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P48642 (GSHRC_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione reductase, cytosolic
Short name=GR
Short name=GRase
EC=1.8.1.7
Gene names
Name:GRC2
Synonyms:RGRC2
Ordered Locus Names:Os02g0813500, LOC_Os02g56850
ORF Names:OJ1293_E04.4-1, OSJNBa0053L11.26-1
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the cytosol.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Cytoplasm.

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Glutathione reductase, cytosolic
PRO_0000067963

Regions

Nucleotide binding61 – 7010FAD By similarity

Sites

Active site4691Proton acceptor By similarity
Binding site2311NADP By similarity
Binding site2371NADP By similarity

Amino acid modifications

Disulfide bond70 ↔ 75Redox-active By similarity

Experimental info

Sequence conflict311G → V in BAA11214. Ref.1
Sequence conflict481V → F in BAA11214. Ref.1
Sequence conflict971F → L in BAA11214. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48642 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: A27FEE5D847F97F6

FASTA49653,507
        10         20         30         40         50         60 
MARKMLKDEE VEVAVTDGGS YDYDLFVIGA GSGGVRGSRT SASFGAKVAI CELPFHPISS 

        70         80         90        100        110        120 
DWQGGHGGTC VIRGCVPKKI LVYGSSFRGE FEDAKNFGWE INGDINFNWK RLLENKTQEI 

       130        140        150        160        170        180 
VRLNGVYQRI LGNSGVTMIE GAGSLVDAHT VEVTKPDGSK QRYTAKHILI ATGSRAQRVN 

       190        200        210        220        230        240 
IPGKELAITS DEALSLEELP KRAVILGGGY IAVEFASIWK GMGAHVDLFY RKELPLRGFD 

       250        260        270        280        290        300 
DEMRTVVASN LEGRGIRLHP GTNLSELSKT ADGIKVVTDK GEEIIADVVL FATGRTPNSQ 

       310        320        330        340        350        360 
RLNLEAAGVE VDNIGAIKVD DYSRTSVPNI WAVGDVTNRI NLTPVALMEA TCFSKTVFGG 

       370        380        390        400        410        420 
QPTKPDYRDV PCAVFSIPPL SVVGLSEQQA LEEAKSDVLV YTSSFNPMKN SISKRQEKTV 

       430        440        450        460        470        480 
MKLVVDSETD KVLGASMCGP DAPEIIQGMA VALKCGATKA TFDSTVGIHP SAAEEFVTMR 

       490 
TLTRRVSPSS KPKTNL

« Hide

References

« Hide 'large scale' references
[1]"Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)."
Kaminaka H., Morita S., Nakajima M., Masumura T., Tanaka K.
Plant Cell Physiol. 39:1269-1280(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Molecular evidence on the origin and evolution of glutinous rice."
Olsen K.M., Purugganan M.D.
Genetics 162:941-950(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-413.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D78136 mRNA. Translation: BAA11214.1.
D85751 mRNA. Translation: BAA36283.1.
AB009592 Genomic DNA. Translation: BAA37092.1.
AP004120 Genomic DNA. Translation: BAD21653.1.
AP005691 Genomic DNA. Translation: BAD22392.1.
AY136760 Genomic DNA. Translation: AAN15933.1.
AY136761 Genomic DNA. Translation: AAN15934.1.
AY136762 Genomic DNA. Translation: AAN15935.1.
AY136763 Genomic DNA. Translation: AAN15936.1.
AY136764 Genomic DNA. Translation: AAN15937.1.
AY136765 Genomic DNA. Translation: AAN15938.1.
AY136766 Genomic DNA. Translation: AAN15939.1.
PIRT03766.
RefSeqNP_001048485.1. NM_001055020.1.
UniGeneOs.4154.

3D structure databases

ProteinModelPortalP48642.
ModBaseSearch...

Proteomic databases

PaxDbP48642.
PRIDEP48642.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsLOC_Os02g56850.1; LOC_Os02g56850.1; LOC_Os02g56850.
LOC_Os02g56850.2; LOC_Os02g56850.2; LOC_Os02g56850.
LOC_Os02g56850.3; LOC_Os02g56850.3; LOC_Os02g56850.
GeneID4331112.
KEGGdosa:Os02t0813500-01.
osa:4331112.

Organism-specific databases

GrameneP48642.

Phylogenomic databases

eggNOGCOG1249.
HOGENOMHOG000276712.
KOK00383.
OMADEANATH.
ProtClustDBPLN02507.

Enzyme and pathway databases

BRENDA1.8.1.7. 4460.

Gene expression databases

ArrayExpressP48642.

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
TIGRFAMsTIGR01424. gluta_reduc_2. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHRC_ORYSJ
AccessionPrimary (citable) accession number: P48642
Secondary accession number(s): Q6K3E8, Q8GRV3, Q9ZNS8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 26, 2001
Last modified: April 3, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

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