Reviewed, UniProtKB/Swiss-Prot P48642 (GSHRC_ORYSJ)

Names and origin

Protein names

Recommended name:
    Glutathione reductase, cytosolic
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:	GRC2
Synonyms:	RGRC2
Ordered Locus Names:	Os02g0813500, LOC_Os02g56850
ORF Names:	OJ1293_E04.4-1, OSJNBa0053L11.26-1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol.
Catalytic activity	2 glutathione + NADP(+) = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Glutathione reductase, cytosolic		PRO_0000067963
Regions
	Nucleotide binding	61 – 70	10	FAD By similarity
Sites
	Active site	469	1	Proton acceptor By similarity
	Binding site	231	1	NADP By similarity
	Binding site	237	1	NADP By similarity
Amino acid modifications
	Disulfide bond	70 ↔ 75		Redox-active By similarity
Experimental info
	Sequence conflict	31	1	G → V in BAA11214. Ref.1
	Sequence conflict	48	1	V → F in BAA11214. Ref.1
	Sequence conflict	97	1	F → L in BAA11214. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48642-1 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: A27FEE5D847F97F6	FASTA	496	53,507
	10 20 30 40 50 60 MARKMLKDEE VEVAVTDGGS YDYDLFVIGA GSGGVRGSRT SASFGAKVAI CELPFHPISS 70 80 90 100 110 120 DWQGGHGGTC VIRGCVPKKI LVYGSSFRGE FEDAKNFGWE INGDINFNWK RLLENKTQEI 130 140 150 160 170 180 VRLNGVYQRI LGNSGVTMIE GAGSLVDAHT VEVTKPDGSK QRYTAKHILI ATGSRAQRVN 190 200 210 220 230 240 IPGKELAITS DEALSLEELP KRAVILGGGY IAVEFASIWK GMGAHVDLFY RKELPLRGFD 250 260 270 280 290 300 DEMRTVVASN LEGRGIRLHP GTNLSELSKT ADGIKVVTDK GEEIIADVVL FATGRTPNSQ 310 320 330 340 350 360 RLNLEAAGVE VDNIGAIKVD DYSRTSVPNI WAVGDVTNRI NLTPVALMEA TCFSKTVFGG 370 380 390 400 410 420 QPTKPDYRDV PCAVFSIPPL SVVGLSEQQA LEEAKSDVLV YTSSFNPMKN SISKRQEKTV 430 440 450 460 470 480 MKLVVDSETD KVLGASMCGP DAPEIIQGMA VALKCGATKA TFDSTVGIHP SAAEEFVTMR 490 TLTRRVSPSS KPKTNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene cloning and expression of cytosolic glutathione reductase in rice (Oryza sativa L.)." Kaminaka H., Morita S., Nakajima M., Masumura T., Tanaka K. Plant Cell Physiol. 39:1269-1280(1998) [PubMed: 10050312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Nipponbare.
[2]	"Oryza sativa nipponbare chromosome 2 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Molecular evidence on the origin and evolution of glutinous rice." Olsen K.M., Purugganan M.D. Genetics 162:941-950(2002) [PubMed: 12399401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-413.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D78136 mRNA. Translation: BAA11214.1. D85751 mRNA. Translation: BAA36283.1. AB009592 Genomic DNA. Translation: BAA37092.1. AP004120 Genomic DNA. Translation: BAD21653.1. AP005691 Genomic DNA. Translation: BAD22392.1. AY136760 Genomic DNA. Translation: AAN15933.1. AY136761 Genomic DNA. Translation: AAN15934.1. AY136762 Genomic DNA. Translation: AAN15935.1. AY136763 Genomic DNA. Translation: AAN15936.1. AY136764 Genomic DNA. Translation: AAN15937.1. AY136765 Genomic DNA. Translation: AAN15938.1. AY136766 Genomic DNA. Translation: AAN15939.1.
PIR	T03766.
RefSeq	NP_001048485.1.
UniGene	Os.4154
3D structure databases
HSSP	HSSP built from PDB template 1FEC based on UniProtKB P39040.
ModBase	Search...
Genome annotation databases
GeneID	4331112.
KEGG	osa:4331112.
Organism-specific databases
Gramene	P48642.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut_reduct_pln. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR001864. Trypnth_redctse. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI. PR00470. TRYPANRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRC_ORYSJ

Accession

Primary (citable) accession number: P48642
Secondary accession number(s): Q6K3E8, Q8GRV3, Q9ZNS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	September 26, 2001
Last modified:	November 25, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)