Reviewed, UniProtKB/Swiss-Prot P48643 (TCPE_HUMAN)

Names and origin

Protein names

Recommended name:
    T-complex protein 1 subunit epsilon
      Short name=TCP-1-epsilon
Alternative name(s):
    CCT-epsilon

Gene names

Name:	CCT5
Synonyms:	CCTE, KIAA0098

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Molecular chaperone; assist the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.
Subunit structure	Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG.
Subcellular location	Cytoplasm.
Involvement in disease	Defects in CCT5 are the cause of autosomal recessive sensory neuropathy with spastic paraplegia [MIM:256840]. The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs.
Sequence similarities	Belongs to the TCP-1 chaperonin family.

Ontologies

Keywords
Cellular component	Cytoplasm
Disease	Disease mutation
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular component	nucleolus Inferred from direct assay. Source: HPA
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
XRN1	Q8IZH2	1	EBI-355710,EBI-372406

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 541	540	T-complex protein 1 subunit epsilon		PRO_0000128346
Amino acid modifications
	Modified residue	2	1	N-acetylalanine
Natural variations
	Natural variant	147	1	H → R in autosomal recessive sensory neuropathy with spastic paraplegia.		VAR_030658

Sequences

	Sequence		Length	Mass (Da)
	P48643-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 164168BB80EF022A	FASTA	541	59,671
	10 20 30 40 50 60 MASMGTLAFD EYGRPFLIIK DQDRKSRLMG LEALKSHIMA AKAVANTMRT SLGPNGLDKM 70 80 90 100 110 120 MVDKDGDVTV TNDGATILSM MDVDHQIAKL MVELSKSQDD EIGDGTTGVV VLAGALLEEA 130 140 150 160 170 180 EQLLDRGIHP IRIADGYEQA ARVAIEHLDK ISDSVLVDIK DTEPLIQTAK TTLGSKVVNS 190 200 210 220 230 240 CHRQMAEIAV NAVLTVADME RRDVDFELIK VEGKVGGRLE DTKLIKGVIV DKDFSHPQMP 250 260 270 280 290 300 KKVEDAKIAI LTCPFEPPKP KTKHKLDVTS VEDYKALQKY EKEKFEEMIQ QIKETGANLA 310 320 330 340 350 360 ICQWGFDDEA NHLLLQNNLP AVRWVGGPEI ELIAIATGGR IVPRFSELTA EKLGFAGLVQ 370 380 390 400 410 420 EISFGTTKDK MLVIEQCKNS RAVTIFIRGG NKMIIEEAKR SLHDALCVIR NLIRDNRVVY 430 440 450 460 470 480 GGGAAEISCA LAVSQEADKC PTLEQYAMRA FADALEVIPM ALSENSGMNP IQTMTEVRAR 490 500 510 520 530 540 QVKEMNPALG IDCLHKGTND MKQQHVIETL IGKKQQISLA TQMVRMILKI DDIRKPGESE E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N. DNA Res. 2:37-43(1995) [PubMed: 7788527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Uterus.
[3]	Bienvenut W.V., Vousden K.H., Lukashchuk N. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-24; 28-35; 50-59; 90-96; 133-170; 184-201; 203-214; 248-261; 264-275; 283-293; 324-340; 345-368; 382-388; 393-399; 401-410; 484-496; 514-525 AND 530-541, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[4]	Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 248-261; 324-340 AND 353-368, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[5]	"A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death." Imai Y., Soda M., Murakami T., Shoji M., Abe K., Takahashi R. J. Biol. Chem. 278:51901-51910(2003) [PubMed: 14532270] [Abstract] Cited for: INTERACTION WITH PACRG.
[6]	"Mutation in the epsilon subunit of the cytosolic chaperonin-containing T-complex peptide-1 (Cct5) gene causes autosomal recessive mutilating sensory neuropathy with spastic paraplegia." Bouhouche A., Benomar A., Bouslam N., Chkili T., Yahyaoui M. J. Med. Genet. 43:441-443(2006) [PubMed: 16399879] [Abstract] Cited for: VARIANT AUTOSOMAL RECESSIVE SENSORY NEUROPATHY WITH SPASTIC PARAPLEGIA ARG-147.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D43950 mRNA. Translation: BAA07894.2. Different initiation. BC006543 mRNA. Translation: AAH06543.1. BC035499 mRNA. Translation: AAH35499.1.
RefSeq	NP_036205.1.
UniGene	Hs.1600
3D structure databases
HSSP	HSSP built from PDB template 1A6D based on UniProtKB P48425.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48643.
PTM databases
PhosphoSite	P48643.
2-D gel databases
SWISS-2DPAGE	P48643.
OGP	P48643.
PHCI-2DPAGE	P48643.
REPRODUCTION-2DPAGE	IPI00010720.
Proteomic databases
PeptideAtlas	P48643.
Genome annotation databases
Ensembl	ENSG00000150753. Homo sapiens. [Contig view]
GeneID	22948.
KEGG	hsa:22948.
Organism-specific databases
H-InvDB	HIX0004744.
HGNC	HGNC:1618. CCT5.
HPA	CAB006271. HPA002238. HPA005958.
MIM	256840. phenotype. 610150. gene.
Orphanet	2683. Neuropathy sensory - spastic paraplegia.
PharmGKB	PA26182.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P48643.
HOVERGEN	P48643.
Gene expression databases
ArrayExpress	P48643.
CleanEx	HS_CCT5.
GermOnline	ENSG00000150753. Homo sapiens.
Family and domain databases
InterPro	IPR012718. Chap_CCT_epsi. IPR002194. Chaperonin_TCP-1_CS. IPR001844. Chaprnin_Cpn60. IPR002423. Cpn60/TCP-1. [Graphical view]
PANTHER	PTHR11353:SF25. Chap_CCT_epsi. 1 hit. PTHR11353. Cpn60/TCP-1. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00298. CHAPERONIN60. PR00304. TCOMPLEXTCP1.
TIGRFAMs	TIGR02343. chap_CCT_epsi. 1 hit.
PROSITE	PS00750. TCP1_1. 1 hit. PS00751. TCP1_2. 1 hit. PS00995. TCP1_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P48643.
NextBio	43711.
SOURCE	Search...

Entry information

Entry name

TCPE_HUMAN

Accession

Primary (citable) accession number: P48643

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)