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P48642

- GSHRC_ORYSJ

UniProt

P48642 - GSHRC_ORYSJ

Protein

Glutathione reductase, cytosolic

Gene

GRC2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Maintains high levels of reduced glutathione in the cytosol.

    Catalytic activityi

    2 glutathione + NADP+ = glutathione disulfide + NADPH.

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei231 – 2311NADPBy similarity
    Binding sitei237 – 2371NADPBy similarity
    Active sitei469 – 4691Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi61 – 7010FADBy similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. glutathione-disulfide reductase activity Source: UniProtKB-EC
    3. NADP binding Source: InterPro

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. glutathione metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BRENDAi1.8.1.7. 4460.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione reductase, cytosolic (EC:1.8.1.7)
    Short name:
    GR
    Short name:
    GRase
    Gene namesi
    Name:GRC2
    Synonyms:RGRC2
    Ordered Locus Names:Os02g0813500, LOC_Os02g56850
    ORF Names:OJ1293_E04.4-1, OSJNBa0053L11.26-1
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 2

    Organism-specific databases

    GrameneiP48642.

    Subcellular locationi

    GO - Cellular componenti

    1. peroxisome Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496Glutathione reductase, cytosolicPRO_0000067963Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi70 ↔ 75Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP48642.
    PRIDEiP48642.

    Structurei

    3D structure databases

    ProteinModelPortaliP48642.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG1249.
    HOGENOMiHOG000276712.
    KOiK00383.
    OMAiDAKNYGW.

    Family and domain databases

    Gene3Di3.30.390.30. 1 hit.
    InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR006324. Glut-diS_reduct.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR012999. Pyr_OxRdtase_I_AS.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view]
    PfamiPF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view]
    PRINTSiPR00368. FADPNR.
    SUPFAMiSSF55424. SSF55424. 1 hit.
    TIGRFAMsiTIGR01424. gluta_reduc_2. 1 hit.
    PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48642-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARKMLKDEE VEVAVTDGGS YDYDLFVIGA GSGGVRGSRT SASFGAKVAI    50
    CELPFHPISS DWQGGHGGTC VIRGCVPKKI LVYGSSFRGE FEDAKNFGWE 100
    INGDINFNWK RLLENKTQEI VRLNGVYQRI LGNSGVTMIE GAGSLVDAHT 150
    VEVTKPDGSK QRYTAKHILI ATGSRAQRVN IPGKELAITS DEALSLEELP 200
    KRAVILGGGY IAVEFASIWK GMGAHVDLFY RKELPLRGFD DEMRTVVASN 250
    LEGRGIRLHP GTNLSELSKT ADGIKVVTDK GEEIIADVVL FATGRTPNSQ 300
    RLNLEAAGVE VDNIGAIKVD DYSRTSVPNI WAVGDVTNRI NLTPVALMEA 350
    TCFSKTVFGG QPTKPDYRDV PCAVFSIPPL SVVGLSEQQA LEEAKSDVLV 400
    YTSSFNPMKN SISKRQEKTV MKLVVDSETD KVLGASMCGP DAPEIIQGMA 450
    VALKCGATKA TFDSTVGIHP SAAEEFVTMR TLTRRVSPSS KPKTNL 496
    Length:496
    Mass (Da):53,507
    Last modified:September 26, 2001 - v2
    Checksum:iA27FEE5D847F97F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 311G → V in BAA11214. (PubMed:10050312)Curated
    Sequence conflicti48 – 481V → F in BAA11214. (PubMed:10050312)Curated
    Sequence conflicti97 – 971F → L in BAA11214. (PubMed:10050312)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78136 mRNA. Translation: BAA11214.1.
    D85751 mRNA. Translation: BAA36283.1.
    AB009592 Genomic DNA. Translation: BAA37092.1.
    AP004120 Genomic DNA. Translation: BAD21653.1.
    AP005691 Genomic DNA. Translation: BAD22392.1.
    AY136760 Genomic DNA. Translation: AAN15933.1.
    AY136761 Genomic DNA. Translation: AAN15934.1.
    AY136762 Genomic DNA. Translation: AAN15935.1.
    AY136763 Genomic DNA. Translation: AAN15936.1.
    AY136764 Genomic DNA. Translation: AAN15937.1.
    AY136765 Genomic DNA. Translation: AAN15938.1.
    AY136766 Genomic DNA. Translation: AAN15939.1.
    PIRiT03766.
    RefSeqiNP_001048485.1. NM_001055020.1.
    UniGeneiOs.4154.

    Genome annotation databases

    EnsemblPlantsiOS02T0813500-01; OS02T0813500-01; OS02G0813500.
    GeneIDi4331112.
    KEGGiosa:4331112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78136 mRNA. Translation: BAA11214.1 .
    D85751 mRNA. Translation: BAA36283.1 .
    AB009592 Genomic DNA. Translation: BAA37092.1 .
    AP004120 Genomic DNA. Translation: BAD21653.1 .
    AP005691 Genomic DNA. Translation: BAD22392.1 .
    AY136760 Genomic DNA. Translation: AAN15933.1 .
    AY136761 Genomic DNA. Translation: AAN15934.1 .
    AY136762 Genomic DNA. Translation: AAN15935.1 .
    AY136763 Genomic DNA. Translation: AAN15936.1 .
    AY136764 Genomic DNA. Translation: AAN15937.1 .
    AY136765 Genomic DNA. Translation: AAN15938.1 .
    AY136766 Genomic DNA. Translation: AAN15939.1 .
    PIRi T03766.
    RefSeqi NP_001048485.1. NM_001055020.1.
    UniGenei Os.4154.

    3D structure databases

    ProteinModelPortali P48642.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P48642.
    PRIDEi P48642.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS02T0813500-01 ; OS02T0813500-01 ; OS02G0813500 .
    GeneIDi 4331112.
    KEGGi osa:4331112.

    Organism-specific databases

    Gramenei P48642.

    Phylogenomic databases

    eggNOGi COG1249.
    HOGENOMi HOG000276712.
    KOi K00383.
    OMAi DAKNYGW.

    Enzyme and pathway databases

    BRENDAi 1.8.1.7. 4460.

    Family and domain databases

    Gene3Di 3.30.390.30. 1 hit.
    InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR006324. Glut-diS_reduct.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR012999. Pyr_OxRdtase_I_AS.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view ]
    PRINTSi PR00368. FADPNR.
    SUPFAMi SSF55424. SSF55424. 1 hit.
    TIGRFAMsi TIGR01424. gluta_reduc_2. 1 hit.
    PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)."
      Kaminaka H., Morita S., Nakajima M., Masumura T., Tanaka K.
      Plant Cell Physiol. 39:1269-1280(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "Molecular evidence on the origin and evolution of glutinous rice."
      Olsen K.M., Purugganan M.D.
      Genetics 162:941-950(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-413.

    Entry informationi

    Entry nameiGSHRC_ORYSJ
    AccessioniPrimary (citable) accession number: P48642
    Secondary accession number(s): Q6K3E8, Q8GRV3, Q9ZNS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is a redox-active disulfide bond.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3