P48722 (HS74L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock 70 kDa protein 4L

Alternative name(s):
Heat shock 70-related protein APG-1
Osmotic stress protein 94

Gene names

Name:	Hspa4l
Synonyms:	Apg1, Hsp4l, Osp94

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possesses chaperone activity in vitro where it inhibits aggregation of citrate synthase By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: May translocate to the nucleus after heat shock By similarity.
Tissue specificity	Highly expressed in testis. Also expressed in renal medulla of water-restricted animals. Ref.1
Induction	By hyperosmolar salt stress and heat shock. Ref.2
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from sequence or structural similarity. Source: UniProtKB response to unfolded protein Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P48722-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P48722-2)

Also known as: Apg-1b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MSVVGIDLGFLNCYIAVARSGGIETIANEYSDRCTP → MGGPPRHGVLDREER

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 838	838	Heat shock 70 kDa protein 4L		PRO_0000078281
Amino acid modifications
	Modified residue	74	1	Phosphoserine Ref.7
	Modified residue	761	1	Phosphothreonine Ref.8
Natural variations
	Alternative sequence	1 – 36	36	MSVVG…DRCTP → MGGPPRHGVLDREER in isoform 2.		VSP_007500
Experimental info
	Sequence conflict	175 – 176	2	TA → HS in BAA08446. Ref.2
	Sequence conflict	175 – 176	2	TA → HS in BAA19468. Ref.3
	Sequence conflict	221	1	K → E in BAA08446. Ref.2
	Sequence conflict	221	1	K → E in BAA19468. Ref.3
	Sequence conflict	279	1	A → P in BAA08446. Ref.2
	Sequence conflict	279	1	A → P in BAA19468. Ref.3
	Sequence conflict	308	1	Q → R in BAA08446. Ref.2
	Sequence conflict	308	1	Q → R in BAA19468. Ref.3
	Sequence conflict	483	1	R → S in BAE37350. Ref.4
	Sequence conflict	571	1	T → A in BAE37350. Ref.4
	Sequence conflict	776	1	K → M in AAC52610. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 23, 2003. Version 2. Checksum: B2C5847DA7EAF6B7	FASTA	838	94,382
	10 20 30 40 50 60 MSVVGIDLGF LNCYIAVARS GGIETIANEY SDRCTPACIS LGSRTRAIGN AAKSQIVTNV 70 80 90 100 110 120 RNTIHGFKKL HGRSFDDPIV QTERIRLPYE LQKMPNGSTG VKVRYLEEER PFAIEQVTGM 130 140 150 160 170 180 LLAKLKETSE NALKKPVADC VISIPSFFTD AERRSVMAAA QVAGLNCLRL MNETTAVALA 190 200 210 220 230 240 YGIYKQDLPS LDEKPRNVVF IDMGHSAYQV SVCAFNKGKL KVLATTFDPY LGGRNFDEAL 250 260 270 280 290 300 VDYFCDEFKT KYKINVKENS RALLRLYQEC EKLKKLMSAN ASDLPLNIEC FMNDLDVSSK 310 320 330 340 350 360 MNRAQFEQLC ASLLARVEPP LKSVMDQANL QREDINSIEI VGGATRIPAV KEQVTRFFLK 370 380 390 400 410 420 DISTTLNADE AVARGCALQC AILSPAFKVR EFSITDLVPY SVTLRWKTSF EEGTGECEVF 430 440 450 460 470 480 SKNHPAPFSK VITFHKKEPF ELEAFYTNLH EVPYPDPRIG NFTIQNVFPQ SDGDSSKVKV 490 500 510 520 530 540 KVRINIHGIF SVASASVIEK QNLEGDHNDA AMETEAPKSE GKEDVDKMQV DQEEGGHQKC 550 560 570 580 590 600 HAEHTPEEEI DHTGAKAKAP PSDKQDRINQ TIKKGKIKSI DLPIQSSLYR QLTQDLLNSY 610 620 630 640 650 660 IENEGKMIMQ DKLEKERNDA KNAVEEYVYD FRDKLGTVYE KFITPEDMNK LSAMLEDTEN 670 680 690 700 710 720 WLYEEGEDQP KQVYVDRLQE LKKYGQPIQM KYVEHEERPK ALNDLGKKIQ LVLKVIEAHR 730 740 750 760 770 780 NKDERYDHLD PAEMERVEKY ISDSMNWLNS KMNAQNKLSL TQDPVVKVSE IVTKSKELDN 790 800 810 820 830 FCNPIVYKPK PKVEAPEDKA KTGSEHNGPM DGQSGSETSP DPPKGSSQHT DSGEMEVD
	Isoform 2 (Apg-1b) [UniParc]. Checksum: 38B0704DFEDE5DD4	FASTA	817	92,252
	10 20 30 40 50 60 MGGPPRHGVL DREERACISL GSRTRAIGNA AKSQIVTNVR NTIHGFKKLH GRSFDDPIVQ 70 80 90 100 110 120 TERIRLPYEL QKMPNGSTGV KVRYLEEERP FAIEQVTGML LAKLKETSEN ALKKPVADCV 130 140 150 160 170 180 ISIPSFFTDA ERRSVMAAAQ VAGLNCLRLM NETTAVALAY GIYKQDLPSL DEKPRNVVFI 190 200 210 220 230 240 DMGHSAYQVS VCAFNKGKLK VLATTFDPYL GGRNFDEALV DYFCDEFKTK YKINVKENSR 250 260 270 280 290 300 ALLRLYQECE KLKKLMSANA SDLPLNIECF MNDLDVSSKM NRAQFEQLCA SLLARVEPPL 310 320 330 340 350 360 KSVMDQANLQ REDINSIEIV GGATRIPAVK EQVTRFFLKD ISTTLNADEA VARGCALQCA 370 380 390 400 410 420 ILSPAFKVRE FSITDLVPYS VTLRWKTSFE EGTGECEVFS KNHPAPFSKV ITFHKKEPFE 430 440 450 460 470 480 LEAFYTNLHE VPYPDPRIGN FTIQNVFPQS DGDSSKVKVK VRINIHGIFS VASASVIEKQ 490 500 510 520 530 540 NLEGDHNDAA METEAPKSEG KEDVDKMQVD QEEGGHQKCH AEHTPEEEID HTGAKAKAPP 550 560 570 580 590 600 SDKQDRINQT IKKGKIKSID LPIQSSLYRQ LTQDLLNSYI ENEGKMIMQD KLEKERNDAK 610 620 630 640 650 660 NAVEEYVYDF RDKLGTVYEK FITPEDMNKL SAMLEDTENW LYEEGEDQPK QVYVDRLQEL 670 680 690 700 710 720 KKYGQPIQMK YVEHEERPKA LNDLGKKIQL VLKVIEAHRN KDERYDHLDP AEMERVEKYI 730 740 750 760 770 780 SDSMNWLNSK MNAQNKLSLT QDPVVKVSEI VTKSKELDNF CNPIVYKPKP KVEAPEDKAK 790 800 810 TGSEHNGPMD GQSGSETSPD PPKGSSQHTD SGEMEVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Osmotic stress protein 94 (Osp94). A new member of the Hsp110/SSE gene subfamily." Kojima R., Randall J., Brenner B.M., Gullans S.R. J. Biol. Chem. 271:12327-12332(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"A novel hsp110-related gene, apg-1, that is abundantly expressed in the testis responds to a low temperature heat shock rather than the traditional elevated temperatures." Kaneko Y., Nishiyama H., Nonoguchi K., Higashitsuji H., Kishishita M., Fujita J. J. Biol. Chem. 272:2640-2645(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION. Strain: DDY/STD. Tissue: Testis.
[3]	"Apg-1b, an alternative form of apg-1 transcript." Kaneko Y., Fujita J. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: ddY. Tissue: Testis.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Corpora quadrigemina.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Brain and Salivary gland.
[6]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 135-153; 484-500 AND 622-632, MASS SPECTROMETRY. Tissue: Hippocampus.
[7]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74, MASS SPECTROMETRY. Tissue: Brain cortex.
[8]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-761, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23921 mRNA. Translation: AAC52610.1. D49482 mRNA. Translation: BAA08446.1. AB001926 mRNA. Translation: BAA19468.1. AK033950 mRNA. Translation: BAC28524.1. AK050997 mRNA. Translation: BAC34491.1. AK144225 mRNA. Translation: BAE25783.1. AK163459 mRNA. Translation: BAE37350.1. BC012712 mRNA. Translation: AAH12712.1. BC057002 mRNA. Translation: AAH57002.1. BC110662 mRNA. Translation: AAI10663.1.
IPI	IPI00317710. IPI00317711.
RefSeq	NP_035150.3. NM_011020.3.
UniGene	Mm.39330.
3D structure databases
ProteinModelPortal	P48722.
SMR	P48722. Positions 5-700.
ModBase	Search...
PTM databases
PhosphoSite	P48722.
2D gel databases
REPRODUCTION-2DPAGE	IPI00317710. P48722.
Proteomic databases
PaxDb	P48722.
PRIDE	P48722.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000077083; ENSMUSP00000076336; ENSMUSG00000025757. ENSMUST00000108086; ENSMUSP00000103721; ENSMUSG00000025757.
GeneID	18415.
KEGG	mmu:18415.
UCSC	uc008pbk.1. mouse. uc008pbl.1. mouse.
Organism-specific databases
CTD	22824.
MGI	MGI:107422. Hspa4l.
Phylogenomic databases
eggNOG	COG0443.
GeneTree	ENSGT00390000016919.
HOVERGEN	HBG047955.
InParanoid	P48722.
KO	K09485.
OMA	FITPEDM.
OrthoDB	EOG40K7Z4.
Gene expression databases
ArrayExpress	P48722.
Bgee	P48722.
CleanEx	MM_HSPA4L.
Genevestigator	P48722.
GermOnline	ENSMUSG00000025757. Mus musculus.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00297. HSP70_1. False negative. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HSPA4L. mouse.
NextBio	294048.
SOURCE	Search...

Entry information

Entry name

HS74L_MOUSE

Accession

Primary (citable) accession number: P48722
Secondary accession number(s): P97854 , Q3TQN2, Q3UNG4, Q8BQD0, Q8CC45, Q91X29

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	May 23, 2003
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program