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Protein

Glutathione reductase, cytosolic

Gene

GRC2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Maintains high levels of reduced glutathione in the cytosol.

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei231NADPBy similarity1
Binding sitei237NADPBy similarity1
Active sitei469Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 70FADBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.8.1.7. 4460.
ReactomeiR-OSA-3299685. Detoxification of Reactive Oxygen Species.
R-OSA-499943. Interconversion of nucleotide di- and triphosphates.
R-OSA-5263617. Metabolism of ingested MeSeO2H into MeSeH.
R-OSA-5628897. TP53 Regulates Metabolic Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase, cytosolic (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Gene namesi
Name:GRC2
Synonyms:RGRC2
Ordered Locus Names:Os02g0813500Imported, LOC_Os02g56850Curated
ORF Names:OJ1293_E04.4-1, OsJ_08842Imported, OSJNBa0053L11.26-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000679631 – 496Glutathione reductase, cytosolicAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 75Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP48642.
PRIDEiP48642.

Expressioni

Gene expression databases

ExpressionAtlasiP48642. baseline and differential.
GenevisibleiP48642. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os02g56850.1.

Structurei

3D structure databases

ProteinModelPortaliP48642.
SMRiP48642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0405. Eukaryota.
COG1249. LUCA.
HOGENOMiHOG000276712.
InParanoidiP48642.
KOiK00383.
OMAiGLECACF.
OrthoDBiEOG093608FG.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 1 hit.
InterProiView protein in InterPro
IPR036188. FAD/NAD-bd_sf.
IPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR006324. Glut-diS_reduct.
IPR001100. Pyr_nuc-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR012999. Pyr_OxRdtase_I_AS.
PfamiView protein in Pfam
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
PIRSFiPIRSF000350. Mercury_reductase_MerA. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01424. gluta_reduc_2. 1 hit.
PROSITEiView protein in PROSITE
PS00076. PYRIDINE_REDOX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P48642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKMLKDEE VEVAVTDGGS YDYDLFVIGA GSGGVRGSRT SASFGAKVAI
60 70 80 90 100
CELPFHPISS DWQGGHGGTC VIRGCVPKKI LVYGSSFRGE FEDAKNFGWE
110 120 130 140 150
INGDINFNWK RLLENKTQEI VRLNGVYQRI LGNSGVTMIE GAGSLVDAHT
160 170 180 190 200
VEVTKPDGSK QRYTAKHILI ATGSRAQRVN IPGKELAITS DEALSLEELP
210 220 230 240 250
KRAVILGGGY IAVEFASIWK GMGAHVDLFY RKELPLRGFD DEMRTVVASN
260 270 280 290 300
LEGRGIRLHP GTNLSELSKT ADGIKVVTDK GEEIIADVVL FATGRTPNSQ
310 320 330 340 350
RLNLEAAGVE VDNIGAIKVD DYSRTSVPNI WAVGDVTNRI NLTPVALMEA
360 370 380 390 400
TCFSKTVFGG QPTKPDYRDV PCAVFSIPPL SVVGLSEQQA LEEAKSDVLV
410 420 430 440 450
YTSSFNPMKN SISKRQEKTV MKLVVDSETD KVLGASMCGP DAPEIIQGMA
460 470 480 490
VALKCGATKA TFDSTVGIHP SAAEEFVTMR TLTRRVSPSS KPKTNL
Length:496
Mass (Da):53,507
Last modified:September 26, 2001 - v2
Checksum:iA27FEE5D847F97F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31G → V in BAA11214 (PubMed:10050312).Curated1
Sequence conflicti48V → F in BAA11214 (PubMed:10050312).Curated1
Sequence conflicti97F → L in BAA11214 (PubMed:10050312).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78136 mRNA. Translation: BAA11214.1.
D85751 mRNA. Translation: BAA36283.1.
AB009592 Genomic DNA. Translation: BAA37092.1.
AP004120 Genomic DNA. Translation: BAD21653.1.
AP005691 Genomic DNA. Translation: BAD22392.1.
AP008208 Genomic DNA. Translation: BAF10399.1.
AP014958 Genomic DNA. Translation: BAS81540.1.
CM000139 Genomic DNA. Translation: EAZ25050.1.
AY136760 Genomic DNA. Translation: AAN15933.1.
AY136761 Genomic DNA. Translation: AAN15934.1.
AY136762 Genomic DNA. Translation: AAN15935.1.
AY136763 Genomic DNA. Translation: AAN15936.1.
AY136764 Genomic DNA. Translation: AAN15937.1.
AY136765 Genomic DNA. Translation: AAN15938.1.
AY136766 Genomic DNA. Translation: AAN15939.1.
PIRiT03766.
RefSeqiXP_015626808.1. XM_015771322.1.
XP_015626809.1. XM_015771323.1.
UniGeneiOs.4154.

Genome annotation databases

EnsemblPlantsiOS02T0813500-01; OS02T0813500-01; OS02G0813500.
GeneIDi4331112.
GrameneiOS02T0813500-01; OS02T0813500-01; OS02G0813500.
KEGGiosa:4331112.

Similar proteinsi

Entry informationi

Entry nameiGSHRC_ORYSJ
AccessioniPrimary (citable) accession number: P48642
Secondary accession number(s): A0A0P0VR51
, Q0DWI9, Q6K3E8, Q8GRV3, Q9ZNS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 26, 2001
Last modified: October 25, 2017
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families