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P48664

- EAA4_HUMAN

UniProt

P48664 - EAA4_HUMAN

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Protein

Excitatory amino acid transporter 4

Gene
SLC1A6, EAAT4
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Seems to act as a symport by cotransporting sodium.

GO - Molecular functioni

  1. L-aspartate transmembrane transporter activity Source: ProtInc
  2. L-glutamate transmembrane transporter activity Source: Ensembl
  3. sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  1. aspartate transport Source: ProtInc
  2. ion transport Source: Reactome
  3. L-glutamate transport Source: ProtInc
  4. regulation of membrane potential Source: Ensembl
  5. synaptic transmission Source: ProtInc
  6. transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.

Protein family/group databases

TCDBi2.A.23.2.8. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 4
Alternative name(s):
Sodium-dependent glutamate/aspartate transporter
Solute carrier family 1 member 6
Gene namesi
Name:SLC1A6
Synonyms:EAAT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:10944. SLC1A6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei56 – 7621Helical; Reviewed predictionAdd
BLAST
Transmembranei99 – 11921Helical; Reviewed predictionAdd
BLAST
Transmembranei133 – 15321Helical; Reviewed predictionAdd
BLAST
Topological domaini154 – 264111Extracellular Reviewed predictionAdd
BLAST
Transmembranei265 – 28521Helical; Reviewed predictionAdd
BLAST
Transmembranei306 – 32621Helical; Reviewed predictionAdd
BLAST
Transmembranei345 – 36521Helical; Reviewed predictionAdd
BLAST
Transmembranei370 – 39021Helical; Reviewed predictionAdd
BLAST
Transmembranei418 – 43821Helical; Reviewed predictionAdd
BLAST
Transmembranei460 – 48021Helical; Reviewed predictionAdd
BLAST
Transmembranei481 – 50121Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: Ensembl
  2. integral component of plasma membrane Source: ProtInc
  3. membrane Source: Ensembl
  4. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35831.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Excitatory amino acid transporter 4PRO_0000202070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi216 – 2161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi232 – 2321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi239 – 2391N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP48664.
PRIDEiP48664.

PTM databases

PhosphoSiteiP48664.

Expressioni

Tissue specificityi

Brain. Expressed densely and selectively in cell bodies of Purkinje cells.

Gene expression databases

ArrayExpressiP48664.
BgeeiP48664.
CleanExiHS_SLC1A6.
GenevestigatoriP48664.

Organism-specific databases

HPAiHPA041505.
HPA044066.

Interactioni

Protein-protein interaction databases

BioGridi112402. 1 interaction.
IntActiP48664. 1 interaction.
STRINGi9606.ENSP00000221742.

Structurei

3D structure databases

ProteinModelPortaliP48664.
SMRiP48664. Positions 65-189, 250-519.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP48664.
KOiK05617.
OMAiGFSIVRF.
OrthoDBiEOG7RV9G2.
PhylomeDBiP48664.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P48664-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSHGNSLFL RESGQRLGRV GWLQRLQESL QQRALRTRLR LQTMTLEHVL    50
RFLRRNAFIL LTVSAVVIGV SLAFALRPYQ LTYRQIKYFS FPGELLMRML 100
QMLVLPLIVS SLVTGMASLD NKATGRMGMR AAVYYMVTTI IAVFIGILMV 150
TIIHPGKGSK EGLHREGRIE TIPTADAFMD LIRNMFPPNL VEACFKQFKT 200
QYSTRVVTRT MVRTENGSEP GASMPPPFSV ENGTSFLENV TRALGTLQEM 250
LSFEETVPVP GSANGINALG LVVFSVAFGL VIGGMKHKGR VLRDFFDSLN 300
EAIMRLVGII IWYAPVGILF LIAGKILEME DMAVLGGQLG MYTLTVIVGL 350
FLHAGIVLPL IYFLVTHRNP FPFIGGMLQA LITAMGTSSS SATLPITFRC 400
LEEGLGVDRR ITRFVLPVGA TVNMDGTALY EALAAIFIAQ VNNYELNLGQ 450
ITTISITATA ASVGAAGIPQ AGLVTMVIVL TSVGLPTEDI TLIIAVDWFL 500
DRLRTMTNVL GDSIGAAVIE HLSQRELELQ EAELTLPSLG KPYKSLMAQE 550
KGASRGRGGN ESAM 564
Length:564
Mass (Da):61,565
Last modified:February 1, 1996 - v1
Checksum:iBB6700D6EAEC2B20
GO
Isoform 2 (identifier: P48664-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     313-564: Missing.

Show »
Length:312
Mass (Da):34,846
Checksum:i0C06E0DEC9A864C6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei313 – 564252Missing in isoform 2. VSP_055130Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18244 mRNA. Translation: AAA75314.1.
AC004659 Genomic DNA. Translation: AAC15754.1.
AC104528 Genomic DNA. No translation available.
BC028721 mRNA. Translation: AAH28721.1.
BC040604 mRNA. Translation: AAH40604.1.
CCDSiCCDS12321.1. [P48664-1]
PIRiS58398.
RefSeqiNP_001259016.1. NM_001272087.1.
NP_001259017.1. NM_001272088.1.
NP_005062.1. NM_005071.2.
XP_006722905.1. XM_006722842.1.
XP_006722906.1. XM_006722843.1.
XP_006722907.1. XM_006722844.1.
UniGeneiHs.515217.
Hs.733178.

Genome annotation databases

EnsembliENST00000221742; ENSP00000221742; ENSG00000105143.
ENST00000544886; ENSP00000446175; ENSG00000105143.
ENST00000598504; ENSP00000471781; ENSG00000105143.
GeneIDi6511.
KEGGihsa:6511.
UCSCiuc002naa.2. human. [P48664-1]

Polymorphism databases

DMDMi1352333.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18244 mRNA. Translation: AAA75314.1 .
AC004659 Genomic DNA. Translation: AAC15754.1 .
AC104528 Genomic DNA. No translation available.
BC028721 mRNA. Translation: AAH28721.1 .
BC040604 mRNA. Translation: AAH40604.1 .
CCDSi CCDS12321.1. [P48664-1 ]
PIRi S58398.
RefSeqi NP_001259016.1. NM_001272087.1.
NP_001259017.1. NM_001272088.1.
NP_005062.1. NM_005071.2.
XP_006722905.1. XM_006722842.1.
XP_006722906.1. XM_006722843.1.
XP_006722907.1. XM_006722844.1.
UniGenei Hs.515217.
Hs.733178.

3D structure databases

ProteinModelPortali P48664.
SMRi P48664. Positions 65-189, 250-519.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112402. 1 interaction.
IntActi P48664. 1 interaction.
STRINGi 9606.ENSP00000221742.

Chemistry

BindingDBi P48664.
ChEMBLi CHEMBL3173.
DrugBanki DB00142. L-Glutamic Acid.
GuidetoPHARMACOLOGYi 871.

Protein family/group databases

TCDBi 2.A.23.2.8. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

PhosphoSitei P48664.

Polymorphism databases

DMDMi 1352333.

Proteomic databases

PaxDbi P48664.
PRIDEi P48664.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000221742 ; ENSP00000221742 ; ENSG00000105143 .
ENST00000544886 ; ENSP00000446175 ; ENSG00000105143 .
ENST00000598504 ; ENSP00000471781 ; ENSG00000105143 .
GeneIDi 6511.
KEGGi hsa:6511.
UCSCi uc002naa.2. human. [P48664-1 ]

Organism-specific databases

CTDi 6511.
GeneCardsi GC19M015060.
HGNCi HGNC:10944. SLC1A6.
HPAi HPA041505.
HPA044066.
MIMi 600637. gene.
neXtProti NX_P48664.
PharmGKBi PA35831.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1301.
HOGENOMi HOG000208776.
HOVERGENi HBG000080.
InParanoidi P48664.
KOi K05617.
OMAi GFSIVRF.
OrthoDBi EOG7RV9G2.
PhylomeDBi P48664.
TreeFami TF315206.

Enzyme and pathway databases

Reactomei REACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

GeneWikii Excitatory_amino-acid_transporter_4.
GenomeRNAii 6511.
NextBioi 25319.
PROi P48664.
SOURCEi Search...

Gene expression databases

ArrayExpressi P48664.
Bgeei P48664.
CleanExi HS_SLC1A6.
Genevestigatori P48664.

Family and domain databases

Gene3Di 1.10.3860.10. 2 hits.
InterProi IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view ]
PANTHERi PTHR11958. PTHR11958. 1 hit.
Pfami PF00375. SDF. 1 hit.
[Graphical view ]
PRINTSi PR00173. EDTRNSPORT.
SUPFAMi SSF118215. SSF118215. 2 hits.
PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An excitatory amino-acid transporter with properties of a ligand-gated chloride channel."
    Fairman W.A., Vandenberg R.J., Arriza J.L., Kavanaugh M.P., Amara S.G.
    Nature 375:599-603(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.

Entry informationi

Entry nameiEAA4_HUMAN
AccessioniPrimary (citable) accession number: P48664
Secondary accession number(s): Q8N753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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