Reviewed, UniProtKB/Swiss-Prot P48644 (AL1A1_BOVIN)

Names and origin

Protein names

Recommended name:
    Retinal dehydrogenase 1
      Short name=RALDH 1
      Short name=RalDH1
    EC=1.2.1.36
Alternative name(s):
    Aldehyde dehydrogenase family 1 member A1
    Aldehyde dehydrogenase, cytosolic
    ALHDII
    ALDH-E1

Gene names

Name:	ALDH1A1
Synonyms:	ALDH1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid By similarity.
Catalytic activity	Retinal + NAD(+) + H(2)O = retinoate + NADH.
Pathway	Cofactor metabolism; retinol metabolism.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	retinal dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 501	500	Retinal dehydrogenase 1		PRO_0000056413
Regions
	Nucleotide binding	246 – 251	6	NAD By similarity
Sites
	Active site	269	1	Proton acceptor By similarity
	Active site	303	1	Nucleophile By similarity
	Site	170	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
Experimental info
	Sequence conflict	67	1	A → V in AAA74234. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48644-1 [UniParc]. Last modified November 13, 2007. Version 3. Checksum: C9025D7034BC7F95	FASTA	501	54,806
	10 20 30 40 50 60 MSSSAMPDVP APLTNLQFKY TKIFINNEWH SSVSGKKFPV FNPATEEKLC EVEEGDKEDV 70 80 90 100 110 120 DKAVKAARQA FQIGSPWRTM DASERGRLLN KLADLIERDH LLLATMEAMN GGKLFSNAYL 130 140 150 160 170 180 MDLGGCIKTL RYCAGWADKI QGRTIPMDGN FFTYTRSEPV GVCGQIIPWN FPLLMFLWKI 190 200 210 220 230 240 GPALSCGNTV VVKPAEQTPL TALHMGSLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD 250 260 270 280 290 300 KVAFTGSTEV GKLIKEAAGK SNLKRVSLEL GGKSPCIVFA DADLDNAVEF AHQGVFYHQG 310 320 330 340 350 360 QCCIAASRLF VEESIYDEFV RRSVERAKKY VLGNPLTPGV SQGPQIDKEQ YEKILDLIES 370 380 390 400 410 420 GKKEGAKLEC GGGPWGNKGY FIQPTVFSDV TDDMRIAKEE IFGPVQQIMK FKSLDDVIKR 430 440 450 460 470 480 ANNTFYGLSA GIFTNDIDKA ITVSSALQSG TVWVNCYSVV SAQCPFGGFK MSGNGRELGE 490 500 YGFHEYTEVK TVTIKISQKN S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and localization of an aldehyde dehydrogenase to amacrine cells of bovine retina." Saari J.C., Champer R.J., Asson-Batres M.A., Garwin G.G., Huang J., Crabb J.W., Milam A.H. Vis. Neurosci. 12:263-272(1995) [PubMed: 7786847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L36128 mRNA. Translation: AAA74234.1. BT030667 mRNA. Translation: ABS44983.1. BC105193 mRNA. Translation: AAI05194.1.
RefSeq	NP_776664.1.
UniGene	Bt.4732
3D structure databases
HSSP	HSSP built from PDB template 1BXS based on UniProtKB P51977.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000008103. Bos taurus. [Contig view]
GeneID	281615.
KEGG	bta:281615.
Phylogenomic databases
HOVERGEN	P48644.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL1A1_BOVIN

Accession

Primary (citable) accession number: P48644
Secondary accession number(s): A7E3P6, Q3MHL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 13, 2007
Last modified:	November 4, 2008
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)