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Protein

Excitatory amino acid transporter 4

Gene

SLC1A6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Seems to act as a symport by cotransporting sodium.

GO - Molecular functioni

GO - Biological processi

  • aspartate transport Source: ProtInc
  • glutamate secretion Source: Reactome
  • ion transport Source: Reactome
  • L-aspartate transmembrane transport Source: GOC
  • L-glutamate transport Source: ProtInc
  • neurotransmitter secretion Source: Reactome
  • regulation of membrane potential Source: Ensembl
  • synaptic transmission Source: Reactome
  • transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.

Protein family/group databases

TCDBi2.A.23.2.8. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 4
Alternative name(s):
Sodium-dependent glutamate/aspartate transporter
Solute carrier family 1 member 6
Gene namesi
Name:SLC1A6
Synonyms:EAAT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:10944. SLC1A6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei56 – 7621HelicalSequence AnalysisAdd
BLAST
Transmembranei99 – 11921HelicalSequence AnalysisAdd
BLAST
Transmembranei133 – 15321HelicalSequence AnalysisAdd
BLAST
Topological domaini154 – 264111ExtracellularSequence AnalysisAdd
BLAST
Transmembranei265 – 28521HelicalSequence AnalysisAdd
BLAST
Transmembranei306 – 32621HelicalSequence AnalysisAdd
BLAST
Transmembranei345 – 36521HelicalSequence AnalysisAdd
BLAST
Transmembranei370 – 39021HelicalSequence AnalysisAdd
BLAST
Transmembranei418 – 43821HelicalSequence AnalysisAdd
BLAST
Transmembranei460 – 48021HelicalSequence AnalysisAdd
BLAST
Transmembranei481 – 50121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: Ensembl
  • integral component of plasma membrane Source: ProtInc
  • membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35831.

Polymorphism and mutation databases

DMDMi1352333.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Excitatory amino acid transporter 4PRO_0000202070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP48664.
PRIDEiP48664.

PTM databases

PhosphoSiteiP48664.

Expressioni

Tissue specificityi

Brain. Expressed densely and selectively in cell bodies of Purkinje cells.

Gene expression databases

BgeeiP48664.
CleanExiHS_SLC1A6.
ExpressionAtlasiP48664. baseline and differential.
GenevisibleiP48664. HS.

Organism-specific databases

HPAiHPA041505.
HPA044066.

Interactioni

Protein-protein interaction databases

BioGridi112402. 1 interaction.
IntActiP48664. 1 interaction.
STRINGi9606.ENSP00000221742.

Structurei

3D structure databases

ProteinModelPortaliP48664.
SMRiP48664. Positions 65-189, 250-519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP48664.
KOiK05617.
OMAiDVEHYNA.
OrthoDBiEOG7RV9G2.
PhylomeDBiP48664.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P48664-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSHGNSLFL RESGQRLGRV GWLQRLQESL QQRALRTRLR LQTMTLEHVL
60 70 80 90 100
RFLRRNAFIL LTVSAVVIGV SLAFALRPYQ LTYRQIKYFS FPGELLMRML
110 120 130 140 150
QMLVLPLIVS SLVTGMASLD NKATGRMGMR AAVYYMVTTI IAVFIGILMV
160 170 180 190 200
TIIHPGKGSK EGLHREGRIE TIPTADAFMD LIRNMFPPNL VEACFKQFKT
210 220 230 240 250
QYSTRVVTRT MVRTENGSEP GASMPPPFSV ENGTSFLENV TRALGTLQEM
260 270 280 290 300
LSFEETVPVP GSANGINALG LVVFSVAFGL VIGGMKHKGR VLRDFFDSLN
310 320 330 340 350
EAIMRLVGII IWYAPVGILF LIAGKILEME DMAVLGGQLG MYTLTVIVGL
360 370 380 390 400
FLHAGIVLPL IYFLVTHRNP FPFIGGMLQA LITAMGTSSS SATLPITFRC
410 420 430 440 450
LEEGLGVDRR ITRFVLPVGA TVNMDGTALY EALAAIFIAQ VNNYELNLGQ
460 470 480 490 500
ITTISITATA ASVGAAGIPQ AGLVTMVIVL TSVGLPTEDI TLIIAVDWFL
510 520 530 540 550
DRLRTMTNVL GDSIGAAVIE HLSQRELELQ EAELTLPSLG KPYKSLMAQE
560
KGASRGRGGN ESAM
Length:564
Mass (Da):61,565
Last modified:February 1, 1996 - v1
Checksum:iBB6700D6EAEC2B20
GO
Isoform 2 (identifier: P48664-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     313-564: Missing.

Show »
Length:312
Mass (Da):34,846
Checksum:i0C06E0DEC9A864C6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei313 – 564252Missing in isoform 2. 1 PublicationVSP_055130Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18244 mRNA. Translation: AAA75314.1.
AC004659 Genomic DNA. Translation: AAC15754.1.
AC104528 Genomic DNA. No translation available.
BC028721 mRNA. Translation: AAH28721.1.
BC040604 mRNA. Translation: AAH40604.1.
CCDSiCCDS12321.1. [P48664-1]
CCDS62578.1. [P48664-2]
PIRiS58398.
RefSeqiNP_001259016.1. NM_001272087.1. [P48664-2]
NP_001259017.1. NM_001272088.1. [P48664-2]
NP_005062.1. NM_005071.2. [P48664-1]
XP_006722905.1. XM_006722842.1. [P48664-1]
XP_006722906.1. XM_006722843.2. [P48664-1]
XP_006722907.1. XM_006722844.1. [P48664-1]
UniGeneiHs.515217.
Hs.733178.

Genome annotation databases

EnsembliENST00000221742; ENSP00000221742; ENSG00000105143. [P48664-1]
ENST00000544886; ENSP00000446175; ENSG00000105143. [P48664-2]
ENST00000598504; ENSP00000471781; ENSG00000105143. [P48664-2]
GeneIDi6511.
KEGGihsa:6511.
UCSCiuc002naa.2. human. [P48664-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18244 mRNA. Translation: AAA75314.1.
AC004659 Genomic DNA. Translation: AAC15754.1.
AC104528 Genomic DNA. No translation available.
BC028721 mRNA. Translation: AAH28721.1.
BC040604 mRNA. Translation: AAH40604.1.
CCDSiCCDS12321.1. [P48664-1]
CCDS62578.1. [P48664-2]
PIRiS58398.
RefSeqiNP_001259016.1. NM_001272087.1. [P48664-2]
NP_001259017.1. NM_001272088.1. [P48664-2]
NP_005062.1. NM_005071.2. [P48664-1]
XP_006722905.1. XM_006722842.1. [P48664-1]
XP_006722906.1. XM_006722843.2. [P48664-1]
XP_006722907.1. XM_006722844.1. [P48664-1]
UniGeneiHs.515217.
Hs.733178.

3D structure databases

ProteinModelPortaliP48664.
SMRiP48664. Positions 65-189, 250-519.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112402. 1 interaction.
IntActiP48664. 1 interaction.
STRINGi9606.ENSP00000221742.

Chemistry

ChEMBLiCHEMBL3173.
GuidetoPHARMACOLOGYi871.

Protein family/group databases

TCDBi2.A.23.2.8. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

PhosphoSiteiP48664.

Polymorphism and mutation databases

DMDMi1352333.

Proteomic databases

PaxDbiP48664.
PRIDEiP48664.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221742; ENSP00000221742; ENSG00000105143. [P48664-1]
ENST00000544886; ENSP00000446175; ENSG00000105143. [P48664-2]
ENST00000598504; ENSP00000471781; ENSG00000105143. [P48664-2]
GeneIDi6511.
KEGGihsa:6511.
UCSCiuc002naa.2. human. [P48664-1]

Organism-specific databases

CTDi6511.
GeneCardsiGC19M015060.
HGNCiHGNC:10944. SLC1A6.
HPAiHPA041505.
HPA044066.
MIMi600637. gene.
neXtProtiNX_P48664.
PharmGKBiPA35831.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP48664.
KOiK05617.
OMAiDVEHYNA.
OrthoDBiEOG7RV9G2.
PhylomeDBiP48664.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

GeneWikiiExcitatory_amino-acid_transporter_4.
GenomeRNAii6511.
NextBioi25319.
PROiP48664.
SOURCEiSearch...

Gene expression databases

BgeeiP48664.
CleanExiHS_SLC1A6.
ExpressionAtlasiP48664. baseline and differential.
GenevisibleiP48664. HS.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An excitatory amino-acid transporter with properties of a ligand-gated chloride channel."
    Fairman W.A., Vandenberg R.J., Arriza J.L., Kavanaugh M.P., Amara S.G.
    Nature 375:599-603(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.

Entry informationi

Entry nameiEAA4_HUMAN
AccessioniPrimary (citable) accession number: P48664
Secondary accession number(s): Q8N753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.