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P48642

- GSHRC_ORYSJ

UniProt

P48642 - GSHRC_ORYSJ

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Protein

Glutathione reductase, cytosolic

Gene

GRC2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Maintains high levels of reduced glutathione in the cytosol.

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei231 – 2311NADPBy similarity
Binding sitei237 – 2371NADPBy similarity
Active sitei469 – 4691Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 7010FADBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: EnsemblPlants/Gramene
  2. glutathione-disulfide reductase activity Source: UniProtKB-EC
  3. NADP binding Source: EnsemblPlants/Gramene

GO - Biological processi

  1. cell redox homeostasis Source: EnsemblPlants/Gramene
  2. glutathione metabolic process Source: EnsemblPlants/Gramene
  3. toxin catabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.8.1.7. 4460.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase, cytosolic (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Gene namesi
Name:GRC2
Synonyms:RGRC2
Ordered Locus Names:Os02g0813500, LOC_Os02g56850
ORF Names:OJ1293_E04.4-1, OSJNBa0053L11.26-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiP48642.

Subcellular locationi

GO - Cellular componenti

  1. peroxisome Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Glutathione reductase, cytosolicPRO_0000067963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 75Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP48642.
PRIDEiP48642.

Expressioni

Gene expression databases

ExpressionAtlasiP48642. baseline.

Structurei

3D structure databases

ProteinModelPortaliP48642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG1249.
HOGENOMiHOG000276712.
InParanoidiP48642.
KOiK00383.
OMAiDAKNYGW.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01424. gluta_reduc_2. 1 hit.
PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48642-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARKMLKDEE VEVAVTDGGS YDYDLFVIGA GSGGVRGSRT SASFGAKVAI
60 70 80 90 100
CELPFHPISS DWQGGHGGTC VIRGCVPKKI LVYGSSFRGE FEDAKNFGWE
110 120 130 140 150
INGDINFNWK RLLENKTQEI VRLNGVYQRI LGNSGVTMIE GAGSLVDAHT
160 170 180 190 200
VEVTKPDGSK QRYTAKHILI ATGSRAQRVN IPGKELAITS DEALSLEELP
210 220 230 240 250
KRAVILGGGY IAVEFASIWK GMGAHVDLFY RKELPLRGFD DEMRTVVASN
260 270 280 290 300
LEGRGIRLHP GTNLSELSKT ADGIKVVTDK GEEIIADVVL FATGRTPNSQ
310 320 330 340 350
RLNLEAAGVE VDNIGAIKVD DYSRTSVPNI WAVGDVTNRI NLTPVALMEA
360 370 380 390 400
TCFSKTVFGG QPTKPDYRDV PCAVFSIPPL SVVGLSEQQA LEEAKSDVLV
410 420 430 440 450
YTSSFNPMKN SISKRQEKTV MKLVVDSETD KVLGASMCGP DAPEIIQGMA
460 470 480 490
VALKCGATKA TFDSTVGIHP SAAEEFVTMR TLTRRVSPSS KPKTNL
Length:496
Mass (Da):53,507
Last modified:September 26, 2001 - v2
Checksum:iA27FEE5D847F97F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311G → V in BAA11214. (PubMed:10050312)Curated
Sequence conflicti48 – 481V → F in BAA11214. (PubMed:10050312)Curated
Sequence conflicti97 – 971F → L in BAA11214. (PubMed:10050312)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78136 mRNA. Translation: BAA11214.1.
D85751 mRNA. Translation: BAA36283.1.
AB009592 Genomic DNA. Translation: BAA37092.1.
AP004120 Genomic DNA. Translation: BAD21653.1.
AP005691 Genomic DNA. Translation: BAD22392.1.
AY136760 Genomic DNA. Translation: AAN15933.1.
AY136761 Genomic DNA. Translation: AAN15934.1.
AY136762 Genomic DNA. Translation: AAN15935.1.
AY136763 Genomic DNA. Translation: AAN15936.1.
AY136764 Genomic DNA. Translation: AAN15937.1.
AY136765 Genomic DNA. Translation: AAN15938.1.
AY136766 Genomic DNA. Translation: AAN15939.1.
PIRiT03766.
RefSeqiNP_001048485.1. NM_001055020.1.
UniGeneiOs.4154.

Genome annotation databases

EnsemblPlantsiOS02T0813500-01; OS02T0813500-01; OS02G0813500.
GeneIDi4331112.
KEGGiosa:4331112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78136 mRNA. Translation: BAA11214.1 .
D85751 mRNA. Translation: BAA36283.1 .
AB009592 Genomic DNA. Translation: BAA37092.1 .
AP004120 Genomic DNA. Translation: BAD21653.1 .
AP005691 Genomic DNA. Translation: BAD22392.1 .
AY136760 Genomic DNA. Translation: AAN15933.1 .
AY136761 Genomic DNA. Translation: AAN15934.1 .
AY136762 Genomic DNA. Translation: AAN15935.1 .
AY136763 Genomic DNA. Translation: AAN15936.1 .
AY136764 Genomic DNA. Translation: AAN15937.1 .
AY136765 Genomic DNA. Translation: AAN15938.1 .
AY136766 Genomic DNA. Translation: AAN15939.1 .
PIRi T03766.
RefSeqi NP_001048485.1. NM_001055020.1.
UniGenei Os.4154.

3D structure databases

ProteinModelPortali P48642.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P48642.
PRIDEi P48642.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS02T0813500-01 ; OS02T0813500-01 ; OS02G0813500 .
GeneIDi 4331112.
KEGGi osa:4331112.

Organism-specific databases

Gramenei P48642.

Phylogenomic databases

eggNOGi COG1249.
HOGENOMi HOG000276712.
InParanoidi P48642.
KOi K00383.
OMAi DAKNYGW.

Enzyme and pathway databases

BRENDAi 1.8.1.7. 4460.

Gene expression databases

ExpressionAtlasi P48642. baseline.

Family and domain databases

Gene3Di 3.30.390.30. 1 hit.
InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view ]
Pfami PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF55424. SSF55424. 1 hit.
TIGRFAMsi TIGR01424. gluta_reduc_2. 1 hit.
PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)."
    Kaminaka H., Morita S., Nakajima M., Masumura T., Tanaka K.
    Plant Cell Physiol. 39:1269-1280(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Molecular evidence on the origin and evolution of glutinous rice."
    Olsen K.M., Purugganan M.D.
    Genetics 162:941-950(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-413.

Entry informationi

Entry nameiGSHRC_ORYSJ
AccessioniPrimary (citable) accession number: P48642
Secondary accession number(s): Q6K3E8, Q8GRV3, Q9ZNS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 26, 2001
Last modified: October 29, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is a redox-active disulfide bond.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3