P48641 (GSHRC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione reductase, cytosolic
Short name=GR
Short name=GRase
EC=1.8.1.7

Alternative name(s):
OBP29

Gene names

Ordered Locus Names:	At3g24170
ORF Names:	MUJ8.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	peroxisome Inferred from direct assay PubMed 17951448. Source: TAIR
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Glutathione reductase, cytosolic		PRO_0000067961
Regions
	Nucleotide binding	64 – 73	10	FAD By similarity
Sites
	Active site	472	1	Proton acceptor By similarity
	Binding site	234	1	NADP By similarity
	Binding site	240	1	NADP By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 78		Redox-active By similarity
Experimental info
	Sequence conflict	162	1	T → A in AAK25938. Ref.4
	Sequence conflict	162	1	T → A in AAK64087. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P48641 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: D6B29E8B5B867186	FASTA	499	53,871
	10 20 30 40 50 60 MARKMLVDGE IDKVAADEAN ATHYDFDLFV IGAGSGGVRA ARFSANHGAK VGICELPFHP 70 80 90 100 110 120 ISSEEIGGVG GTCVIRGCVP KKILVYGATY GGELEDAKNY GWEINEKVDF TWKKLLQKKT 130 140 150 160 170 180 DEILRLNNIY KRLLANAAVK LYEGEGRVVG PNEVEVRQID GTKISYTAKH ILIATGSRAQ 190 200 210 220 230 240 KPNIPGHELA ITSDEALSLE EFPKRAIVLG GGYIAVEFAS IWRGMGATVD LFFRKELPLR 250 260 270 280 290 300 GFDDEMRALV ARNLEGRGVN LHPQTSLTQL TKTDQGIKVI SSHGEEFVAD VVLFATGRSP 310 320 330 340 350 360 NTKRLNLEAV GVELDQAGAV KVDEYSRTNI PSIWAVGDAT NRINLTPVAL MEATCFANTA 370 380 390 400 410 420 FGGKPTKAEY SNVACAVFCI PPLAVVGLSE EEAVEQATGD ILVFTSGFNP MKNTISGRQE 430 440 450 460 470 480 KTLMKLIVDE KSDKVIGASM CGPDAAEIMQ GIAIALKCGA TKAQFDSTVG IHPSSAEEFV 490 TMRSVTRRIA HKPKPKTNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Arabidopsis cDNA homologous to glutathione reductase." Loebler M. Plant Gene Register PGR96-053 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37697 mRNA. Translation: AAB67841.1. AB028621 Genomic DNA. Translation: BAB01358.1. CP002686 Genomic DNA. Translation: AEE76865.1. CP002686 Genomic DNA. Translation: AEE76866.1. CP002686 Genomic DNA. Translation: AEE76867.1. AF360228 mRNA. Translation: AAK25938.1. AY040029 mRNA. Translation: AAK64087.1. AY140042 mRNA. Translation: AAM98183.1. AY142628 mRNA. Translation: AAN13086.1. BT008870 mRNA. Translation: AAP68309.1.
IPI	IPI00526326.
RefSeq	NP_001030756.2. NM_001035679.2. NP_001118688.1. NM_001125216.1. NP_189059.1. NM_113322.4.
UniGene	At.24980.
3D structure databases
ProteinModelPortal	P48641.
SMR	P48641. Positions 24-489.
ModBase	Search...
Proteomic databases
PaxDb	P48641.
PRIDE	P48641.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G24170.1; AT3G24170.1; AT3G24170. AT3G24170.2; AT3G24170.2; AT3G24170. AT3G24170.3; AT3G24170.3; AT3G24170.
GeneID	822003.
KEGG	ath:AT3G24170.
Organism-specific databases
TAIR	At3g24170.
Phylogenomic databases
eggNOG	COG1249.
HOGENOM	HOG000276712.
InParanoid	P48641.
KO	K00383.
OMA	DEANATH.
PhylomeDB	P48641.
ProtClustDB	PLN02507.
Gene expression databases
ArrayExpress	P48641.
Genevestigator	P48641.
Family and domain databases
Gene3D	3.30.390.30. 1 hit.
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut-diS_reduct. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRC_ARATH

Accession

Primary (citable) accession number: P48641
Secondary accession number(s): Q2V3T0, Q94BM6, Q9C5I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program