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P48725

- PCNT_MOUSE

UniProt

P48725 - PCNT_MOUSE

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Protein

Pericentrin

Gene

Pcnt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome (By similarity).By similarity

GO - Biological processi

  1. brain morphogenesis Source: MGI
  2. cerebellar cortex morphogenesis Source: MGI
  3. in utero embryonic development Source: MGI
  4. limb morphogenesis Source: MGI
  5. microtubule cytoskeleton organization Source: UniProtKB
  6. multicellular organism growth Source: MGI
  7. negative regulation of apoptotic process Source: MGI
  8. neural precursor cell proliferation Source: MGI
  9. neuron migration Source: MGI
  10. olfactory bulb development Source: MGI
  11. positive regulation of intracellular protein transport Source: UniProtKB
  12. spindle organization Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pericentrin
Gene namesi
Name:Pcnt
Synonyms:Pcnt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:102722. Pcnt.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome (By similarity).By similarity

GO - Cellular componenti

  1. centriolar satellite Source: UniProtKB
  2. centriole Source: MGI
  3. centrosome Source: UniProtKB
  4. ciliary basal body Source: MGI
  5. intercellular bridge Source: MGI
  6. microtubule Source: UniProtKB-KW
  7. microtubule organizing center Source: MGI
  8. motile cilium Source: MGI
  9. pericentriolar material Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 28982898PericentrinPRO_0000058258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1437 – 14371Phosphoserine1 Publication
Modified residuei1959 – 19591PhosphoserineBy similarity
Modified residuei2128 – 21281PhosphoserineBy similarity
Modified residuei2865 – 28651PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48725.
PaxDbiP48725.
PRIDEiP48725.

PTM databases

PhosphoSiteiP48725.

Expressioni

Tissue specificityi

Expressed in heart and lung (at protein level). Expressed in kidney, thymus, liver, brain, muscle, testis, spleen, lung and heart.1 Publication

Developmental stagei

Expressed in embryo at 17 dpc (at protein level). Expressed in embryos at 7, 11, 15 and 17 dpc. Strongly expressed in brain tissues at early stages of development.2 Publications

Gene expression databases

CleanExiMM_PCNT.
GenevestigatoriP48725.

Interactioni

Subunit structurei

Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CEP131 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of PCNT and CDK5RAP2. Interacts with CHD3. Interacts with CHD4; the interaction regulates centrosome integrity. Interacts with NEK2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi202052. 2 interactions.
IntActiP48725. 1 interaction.
MINTiMINT-4084690.

Structurei

3D structure databases

ProteinModelPortaliP48725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1801 – 182222Interaction with CDK5RAP2Add
BLAST
Regioni2545 – 2810266Interaction with NEK2By similarityAdd
BLAST
Regioni2758 – 277114Calmodulin-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili127 – 343217Sequence AnalysisAdd
BLAST
Coiled coili382 – 43453Sequence AnalysisAdd
BLAST
Coiled coili468 – 52760Sequence AnalysisAdd
BLAST
Coiled coili611 – 69686Sequence AnalysisAdd
BLAST
Coiled coili727 – 78761Sequence AnalysisAdd
BLAST
Coiled coili872 – 93968Sequence AnalysisAdd
BLAST
Coiled coili1069 – 1383315Sequence AnalysisAdd
BLAST
Coiled coili1429 – 148254Sequence AnalysisAdd
BLAST
Coiled coili1529 – 159365Sequence AnalysisAdd
BLAST
Coiled coili2211 – 2403193Sequence AnalysisAdd
BLAST
Coiled coili2429 – 2590162Sequence AnalysisAdd
BLAST

Domaini

Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000168229.
HOVERGENiHBG079443.
InParanoidiP48725.
KOiK16481.
PhylomeDBiP48725.

Family and domain databases

InterProiIPR019528. PACT_domain.
IPR024151. Pericentrin.
[Graphical view]
PANTHERiPTHR18932:SF11. PTHR18932:SF11. 1 hit.
PfamiPF10495. PACT_coil_coil. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P48725-1) [UniParc]FASTAAdd to Basket

Also known as: Pericentrin B, Pericentrin-360

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDEQEQRRR KVEAGRAKLA NFRQRKTKGD CPNSKKKTAK RKGSAVHASV
60 70 80 90 100
QEEGSVATPN SELPQGGAVF ESPSCSNTLE GTRGASAAQE QEDCELDVTD
110 120 130 140 150
LQGQQQTQPP PPQTAHSLEL EALRLSLNNM HTAQLELTQA NLQKEKETAL
160 170 180 190 200
TELREMLNGR RAQELALLQS RQQCELELLR EQHAREKEEM ALRSGQEAAE
210 220 230 240 250
LKEKLRSEME KNAQTIETLK QDWESERELC LENLRQELSL KHQSEMEGLQ
260 270 280 290 300
SQFQKELSEQ KVELEKIFQA KHEAEVSLKN LEAQHQAAIK KLQEDLQSEH
310 320 330 340 350
CQYLQDLEQK FREKEKAKEL ELETLQASYE DLKAQSQEEI RLLWSQLESM
360 370 380 390 400
KTNREELNGS WDPVLAQASH LEELEHLRSG FAQQQQQERA QHESELEHLR
410 420 430 440 450
VYFEKKLKDA EKTYQEDLTV FQQRLQEARE DSLESTEISS SCVLPEETSG
460 470 480 490 500
REGKEPPDPL DLQLGQPKVQ ESLVEDCQVK LSKAEEKIQQ MKEEFQKKEA
510 520 530 540 550
EWELSREELK REAEERLASM FLELREKAES EKLSIISRFE HRESSMRHLQ
560 570 580 590 600
DQQAAQILDL ERSLMEQQGH LRQLEQELTR DDLLPCSQCG QEPAMAQEEK
610 620 630 640 650
NGALLREKED CALQLLMAQN RFLEERKEIM EKFAKEQDAF LRDAQEKHNH
660 670 680 690 700
ELQLLQQGHQ QQLLALRMEL ETKHRSELTE QLASSESRRQ ALLETHVAEL
710 720 730 740 750
QVKHNAEISA LEKRHLSNLD ELESCYVADV QTIRDEHQQA LELLRAELEE
760 770 780 790 800
QLQKKESCHR EMLTQELENL KRQHAEELQS VRDSLRMEMS AQHIENGKGP
810 820 830 840 850
AADLQGAHQQ DPAMALHNEG HLLVEDGDAV LRSVDAEGLL HQAGPQELGD
860 870 880 890 900
AHTVEMQKSQ AELAKPQELQ ASQDQVAQVR DKVFLLNREL EECRAELEQL
910 920 930 940 950
QQRRERENQE GTTLICMLRA DLELAQGEGK ALRDALRRLL DLFGDTLKAA
960 970 980 990 1000
VTLKSRISER AGLLLDHEDA ADTSDARLAA AALGDMWSDE GLLEIDRTLP
1010 1020 1030 1040 1050
EGAETSSVCE ISSHVCESFF ISPENTLDCE QPIRRVYQSL STAVEGLLEM
1060 1070 1080 1090 1100
ALDSSKQLEE ARQLHRCVER EFRHRNEEMA QAMQKQQELL ERLREESAAK
1110 1120 1130 1140 1150
DRLALELHTA KGLLEGFKVE KVDLQEALGK KEESEQQLIL ELEDLRKQLQ
1160 1170 1180 1190 1200
QAARELLTLK EEKSVLWNQK ETLTNEAKER EAALQEEVES LTRVQWESRK
1210 1220 1230 1240 1250
QSEKDRATLL SQMRVLESEL EDQLVQHRGC AQLAEEVATL KQQLAALDKH
1260 1270 1280 1290 1300
LRSQRQFMDD QAAEREHERE EFQQEIQRLE GQLRQAARPR PPGPRDSQCV
1310 1320 1330 1340 1350
QLDEEVELLQ EKLREKLDGF NELVIKKDFA DQQLLIQEEE IKRLEETNAS
1360 1370 1380 1390 1400
IQRQMVQLQE ELEKQKKSME ELKEKEILKQ ENMGDLLLTT VSRSGLDEAG
1410 1420 1430 1440 1450
CPMLPQGSSS RGPEAQPDVT ERALLQHENE VVHRRNSEID ELKSLIENLQ
1460 1470 1480 1490 1500
ENQRQLQKDK AEEIEQLHEV IEKLQSELSL MGPKVHEVSD PQAGSLHSEL
1510 1520 1530 1540 1550
ACLRGEGLGG QALRSELQAA QAAKEVFGQL LANQAHGHSQ ALEALQQRLQ
1560 1570 1580 1590 1600
DAEEVAARHL AELEHCVALR EAEVEAMASQ IQEFAATLKA KEAIIEQRDL
1610 1620 1630 1640 1650
EIDAVNKWKV SHSLELEAIL LALAHFRHAL EQQTCATPDE PPELRQLRVQ
1660 1670 1680 1690 1700
CARLSHQLQV LYRPFLKCRM QLDQHQPHVA SIGCANPCAD DELEQEGVSN
1710 1720 1730 1740 1750
RLALAPHSLA AQAKEELEDC PLGKANLMAQ VRQLQEELDH RVHSVASRDT
1760 1770 1780 1790 1800
NSETCKLQQP NLSENGPRNH CCNGEESKPS PPDDVLNIAK TTWDVIDIIK
1810 1820 1830 1840 1850
NQDLLVQVEM PDFPTQEKLT SQGGPFSSQA SGHSGSLLPE EAAEPQQDPV
1860 1870 1880 1890 1900
RALDLSSWSS PEVVRKDPSL EPQHSLPLTP GVGTVSLHSV DISPDWTDPL
1910 1920 1930 1940 1950
LQADVSGLLC YPGKSASGQA PLWAVAPSAG KHHAERTATE KDVEDFIVTS
1960 1970 1980 1990 2000
FDSQELLTSP SHELARRSDG SRKSDGPDIA MMLTLGSEGS ETPTTDLVAA
2010 2020 2030 2040 2050
AAAAVPFSRR FVQSPGAMKE KEIHAKQMKA LLQMVFDESH QILALSESQD
2060 2070 2080 2090 2100
PSSALNKGEP RDPLDGFPRD SQALSEVTTD KGEKESLETH LTWSEELLRA
2110 2120 2130 2140 2150
IQEVFAREQE KAELQPRPYG SNLGDYNSLV QRLEKVIQEQ GDPQKVQDHL
2160 2170 2180 2190 2200
CLSDRSSLLA EIQALRAQLR MTHLQNQEKL QQLCAALTST EARGSQREHQ
2210 2220 2230 2240 2250
LRRQVELLAY KVEQEKCIAN ELQKTLSKEQ ETASDVRKRL VVEQNAVQDL
2260 2270 2280 2290 2300
KSELHACKQE NTSLLESLDK VQQEVLRLRA VLDGKEKELK VVLEELESER
2310 2320 2330 2340 2350
GKGQALQAQQ EEQQLRYLQR EGQSSRALEE LKLSLEKQLA QNNQLCVALK
2360 2370 2380 2390 2400
HERAAKDNLQ KELQIEASRC EALLAQEKGQ LSELQKSLEA ERSRSLELSE
2410 2420 2430 2440 2450
ALQHERLLTE QLSRNSQEAC ARQETQVQHA LLRKLKAEKT RALELEAMLE
2460 2470 2480 2490 2500
KVQKQAAHTQ QQLEAQAQER CVELRREKER ELEIQRQRDE HKIEQLQRLV
2510 2520 2530 2540 2550
RELRWKEEVS GGNGPCRGSP GRGSLERDQF QEQQQELEKI RQQLLCAAGL
2560 2570 2580 2590 2600
LTSFTNHTVD RTIKDWTSSN EKAVSSLMRT LEELKSELSM PTSFQKKMTA
2610 2620 2630 2640 2650
ELQVQLMNEL LSDNDALTKA VGMATREKAE LCRTVSRLEK TLKHHTQKGC
2660 2670 2680 2690 2700
VLNRQSKSSL KQDGTDLQSS LRHSDPEWHS QTTSGDTNTC NIKMEKLYLH
2710 2720 2730 2740 2750
YLRAESFRKA LIYQKKYLLL LIGGFQDSEQ ETLSMIAHLG VFPSKADKKI
2760 2770 2780 2790 2800
TMSRPFTKFR TAVRVVIAVL RLRFLVKKWQ EVDRKGALVH PKSTRHGHRT
2810 2820 2830 2840 2850
SQRQRSPSGP RASLPTRDTS SGPTKASRHS PRSAAAGSPG KERSTSTPSS
2860 2870 2880 2890
RLERSLTASQ DPEHSLTEYI HHLEMIQQRL GGLPPDSTQK SCHQKIKQ
Length:2,898
Mass (Da):329,465
Last modified:April 20, 2010 - v2
Checksum:i50E66D93F7E4E283
GO
Isoform 2 (identifier: P48725-2) [UniParc]FASTAAdd to Basket

Also known as: Pericentrin S, Pericentrin-255

The sequence of this isoform differs from the canonical sequence as follows:
     1-916: Missing.

Show »
Length:1,982
Mass (Da):223,682
Checksum:i2781F4B76DB80818
GO
Isoform 3 (identifier: P48725-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2044-2050: ALSESQD → GNSRLLF
     2501-2898: Missing.

Note: No experimental confirmation available.

Show »
Length:2,500
Mass (Da):284,589
Checksum:iF17397506865AAAE
GO

Sequence cautioni

The sequence AAA17886.1 differs from that shown. Reason: Erroneous termination at position 1923. Translated as Trp.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti652 – 6521Missing in AAA17886. (PubMed:8124707)Curated
Sequence conflicti662 – 6621Missing in AAA17886. (PubMed:8124707)Curated
Sequence conflicti896 – 8972EL → DV in AAA17886. (PubMed:8124707)Curated
Sequence conflicti960 – 9601R → G in AAA17886. (PubMed:8124707)Curated
Sequence conflicti982 – 9821A → P in AAA17886. (PubMed:8124707)Curated
Sequence conflicti1022 – 10221S → R in AAA17886. (PubMed:8124707)Curated
Sequence conflicti1150 – 11501Q → E in AAA17886. (PubMed:8124707)Curated
Sequence conflicti1266 – 12672EH → DD in AAA17886. (PubMed:8124707)Curated
Sequence conflicti1533 – 15331N → D in AAA17886. (PubMed:8124707)Curated
Sequence conflicti1548 – 15481R → G in AAA17886. (PubMed:8124707)Curated
Sequence conflicti1726 – 17261N → G in AAA17886. (PubMed:8124707)Curated
Sequence conflicti1736 – 17361E → Q in AAA17886. (PubMed:8124707)Curated
Sequence conflicti1772 – 17721C → S in AAA17886. (PubMed:8124707)Curated
Sequence conflicti2040 – 20401H → L in AAA17886. (PubMed:8124707)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 916916Missing in isoform 2. 1 PublicationVSP_038927Add
BLAST
Alternative sequencei2044 – 20507ALSESQD → GNSRLLF in isoform 3. 1 PublicationVSP_038928
Alternative sequencei2501 – 2898398Missing in isoform 3. 1 PublicationVSP_038929Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05823 mRNA. Translation: AAA17886.1. Sequence problems.
AB207233 mRNA. Translation: BAF36559.1.
AB207234 mRNA. Translation: BAF36560.1.
PIRiA53188.
RefSeqiNP_001269921.1. NM_001282992.1.
NP_032813.3. NM_008787.3.
UniGeneiMm.251794.

Genome annotation databases

GeneIDi18541.
KEGGimmu:18541.
UCSCiuc007fuj.1. mouse. [P48725-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05823 mRNA. Translation: AAA17886.1 . Sequence problems.
AB207233 mRNA. Translation: BAF36559.1 .
AB207234 mRNA. Translation: BAF36560.1 .
PIRi A53188.
RefSeqi NP_001269921.1. NM_001282992.1.
NP_032813.3. NM_008787.3.
UniGenei Mm.251794.

3D structure databases

ProteinModelPortali P48725.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202052. 2 interactions.
IntActi P48725. 1 interaction.
MINTi MINT-4084690.

PTM databases

PhosphoSitei P48725.

Proteomic databases

MaxQBi P48725.
PaxDbi P48725.
PRIDEi P48725.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 18541.
KEGGi mmu:18541.
UCSCi uc007fuj.1. mouse. [P48725-1 ]

Organism-specific databases

CTDi 5116.
MGIi MGI:102722. Pcnt.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000168229.
HOVERGENi HBG079443.
InParanoidi P48725.
KOi K16481.
PhylomeDBi P48725.

Miscellaneous databases

NextBioi 294320.
PROi P48725.
SOURCEi Search...

Gene expression databases

CleanExi MM_PCNT.
Genevestigatori P48725.

Family and domain databases

InterProi IPR019528. PACT_domain.
IPR024151. Pericentrin.
[Graphical view ]
PANTHERi PTHR18932:SF11. PTHR18932:SF11. 1 hit.
Pfami PF10495. PACT_coil_coil. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pericentrin, a highly conserved centrosome protein involved in microtubule organization."
    Doxsey S.J., Stein P., Evans L., Calarco P.D., Kirschner M.
    Cell 76:639-650(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  3. "Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity."
    Sillibourne J.E., Delaval B., Redick S., Sinha M., Doxsey S.J.
    Mol. Biol. Cell 18:3667-3680(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHD3 AND CHD4, SUBCELLULAR LOCATION, FUNCTION.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1437, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Cdk5rap2 interacts with pericentrin to maintain the neural progenitor pool in the developing neocortex."
    Buchman J.J., Tseng H.C., Zhou Y., Frank C.L., Xie Z., Tsai L.H.
    Neuron 66:386-402(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDK5RAP2, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiPCNT_MOUSE
AccessioniPrimary (citable) accession number: P48725
Secondary accession number(s): A0JBT0, A0JBT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 20, 2010
Last modified: October 29, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3