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P48664 (EAA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Excitatory amino acid transporter 4
Alternative name(s):
Sodium-dependent glutamate/aspartate transporter
Solute carrier family 1 member 6
Gene names
Name:SLC1A6
Synonyms:EAAT4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transports L-glutamate and also L- and D-aspartate. Seems to act as a symport by cotransporting sodium.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Brain. Expressed densely and selectively in cell bodies of Purkinje cells.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A6 subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P48664-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P48664-2)

The sequence of this isoform differs from the canonical sequence as follows:
     313-564: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564Excitatory amino acid transporter 4
PRO_0000202070

Regions

Topological domain1 – 5555Cytoplasmic Potential
Transmembrane56 – 7621Helical; Potential
Transmembrane99 – 11921Helical; Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 264111Extracellular Potential
Transmembrane265 – 28521Helical; Potential
Transmembrane306 – 32621Helical; Potential
Transmembrane345 – 36521Helical; Potential
Transmembrane370 – 39021Helical; Potential
Transmembrane418 – 43821Helical; Potential
Transmembrane460 – 48021Helical; Potential
Transmembrane481 – 50121Helical; Potential

Amino acid modifications

Glycosylation2161N-linked (GlcNAc...) Potential
Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2391N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence313 – 564252Missing in isoform 2.
VSP_055130

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: BB6700D6EAEC2B20

FASTA56461,565
        10         20         30         40         50         60 
MSSHGNSLFL RESGQRLGRV GWLQRLQESL QQRALRTRLR LQTMTLEHVL RFLRRNAFIL 

        70         80         90        100        110        120 
LTVSAVVIGV SLAFALRPYQ LTYRQIKYFS FPGELLMRML QMLVLPLIVS SLVTGMASLD 

       130        140        150        160        170        180 
NKATGRMGMR AAVYYMVTTI IAVFIGILMV TIIHPGKGSK EGLHREGRIE TIPTADAFMD 

       190        200        210        220        230        240 
LIRNMFPPNL VEACFKQFKT QYSTRVVTRT MVRTENGSEP GASMPPPFSV ENGTSFLENV 

       250        260        270        280        290        300 
TRALGTLQEM LSFEETVPVP GSANGINALG LVVFSVAFGL VIGGMKHKGR VLRDFFDSLN 

       310        320        330        340        350        360 
EAIMRLVGII IWYAPVGILF LIAGKILEME DMAVLGGQLG MYTLTVIVGL FLHAGIVLPL 

       370        380        390        400        410        420 
IYFLVTHRNP FPFIGGMLQA LITAMGTSSS SATLPITFRC LEEGLGVDRR ITRFVLPVGA 

       430        440        450        460        470        480 
TVNMDGTALY EALAAIFIAQ VNNYELNLGQ ITTISITATA ASVGAAGIPQ AGLVTMVIVL 

       490        500        510        520        530        540 
TSVGLPTEDI TLIIAVDWFL DRLRTMTNVL GDSIGAAVIE HLSQRELELQ EAELTLPSLG 

       550        560 
KPYKSLMAQE KGASRGRGGN ESAM 

« Hide

Isoform 2 [UniParc].

Checksum: 0C06E0DEC9A864C6
Show »

FASTA31234,846

References

« Hide 'large scale' references
[1]"An excitatory amino-acid transporter with properties of a ligand-gated chloride channel."
Fairman W.A., Vandenberg R.J., Arriza J.L., Kavanaugh M.P., Amara S.G.
Nature 375:599-603(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18244 mRNA. Translation: AAA75314.1.
AC004659 Genomic DNA. Translation: AAC15754.1.
AC104528 Genomic DNA. No translation available.
BC028721 mRNA. Translation: AAH28721.1.
BC040604 mRNA. Translation: AAH40604.1.
CCDSCCDS12321.1.
PIRS58398.
RefSeqNP_005062.1. NM_005071.2.
XP_006722905.1. XM_006722842.1.
XP_006722906.1. XM_006722843.1.
XP_006722907.1. XM_006722844.1.
UniGeneHs.515217.
Hs.733178.

3D structure databases

ProteinModelPortalP48664.
SMRP48664. Positions 65-189, 250-519.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112402. 1 interaction.
IntActP48664. 1 interaction.
STRING9606.ENSP00000221742.

Chemistry

BindingDBP48664.
ChEMBLCHEMBL3173.
DrugBankDB00142. L-Glutamic Acid.
GuidetoPHARMACOLOGY871.

Protein family/group databases

TCDB2.A.23.2.8. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

PhosphoSiteP48664.

Polymorphism databases

DMDM1352333.

Proteomic databases

PaxDbP48664.
PRIDEP48664.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000221742; ENSP00000221742; ENSG00000105143.
ENST00000544886; ENSP00000446175; ENSG00000105143.
ENST00000598504; ENSP00000471781; ENSG00000105143.
GeneID6511.
KEGGhsa:6511.
UCSCuc002naa.2. human.

Organism-specific databases

CTD6511.
GeneCardsGC19M015060.
HGNCHGNC:10944. SLC1A6.
HPAHPA041505.
HPA044066.
MIM600637. gene.
neXtProtNX_P48664.
PharmGKBPA35831.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1301.
HOGENOMHOG000208776.
HOVERGENHBG000080.
InParanoidP48664.
KOK05617.
OMAGFSIVRF.
OrthoDBEOG7RV9G2.
PhylomeDBP48664.
TreeFamTF315206.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_19419. Amino acid and oligopeptide SLC transporters.

Gene expression databases

ArrayExpressP48664.
BgeeP48664.
CleanExHS_SLC1A6.
GenevestigatorP48664.

Family and domain databases

Gene3D1.10.3860.10. 2 hits.
InterProIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERPTHR11958. PTHR11958. 1 hit.
PfamPF00375. SDF. 1 hit.
[Graphical view]
PRINTSPR00173. EDTRNSPORT.
SUPFAMSSF118215. SSF118215. 2 hits.
PROSITEPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiExcitatory_amino-acid_transporter_4.
GenomeRNAi6511.
NextBio25319.
PROP48664.
SOURCESearch...

Entry information

Entry nameEAA4_HUMAN
AccessionPrimary (citable) accession number: P48664
Secondary accession number(s): Q8N753
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM