Reviewed, UniProtKB/Swiss-Prot P48681 (NEST_HUMAN)

Names and origin

Protein names

Recommended name:
    Nestin

Gene names

Name:	NES
ORF Names:	Nbla00170

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1621 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division By similarity.
Subunit structure	Interacts with FHOD3 By similarity.
Tissue specificity	CNS stem cells.
Developmental stage	Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
Sequence similarities	Belongs to the intermediate filament family.

Ontologies

Keywords
Cellular component	Intermediate filament
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	central nervous system development Ref.3 Non-traceable author statement. Source: UniProtKB
Cellular component	intermediate filament Ref.3 Non-traceable author statement. Source: UniProtKB
Molecular function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1621	1621	Nestin		PRO_0000063853
Regions
	Region	1 – 7	7	Head
	Region	8 – 313	306	Rod
	Region	8 – 43	36	Coil 1A
	Region	44 – 55	12	Linker 1
	Region	56 – 151	96	Coil 1B
	Region	152 – 173	22	Linker 12
	Region	174 – 192	19	Coil 2A
	Region	193 – 195	3	Linker 2
	Region	196 – 313	118	Coil 2B
	Region	314 – 1621	1308	Tail
Amino acid modifications
	Modified residue	311	1	Phosphoserine Ref.6
	Modified residue	315	1	Phosphothreonine Ref.6
	Modified residue	320	1	Phosphoserine Ref.6
	Modified residue	325	1	Phosphoserine Ref.6
	Modified residue	338	1	Phosphothreonine Ref.6
	Modified residue	354	1	Phosphothreonine Ref.5
	Modified residue	384	1	Phosphothreonine Ref.6
	Modified residue	388	1	Phosphothreonine Ref.6
	Modified residue	398	1	Phosphoserine Ref.6
	Modified residue	471	1	Phosphoserine Ref.6
	Modified residue	548	1	Phosphoserine Ref.6
	Modified residue	564	1	Phosphoserine Ref.6
	Modified residue	578	1	Phosphoserine Ref.6
	Modified residue	588	1	Phosphoserine Ref.6
	Modified residue	680	1	Phosphoserine Ref.5
	Modified residue	746	1	Phosphoserine Ref.6
	Modified residue	768	1	Phosphoserine Ref.6 Ref.5
	Modified residue	842	1	Phosphoserine Ref.6
	Modified residue	851	1	Phosphothreonine Ref.6
	Modified residue	905	1	Phosphoserine Ref.6
	Modified residue	1286	1	Phosphoserine By similarity
	Modified residue	1347	1	Phosphoserine Ref.8
	Modified residue	1409	1	Phosphoserine Ref.5
	Modified residue	1418	1	Phosphoserine Ref.6 Ref.5 Ref.7
	Modified residue	1585	1	Phosphoserine Ref.6
	Modified residue	1615	1	Phosphoserine Ref.6
	Modified residue	1617	1	Phosphoserine Ref.6 Ref.5
	Modified residue	1618	1	Phosphoserine Ref.6 Ref.5
Natural variations
	Natural variant	130	1	V → A: dbSNP rs4278369.		VAR_061301
	Natural variant	815	1	V → I: dbSNP rs951781. Ref.4		VAR_049814
	Natural variant	1016	1	S → N: dbSNP rs2365718. Ref.1		VAR_049815
	Natural variant	1101	1	P → L: dbSNP rs2886443. Ref.1		VAR_049816
	Natural variant	1133	1	R → S: dbSNP rs17393797.		VAR_049817
	Natural variant	1275	1	P → L: dbSNP rs3748570. Ref.1		VAR_049818
Experimental info
	Sequence conflict	24	1	A → G in CAA46780. Ref.1
	Sequence conflict	39	1	E → G in CAA46780. Ref.1
	Sequence conflict	45	1	A → R in CAA46780. Ref.1
	Sequence conflict	96	1	Q → E in CAA46780. Ref.1
	Sequence conflict	130	1	V → G in CAA46780. Ref.1
	Sequence conflict	161	1	C → L in CAA46780. Ref.1
	Sequence conflict	167	1	Missing in CAA46780. Ref.1
	Sequence conflict	205 – 207	3	GQA → DQT in CAA46780. Ref.1
	Sequence conflict	212	1	G → A in CAA46780. Ref.1
	Sequence conflict	221	1	G → V in CAA46780. Ref.1
	Sequence conflict	595 – 612	18	ELLKD…KEAVG → RAIKGCGGSETSRKRGCR in CAA46780. Ref.1
	Sequence conflict	857 – 908	52	QGAMN…RSPEE → KSGGNESSRKGNSRTTGVCG SEPRDIQTPGRGESGIIEIS GSMEPGEFEISRG in CAA46780. Ref.1
	Sequence conflict	975	1	E → K in CAA46780. Ref.1
	Sequence conflict	1008	1	I → F in CAA46780. Ref.1
	Sequence conflict	1050	1	A → T in CAA46780. Ref.1
	Sequence conflict	1235	1	P → L in CAA46780. Ref.1
	Sequence conflict	1256	1	Missing in CAA46780. Ref.1
	Sequence conflict	1314 – 1325	12	DPTGE…PPPQG → TPLESRGHPLK in CAA46780. Ref.1
	Sequence conflict	1344 – 1345	2	AP → E in CAA46780. Ref.1
	Sequence conflict	1472	1	S → N in BAE45713. Ref.4
	Sequence conflict	1542	1	L → M in BAE45713. Ref.4
	Sequence conflict	1563 – 1568	6	VGQGMP → SGARNA in CAA46780. Ref.1
	Sequence conflict	1589 – 1591	3	TSW → RSS in CAA46780. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48681-1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: 0E4F967C11F44C13	FASTA	1,621	177,439
	10 20 30 40 50 60 MEGCMGEESF QMWELNRRLE AYLARVKALE EQNELLSAEL GGLRAQSADT SWRAHADDEL 70 80 90 100 110 120 AALRALVDQR WREKHAAEVA RDNLAEELEG VAGRCQQLRL ARERTTEEVA RNRRAVEAEK 130 140 150 160 170 180 CARAWLSSQV AELERELEAL RVAHEEERVG LNAQAACAPR CPAPPRGPPA PAPEVEELAR 190 200 210 220 230 240 RLGEAWRGAV RGYQERVAHM ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA 250 260 270 280 290 300 ALEQRLEGRW QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA 310 320 330 340 350 360 TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV LSPTSLPSPL 370 380 390 400 410 420 PATLETPVPA FLKNQEFLQA RTPTLASTPI PPTPQAPSPA VDAEIRAQDA PLSLLQTQGG 430 440 450 460 470 480 RKQAPEPLRA EARVAIPASV LPGPEEPGGQ RQEASTGQSP EDHASLAPPL SPDHSSLEAK 490 500 510 520 530 540 DGESGGSRVF SICRGEGEGQ IWGLVEKETA IEGKVVSSLQ QEIWEEEDLN RKEIQDSQVP 550 560 570 580 590 600 LEKETLKSLG EEIQESLKTL ENQSHETLER ENQECPRSLE EDLETLKSLE KENKELLKDV 610 620 630 640 650 660 EVVRPLEKEA VGQLKPTGKE DTQTLQSLQK ENQELMKSLE GNLETFLFPG TENQELVSSL 670 680 690 700 710 720 QENLESLTAL EKENQEPLRS PEVGDEEALR PLTKENQEPL RSLEDENKEA FRSLEKENQE 730 740 750 760 770 780 PLKTLEEEDQ SIVRPLETEN HKSLRSLEEQ DQETLRTLEK ETQQRRRSLG EQDQMTLRPP 790 800 810 820 830 840 EKVDLEPLKS LDQEIARPLE NENQEFLKSL KEESVEAVKS LETEILESLK SAGQENLETL 850 860 870 880 890 900 KSPETQAPLW TPEEINQGAM NPLEKEIQEP LESVEVNQET FRLLEEENQE SLRSLGAWNL 910 920 930 940 950 960 ENLRSPEEVD KESQRNLEEE ENLGKGEYQE SLRSLEEEGQ ELPQSADVQR WEDTVEKDQE 970 980 990 1000 1010 1020 LAQESPPGMA GVENEDEAEL NLREQDGFTG KEEVVEQGEL NATEEVWIPG EGHPESPEPK 1030 1040 1050 1060 1070 1080 EQRGLVEGAS VKGGAEGLQD PEGQSQQVGA PGLQAPQGLP EAIEPLVEDD VAPGGDQASP 1090 1100 1110 1120 1130 1140 EVMLGSEPAM GESAAGAEPG PGQGVGGLGD PGHLTREEVM EPPLEEESLE AKRVQGLEGP 1150 1160 1170 1180 1190 1200 RKDLEEAGGL GTEFSELPGK SRDPWEPPRE GREESEAEAP RGAEEAFPAE TLGHTGSDAP 1210 1220 1230 1240 1250 1260 SPWPLGSEEA EEDVPPVLVS PSPTYTPILE DAPGPQPQAE GSQEASWGVQ GRAEALGKVE 1270 1280 1290 1300 1310 1320 SEQEELGSGE IPEGPQEEGE ESREESEEDE LGETLPDSTP LGFYLRSPTS PRWDPTGEQR 1330 1340 1350 1360 1370 1380 PPPQGETGKE GWDPAVLASE GLEAPPSEKE EGEEGEEECG RDSDLSEEFE DLGTEAPFLP 1390 1400 1410 1420 1430 1440 GVPGEVAEPL GQVPQLLLDP AAWDRDGESD GFADEEESGE EGEEDQEEGR EPGAGRWGPG 1450 1460 1470 1480 1490 1500 SSVGSLQALS SSQRGEFLES DSVSVSVPWD DSLRGAVAGA PKTALETESQ DSAEPSGSEE 1510 1520 1530 1540 1550 1560 ESDPVSLERE DKVPGPLEIP SGMEDAGPGA DIIGVNGQGP NLEGKSQHVN GGVMNGLEQS 1570 1580 1590 1600 1610 1620 EEVGQGMPLV SEGDRGSPFQ EEEGSALKTS WAGAPVHLGQ GQFLKFTQRE GDRESWSSGE D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." Dahlstrand J., McKay R.D.G., Zimmerman L.B., Lendahl U. J. Cell Sci. 103:589-597(1992) [PubMed: 1478958] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-1016; LEU-1101 AND LEU-1275. Tissue: Placenta.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene." Yaworsky P.J., Kappen C. Dev. Biol. 205:309-321(1999) [PubMed: 9917366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 297-310.
[4]	"Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma." Ohira M., Morohashi A., Nakamura Y., Isogai E., Furuya K., Hamano S., Machida T., Aoyama M., Fukumura M., Miyazaki K., Suzuki Y., Sugano S., Hirato J., Nakagawara A. Cancer Lett. 197:63-68(2003) [PubMed: 12880961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 604-1621, VARIANT ILE-815. Tissue: Neuroblastoma.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-354; SER-680; SER-768; SER-1409; SER-1418; SER-1617 AND SER-1618, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-311; THR-315; SER-320; SER-325; THR-338; THR-384; THR-388; SER-398; SER-471; SER-548; SER-564; SER-578; SER-588; SER-746; SER-768; SER-842; THR-851; SER-905; SER-1418; SER-1585; SER-1615; SER-1617 AND SER-1618, MASS SPECTROMETRY.
[7]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1418, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1347, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590666 Genomic DNA. Translation: CAI16338.1. X65964 Genomic DNA. Translation: CAA46780.1. AF004335 Genomic DNA. Translation: AAB64426.1. AB073350 mRNA. Translation: BAE45713.1.
IPI	IPI00010800.
PIR	S21424.
RefSeq	NP_006608.1.
UniGene	Hs.527971
3D structure databases
SMR	P48681. Positions 240-312.
ModBase	Search...
Protein-protein interaction databases
STRING	P48681.
PTM databases
PhosphoSite	P48681.
Proteomic databases
PRIDE	P48681.
Genome annotation databases
Ensembl	ENST00000368223; ENSP00000357206; ENSG00000132688; Homo sapiens. [Genome view]
GeneID	10763.
KEGG	hsa:10763.
UCSC	uc001fpq.1. human.
Organism-specific databases
CTD	10763.
GeneCards	GC01M154905.
HGNC	HGNC:7756. NES.
HPA	CAB005889. HPA007007. HPA026111.
MIM	600915. gene.
PharmGKB	PA31556.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12746.
HOGENOM	HBG126246.
HOVERGEN	P48681.
InParanoid	P48681.
OMA	WDRDGES.
OrthoDB	EOG947JDS.
PhylomeDB	P48681.
Gene expression databases
ArrayExpress	P48681.
Bgee	P48681.
CleanEx	HS_NES.
Genevestigator	P48681.
GermOnline	ENSG00000132688. Homo sapiens.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. IPR018039. Intermediate_filament_CS. [Graphical view]
PANTHER	PTHR23239. IF. 1 hit.
Pfam	PF00038. Filament. 2 hits. [Graphical view]
PROSITE	PS00226. IF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	40873.
SOURCE	Search...

Entry information

Entry name

NEST_HUMAN

Accession

Primary (citable) accession number: P48681
Secondary accession number(s): O00552, Q3LIF5, Q5SYZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	May 1, 2007
Last modified:	January 19, 2010
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.