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P48725

- PCNT_MOUSE

UniProt

P48725 - PCNT_MOUSE

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Protein
Pericentrin
Gene
Pcnt, Pcnt2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome By similarity.1 Publication

GO - Molecular functioni

  1. protein binding Source: UniProtKB

GO - Biological processi

  1. brain morphogenesis Source: MGI
  2. cerebellar cortex morphogenesis Source: MGI
  3. in utero embryonic development Source: MGI
  4. limb morphogenesis Source: MGI
  5. microtubule cytoskeleton organization Source: UniProtKB
  6. multicellular organism growth Source: MGI
  7. negative regulation of apoptotic process Source: MGI
  8. neural precursor cell proliferation Source: MGI
  9. neuron migration Source: MGI
  10. olfactory bulb development Source: MGI
  11. positive regulation of intracellular protein transport Source: UniProtKB
  12. spindle organization Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pericentrin
Gene namesi
Name:Pcnt
Synonyms:Pcnt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:102722. Pcnt.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome By similarity.1 Publication

GO - Cellular componenti

  1. centriolar satellite Source: UniProtKB
  2. centriole Source: MGI
  3. centrosome Source: UniProtKB
  4. ciliary basal body Source: MGI
  5. intercellular bridge Source: MGI
  6. microtubule Source: UniProtKB-KW
  7. microtubule organizing center Source: MGI
  8. motile cilium Source: MGI
  9. pericentriolar material Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 28982898Pericentrin
PRO_0000058258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1437 – 14371Phosphoserine1 Publication
Modified residuei1959 – 19591Phosphoserine By similarity
Modified residuei2128 – 21281Phosphoserine By similarity
Modified residuei2865 – 28651Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48725.
PRIDEiP48725.

PTM databases

PhosphoSiteiP48725.

Expressioni

Tissue specificityi

Expressed in heart and lung (at protein level). Expressed in kidney, thymus, liver, brain, muscle, testis, spleen, lung and heart.1 Publication

Developmental stagei

Expressed in embryo at 17 dpc (at protein level). Expressed in embryos at 7, 11, 15 and 17 dpc. Strongly expressed in brain tissues at early stages of development.2 Publications

Gene expression databases

CleanExiMM_PCNT.
GenevestigatoriP48725.

Interactioni

Subunit structurei

Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CEP131 By similarity. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of PCNT and CDK5RAP2. Interacts with CHD3. Interacts with CHD4; the interaction regulates centrosome integrity. Interacts with NEK2 By similarity.2 Publications

Protein-protein interaction databases

BioGridi202052. 2 interactions.
IntActiP48725. 1 interaction.
MINTiMINT-4084690.

Structurei

3D structure databases

ProteinModelPortaliP48725.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1801 – 182222Interaction with CDK5RAP2
Add
BLAST
Regioni2545 – 2810266Interaction with NEK2 By similarity
Add
BLAST
Regioni2758 – 277114Calmodulin-binding By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili127 – 343217 Reviewed prediction
Add
BLAST
Coiled coili382 – 43453 Reviewed prediction
Add
BLAST
Coiled coili468 – 52760 Reviewed prediction
Add
BLAST
Coiled coili611 – 69686 Reviewed prediction
Add
BLAST
Coiled coili727 – 78761 Reviewed prediction
Add
BLAST
Coiled coili872 – 93968 Reviewed prediction
Add
BLAST
Coiled coili1069 – 1383315 Reviewed prediction
Add
BLAST
Coiled coili1429 – 148254 Reviewed prediction
Add
BLAST
Coiled coili1529 – 159365 Reviewed prediction
Add
BLAST
Coiled coili2211 – 2403193 Reviewed prediction
Add
BLAST
Coiled coili2429 – 2590162 Reviewed prediction
Add
BLAST

Domaini

Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000168229.
HOVERGENiHBG079443.
InParanoidiA0JBT1.
KOiK16481.
PhylomeDBiP48725.

Family and domain databases

InterProiIPR019528. PACT_domain.
IPR024151. Pericentrin.
[Graphical view]
PANTHERiPTHR18932:SF11. PTHR18932:SF11. 1 hit.
PfamiPF10495. PACT_coil_coil. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P48725-1) [UniParc]FASTAAdd to Basket

Also known as: Pericentrin B, Pericentrin-360

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEDEQEQRRR KVEAGRAKLA NFRQRKTKGD CPNSKKKTAK RKGSAVHASV     50
QEEGSVATPN SELPQGGAVF ESPSCSNTLE GTRGASAAQE QEDCELDVTD 100
LQGQQQTQPP PPQTAHSLEL EALRLSLNNM HTAQLELTQA NLQKEKETAL 150
TELREMLNGR RAQELALLQS RQQCELELLR EQHAREKEEM ALRSGQEAAE 200
LKEKLRSEME KNAQTIETLK QDWESERELC LENLRQELSL KHQSEMEGLQ 250
SQFQKELSEQ KVELEKIFQA KHEAEVSLKN LEAQHQAAIK KLQEDLQSEH 300
CQYLQDLEQK FREKEKAKEL ELETLQASYE DLKAQSQEEI RLLWSQLESM 350
KTNREELNGS WDPVLAQASH LEELEHLRSG FAQQQQQERA QHESELEHLR 400
VYFEKKLKDA EKTYQEDLTV FQQRLQEARE DSLESTEISS SCVLPEETSG 450
REGKEPPDPL DLQLGQPKVQ ESLVEDCQVK LSKAEEKIQQ MKEEFQKKEA 500
EWELSREELK REAEERLASM FLELREKAES EKLSIISRFE HRESSMRHLQ 550
DQQAAQILDL ERSLMEQQGH LRQLEQELTR DDLLPCSQCG QEPAMAQEEK 600
NGALLREKED CALQLLMAQN RFLEERKEIM EKFAKEQDAF LRDAQEKHNH 650
ELQLLQQGHQ QQLLALRMEL ETKHRSELTE QLASSESRRQ ALLETHVAEL 700
QVKHNAEISA LEKRHLSNLD ELESCYVADV QTIRDEHQQA LELLRAELEE 750
QLQKKESCHR EMLTQELENL KRQHAEELQS VRDSLRMEMS AQHIENGKGP 800
AADLQGAHQQ DPAMALHNEG HLLVEDGDAV LRSVDAEGLL HQAGPQELGD 850
AHTVEMQKSQ AELAKPQELQ ASQDQVAQVR DKVFLLNREL EECRAELEQL 900
QQRRERENQE GTTLICMLRA DLELAQGEGK ALRDALRRLL DLFGDTLKAA 950
VTLKSRISER AGLLLDHEDA ADTSDARLAA AALGDMWSDE GLLEIDRTLP 1000
EGAETSSVCE ISSHVCESFF ISPENTLDCE QPIRRVYQSL STAVEGLLEM 1050
ALDSSKQLEE ARQLHRCVER EFRHRNEEMA QAMQKQQELL ERLREESAAK 1100
DRLALELHTA KGLLEGFKVE KVDLQEALGK KEESEQQLIL ELEDLRKQLQ 1150
QAARELLTLK EEKSVLWNQK ETLTNEAKER EAALQEEVES LTRVQWESRK 1200
QSEKDRATLL SQMRVLESEL EDQLVQHRGC AQLAEEVATL KQQLAALDKH 1250
LRSQRQFMDD QAAEREHERE EFQQEIQRLE GQLRQAARPR PPGPRDSQCV 1300
QLDEEVELLQ EKLREKLDGF NELVIKKDFA DQQLLIQEEE IKRLEETNAS 1350
IQRQMVQLQE ELEKQKKSME ELKEKEILKQ ENMGDLLLTT VSRSGLDEAG 1400
CPMLPQGSSS RGPEAQPDVT ERALLQHENE VVHRRNSEID ELKSLIENLQ 1450
ENQRQLQKDK AEEIEQLHEV IEKLQSELSL MGPKVHEVSD PQAGSLHSEL 1500
ACLRGEGLGG QALRSELQAA QAAKEVFGQL LANQAHGHSQ ALEALQQRLQ 1550
DAEEVAARHL AELEHCVALR EAEVEAMASQ IQEFAATLKA KEAIIEQRDL 1600
EIDAVNKWKV SHSLELEAIL LALAHFRHAL EQQTCATPDE PPELRQLRVQ 1650
CARLSHQLQV LYRPFLKCRM QLDQHQPHVA SIGCANPCAD DELEQEGVSN 1700
RLALAPHSLA AQAKEELEDC PLGKANLMAQ VRQLQEELDH RVHSVASRDT 1750
NSETCKLQQP NLSENGPRNH CCNGEESKPS PPDDVLNIAK TTWDVIDIIK 1800
NQDLLVQVEM PDFPTQEKLT SQGGPFSSQA SGHSGSLLPE EAAEPQQDPV 1850
RALDLSSWSS PEVVRKDPSL EPQHSLPLTP GVGTVSLHSV DISPDWTDPL 1900
LQADVSGLLC YPGKSASGQA PLWAVAPSAG KHHAERTATE KDVEDFIVTS 1950
FDSQELLTSP SHELARRSDG SRKSDGPDIA MMLTLGSEGS ETPTTDLVAA 2000
AAAAVPFSRR FVQSPGAMKE KEIHAKQMKA LLQMVFDESH QILALSESQD 2050
PSSALNKGEP RDPLDGFPRD SQALSEVTTD KGEKESLETH LTWSEELLRA 2100
IQEVFAREQE KAELQPRPYG SNLGDYNSLV QRLEKVIQEQ GDPQKVQDHL 2150
CLSDRSSLLA EIQALRAQLR MTHLQNQEKL QQLCAALTST EARGSQREHQ 2200
LRRQVELLAY KVEQEKCIAN ELQKTLSKEQ ETASDVRKRL VVEQNAVQDL 2250
KSELHACKQE NTSLLESLDK VQQEVLRLRA VLDGKEKELK VVLEELESER 2300
GKGQALQAQQ EEQQLRYLQR EGQSSRALEE LKLSLEKQLA QNNQLCVALK 2350
HERAAKDNLQ KELQIEASRC EALLAQEKGQ LSELQKSLEA ERSRSLELSE 2400
ALQHERLLTE QLSRNSQEAC ARQETQVQHA LLRKLKAEKT RALELEAMLE 2450
KVQKQAAHTQ QQLEAQAQER CVELRREKER ELEIQRQRDE HKIEQLQRLV 2500
RELRWKEEVS GGNGPCRGSP GRGSLERDQF QEQQQELEKI RQQLLCAAGL 2550
LTSFTNHTVD RTIKDWTSSN EKAVSSLMRT LEELKSELSM PTSFQKKMTA 2600
ELQVQLMNEL LSDNDALTKA VGMATREKAE LCRTVSRLEK TLKHHTQKGC 2650
VLNRQSKSSL KQDGTDLQSS LRHSDPEWHS QTTSGDTNTC NIKMEKLYLH 2700
YLRAESFRKA LIYQKKYLLL LIGGFQDSEQ ETLSMIAHLG VFPSKADKKI 2750
TMSRPFTKFR TAVRVVIAVL RLRFLVKKWQ EVDRKGALVH PKSTRHGHRT 2800
SQRQRSPSGP RASLPTRDTS SGPTKASRHS PRSAAAGSPG KERSTSTPSS 2850
RLERSLTASQ DPEHSLTEYI HHLEMIQQRL GGLPPDSTQK SCHQKIKQ 2898
Length:2,898
Mass (Da):329,465
Last modified:April 20, 2010 - v2
Checksum:i50E66D93F7E4E283
GO
Isoform 2 (identifier: P48725-2) [UniParc]FASTAAdd to Basket

Also known as: Pericentrin S, Pericentrin-255

The sequence of this isoform differs from the canonical sequence as follows:
     1-916: Missing.

Show »
Length:1,982
Mass (Da):223,682
Checksum:i2781F4B76DB80818
GO
Isoform 3 (identifier: P48725-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2044-2050: ALSESQD → GNSRLLF
     2501-2898: Missing.

Note: No experimental confirmation available.

Show »
Length:2,500
Mass (Da):284,589
Checksum:iF17397506865AAAE
GO

Sequence cautioni

The sequence AAA17886.1 differs from that shown. Reason: Erroneous termination at position 1923. Translated as Trp.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 916916Missing in isoform 2.
VSP_038927Add
BLAST
Alternative sequencei2044 – 20507ALSESQD → GNSRLLF in isoform 3.
VSP_038928
Alternative sequencei2501 – 2898398Missing in isoform 3.
VSP_038929Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti652 – 6521Missing in AAA17886. 1 Publication
Sequence conflicti662 – 6621Missing in AAA17886. 1 Publication
Sequence conflicti896 – 8972EL → DV in AAA17886. 1 Publication
Sequence conflicti960 – 9601R → G in AAA17886. 1 Publication
Sequence conflicti982 – 9821A → P in AAA17886. 1 Publication
Sequence conflicti1022 – 10221S → R in AAA17886. 1 Publication
Sequence conflicti1150 – 11501Q → E in AAA17886. 1 Publication
Sequence conflicti1266 – 12672EH → DD in AAA17886. 1 Publication
Sequence conflicti1533 – 15331N → D in AAA17886. 1 Publication
Sequence conflicti1548 – 15481R → G in AAA17886. 1 Publication
Sequence conflicti1726 – 17261N → G in AAA17886. 1 Publication
Sequence conflicti1736 – 17361E → Q in AAA17886. 1 Publication
Sequence conflicti1772 – 17721C → S in AAA17886. 1 Publication
Sequence conflicti2040 – 20401H → L in AAA17886. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05823 mRNA. Translation: AAA17886.1. Sequence problems.
AB207233 mRNA. Translation: BAF36559.1.
AB207234 mRNA. Translation: BAF36560.1.
PIRiA53188.
RefSeqiNP_001269921.1. NM_001282992.1.
NP_032813.3. NM_008787.3.
UniGeneiMm.251794.

Genome annotation databases

GeneIDi18541.
KEGGimmu:18541.
UCSCiuc007fuj.1. mouse. [P48725-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05823 mRNA. Translation: AAA17886.1 . Sequence problems.
AB207233 mRNA. Translation: BAF36559.1 .
AB207234 mRNA. Translation: BAF36560.1 .
PIRi A53188.
RefSeqi NP_001269921.1. NM_001282992.1.
NP_032813.3. NM_008787.3.
UniGenei Mm.251794.

3D structure databases

ProteinModelPortali P48725.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202052. 2 interactions.
IntActi P48725. 1 interaction.
MINTi MINT-4084690.

PTM databases

PhosphoSitei P48725.

Proteomic databases

PaxDbi P48725.
PRIDEi P48725.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 18541.
KEGGi mmu:18541.
UCSCi uc007fuj.1. mouse. [P48725-1 ]

Organism-specific databases

CTDi 5116.
MGIi MGI:102722. Pcnt.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000168229.
HOVERGENi HBG079443.
InParanoidi A0JBT1.
KOi K16481.
PhylomeDBi P48725.

Miscellaneous databases

NextBioi 294320.
PROi P48725.
SOURCEi Search...

Gene expression databases

CleanExi MM_PCNT.
Genevestigatori P48725.

Family and domain databases

InterProi IPR019528. PACT_domain.
IPR024151. Pericentrin.
[Graphical view ]
PANTHERi PTHR18932:SF11. PTHR18932:SF11. 1 hit.
Pfami PF10495. PACT_coil_coil. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pericentrin, a highly conserved centrosome protein involved in microtubule organization."
    Doxsey S.J., Stein P., Evans L., Calarco P.D., Kirschner M.
    Cell 76:639-650(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  3. "Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity."
    Sillibourne J.E., Delaval B., Redick S., Sinha M., Doxsey S.J.
    Mol. Biol. Cell 18:3667-3680(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHD3 AND CHD4, SUBCELLULAR LOCATION, FUNCTION.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1437, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Cdk5rap2 interacts with pericentrin to maintain the neural progenitor pool in the developing neocortex."
    Buchman J.J., Tseng H.C., Zhou Y., Frank C.L., Xie Z., Tsai L.H.
    Neuron 66:386-402(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDK5RAP2, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPCNT_MOUSE
AccessioniPrimary (citable) accession number: P48725
Secondary accession number(s): A0JBT0, A0JBT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 20, 2010
Last modified: June 11, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

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