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P48681

- NEST_HUMAN

UniProt

P48681 - NEST_HUMAN

Protein

Nestin

Gene

NES

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells By similarity.By similarity

    GO - Molecular functioni

    1. intermediate filament binding Source: UniProtKB
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. brain development Source: UniProtKB
    2. cell projection morphogenesis Source: Ensembl
    3. central nervous system development Source: UniProtKB
    4. embryonic camera-type eye development Source: UniProtKB
    5. G2/M transition of mitotic cell cycle Source: DFLAT
    6. negative regulation of catalytic activity Source: UniProtKB
    7. negative regulation of neuron apoptotic process Source: Ensembl
    8. negative regulation of protein binding Source: UniProtKB
    9. positive regulation of intermediate filament depolymerization Source: UniProtKB
    10. positive regulation of neural precursor cell proliferation Source: UniProtKB
    11. stem cell proliferation Source: DFLAT

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Neurogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nestin
    Gene namesi
    Name:NES
    ORF Names:Nbla00170
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:7756. NES.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: DFLAT
    2. intermediate filament Source: UniProtKB
    3. intermediate filament cytoskeleton Source: HPA

    Keywords - Cellular componenti

    Intermediate filament

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31556.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16211621NestinPRO_0000063853Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei311 – 3111Phosphoserine1 Publication
    Modified residuei315 – 3151Phosphothreonine1 Publication
    Modified residuei325 – 3251Phosphoserine2 Publications
    Modified residuei355 – 3551Phosphoserine1 Publication
    Modified residuei358 – 3581Phosphoserine2 Publications
    Modified residuei388 – 3881Phosphothreonine1 Publication
    Modified residuei398 – 3981Phosphoserine2 Publications
    Modified residuei471 – 4711Phosphoserine3 Publications
    Modified residuei548 – 5481Phosphoserine3 Publications
    Modified residuei564 – 5641Phosphoserine1 Publication
    Modified residuei578 – 5781Phosphoserine2 Publications
    Modified residuei588 – 5881Phosphoserine1 Publication
    Modified residuei680 – 6801Phosphoserine3 Publications
    Modified residuei746 – 7461Phosphoserine2 Publications
    Modified residuei768 – 7681Phosphoserine2 Publications
    Modified residuei790 – 7901Phosphoserine1 Publication
    Modified residuei820 – 8201Phosphoserine1 Publication
    Modified residuei831 – 8311Phosphoserine1 Publication
    Modified residuei851 – 8511Phosphothreonine1 Publication
    Modified residuei894 – 8941Phosphoserine1 Publication
    Modified residuei905 – 9051Phosphoserine2 Publications
    Modified residuei913 – 9131Phosphoserine1 Publication
    Modified residuei934 – 9341Phosphoserine1 Publication
    Modified residuei1261 – 12611Phosphoserine1 Publication
    Modified residuei1282 – 12821Phosphoserine1 Publication
    Modified residuei1286 – 12861Phosphoserine1 Publication
    Modified residuei1347 – 13471Phosphoserine1 Publication
    Modified residuei1409 – 14091Phosphoserine1 Publication
    Modified residuei1418 – 14181Phosphoserine4 Publications
    Modified residuei1452 – 14521Phosphoserine1 Publication
    Modified residuei1496 – 14961Phosphoserine1 Publication
    Modified residuei1498 – 14981Phosphoserine1 Publication
    Modified residuei1577 – 15771Phosphoserine1 Publication
    Modified residuei1617 – 16171Phosphoserine1 Publication
    Modified residuei1618 – 16181Phosphoserine1 Publication

    Post-translational modificationi

    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP48681.
    PaxDbiP48681.
    PRIDEiP48681.

    PTM databases

    PhosphoSiteiP48681.

    Expressioni

    Tissue specificityi

    CNS stem cells.

    Developmental stagei

    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

    Gene expression databases

    ArrayExpressiP48681.
    BgeeiP48681.
    CleanExiHS_NES.
    GenevestigatoriP48681.

    Organism-specific databases

    HPAiCAB005889.
    CAB058692.
    HPA007007.
    HPA026111.

    Interactioni

    Subunit structurei

    Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi115983. 5 interactions.
    STRINGi9606.ENSP00000357206.

    Structurei

    3D structure databases

    ProteinModelPortaliP48681.
    SMRiP48681. Positions 7-155, 220-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 77Head
    Regioni8 – 313306RodAdd
    BLAST
    Regioni8 – 4336Coil 1AAdd
    BLAST
    Regioni44 – 5512Linker 1Add
    BLAST
    Regioni56 – 15196Coil 1BAdd
    BLAST
    Regioni152 – 17322Linker 12Add
    BLAST
    Regioni174 – 19219Coil 2AAdd
    BLAST
    Regioni193 – 1953Linker 2
    Regioni196 – 313118Coil 2BAdd
    BLAST
    Regioni314 – 16211308TailAdd
    BLAST

    Sequence similaritiesi

    Belongs to the intermediate filament family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000113766.
    HOVERGENiHBG006463.
    InParanoidiP48681.
    KOiK07609.
    OMAiDHASLAP.
    OrthoDBiEOG773XKP.
    PhylomeDBiP48681.
    TreeFamiTF336633.

    Family and domain databases

    InterProiIPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    [Graphical view]
    PANTHERiPTHR23239. PTHR23239. 1 hit.
    PfamiPF00038. Filament. 2 hits.
    [Graphical view]
    PROSITEiPS00226. IF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48681-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGCMGEESF QMWELNRRLE AYLARVKALE EQNELLSAEL GGLRAQSADT     50
    SWRAHADDEL AALRALVDQR WREKHAAEVA RDNLAEELEG VAGRCQQLRL 100
    ARERTTEEVA RNRRAVEAEK CARAWLSSQV AELERELEAL RVAHEEERVG 150
    LNAQAACAPR CPAPPRGPPA PAPEVEELAR RLGEAWRGAV RGYQERVAHM 200
    ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA ALEQRLEGRW 250
    QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA 300
    TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV 350
    LSPTSLPSPL PATLETPVPA FLKNQEFLQA RTPTLASTPI PPTPQAPSPA 400
    VDAEIRAQDA PLSLLQTQGG RKQAPEPLRA EARVAIPASV LPGPEEPGGQ 450
    RQEASTGQSP EDHASLAPPL SPDHSSLEAK DGESGGSRVF SICRGEGEGQ 500
    IWGLVEKETA IEGKVVSSLQ QEIWEEEDLN RKEIQDSQVP LEKETLKSLG 550
    EEIQESLKTL ENQSHETLER ENQECPRSLE EDLETLKSLE KENKELLKDV 600
    EVVRPLEKEA VGQLKPTGKE DTQTLQSLQK ENQELMKSLE GNLETFLFPG 650
    TENQELVSSL QENLESLTAL EKENQEPLRS PEVGDEEALR PLTKENQEPL 700
    RSLEDENKEA FRSLEKENQE PLKTLEEEDQ SIVRPLETEN HKSLRSLEEQ 750
    DQETLRTLEK ETQQRRRSLG EQDQMTLRPP EKVDLEPLKS LDQEIARPLE 800
    NENQEFLKSL KEESVEAVKS LETEILESLK SAGQENLETL KSPETQAPLW 850
    TPEEINQGAM NPLEKEIQEP LESVEVNQET FRLLEEENQE SLRSLGAWNL 900
    ENLRSPEEVD KESQRNLEEE ENLGKGEYQE SLRSLEEEGQ ELPQSADVQR 950
    WEDTVEKDQE LAQESPPGMA GVENEDEAEL NLREQDGFTG KEEVVEQGEL 1000
    NATEEVWIPG EGHPESPEPK EQRGLVEGAS VKGGAEGLQD PEGQSQQVGA 1050
    PGLQAPQGLP EAIEPLVEDD VAPGGDQASP EVMLGSEPAM GESAAGAEPG 1100
    PGQGVGGLGD PGHLTREEVM EPPLEEESLE AKRVQGLEGP RKDLEEAGGL 1150
    GTEFSELPGK SRDPWEPPRE GREESEAEAP RGAEEAFPAE TLGHTGSDAP 1200
    SPWPLGSEEA EEDVPPVLVS PSPTYTPILE DAPGPQPQAE GSQEASWGVQ 1250
    GRAEALGKVE SEQEELGSGE IPEGPQEEGE ESREESEEDE LGETLPDSTP 1300
    LGFYLRSPTS PRWDPTGEQR PPPQGETGKE GWDPAVLASE GLEAPPSEKE 1350
    EGEEGEEECG RDSDLSEEFE DLGTEAPFLP GVPGEVAEPL GQVPQLLLDP 1400
    AAWDRDGESD GFADEEESGE EGEEDQEEGR EPGAGRWGPG SSVGSLQALS 1450
    SSQRGEFLES DSVSVSVPWD DSLRGAVAGA PKTALETESQ DSAEPSGSEE 1500
    ESDPVSLERE DKVPGPLEIP SGMEDAGPGA DIIGVNGQGP NLEGKSQHVN 1550
    GGVMNGLEQS EEVGQGMPLV SEGDRGSPFQ EEEGSALKTS WAGAPVHLGQ 1600
    GQFLKFTQRE GDRESWSSGE D 1621
    Length:1,621
    Mass (Da):177,439
    Last modified:May 1, 2007 - v2
    Checksum:i0E4F967C11F44C13
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241A → G in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti39 – 391E → G in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti45 – 451A → R in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti96 – 961Q → E in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti130 – 1301V → G in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti161 – 1611C → L in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti167 – 1671Missing in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti205 – 2073GQA → DQT in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti212 – 2121G → A in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti221 – 2211G → V in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti595 – 61218ELLKD…KEAVG → RAIKGCGGSETSRKRGCR in CAA46780. (PubMed:1478958)CuratedAdd
    BLAST
    Sequence conflicti857 – 90852QGAMN…RSPEE → KSGGNESSRKGNSRTTGVCG SEPRDIQTPGRGESGIIEIS GSMEPGEFEISRG in CAA46780. (PubMed:1478958)CuratedAdd
    BLAST
    Sequence conflicti975 – 9751E → K in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti1008 – 10081I → F in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti1050 – 10501A → T in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti1235 – 12351P → L in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti1256 – 12561Missing in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti1314 – 132512DPTGE…PPPQG → TPLESRGHPLK in CAA46780. (PubMed:1478958)CuratedAdd
    BLAST
    Sequence conflicti1344 – 13452AP → E in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti1472 – 14721S → N in BAE45713. (PubMed:12880961)Curated
    Sequence conflicti1542 – 15421L → M in BAE45713. (PubMed:12880961)Curated
    Sequence conflicti1563 – 15686VGQGMP → SGARNA in CAA46780. (PubMed:1478958)Curated
    Sequence conflicti1589 – 15913TSW → RSS in CAA46780. (PubMed:1478958)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti130 – 1301V → A.
    Corresponds to variant rs4278369 [ dbSNP | Ensembl ].
    VAR_061301
    Natural varianti815 – 8151V → I.1 Publication
    Corresponds to variant rs951781 [ dbSNP | Ensembl ].
    VAR_049814
    Natural varianti1016 – 10161S → N.1 Publication
    Corresponds to variant rs2365718 [ dbSNP | Ensembl ].
    VAR_049815
    Natural varianti1101 – 11011P → L.1 Publication
    Corresponds to variant rs2886443 [ dbSNP | Ensembl ].
    VAR_049816
    Natural varianti1133 – 11331R → S.
    Corresponds to variant rs17393797 [ dbSNP | Ensembl ].
    VAR_049817
    Natural varianti1275 – 12751P → L.3 Publications
    Corresponds to variant rs3748570 [ dbSNP | Ensembl ].
    VAR_049818

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590666 Genomic DNA. Translation: CAI16338.1.
    X65964 Genomic DNA. Translation: CAA46780.1.
    AF004335 Genomic DNA. Translation: AAB64426.1.
    AB073350 mRNA. Translation: BAE45713.1.
    CCDSiCCDS1151.1.
    PIRiS21424.
    RefSeqiNP_006608.1. NM_006617.1.
    UniGeneiHs.527971.

    Genome annotation databases

    EnsembliENST00000368223; ENSP00000357206; ENSG00000132688.
    GeneIDi10763.
    KEGGihsa:10763.
    UCSCiuc001fpq.3. human.

    Polymorphism databases

    DMDMi146345464.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590666 Genomic DNA. Translation: CAI16338.1 .
    X65964 Genomic DNA. Translation: CAA46780.1 .
    AF004335 Genomic DNA. Translation: AAB64426.1 .
    AB073350 mRNA. Translation: BAE45713.1 .
    CCDSi CCDS1151.1.
    PIRi S21424.
    RefSeqi NP_006608.1. NM_006617.1.
    UniGenei Hs.527971.

    3D structure databases

    ProteinModelPortali P48681.
    SMRi P48681. Positions 7-155, 220-311.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115983. 5 interactions.
    STRINGi 9606.ENSP00000357206.

    PTM databases

    PhosphoSitei P48681.

    Polymorphism databases

    DMDMi 146345464.

    Proteomic databases

    MaxQBi P48681.
    PaxDbi P48681.
    PRIDEi P48681.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000368223 ; ENSP00000357206 ; ENSG00000132688 .
    GeneIDi 10763.
    KEGGi hsa:10763.
    UCSCi uc001fpq.3. human.

    Organism-specific databases

    CTDi 10763.
    GeneCardsi GC01M156639.
    H-InvDB HIX0001169.
    HGNCi HGNC:7756. NES.
    HPAi CAB005889.
    CAB058692.
    HPA007007.
    HPA026111.
    MIMi 600915. gene.
    neXtProti NX_P48681.
    PharmGKBi PA31556.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000113766.
    HOVERGENi HBG006463.
    InParanoidi P48681.
    KOi K07609.
    OMAi DHASLAP.
    OrthoDBi EOG773XKP.
    PhylomeDBi P48681.
    TreeFami TF336633.

    Miscellaneous databases

    GeneWikii Nestin_(protein).
    GenomeRNAii 10763.
    NextBioi 40873.
    PROi P48681.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48681.
    Bgeei P48681.
    CleanExi HS_NES.
    Genevestigatori P48681.

    Family and domain databases

    InterProi IPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    [Graphical view ]
    PANTHERi PTHR23239. PTHR23239. 1 hit.
    Pfami PF00038. Filament. 2 hits.
    [Graphical view ]
    PROSITEi PS00226. IF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments."
      Dahlstrand J., McKay R.D.G., Zimmerman L.B., Lendahl U.
      J. Cell Sci. 103:589-597(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ASN-1016; LEU-1101 AND LEU-1275.
      Tissue: Placenta.
    2. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene."
      Yaworsky P.J., Kappen C.
      Dev. Biol. 205:309-321(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 297-310.
    4. "Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma."
      Ohira M., Morohashi A., Nakamura Y., Isogai E., Furuya K., Hamano S., Machida T., Aoyama M., Fukumura M., Miyazaki K., Suzuki Y., Sugano S., Hirato J., Nakagawara A.
      Cancer Lett. 197:63-68(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 604-1621, VARIANT ILE-815.
      Tissue: Neuroblastoma.
    5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680; SER-1409 AND SER-1418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-311; THR-315; SER-325; THR-388; SER-398; SER-471; SER-548; SER-564; SER-578; SER-588; SER-746; SER-768; THR-851; SER-905 AND SER-1418, VARIANT [LARGE SCALE ANALYSIS] LEU-1275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
      Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
      Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; SER-398; SER-471; SER-548 AND SER-680, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-325; SER-355; SER-358; SER-471; SER-548; SER-578; SER-680; SER-746; SER-768; SER-790; SER-820; SER-831; SER-894; SER-905; SER-913; SER-934; SER-1261; SER-1282; SER-1286; SER-1347; SER-1418; SER-1452; SER-1496; SER-1498; SER-1577; SER-1617 AND SER-1618, VARIANT [LARGE SCALE ANALYSIS] LEU-1275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNEST_HUMAN
    AccessioniPrimary (citable) accession number: P48681
    Secondary accession number(s): O00552, Q3LIF5, Q5SYZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3