSCiF56C9.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080186 Genomic DNA. Translation: CCD61828.1.
PIRiT16476.
RefSeqiNP_001022616.1. NM_001027445.4.
UniGeneiCel.24408.

3D structure databases

ProteinModelPortaliP48727.
SMRiP48727. Positions 6-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi532125. 1 interaction.
DIPiDIP-25119N.
IntActiP48727. 2 interactions.
STRINGi6239.F56C9.1.

Proteomic databases

PaxDbiP48727.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF56C9.1; F56C9.1; WBGene00001748.
GeneIDi3564807.
KEGGicel:CELE_F56C9.1.
UCSCiF56C9.1. c. elegans.

Organism-specific databases

CTDi3564807.
WormBaseiF56C9.1; CE01319; WBGene00001748; gsp-2.

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
InParanoidiP48727.
KOiK06269.
OMAiGWNENDR.
PhylomeDBiP48727.

Miscellaneous databases

NextBioi954057.
PROiP48727.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes."
    Hsu J.-Y., Sun Z.-W., Li X., Reuben M., Tatchell K., Bishop D.K., Grushcow J.M., Brame C.J., Caldwell J.A., Hunt D.F., Lin R., Smith M.M., Allis C.D.
    Cell 102:279-291(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis."
    Rogers E., Bishop J.D., Waddle J.A., Schumacher J.M., Lin R.
    J. Cell Biol. 157:219-229(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Cell cycle- and swelling-induced activation of a Caenorhabditis elegans ClC channel is mediated by CeGLC-7alpha/beta phosphatases."
    Rutledge E., Denton J., Strange K.
    J. Cell Biol. 158:435-444(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Role of Caenorhabditis elegans protein phosphatase type 1, CeGLC-7 beta, in metaphase to anaphase transition during embryonic development."
    Sassa T., Ueda-Ohba H., Kitamura K., Harada S., Hosono R.
    Exp. Cell Res. 287:350-360(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
  6. "Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis."
    Sasagawa Y., Urano T., Kohara Y., Takahashi H., Higashitani A.
    Genes Cells 8:857-872(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGLC7B_CAEEL
AccessioniPrimary (citable) accession number: P48727
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.