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P48725

- PCNT_MOUSE

UniProt

P48725 - PCNT_MOUSE

Protein

Pericentrin

Gene

Pcnt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. brain morphogenesis Source: MGI
    2. cerebellar cortex morphogenesis Source: MGI
    3. in utero embryonic development Source: MGI
    4. limb morphogenesis Source: MGI
    5. microtubule cytoskeleton organization Source: UniProtKB
    6. multicellular organism growth Source: MGI
    7. negative regulation of apoptotic process Source: MGI
    8. neural precursor cell proliferation Source: MGI
    9. neuron migration Source: MGI
    10. olfactory bulb development Source: MGI
    11. positive regulation of intracellular protein transport Source: UniProtKB
    12. spindle organization Source: MGI

    Keywords - Ligandi

    Calmodulin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pericentrin
    Gene namesi
    Name:Pcnt
    Synonyms:Pcnt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:102722. Pcnt.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
    Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome By similarity.By similarity

    GO - Cellular componenti

    1. centriolar satellite Source: UniProtKB
    2. centriole Source: MGI
    3. centrosome Source: UniProtKB
    4. ciliary basal body Source: MGI
    5. intercellular bridge Source: MGI
    6. microtubule Source: UniProtKB-KW
    7. microtubule organizing center Source: MGI
    8. motile cilium Source: MGI
    9. pericentriolar material Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 28982898PericentrinPRO_0000058258Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1437 – 14371Phosphoserine1 Publication
    Modified residuei1959 – 19591PhosphoserineBy similarity
    Modified residuei2128 – 21281PhosphoserineBy similarity
    Modified residuei2865 – 28651PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP48725.
    PRIDEiP48725.

    PTM databases

    PhosphoSiteiP48725.

    Expressioni

    Tissue specificityi

    Expressed in heart and lung (at protein level). Expressed in kidney, thymus, liver, brain, muscle, testis, spleen, lung and heart.1 Publication

    Developmental stagei

    Expressed in embryo at 17 dpc (at protein level). Expressed in embryos at 7, 11, 15 and 17 dpc. Strongly expressed in brain tissues at early stages of development.2 Publications

    Gene expression databases

    CleanExiMM_PCNT.
    GenevestigatoriP48725.

    Interactioni

    Subunit structurei

    Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CEP131 By similarity. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of PCNT and CDK5RAP2. Interacts with CHD3. Interacts with CHD4; the interaction regulates centrosome integrity. Interacts with NEK2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202052. 2 interactions.
    IntActiP48725. 1 interaction.
    MINTiMINT-4084690.

    Structurei

    3D structure databases

    ProteinModelPortaliP48725.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1801 – 182222Interaction with CDK5RAP2Add
    BLAST
    Regioni2545 – 2810266Interaction with NEK2By similarityAdd
    BLAST
    Regioni2758 – 277114Calmodulin-bindingBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili127 – 343217Sequence AnalysisAdd
    BLAST
    Coiled coili382 – 43453Sequence AnalysisAdd
    BLAST
    Coiled coili468 – 52760Sequence AnalysisAdd
    BLAST
    Coiled coili611 – 69686Sequence AnalysisAdd
    BLAST
    Coiled coili727 – 78761Sequence AnalysisAdd
    BLAST
    Coiled coili872 – 93968Sequence AnalysisAdd
    BLAST
    Coiled coili1069 – 1383315Sequence AnalysisAdd
    BLAST
    Coiled coili1429 – 148254Sequence AnalysisAdd
    BLAST
    Coiled coili1529 – 159365Sequence AnalysisAdd
    BLAST
    Coiled coili2211 – 2403193Sequence AnalysisAdd
    BLAST
    Coiled coili2429 – 2590162Sequence AnalysisAdd
    BLAST

    Domaini

    Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000168229.
    HOVERGENiHBG079443.
    InParanoidiA0JBT1.
    KOiK16481.
    PhylomeDBiP48725.

    Family and domain databases

    InterProiIPR019528. PACT_domain.
    IPR024151. Pericentrin.
    [Graphical view]
    PANTHERiPTHR18932:SF11. PTHR18932:SF11. 1 hit.
    PfamiPF10495. PACT_coil_coil. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P48725-1) [UniParc]FASTAAdd to Basket

    Also known as: Pericentrin B, Pericentrin-360

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEDEQEQRRR KVEAGRAKLA NFRQRKTKGD CPNSKKKTAK RKGSAVHASV     50
    QEEGSVATPN SELPQGGAVF ESPSCSNTLE GTRGASAAQE QEDCELDVTD 100
    LQGQQQTQPP PPQTAHSLEL EALRLSLNNM HTAQLELTQA NLQKEKETAL 150
    TELREMLNGR RAQELALLQS RQQCELELLR EQHAREKEEM ALRSGQEAAE 200
    LKEKLRSEME KNAQTIETLK QDWESERELC LENLRQELSL KHQSEMEGLQ 250
    SQFQKELSEQ KVELEKIFQA KHEAEVSLKN LEAQHQAAIK KLQEDLQSEH 300
    CQYLQDLEQK FREKEKAKEL ELETLQASYE DLKAQSQEEI RLLWSQLESM 350
    KTNREELNGS WDPVLAQASH LEELEHLRSG FAQQQQQERA QHESELEHLR 400
    VYFEKKLKDA EKTYQEDLTV FQQRLQEARE DSLESTEISS SCVLPEETSG 450
    REGKEPPDPL DLQLGQPKVQ ESLVEDCQVK LSKAEEKIQQ MKEEFQKKEA 500
    EWELSREELK REAEERLASM FLELREKAES EKLSIISRFE HRESSMRHLQ 550
    DQQAAQILDL ERSLMEQQGH LRQLEQELTR DDLLPCSQCG QEPAMAQEEK 600
    NGALLREKED CALQLLMAQN RFLEERKEIM EKFAKEQDAF LRDAQEKHNH 650
    ELQLLQQGHQ QQLLALRMEL ETKHRSELTE QLASSESRRQ ALLETHVAEL 700
    QVKHNAEISA LEKRHLSNLD ELESCYVADV QTIRDEHQQA LELLRAELEE 750
    QLQKKESCHR EMLTQELENL KRQHAEELQS VRDSLRMEMS AQHIENGKGP 800
    AADLQGAHQQ DPAMALHNEG HLLVEDGDAV LRSVDAEGLL HQAGPQELGD 850
    AHTVEMQKSQ AELAKPQELQ ASQDQVAQVR DKVFLLNREL EECRAELEQL 900
    QQRRERENQE GTTLICMLRA DLELAQGEGK ALRDALRRLL DLFGDTLKAA 950
    VTLKSRISER AGLLLDHEDA ADTSDARLAA AALGDMWSDE GLLEIDRTLP 1000
    EGAETSSVCE ISSHVCESFF ISPENTLDCE QPIRRVYQSL STAVEGLLEM 1050
    ALDSSKQLEE ARQLHRCVER EFRHRNEEMA QAMQKQQELL ERLREESAAK 1100
    DRLALELHTA KGLLEGFKVE KVDLQEALGK KEESEQQLIL ELEDLRKQLQ 1150
    QAARELLTLK EEKSVLWNQK ETLTNEAKER EAALQEEVES LTRVQWESRK 1200
    QSEKDRATLL SQMRVLESEL EDQLVQHRGC AQLAEEVATL KQQLAALDKH 1250
    LRSQRQFMDD QAAEREHERE EFQQEIQRLE GQLRQAARPR PPGPRDSQCV 1300
    QLDEEVELLQ EKLREKLDGF NELVIKKDFA DQQLLIQEEE IKRLEETNAS 1350
    IQRQMVQLQE ELEKQKKSME ELKEKEILKQ ENMGDLLLTT VSRSGLDEAG 1400
    CPMLPQGSSS RGPEAQPDVT ERALLQHENE VVHRRNSEID ELKSLIENLQ 1450
    ENQRQLQKDK AEEIEQLHEV IEKLQSELSL MGPKVHEVSD PQAGSLHSEL 1500
    ACLRGEGLGG QALRSELQAA QAAKEVFGQL LANQAHGHSQ ALEALQQRLQ 1550
    DAEEVAARHL AELEHCVALR EAEVEAMASQ IQEFAATLKA KEAIIEQRDL 1600
    EIDAVNKWKV SHSLELEAIL LALAHFRHAL EQQTCATPDE PPELRQLRVQ 1650
    CARLSHQLQV LYRPFLKCRM QLDQHQPHVA SIGCANPCAD DELEQEGVSN 1700
    RLALAPHSLA AQAKEELEDC PLGKANLMAQ VRQLQEELDH RVHSVASRDT 1750
    NSETCKLQQP NLSENGPRNH CCNGEESKPS PPDDVLNIAK TTWDVIDIIK 1800
    NQDLLVQVEM PDFPTQEKLT SQGGPFSSQA SGHSGSLLPE EAAEPQQDPV 1850
    RALDLSSWSS PEVVRKDPSL EPQHSLPLTP GVGTVSLHSV DISPDWTDPL 1900
    LQADVSGLLC YPGKSASGQA PLWAVAPSAG KHHAERTATE KDVEDFIVTS 1950
    FDSQELLTSP SHELARRSDG SRKSDGPDIA MMLTLGSEGS ETPTTDLVAA 2000
    AAAAVPFSRR FVQSPGAMKE KEIHAKQMKA LLQMVFDESH QILALSESQD 2050
    PSSALNKGEP RDPLDGFPRD SQALSEVTTD KGEKESLETH LTWSEELLRA 2100
    IQEVFAREQE KAELQPRPYG SNLGDYNSLV QRLEKVIQEQ GDPQKVQDHL 2150
    CLSDRSSLLA EIQALRAQLR MTHLQNQEKL QQLCAALTST EARGSQREHQ 2200
    LRRQVELLAY KVEQEKCIAN ELQKTLSKEQ ETASDVRKRL VVEQNAVQDL 2250
    KSELHACKQE NTSLLESLDK VQQEVLRLRA VLDGKEKELK VVLEELESER 2300
    GKGQALQAQQ EEQQLRYLQR EGQSSRALEE LKLSLEKQLA QNNQLCVALK 2350
    HERAAKDNLQ KELQIEASRC EALLAQEKGQ LSELQKSLEA ERSRSLELSE 2400
    ALQHERLLTE QLSRNSQEAC ARQETQVQHA LLRKLKAEKT RALELEAMLE 2450
    KVQKQAAHTQ QQLEAQAQER CVELRREKER ELEIQRQRDE HKIEQLQRLV 2500
    RELRWKEEVS GGNGPCRGSP GRGSLERDQF QEQQQELEKI RQQLLCAAGL 2550
    LTSFTNHTVD RTIKDWTSSN EKAVSSLMRT LEELKSELSM PTSFQKKMTA 2600
    ELQVQLMNEL LSDNDALTKA VGMATREKAE LCRTVSRLEK TLKHHTQKGC 2650
    VLNRQSKSSL KQDGTDLQSS LRHSDPEWHS QTTSGDTNTC NIKMEKLYLH 2700
    YLRAESFRKA LIYQKKYLLL LIGGFQDSEQ ETLSMIAHLG VFPSKADKKI 2750
    TMSRPFTKFR TAVRVVIAVL RLRFLVKKWQ EVDRKGALVH PKSTRHGHRT 2800
    SQRQRSPSGP RASLPTRDTS SGPTKASRHS PRSAAAGSPG KERSTSTPSS 2850
    RLERSLTASQ DPEHSLTEYI HHLEMIQQRL GGLPPDSTQK SCHQKIKQ 2898
    Length:2,898
    Mass (Da):329,465
    Last modified:April 20, 2010 - v2
    Checksum:i50E66D93F7E4E283
    GO
    Isoform 2 (identifier: P48725-2) [UniParc]FASTAAdd to Basket

    Also known as: Pericentrin S, Pericentrin-255

    The sequence of this isoform differs from the canonical sequence as follows:
         1-916: Missing.

    Show »
    Length:1,982
    Mass (Da):223,682
    Checksum:i2781F4B76DB80818
    GO
    Isoform 3 (identifier: P48725-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2044-2050: ALSESQD → GNSRLLF
         2501-2898: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:2,500
    Mass (Da):284,589
    Checksum:iF17397506865AAAE
    GO

    Sequence cautioni

    The sequence AAA17886.1 differs from that shown. Reason: Erroneous termination at position 1923. Translated as Trp.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti652 – 6521Missing in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti662 – 6621Missing in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti896 – 8972EL → DV in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti960 – 9601R → G in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti982 – 9821A → P in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti1022 – 10221S → R in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti1150 – 11501Q → E in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti1266 – 12672EH → DD in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti1533 – 15331N → D in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti1548 – 15481R → G in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti1726 – 17261N → G in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti1736 – 17361E → Q in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti1772 – 17721C → S in AAA17886. (PubMed:8124707)Curated
    Sequence conflicti2040 – 20401H → L in AAA17886. (PubMed:8124707)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 916916Missing in isoform 2. 1 PublicationVSP_038927Add
    BLAST
    Alternative sequencei2044 – 20507ALSESQD → GNSRLLF in isoform 3. 1 PublicationVSP_038928
    Alternative sequencei2501 – 2898398Missing in isoform 3. 1 PublicationVSP_038929Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05823 mRNA. Translation: AAA17886.1. Sequence problems.
    AB207233 mRNA. Translation: BAF36559.1.
    AB207234 mRNA. Translation: BAF36560.1.
    PIRiA53188.
    RefSeqiNP_001269921.1. NM_001282992.1.
    NP_032813.3. NM_008787.3.
    UniGeneiMm.251794.

    Genome annotation databases

    GeneIDi18541.
    KEGGimmu:18541.
    UCSCiuc007fuj.1. mouse. [P48725-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05823 mRNA. Translation: AAA17886.1 . Sequence problems.
    AB207233 mRNA. Translation: BAF36559.1 .
    AB207234 mRNA. Translation: BAF36560.1 .
    PIRi A53188.
    RefSeqi NP_001269921.1. NM_001282992.1.
    NP_032813.3. NM_008787.3.
    UniGenei Mm.251794.

    3D structure databases

    ProteinModelPortali P48725.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202052. 2 interactions.
    IntActi P48725. 1 interaction.
    MINTi MINT-4084690.

    PTM databases

    PhosphoSitei P48725.

    Proteomic databases

    PaxDbi P48725.
    PRIDEi P48725.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 18541.
    KEGGi mmu:18541.
    UCSCi uc007fuj.1. mouse. [P48725-1 ]

    Organism-specific databases

    CTDi 5116.
    MGIi MGI:102722. Pcnt.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000168229.
    HOVERGENi HBG079443.
    InParanoidi A0JBT1.
    KOi K16481.
    PhylomeDBi P48725.

    Miscellaneous databases

    NextBioi 294320.
    PROi P48725.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_PCNT.
    Genevestigatori P48725.

    Family and domain databases

    InterProi IPR019528. PACT_domain.
    IPR024151. Pericentrin.
    [Graphical view ]
    PANTHERi PTHR18932:SF11. PTHR18932:SF11. 1 hit.
    Pfami PF10495. PACT_coil_coil. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Pericentrin, a highly conserved centrosome protein involved in microtubule organization."
      Doxsey S.J., Stein P., Evans L., Calarco P.D., Kirschner M.
      Cell 76:639-650(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Embryo.
    3. "Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity."
      Sillibourne J.E., Delaval B., Redick S., Sinha M., Doxsey S.J.
      Mol. Biol. Cell 18:3667-3680(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHD3 AND CHD4, SUBCELLULAR LOCATION, FUNCTION.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1437, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Cdk5rap2 interacts with pericentrin to maintain the neural progenitor pool in the developing neocortex."
      Buchman J.J., Tseng H.C., Zhou Y., Frank C.L., Xie Z., Tsai L.H.
      Neuron 66:386-402(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDK5RAP2, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiPCNT_MOUSE
    AccessioniPrimary (citable) accession number: P48725
    Secondary accession number(s): A0JBT0, A0JBT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3