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UniProtKB/Swiss-Prot P48681 (NEST_HUMAN)
Last modified
November 25, 2008.
Version 62.
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Clusters with 100%,
90%,
50% identity |
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Nestin | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1621 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division By similarity. |
| Subunit structure | Interacts with FHOD3 By similarity. |
| Tissue specificity | CNS stem cells. |
| Developmental stage | Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. |
| Sequence similarities | Belongs to the intermediate filament family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Intermediate filament |
| Domain | Coiled coil |
| PTM | Phosphoprotein |
Gene Ontology (GO) | |
| Biological process | central nervous system development Ref.3 Non-traceable author statement. Source: UniProtKB |
| Cellular component | intermediate filament Ref.3 Non-traceable author statement. Source: UniProtKB |
| Molecular function | structural molecule activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1621 | 1621 | Nestin | PRO_0000063853 | |||||
Regions | |||||||||
| Region | 1 – 7 | 7 | Head | ||||||
| Region | 8 – 313 | 306 | Rod | ||||||
| Region | 8 – 43 | 36 | Coil 1A | ||||||
| Region | 44 – 55 | 12 | Linker 1 | ||||||
| Region | 56 – 151 | 96 | Coil 1B | ||||||
| Region | 152 – 173 | 22 | Linker 12 | ||||||
| Region | 174 – 192 | 19 | Coil 2A | ||||||
| Region | 193 – 195 | 3 | Linker 2 | ||||||
| Region | 196 – 313 | 118 | Coil 2B | ||||||
| Region | 314 – 1621 | 1308 | Tail | ||||||
Amino acid modifications | |||||||||
| Modified residue | 311 | 1 | Phosphoserine | ||||||
| Modified residue | 315 | 1 | Phosphothreonine | ||||||
| Modified residue | 320 | 1 | Phosphoserine | ||||||
| Modified residue | 325 | 1 | Phosphoserine | ||||||
| Modified residue | 338 | 1 | Phosphothreonine | ||||||
| Modified residue | 354 | 1 | Phosphothreonine | ||||||
| Modified residue | 384 | 1 | Phosphothreonine | ||||||
| Modified residue | 388 | 1 | Phosphothreonine | ||||||
| Modified residue | 398 | 1 | Phosphoserine | ||||||
| Modified residue | 471 | 1 | Phosphoserine | ||||||
| Modified residue | 548 | 1 | Phosphoserine | ||||||
| Modified residue | 564 | 1 | Phosphoserine | ||||||
| Modified residue | 578 | 1 | Phosphoserine | ||||||
| Modified residue | 588 | 1 | Phosphoserine | ||||||
| Modified residue | 680 | 1 | Phosphoserine | ||||||
| Modified residue | 746 | 1 | Phosphoserine | ||||||
| Modified residue | 768 | 1 | Phosphoserine | ||||||
| Modified residue | 842 | 1 | Phosphoserine | ||||||
| Modified residue | 851 | 1 | Phosphothreonine | ||||||
| Modified residue | 905 | 1 | Phosphoserine | ||||||
| Modified residue | 1286 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1409 | 1 | Phosphoserine | ||||||
| Modified residue | 1418 | 1 | Phosphoserine | ||||||
| Modified residue | 1585 | 1 | Phosphoserine | ||||||
| Modified residue | 1615 | 1 | Phosphoserine | ||||||
| Modified residue | 1617 | 1 | Phosphoserine | ||||||
| Modified residue | 1618 | 1 | Phosphoserine | ||||||
Experimental info | |||||||||
| Sequence conflict | 24 | 1 | A → G in CAA46780. Ref.1 | ||||||
| Sequence conflict | 39 | 1 | E → G in CAA46780. Ref.1 | ||||||
| Sequence conflict | 45 | 1 | A → R in CAA46780. Ref.1 | ||||||
| Sequence conflict | 96 | 1 | Q → E in CAA46780. Ref.1 | ||||||
| Sequence conflict | 130 | 1 | V → G in CAA46780. Ref.1 | ||||||
| Sequence conflict | 161 | 1 | C → L in CAA46780. Ref.1 | ||||||
| Sequence conflict | 167 | 1 | Missing in CAA46780. Ref.1 | ||||||
| Sequence conflict | 205 – 207 | 3 | GQA → DQT in CAA46780. Ref.1 | ||||||
| Sequence conflict | 212 | 1 | G → A in CAA46780. Ref.1 | ||||||
| Sequence conflict | 221 | 1 | G → V in CAA46780. Ref.1 | ||||||
| Sequence conflict | 595 – 612 | 18 | ELLKD…KEAVG → RAIKGCGGSETSRKRGCR in CAA46780. Ref.1 | ||||||
| Sequence conflict | 815 | 1 | V → I in BAE45713. Ref.4 | ||||||
| Sequence conflict | 857 – 908 | 52 | QGAMN…RSPEE → KSGGNESSRKGNSRTTGVCG SEPRDIQTPGRGESGIIEIS GSMEPGEFEISRG in CAA46780. Ref.1 | ||||||
| Sequence conflict | 975 | 1 | E → K in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1008 | 1 | I → F in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1016 | 1 | S → N in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1050 | 1 | A → T in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1101 | 1 | P → L in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1235 | 1 | P → L in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1256 | 1 | Missing in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1275 | 1 | P → L in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1314 – 1325 | 12 | DPTGE…PPPQG → TPLESRGHPLK in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1344 – 1345 | 2 | AP → E in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1472 | 1 | S → N in BAE45713. Ref.4 | ||||||
| Sequence conflict | 1542 | 1 | L → M in BAE45713. Ref.4 | ||||||
| Sequence conflict | 1563 – 1568 | 6 | VGQGMP → SGARNA in CAA46780. Ref.1 | ||||||
| Sequence conflict | 1589 – 1591 | 3 | TSW → RSS in CAA46780. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." Dahlstrand J., McKay R.D.G., Zimmerman L.B., Lendahl U. J. Cell Sci. 103:589-597(1992) [PubMed: 1478958] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta. |
| [2] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene." Yaworsky P.J., Kappen C. Dev. Biol. 205:309-321(1999) [PubMed: 9917366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 297-310. |
| [4] | "Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma." Ohira M., Morohashi A., Nakamura Y., Isogai E., Furuya K., Hamano S., Machida T., Aoyama M., Fukumura M., Miyazaki K., Suzuki Y., Sugano S., Hirato J., Nakagawara A. Cancer Lett. 197:63-68(2003) [PubMed: 12880961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 604-1621. Tissue: Neuroblastoma. |
| [5] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-354; SER-680; SER-768; SER-1409; SER-1418; SER-1617 AND SER-1618, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-311; THR-315; SER-320; SER-325; THR-338; THR-384; THR-388; SER-398; SER-471; SER-548; SER-564; SER-578; SER-588; SER-746; SER-768; SER-842; THR-851; SER-905; SER-1418; SER-1585; SER-1615; SER-1617 AND SER-1618, MASS SPECTROMETRY. |
| [7] | "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1418, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |

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