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Protein

Retinal dehydrogenase 1

Gene

ALDH1A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal. May have a broader specificity and oxidize other aldehydes in vivo.1 Publication

Catalytic activityi

Retinal + NAD+ + H2O = retinoate + NADH.1 Publication

Kineticsi

Has a much higher catalytic efficiency toward all-trans retinal.1 Publication

Manual assertion based on experiment ini

  1. KM=9 µM for all-trans retinal1 Publication
  2. KM=600 µM for propionaldehyde1 Publication
  1. Vmax=106 pmol/min/mg enzyme with all-trans retinal1 Publication
  2. Vmax=4650 pmol/min/mg enzyme with all-trans propionaldehyde1 Publication

Pathwayi: retinol metabolism

This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei170Transition state stabilizerBy similarity1
Active sitei269Proton acceptorPROSITE-ProRule annotation1
Active sitei303NucleophilePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 170NADBy similarity4
Nucleotide bindingi193 – 196NADBy similarity4
Nucleotide bindingi226 – 227NADBy similarity2
Nucleotide bindingi246 – 247NADBy similarity2
Nucleotide bindingi269 – 271NADBy similarity3
Nucleotide bindingi349 – 353NADBy similarity5
Nucleotide bindingi400 – 402NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-BTA-5365859. RA biosynthesis pathway.
R-BTA-70350. Fructose catabolism.
R-BTA-71384. Ethanol oxidation.
UniPathwayiUPA00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinal dehydrogenase 1Curated (EC:1.2.1.-1 Publication, EC:1.2.1.361 Publication)
Short name:
RALDH 1Curated
Short name:
RalDH1Curated
Alternative name(s):
Aldehyde dehydrogenase, cytosolicCurated
Gene namesi
Name:ALDH1A1By similarity
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

  • Cytoplasmcytosol 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000564132 – 501Retinal dehydrogenase 1Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei91N6-acetyllysineBy similarity1
Modified residuei128N6-acetyllysineBy similarity1
Modified residuei252N6-acetyllysineBy similarity1
Modified residuei337PhosphothreonineBy similarity1
Modified residuei353N6-acetyllysineBy similarity1
Modified residuei367N6-acetyllysineBy similarity1
Modified residuei410N6-acetyllysineBy similarity1
Modified residuei413PhosphoserineBy similarity1
Modified residuei419N6-acetyllysineBy similarity1
Modified residuei495N6-acetyllysineBy similarity1

Post-translational modificationi

The N-terminus is blocked most probably by acetylation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP48644.
PeptideAtlasiP48644.
PRIDEiP48644.

Expressioni

Tissue specificityi

Expressed in muscle, liver, small intestine, kidney, brain, lung, heart but not detected in erythrocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSBTAG00000008103.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010661.

Structurei

3D structure databases

ProteinModelPortaliP48644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiKOG2450. Eukaryota.
COG1012. LUCA.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP48644.
KOiK07249.
OMAiEWHSSVS.
OrthoDBiEOG091G05E8.
TreeFamiTF300455.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSAMPDVP APLTNLQFKY TKIFINNEWH SSVSGKKFPV FNPATEEKLC
60 70 80 90 100
EVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLN KLADLIERDH
110 120 130 140 150
LLLATMEAMN GGKLFSNAYL MDLGGCIKTL RYCAGWADKI QGRTIPMDGN
160 170 180 190 200
FFTYTRSEPV GVCGQIIPWN FPLLMFLWKI GPALSCGNTV VVKPAEQTPL
210 220 230 240 250
TALHMGSLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD KVAFTGSTEV
260 270 280 290 300
GKLIKEAAGK SNLKRVSLEL GGKSPCIVFA DADLDNAVEF AHQGVFYHQG
310 320 330 340 350
QCCIAASRLF VEESIYDEFV RRSVERAKKY VLGNPLTPGV SQGPQIDKEQ
360 370 380 390 400
YEKILDLIES GKKEGAKLEC GGGPWGNKGY FIQPTVFSDV TDDMRIAKEE
410 420 430 440 450
IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GIFTNDIDKA ITVSSALQSG
460 470 480 490 500
TVWVNCYSVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTIKISQKN

S
Length:501
Mass (Da):54,806
Last modified:November 13, 2007 - v3
Checksum:iC9025D7034BC7F95
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67A → V in AAA74234 (PubMed:7786847).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36128 mRNA. Translation: AAA74234.1.
BT030667 mRNA. Translation: ABS44983.1.
BC105193 mRNA. Translation: AAI05194.1.
RefSeqiNP_776664.1. NM_174239.2.
UniGeneiBt.4732.

Genome annotation databases

EnsembliENSBTAT00000010661; ENSBTAP00000010661; ENSBTAG00000008103.
GeneIDi281615.
KEGGibta:281615.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36128 mRNA. Translation: AAA74234.1.
BT030667 mRNA. Translation: ABS44983.1.
BC105193 mRNA. Translation: AAI05194.1.
RefSeqiNP_776664.1. NM_174239.2.
UniGeneiBt.4732.

3D structure databases

ProteinModelPortaliP48644.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010661.

Proteomic databases

PaxDbiP48644.
PeptideAtlasiP48644.
PRIDEiP48644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010661; ENSBTAP00000010661; ENSBTAG00000008103.
GeneIDi281615.
KEGGibta:281615.

Organism-specific databases

CTDi216.

Phylogenomic databases

eggNOGiKOG2450. Eukaryota.
COG1012. LUCA.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
HOVERGENiHBG000097.
InParanoidiP48644.
KOiK07249.
OMAiEWHSSVS.
OrthoDBiEOG091G05E8.
TreeFamiTF300455.

Enzyme and pathway databases

UniPathwayiUPA00912.
ReactomeiR-BTA-5365859. RA biosynthesis pathway.
R-BTA-70350. Fructose catabolism.
R-BTA-71384. Ethanol oxidation.

Gene expression databases

BgeeiENSBTAG00000008103.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAL1A1_BOVIN
AccessioniPrimary (citable) accession number: P48644
Secondary accession number(s): A7E3P6, Q3MHL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 13, 2007
Last modified: November 30, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.