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Reviewed, UniProtKB/Swiss-Prot P48681 (NEST_HUMAN)

Last modified November 25, 2008. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Nestin
Gene names
Name: NES
ORF Names: Nbla00170
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length1621 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division By similarity.

Subunit structure

Interacts with FHOD3 By similarity.

Tissue specificity

CNS stem cells.

Developmental stage

Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

Sequence similarities

Belongs to the intermediate filament family.

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Ontologies

Keywords

   Cellular componentIntermediate filament
   DomainCoiled coil
   PTMPhosphoprotein

Gene Ontology (GO)

   Biological processcentral nervous system development Ref.3

Non-traceable author statement. Source: UniProtKB

   Cellular componentintermediate filament Ref.3

Non-traceable author statement. Source: UniProtKB

   Molecular functionstructural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16211621Nestin
PRO_0000063853

Regions

Region1 – 77Head
Region8 – 313306Rod
Region8 – 4336Coil 1A
Region44 – 5512Linker 1
Region56 – 15196Coil 1B
Region152 – 17322Linker 12
Region174 – 19219Coil 2A
Region193 – 1953Linker 2
Region196 – 313118Coil 2B
Region314 – 16211308Tail

Amino acid modifications

Modified residue3111Phosphoserine
Modified residue3151Phosphothreonine
Modified residue3201Phosphoserine
Modified residue3251Phosphoserine
Modified residue3381Phosphothreonine
Modified residue3541Phosphothreonine
Modified residue3841Phosphothreonine
Modified residue3881Phosphothreonine
Modified residue3981Phosphoserine
Modified residue4711Phosphoserine
Modified residue5481Phosphoserine
Modified residue5641Phosphoserine
Modified residue5781Phosphoserine
Modified residue5881Phosphoserine
Modified residue6801Phosphoserine
Modified residue7461Phosphoserine
Modified residue7681Phosphoserine
Modified residue8421Phosphoserine
Modified residue8511Phosphothreonine
Modified residue9051Phosphoserine
Modified residue12861Phosphoserine By similarity
Modified residue14091Phosphoserine
Modified residue14181Phosphoserine
Modified residue15851Phosphoserine
Modified residue16151Phosphoserine
Modified residue16171Phosphoserine
Modified residue16181Phosphoserine

Experimental info

Sequence conflict241A → G in CAA46780. Ref.1
Sequence conflict391E → G in CAA46780. Ref.1
Sequence conflict451A → R in CAA46780. Ref.1
Sequence conflict961Q → E in CAA46780. Ref.1
Sequence conflict1301V → G in CAA46780. Ref.1
Sequence conflict1611C → L in CAA46780. Ref.1
Sequence conflict1671Missing in CAA46780. Ref.1
Sequence conflict205 – 2073GQA → DQT in CAA46780. Ref.1
Sequence conflict2121G → A in CAA46780. Ref.1
Sequence conflict2211G → V in CAA46780. Ref.1
Sequence conflict595 – 61218ELLKD…KEAVG → RAIKGCGGSETSRKRGCR in CAA46780. Ref.1
Sequence conflict8151V → I in BAE45713. Ref.4
Sequence conflict857 – 90852QGAMN…RSPEE → KSGGNESSRKGNSRTTGVCG SEPRDIQTPGRGESGIIEIS GSMEPGEFEISRG in CAA46780. Ref.1
Sequence conflict9751E → K in CAA46780. Ref.1
Sequence conflict10081I → F in CAA46780. Ref.1
Sequence conflict10161S → N in CAA46780. Ref.1
Sequence conflict10501A → T in CAA46780. Ref.1
Sequence conflict11011P → L in CAA46780. Ref.1
Sequence conflict12351P → L in CAA46780. Ref.1
Sequence conflict12561Missing in CAA46780. Ref.1
Sequence conflict12751P → L in CAA46780. Ref.1
Sequence conflict1314 – 132512DPTGE…PPPQG → TPLESRGHPLK in CAA46780. Ref.1
Sequence conflict1344 – 13452AP → E in CAA46780. Ref.1
Sequence conflict14721S → N in BAE45713. Ref.4
Sequence conflict15421L → M in BAE45713. Ref.4
Sequence conflict1563 – 15686VGQGMP → SGARNA in CAA46780. Ref.1
Sequence conflict1589 – 15913TSW → RSS in CAA46780. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P48681-1 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 0E4F967C11F44C13

FASTA1,621177,439
        10         20         30         40         50         60 
MEGCMGEESF QMWELNRRLE AYLARVKALE EQNELLSAEL GGLRAQSADT SWRAHADDEL 

        70         80         90        100        110        120 
AALRALVDQR WREKHAAEVA RDNLAEELEG VAGRCQQLRL ARERTTEEVA RNRRAVEAEK 

       130        140        150        160        170        180 
CARAWLSSQV AELERELEAL RVAHEEERVG LNAQAACAPR CPAPPRGPPA PAPEVEELAR 

       190        200        210        220        230        240 
RLGEAWRGAV RGYQERVAHM ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA 

       250        260        270        280        290        300 
ALEQRLEGRW QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA 

       310        320        330        340        350        360 
TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV LSPTSLPSPL 

       370        380        390        400        410        420 
PATLETPVPA FLKNQEFLQA RTPTLASTPI PPTPQAPSPA VDAEIRAQDA PLSLLQTQGG 

       430        440        450        460        470        480 
RKQAPEPLRA EARVAIPASV LPGPEEPGGQ RQEASTGQSP EDHASLAPPL SPDHSSLEAK 

       490        500        510        520        530        540 
DGESGGSRVF SICRGEGEGQ IWGLVEKETA IEGKVVSSLQ QEIWEEEDLN RKEIQDSQVP 

       550        560        570        580        590        600 
LEKETLKSLG EEIQESLKTL ENQSHETLER ENQECPRSLE EDLETLKSLE KENKELLKDV 

       610        620        630        640        650        660 
EVVRPLEKEA VGQLKPTGKE DTQTLQSLQK ENQELMKSLE GNLETFLFPG TENQELVSSL 

       670        680        690        700        710        720 
QENLESLTAL EKENQEPLRS PEVGDEEALR PLTKENQEPL RSLEDENKEA FRSLEKENQE 

       730        740        750        760        770        780 
PLKTLEEEDQ SIVRPLETEN HKSLRSLEEQ DQETLRTLEK ETQQRRRSLG EQDQMTLRPP 

       790        800        810        820        830        840 
EKVDLEPLKS LDQEIARPLE NENQEFLKSL KEESVEAVKS LETEILESLK SAGQENLETL 

       850        860        870        880        890        900 
KSPETQAPLW TPEEINQGAM NPLEKEIQEP LESVEVNQET FRLLEEENQE SLRSLGAWNL 

       910        920        930        940        950        960 
ENLRSPEEVD KESQRNLEEE ENLGKGEYQE SLRSLEEEGQ ELPQSADVQR WEDTVEKDQE 

       970        980        990       1000       1010       1020 
LAQESPPGMA GVENEDEAEL NLREQDGFTG KEEVVEQGEL NATEEVWIPG EGHPESPEPK 

      1030       1040       1050       1060       1070       1080 
EQRGLVEGAS VKGGAEGLQD PEGQSQQVGA PGLQAPQGLP EAIEPLVEDD VAPGGDQASP 

      1090       1100       1110       1120       1130       1140 
EVMLGSEPAM GESAAGAEPG PGQGVGGLGD PGHLTREEVM EPPLEEESLE AKRVQGLEGP 

      1150       1160       1170       1180       1190       1200 
RKDLEEAGGL GTEFSELPGK SRDPWEPPRE GREESEAEAP RGAEEAFPAE TLGHTGSDAP 

      1210       1220       1230       1240       1250       1260 
SPWPLGSEEA EEDVPPVLVS PSPTYTPILE DAPGPQPQAE GSQEASWGVQ GRAEALGKVE 

      1270       1280       1290       1300       1310       1320 
SEQEELGSGE IPEGPQEEGE ESREESEEDE LGETLPDSTP LGFYLRSPTS PRWDPTGEQR 

      1330       1340       1350       1360       1370       1380 
PPPQGETGKE GWDPAVLASE GLEAPPSEKE EGEEGEEECG RDSDLSEEFE DLGTEAPFLP 

      1390       1400       1410       1420       1430       1440 
GVPGEVAEPL GQVPQLLLDP AAWDRDGESD GFADEEESGE EGEEDQEEGR EPGAGRWGPG 

      1450       1460       1470       1480       1490       1500 
SSVGSLQALS SSQRGEFLES DSVSVSVPWD DSLRGAVAGA PKTALETESQ DSAEPSGSEE 

      1510       1520       1530       1540       1550       1560 
ESDPVSLERE DKVPGPLEIP SGMEDAGPGA DIIGVNGQGP NLEGKSQHVN GGVMNGLEQS 

      1570       1580       1590       1600       1610       1620 
EEVGQGMPLV SEGDRGSPFQ EEEGSALKTS WAGAPVHLGQ GQFLKFTQRE GDRESWSSGE 


D

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References

« Hide 'large scale' references
[1]"Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments."
Dahlstrand J., McKay R.D.G., Zimmerman L.B., Lendahl U.
J. Cell Sci. 103:589-597(1992) [PubMed: 1478958] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene."
Yaworsky P.J., Kappen C.
Dev. Biol. 205:309-321(1999) [PubMed: 9917366] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 297-310.
[4]"Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma."
Ohira M., Morohashi A., Nakamura Y., Isogai E., Furuya K., Hamano S., Machida T., Aoyama M., Fukumura M., Miyazaki K., Suzuki Y., Sugano S., Hirato J., Nakagawara A.
Cancer Lett. 197:63-68(2003) [PubMed: 12880961] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 604-1621.
Tissue: Neuroblastoma.
[5]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-354; SER-680; SER-768; SER-1409; SER-1418; SER-1617 AND SER-1618, MASS SPECTROMETRY.
Tissue: Epithelium.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-311; THR-315; SER-320; SER-325; THR-338; THR-384; THR-388; SER-398; SER-471; SER-548; SER-564; SER-578; SER-588; SER-746; SER-768; SER-842; THR-851; SER-905; SER-1418; SER-1585; SER-1615; SER-1617 AND SER-1618, MASS SPECTROMETRY.
[7]"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1418, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

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Cross-references

Sequence databases