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Protein

Serine/threonine-protein phosphatase PP2A catalytic subunit 1

Gene

Ppn1

Organism
Paramecium tetraurelia
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Manganese 1By similarity1
Metal bindingi64Manganese 1By similarity1
Metal bindingi90Manganese 1By similarity1
Metal bindingi90Manganese 2By similarity1
Metal bindingi122Manganese 2By similarity1
Active sitei123Proton donorBy similarity1
Metal bindingi172Manganese 2By similarity1
Metal bindingi247Manganese 2By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A catalytic subunit 1 (EC:3.1.3.16)
Short name:
PPN 1
Gene namesi
Name:Ppn1
ORF Names:GSPATT00001010001
OrganismiParamecium tetraurelia
Taxonomic identifieri5888 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaPeniculidaParameciidaeParamecium
Proteomesi
  • UP000000600 Componenti: Partially assembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588671 – 315Serine/threonine-protein phosphatase PP2A catalytic subunit 1Add BLAST315

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei315Leucine methyl esterBy similarity1

Post-translational modificationi

Reversibly methyl esterified on Leu-315 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.

Keywords - PTMi

Methylation

Interactioni

Protein-protein interaction databases

STRINGi412030.XP_001438693.1.

Structurei

3D structure databases

ProteinModelPortaliP48726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiKOG0371. Eukaryota.
COG0639. LUCA.
InParanoidiP48726.
KOiK04382.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLNKLSSN EIGNIDRQIA KLKQGQILSE QEVKSLCIKA KEILQDEPNI
60 70 80 90 100
IQVRAPLTIC GDIHGQFHDL IELFQIGGNL PDTNYLFLGD YVDRGSQSVE
110 120 130 140 150
TFSLMLSLKV RYKDRIVLLR GNHENREINK IYGFYDECFR KYGNEIVWKQ
160 170 180 190 200
FTEVFGYLPL SAIVEQQIFC AHGGLSPAME SVDQIKQLNR VQDIPHEGLM
210 220 230 240 250
CDLLWSDPEE TKNGWGISPR GAGWTWGCDI TEKFLHSNKL KQIARAHQLV
260 270 280 290 300
MEGIQKVHNQ KTITIFSAPN YCYRCGNQAC IVEVDEQLKM NQTQFEPAPR
310
ENEPHTTRRV PDYFL
Length:315
Mass (Da):36,134
Last modified:October 23, 2007 - v2
Checksum:i2C67AB335B8C4650
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34K → R in AAA68611 (Ref. 1) Curated1
Sequence conflicti47 – 49EPN → GPH in AAA68611 (Ref. 1) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27497 Genomic DNA. Translation: AAA68611.1.
CT868096 Genomic DNA. Translation: CAK71296.1.
RefSeqiXP_001438693.1. XM_001438656.1.

Genome annotation databases

EnsemblProtistsiCAK71296; CAK71296; GSPATT00001010001.
GeneIDi5024478.
KEGGiptm:GSPATT00001010001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27497 Genomic DNA. Translation: AAA68611.1.
CT868096 Genomic DNA. Translation: CAK71296.1.
RefSeqiXP_001438693.1. XM_001438656.1.

3D structure databases

ProteinModelPortaliP48726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi412030.XP_001438693.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiCAK71296; CAK71296; GSPATT00001010001.
GeneIDi5024478.
KEGGiptm:GSPATT00001010001.

Phylogenomic databases

eggNOGiKOG0371. Eukaryota.
COG0639. LUCA.
InParanoidiP48726.
KOiK04382.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP2A1_PARTE
AccessioniPrimary (citable) accession number: P48726
Secondary accession number(s): A0CKH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 23, 2007
Last modified: November 30, 2016
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.