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P48641

- GSHRC_ARATH

UniProt

P48641 - GSHRC_ARATH

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Protein
Glutathione reductase, cytosolic
Gene
At3g24170, MUJ8.7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Maintains high levels of reduced glutathione in the cytosol.

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

Binds 1 FAD per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei234 – 2341NADP By similarity
Binding sitei240 – 2401NADP By similarity
Active sitei472 – 4721Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 7310FAD By similarity

GO - Molecular functioni

  1. NADP binding Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. glutathione-disulfide reductase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. glutathione metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciARA:AT3G24170-MONOMER.
ARA:GQT-556-MONOMER.
ARA:GQT-557-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase, cytosolic (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Alternative name(s):
OBP29
Gene namesi
Ordered Locus Names:At3g24170
ORF Names:MUJ8.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G24170.

Subcellular locationi

Cytoplasm Reviewed prediction

GO - Cellular componenti

  1. peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Glutathione reductase, cytosolic
PRO_0000067961Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi73 ↔ 78Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP48641.
PRIDEiP48641.

Expressioni

Gene expression databases

ArrayExpressiP48641.
GenevestigatoriP48641.

Interactioni

Protein-protein interaction databases

DIPiDIP-60756N.

Structurei

3D structure databases

ProteinModelPortaliP48641.
SMRiP48641. Positions 24-489.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG1249.
HOGENOMiHOG000276712.
InParanoidiP48641.
KOiK00383.
OMAiDAKNYGW.
PhylomeDBiP48641.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01424. gluta_reduc_2. 1 hit.
PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48641-1 [UniParc]FASTAAdd to Basket

« Hide

MARKMLVDGE IDKVAADEAN ATHYDFDLFV IGAGSGGVRA ARFSANHGAK    50
VGICELPFHP ISSEEIGGVG GTCVIRGCVP KKILVYGATY GGELEDAKNY 100
GWEINEKVDF TWKKLLQKKT DEILRLNNIY KRLLANAAVK LYEGEGRVVG 150
PNEVEVRQID GTKISYTAKH ILIATGSRAQ KPNIPGHELA ITSDEALSLE 200
EFPKRAIVLG GGYIAVEFAS IWRGMGATVD LFFRKELPLR GFDDEMRALV 250
ARNLEGRGVN LHPQTSLTQL TKTDQGIKVI SSHGEEFVAD VVLFATGRSP 300
NTKRLNLEAV GVELDQAGAV KVDEYSRTNI PSIWAVGDAT NRINLTPVAL 350
MEATCFANTA FGGKPTKAEY SNVACAVFCI PPLAVVGLSE EEAVEQATGD 400
ILVFTSGFNP MKNTISGRQE KTLMKLIVDE KSDKVIGASM CGPDAAEIMQ 450
GIAIALKCGA TKAQFDSTVG IHPSSAEEFV TMRSVTRRIA HKPKPKTNL 499
Length:499
Mass (Da):53,871
Last modified:February 1, 1996 - v1
Checksum:iD6B29E8B5B867186
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621T → A in AAK25938. 1 Publication
Sequence conflicti162 – 1621T → A in AAK64087. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37697 mRNA. Translation: AAB67841.1.
AB028621 Genomic DNA. Translation: BAB01358.1.
CP002686 Genomic DNA. Translation: AEE76865.1.
CP002686 Genomic DNA. Translation: AEE76866.1.
CP002686 Genomic DNA. Translation: AEE76867.1.
AF360228 mRNA. Translation: AAK25938.1.
AY040029 mRNA. Translation: AAK64087.1.
AY140042 mRNA. Translation: AAM98183.1.
AY142628 mRNA. Translation: AAN13086.1.
BT008870 mRNA. Translation: AAP68309.1.
RefSeqiNP_001030756.2. NM_001035679.2.
NP_001118688.1. NM_001125216.1.
NP_189059.1. NM_113322.4.
UniGeneiAt.24980.

Genome annotation databases

EnsemblPlantsiAT3G24170.1; AT3G24170.1; AT3G24170.
AT3G24170.2; AT3G24170.2; AT3G24170.
AT3G24170.3; AT3G24170.3; AT3G24170.
GeneIDi822003.
KEGGiath:AT3G24170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37697 mRNA. Translation: AAB67841.1 .
AB028621 Genomic DNA. Translation: BAB01358.1 .
CP002686 Genomic DNA. Translation: AEE76865.1 .
CP002686 Genomic DNA. Translation: AEE76866.1 .
CP002686 Genomic DNA. Translation: AEE76867.1 .
AF360228 mRNA. Translation: AAK25938.1 .
AY040029 mRNA. Translation: AAK64087.1 .
AY140042 mRNA. Translation: AAM98183.1 .
AY142628 mRNA. Translation: AAN13086.1 .
BT008870 mRNA. Translation: AAP68309.1 .
RefSeqi NP_001030756.2. NM_001035679.2.
NP_001118688.1. NM_001125216.1.
NP_189059.1. NM_113322.4.
UniGenei At.24980.

3D structure databases

ProteinModelPortali P48641.
SMRi P48641. Positions 24-489.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-60756N.

Proteomic databases

PaxDbi P48641.
PRIDEi P48641.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G24170.1 ; AT3G24170.1 ; AT3G24170 .
AT3G24170.2 ; AT3G24170.2 ; AT3G24170 .
AT3G24170.3 ; AT3G24170.3 ; AT3G24170 .
GeneIDi 822003.
KEGGi ath:AT3G24170.

Organism-specific databases

TAIRi AT3G24170.

Phylogenomic databases

eggNOGi COG1249.
HOGENOMi HOG000276712.
InParanoidi P48641.
KOi K00383.
OMAi DAKNYGW.
PhylomeDBi P48641.

Enzyme and pathway databases

BioCyci ARA:AT3G24170-MONOMER.
ARA:GQT-556-MONOMER.
ARA:GQT-557-MONOMER.

Gene expression databases

ArrayExpressi P48641.
Genevestigatori P48641.

Family and domain databases

Gene3Di 3.30.390.30. 1 hit.
InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view ]
Pfami PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF55424. SSF55424. 1 hit.
TIGRFAMsi TIGR01424. gluta_reduc_2. 1 hit.
PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An Arabidopsis cDNA homologous to glutathione reductase."
    Loebler M.
    Plant Gene Register PGR96-053
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiGSHRC_ARATH
AccessioniPrimary (citable) accession number: P48641
Secondary accession number(s): Q2V3T0, Q94BM6, Q9C5I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is a redox-active disulfide bond.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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