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Protein

Nestin

Gene

NES

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • brain development Source: UniProtKB
  • cell projection morphogenesis Source: Ensembl
  • central nervous system development Source: UniProtKB
  • embryonic camera-type eye development Source: UniProtKB
  • G2/M transition of mitotic cell cycle Source: DFLAT
  • negative regulation of catalytic activity Source: UniProtKB
  • negative regulation of neuron apoptotic process Source: Ensembl
  • negative regulation of protein binding Source: UniProtKB
  • positive regulation of intermediate filament depolymerization Source: UniProtKB
  • positive regulation of neural precursor cell proliferation Source: UniProtKB
  • stem cell proliferation Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Neurogenesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132688-MONOMER.
SIGNORiP48681.

Names & Taxonomyi

Protein namesi
Recommended name:
Nestin
Gene namesi
Name:NES
ORF Names:Nbla00170
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:7756. NES.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: DFLAT
  • extracellular matrix Source: BHF-UCL
  • intermediate filament Source: UniProtKB
  • intermediate filament cytoskeleton Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

Pathology & Biotechi

Organism-specific databases

DisGeNETi10763.
OpenTargetsiENSG00000132688.
PharmGKBiPA31556.

Polymorphism and mutation databases

BioMutaiNES.
DMDMi146345464.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638531 – 1621NestinAdd BLAST1621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei311PhosphoserineCombined sources1
Modified residuei315PhosphothreonineCombined sources1
Modified residuei325PhosphoserineCombined sources1
Modified residuei338PhosphothreonineCombined sources1
Modified residuei355PhosphoserineCombined sources1
Modified residuei358PhosphoserineCombined sources1
Modified residuei388PhosphothreonineCombined sources1
Modified residuei398PhosphoserineCombined sources1
Modified residuei471PhosphoserineCombined sources1
Modified residuei476PhosphoserineCombined sources1
Modified residuei548PhosphoserineCombined sources1
Modified residuei564PhosphoserineCombined sources1
Modified residuei578PhosphoserineCombined sources1
Modified residuei588PhosphoserineCombined sources1
Modified residuei638PhosphoserineBy similarity1
Modified residuei680PhosphoserineCombined sources1
Modified residuei702PhosphoserineBy similarity1
Modified residuei746PhosphoserineCombined sources1
Modified residuei768PhosphoserineCombined sources1
Modified residuei790PhosphoserineCombined sources1
Cross-linki811Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Modified residuei820PhosphoserineCombined sources1
Modified residuei831PhosphoserineCombined sources1
Modified residuei851PhosphothreonineCombined sources1
Modified residuei894PhosphoserineCombined sources1
Modified residuei905PhosphoserineCombined sources1
Modified residuei913PhosphoserineCombined sources1
Modified residuei934PhosphoserineCombined sources1
Modified residuei1016PhosphoserineCombined sources1
Modified residuei1261PhosphoserineCombined sources1
Modified residuei1282PhosphoserineCombined sources1
Modified residuei1286PhosphoserineCombined sources1
Modified residuei1310PhosphoserineBy similarity1
Modified residuei1347PhosphoserineCombined sources1
Modified residuei1409PhosphoserineCombined sources1
Modified residuei1418PhosphoserineCombined sources1
Modified residuei1452PhosphoserineCombined sources1
Modified residuei1496PhosphoserineCombined sources1
Modified residuei1498PhosphoserineCombined sources1
Modified residuei1577PhosphoserineCombined sources1
Modified residuei1617PhosphoserineCombined sources1
Modified residuei1618PhosphoserineCombined sources1

Post-translational modificationi

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP48681.
MaxQBiP48681.
PaxDbiP48681.
PeptideAtlasiP48681.
PRIDEiP48681.

PTM databases

iPTMnetiP48681.
PhosphoSitePlusiP48681.

Expressioni

Tissue specificityi

CNS stem cells.

Developmental stagei

Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

Gene expression databases

BgeeiENSG00000132688.
CleanExiHS_NES.
GenevisibleiP48681. HS.

Organism-specific databases

HPAiCAB005889.
CAB058692.
HPA006286.
HPA007007.
HPA026111.

Interactioni

Subunit structurei

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115983. 65 interactors.
IntActiP48681. 46 interactors.
STRINGi9606.ENSP00000357206.

Structurei

3D structure databases

ProteinModelPortaliP48681.
SMRiP48681.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 7Head7
Regioni8 – 313RodAdd BLAST306
Regioni8 – 43Coil 1AAdd BLAST36
Regioni44 – 55Linker 1Add BLAST12
Regioni56 – 151Coil 1BAdd BLAST96
Regioni152 – 173Linker 12Add BLAST22
Regioni174 – 192Coil 2AAdd BLAST19
Regioni193 – 195Linker 23
Regioni196 – 313Coil 2BAdd BLAST118
Regioni314 – 1621TailAdd BLAST1308

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IRNV. Eukaryota.
ENOG410XZH4. LUCA.
GeneTreeiENSGT00730000110953.
HOGENOMiHOG000113766.
HOVERGENiHBG006463.
InParanoidiP48681.
KOiK07609.
OMAiQSPEDHA.
OrthoDBiEOG091G01X9.
PhylomeDBiP48681.
TreeFamiTF336633.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR031211. Nestin.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 2 hits.
PTHR23239:SF131. PTHR23239:SF131. 2 hits.
PfamiPF00038. Filament. 2 hits.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGCMGEESF QMWELNRRLE AYLARVKALE EQNELLSAEL GGLRAQSADT
60 70 80 90 100
SWRAHADDEL AALRALVDQR WREKHAAEVA RDNLAEELEG VAGRCQQLRL
110 120 130 140 150
ARERTTEEVA RNRRAVEAEK CARAWLSSQV AELERELEAL RVAHEEERVG
160 170 180 190 200
LNAQAACAPR CPAPPRGPPA PAPEVEELAR RLGEAWRGAV RGYQERVAHM
210 220 230 240 250
ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA ALEQRLEGRW
260 270 280 290 300
QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA
310 320 330 340 350
TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV
360 370 380 390 400
LSPTSLPSPL PATLETPVPA FLKNQEFLQA RTPTLASTPI PPTPQAPSPA
410 420 430 440 450
VDAEIRAQDA PLSLLQTQGG RKQAPEPLRA EARVAIPASV LPGPEEPGGQ
460 470 480 490 500
RQEASTGQSP EDHASLAPPL SPDHSSLEAK DGESGGSRVF SICRGEGEGQ
510 520 530 540 550
IWGLVEKETA IEGKVVSSLQ QEIWEEEDLN RKEIQDSQVP LEKETLKSLG
560 570 580 590 600
EEIQESLKTL ENQSHETLER ENQECPRSLE EDLETLKSLE KENKELLKDV
610 620 630 640 650
EVVRPLEKEA VGQLKPTGKE DTQTLQSLQK ENQELMKSLE GNLETFLFPG
660 670 680 690 700
TENQELVSSL QENLESLTAL EKENQEPLRS PEVGDEEALR PLTKENQEPL
710 720 730 740 750
RSLEDENKEA FRSLEKENQE PLKTLEEEDQ SIVRPLETEN HKSLRSLEEQ
760 770 780 790 800
DQETLRTLEK ETQQRRRSLG EQDQMTLRPP EKVDLEPLKS LDQEIARPLE
810 820 830 840 850
NENQEFLKSL KEESVEAVKS LETEILESLK SAGQENLETL KSPETQAPLW
860 870 880 890 900
TPEEINQGAM NPLEKEIQEP LESVEVNQET FRLLEEENQE SLRSLGAWNL
910 920 930 940 950
ENLRSPEEVD KESQRNLEEE ENLGKGEYQE SLRSLEEEGQ ELPQSADVQR
960 970 980 990 1000
WEDTVEKDQE LAQESPPGMA GVENEDEAEL NLREQDGFTG KEEVVEQGEL
1010 1020 1030 1040 1050
NATEEVWIPG EGHPESPEPK EQRGLVEGAS VKGGAEGLQD PEGQSQQVGA
1060 1070 1080 1090 1100
PGLQAPQGLP EAIEPLVEDD VAPGGDQASP EVMLGSEPAM GESAAGAEPG
1110 1120 1130 1140 1150
PGQGVGGLGD PGHLTREEVM EPPLEEESLE AKRVQGLEGP RKDLEEAGGL
1160 1170 1180 1190 1200
GTEFSELPGK SRDPWEPPRE GREESEAEAP RGAEEAFPAE TLGHTGSDAP
1210 1220 1230 1240 1250
SPWPLGSEEA EEDVPPVLVS PSPTYTPILE DAPGPQPQAE GSQEASWGVQ
1260 1270 1280 1290 1300
GRAEALGKVE SEQEELGSGE IPEGPQEEGE ESREESEEDE LGETLPDSTP
1310 1320 1330 1340 1350
LGFYLRSPTS PRWDPTGEQR PPPQGETGKE GWDPAVLASE GLEAPPSEKE
1360 1370 1380 1390 1400
EGEEGEEECG RDSDLSEEFE DLGTEAPFLP GVPGEVAEPL GQVPQLLLDP
1410 1420 1430 1440 1450
AAWDRDGESD GFADEEESGE EGEEDQEEGR EPGAGRWGPG SSVGSLQALS
1460 1470 1480 1490 1500
SSQRGEFLES DSVSVSVPWD DSLRGAVAGA PKTALETESQ DSAEPSGSEE
1510 1520 1530 1540 1550
ESDPVSLERE DKVPGPLEIP SGMEDAGPGA DIIGVNGQGP NLEGKSQHVN
1560 1570 1580 1590 1600
GGVMNGLEQS EEVGQGMPLV SEGDRGSPFQ EEEGSALKTS WAGAPVHLGQ
1610 1620
GQFLKFTQRE GDRESWSSGE D
Length:1,621
Mass (Da):177,439
Last modified:May 1, 2007 - v2
Checksum:i0E4F967C11F44C13
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24A → G in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti39E → G in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti45A → R in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti96Q → E in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti130V → G in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti161C → L in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti167Missing in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti205 – 207GQA → DQT in CAA46780 (PubMed:1478958).Curated3
Sequence conflicti212G → A in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti221G → V in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti595 – 612ELLKD…KEAVG → RAIKGCGGSETSRKRGCR in CAA46780 (PubMed:1478958).CuratedAdd BLAST18
Sequence conflicti857 – 908QGAMN…RSPEE → KSGGNESSRKGNSRTTGVCG SEPRDIQTPGRGESGIIEIS GSMEPGEFEISRG in CAA46780 (PubMed:1478958).CuratedAdd BLAST52
Sequence conflicti975E → K in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti1008I → F in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti1050A → T in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti1235P → L in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti1256Missing in CAA46780 (PubMed:1478958).Curated1
Sequence conflicti1314 – 1325DPTGE…PPPQG → TPLESRGHPLK in CAA46780 (PubMed:1478958).CuratedAdd BLAST12
Sequence conflicti1344 – 1345AP → E in CAA46780 (PubMed:1478958).Curated2
Sequence conflicti1472S → N in BAE45713 (PubMed:12880961).Curated1
Sequence conflicti1542L → M in BAE45713 (PubMed:12880961).Curated1
Sequence conflicti1563 – 1568VGQGMP → SGARNA in CAA46780 (PubMed:1478958).Curated6
Sequence conflicti1589 – 1591TSW → RSS in CAA46780 (PubMed:1478958).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061301130V → A.Corresponds to variant rs4278369dbSNPEnsembl.1
Natural variantiVAR_049814815V → I.1 PublicationCorresponds to variant rs951781dbSNPEnsembl.1
Natural variantiVAR_0498151016S → N.1 PublicationCorresponds to variant rs2365718dbSNPEnsembl.1
Natural variantiVAR_0498161101P → L.1 PublicationCorresponds to variant rs2886443dbSNPEnsembl.1
Natural variantiVAR_0498171133R → S.Corresponds to variant rs17393797dbSNPEnsembl.1
Natural variantiVAR_0498181275P → L.Combined sources1 PublicationCorresponds to variant rs3748570dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590666 Genomic DNA. Translation: CAI16338.1.
X65964 Genomic DNA. Translation: CAA46780.1.
AF004335 Genomic DNA. Translation: AAB64426.1.
AB073350 mRNA. Translation: BAE45713.1.
CCDSiCCDS1151.1.
PIRiS21424.
RefSeqiNP_006608.1. NM_006617.1.
UniGeneiHs.527971.

Genome annotation databases

EnsembliENST00000368223; ENSP00000357206; ENSG00000132688.
GeneIDi10763.
KEGGihsa:10763.
UCSCiuc001fpq.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590666 Genomic DNA. Translation: CAI16338.1.
X65964 Genomic DNA. Translation: CAA46780.1.
AF004335 Genomic DNA. Translation: AAB64426.1.
AB073350 mRNA. Translation: BAE45713.1.
CCDSiCCDS1151.1.
PIRiS21424.
RefSeqiNP_006608.1. NM_006617.1.
UniGeneiHs.527971.

3D structure databases

ProteinModelPortaliP48681.
SMRiP48681.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115983. 65 interactors.
IntActiP48681. 46 interactors.
STRINGi9606.ENSP00000357206.

PTM databases

iPTMnetiP48681.
PhosphoSitePlusiP48681.

Polymorphism and mutation databases

BioMutaiNES.
DMDMi146345464.

Proteomic databases

EPDiP48681.
MaxQBiP48681.
PaxDbiP48681.
PeptideAtlasiP48681.
PRIDEiP48681.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368223; ENSP00000357206; ENSG00000132688.
GeneIDi10763.
KEGGihsa:10763.
UCSCiuc001fpq.4. human.

Organism-specific databases

CTDi10763.
DisGeNETi10763.
GeneCardsiNES.
H-InvDBHIX0001169.
HGNCiHGNC:7756. NES.
HPAiCAB005889.
CAB058692.
HPA006286.
HPA007007.
HPA026111.
MIMi600915. gene.
neXtProtiNX_P48681.
OpenTargetsiENSG00000132688.
PharmGKBiPA31556.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IRNV. Eukaryota.
ENOG410XZH4. LUCA.
GeneTreeiENSGT00730000110953.
HOGENOMiHOG000113766.
HOVERGENiHBG006463.
InParanoidiP48681.
KOiK07609.
OMAiQSPEDHA.
OrthoDBiEOG091G01X9.
PhylomeDBiP48681.
TreeFamiTF336633.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132688-MONOMER.
SIGNORiP48681.

Miscellaneous databases

ChiTaRSiNES. human.
GeneWikiiNestin_(protein).
GenomeRNAii10763.
PROiP48681.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132688.
CleanExiHS_NES.
GenevisibleiP48681. HS.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR031211. Nestin.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 2 hits.
PTHR23239:SF131. PTHR23239:SF131. 2 hits.
PfamiPF00038. Filament. 2 hits.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEST_HUMAN
AccessioniPrimary (citable) accession number: P48681
Secondary accession number(s): O00552, Q3LIF5, Q5SYZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.