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P48642

- GSHRC_ORYSJ

UniProt

P48642 - GSHRC_ORYSJ

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Protein

Glutathione reductase, cytosolic

Gene
GRC2, RGRC2, Os02g0813500, LOC_Os02g56850, OJ1293_E04.4-1, OSJNBa0053L11.26-1
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Maintains high levels of reduced glutathione in the cytosol.

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

Binds 1 FAD per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei231 – 2311NADP By similarity
Binding sitei237 – 2371NADP By similarity
Active sitei469 – 4691Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 7010FAD By similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. glutathione-disulfide reductase activity Source: UniProtKB-EC
  3. NADP binding Source: InterPro

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. glutathione metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.8.1.7. 4460.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase, cytosolic (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Gene namesi
Name:GRC2
Synonyms:RGRC2
Ordered Locus Names:Os02g0813500, LOC_Os02g56850
ORF Names:OJ1293_E04.4-1, OSJNBa0053L11.26-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiP48642.

Subcellular locationi

GO - Cellular componenti

  1. peroxisome Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Glutathione reductase, cytosolicPRO_0000067963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 75Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP48642.
PRIDEiP48642.

Structurei

3D structure databases

ProteinModelPortaliP48642.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG1249.
HOGENOMiHOG000276712.
KOiK00383.
OMAiDAKNYGW.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01424. gluta_reduc_2. 1 hit.
PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48642-1 [UniParc]FASTAAdd to Basket

« Hide

MARKMLKDEE VEVAVTDGGS YDYDLFVIGA GSGGVRGSRT SASFGAKVAI    50
CELPFHPISS DWQGGHGGTC VIRGCVPKKI LVYGSSFRGE FEDAKNFGWE 100
INGDINFNWK RLLENKTQEI VRLNGVYQRI LGNSGVTMIE GAGSLVDAHT 150
VEVTKPDGSK QRYTAKHILI ATGSRAQRVN IPGKELAITS DEALSLEELP 200
KRAVILGGGY IAVEFASIWK GMGAHVDLFY RKELPLRGFD DEMRTVVASN 250
LEGRGIRLHP GTNLSELSKT ADGIKVVTDK GEEIIADVVL FATGRTPNSQ 300
RLNLEAAGVE VDNIGAIKVD DYSRTSVPNI WAVGDVTNRI NLTPVALMEA 350
TCFSKTVFGG QPTKPDYRDV PCAVFSIPPL SVVGLSEQQA LEEAKSDVLV 400
YTSSFNPMKN SISKRQEKTV MKLVVDSETD KVLGASMCGP DAPEIIQGMA 450
VALKCGATKA TFDSTVGIHP SAAEEFVTMR TLTRRVSPSS KPKTNL 496
Length:496
Mass (Da):53,507
Last modified:September 26, 2001 - v2
Checksum:iA27FEE5D847F97F6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311G → V in BAA11214. 1 Publication
Sequence conflicti48 – 481V → F in BAA11214. 1 Publication
Sequence conflicti97 – 971F → L in BAA11214. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78136 mRNA. Translation: BAA11214.1.
D85751 mRNA. Translation: BAA36283.1.
AB009592 Genomic DNA. Translation: BAA37092.1.
AP004120 Genomic DNA. Translation: BAD21653.1.
AP005691 Genomic DNA. Translation: BAD22392.1.
AY136760 Genomic DNA. Translation: AAN15933.1.
AY136761 Genomic DNA. Translation: AAN15934.1.
AY136762 Genomic DNA. Translation: AAN15935.1.
AY136763 Genomic DNA. Translation: AAN15936.1.
AY136764 Genomic DNA. Translation: AAN15937.1.
AY136765 Genomic DNA. Translation: AAN15938.1.
AY136766 Genomic DNA. Translation: AAN15939.1.
PIRiT03766.
RefSeqiNP_001048485.1. NM_001055020.1.
UniGeneiOs.4154.

Genome annotation databases

EnsemblPlantsiOS02T0813500-01; OS02T0813500-01; OS02G0813500.
GeneIDi4331112.
KEGGiosa:4331112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78136 mRNA. Translation: BAA11214.1 .
D85751 mRNA. Translation: BAA36283.1 .
AB009592 Genomic DNA. Translation: BAA37092.1 .
AP004120 Genomic DNA. Translation: BAD21653.1 .
AP005691 Genomic DNA. Translation: BAD22392.1 .
AY136760 Genomic DNA. Translation: AAN15933.1 .
AY136761 Genomic DNA. Translation: AAN15934.1 .
AY136762 Genomic DNA. Translation: AAN15935.1 .
AY136763 Genomic DNA. Translation: AAN15936.1 .
AY136764 Genomic DNA. Translation: AAN15937.1 .
AY136765 Genomic DNA. Translation: AAN15938.1 .
AY136766 Genomic DNA. Translation: AAN15939.1 .
PIRi T03766.
RefSeqi NP_001048485.1. NM_001055020.1.
UniGenei Os.4154.

3D structure databases

ProteinModelPortali P48642.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P48642.
PRIDEi P48642.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS02T0813500-01 ; OS02T0813500-01 ; OS02G0813500 .
GeneIDi 4331112.
KEGGi osa:4331112.

Organism-specific databases

Gramenei P48642.

Phylogenomic databases

eggNOGi COG1249.
HOGENOMi HOG000276712.
KOi K00383.
OMAi DAKNYGW.

Enzyme and pathway databases

BRENDAi 1.8.1.7. 4460.

Family and domain databases

Gene3Di 3.30.390.30. 1 hit.
InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view ]
Pfami PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF55424. SSF55424. 1 hit.
TIGRFAMsi TIGR01424. gluta_reduc_2. 1 hit.
PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)."
    Kaminaka H., Morita S., Nakajima M., Masumura T., Tanaka K.
    Plant Cell Physiol. 39:1269-1280(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Molecular evidence on the origin and evolution of glutinous rice."
    Olsen K.M., Purugganan M.D.
    Genetics 162:941-950(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-413.

Entry informationi

Entry nameiGSHRC_ORYSJ
AccessioniPrimary (citable) accession number: P48642
Secondary accession number(s): Q6K3E8, Q8GRV3, Q9ZNS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 26, 2001
Last modified: July 9, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is a redox-active disulfide bond.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi