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Protein

Glutathione reductase, cytosolic

Gene

At3g24170

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Maintains high levels of reduced glutathione in the cytosol.

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei234NADPBy similarity1
Binding sitei240NADPBy similarity1
Active sitei472Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 73FADBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciARA:AT3G24170-MONOMER
ReactomeiR-ATH-3299685 Detoxification of Reactive Oxygen Species
R-ATH-499943 Interconversion of nucleotide di- and triphosphates
R-ATH-5628897 TP53 Regulates Metabolic Genes

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase, cytosolic (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Alternative name(s):
OBP29
Gene namesi
Ordered Locus Names:At3g24170
ORF Names:MUJ8.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G24170
TAIRilocus:2093691 AT3G24170

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000679611 – 499Glutathione reductase, cytosolicAdd BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 78Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP48641
PRIDEiP48641

Expressioni

Gene expression databases

ExpressionAtlasiP48641 differential
GenevisibleiP48641 AT

Interactioni

Protein-protein interaction databases

DIPiDIP-60756N
STRINGi3702.AT3G24170.1

Structurei

3D structure databases

ProteinModelPortaliP48641
SMRiP48641
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0405 Eukaryota
COG1249 LUCA
HOGENOMiHOG000276712
InParanoidiP48641
KOiK00383
OMAiGLECACF
OrthoDBiEOG093608FG
PhylomeDBiP48641

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR006324 Glut-diS_reduct
IPR001100 Pyr_nuc-diS_OxRdtase
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
PIRSFiPIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
TIGRFAMsiTIGR01424 gluta_reduc_2, 1 hit
PROSITEiView protein in PROSITE
PS00076 PYRIDINE_REDOX_1, 1 hit

Sequencei

Sequence statusi: Complete.

P48641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARKMLVDGE IDKVAADEAN ATHYDFDLFV IGAGSGGVRA ARFSANHGAK
60 70 80 90 100
VGICELPFHP ISSEEIGGVG GTCVIRGCVP KKILVYGATY GGELEDAKNY
110 120 130 140 150
GWEINEKVDF TWKKLLQKKT DEILRLNNIY KRLLANAAVK LYEGEGRVVG
160 170 180 190 200
PNEVEVRQID GTKISYTAKH ILIATGSRAQ KPNIPGHELA ITSDEALSLE
210 220 230 240 250
EFPKRAIVLG GGYIAVEFAS IWRGMGATVD LFFRKELPLR GFDDEMRALV
260 270 280 290 300
ARNLEGRGVN LHPQTSLTQL TKTDQGIKVI SSHGEEFVAD VVLFATGRSP
310 320 330 340 350
NTKRLNLEAV GVELDQAGAV KVDEYSRTNI PSIWAVGDAT NRINLTPVAL
360 370 380 390 400
MEATCFANTA FGGKPTKAEY SNVACAVFCI PPLAVVGLSE EEAVEQATGD
410 420 430 440 450
ILVFTSGFNP MKNTISGRQE KTLMKLIVDE KSDKVIGASM CGPDAAEIMQ
460 470 480 490
GIAIALKCGA TKAQFDSTVG IHPSSAEEFV TMRSVTRRIA HKPKPKTNL
Length:499
Mass (Da):53,871
Last modified:February 1, 1996 - v1
Checksum:iD6B29E8B5B867186
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162T → A in AAK25938 (PubMed:14593172).Curated1
Sequence conflicti162T → A in AAK64087 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37697 mRNA Translation: AAB67841.1
AB028621 Genomic DNA Translation: BAB01358.1
CP002686 Genomic DNA Translation: AEE76865.1
CP002686 Genomic DNA Translation: AEE76866.1
CP002686 Genomic DNA Translation: AEE76867.1
AF360228 mRNA Translation: AAK25938.1
AY040029 mRNA Translation: AAK64087.1
AY140042 mRNA Translation: AAM98183.1
AY142628 mRNA Translation: AAN13086.1
BT008870 mRNA Translation: AAP68309.1
RefSeqiNP_001030756.2, NM_001035679.2
NP_001118688.1, NM_001125216.1
NP_189059.1, NM_113322.5
UniGeneiAt.24980

Genome annotation databases

EnsemblPlantsiAT3G24170.1; AT3G24170.1; AT3G24170
AT3G24170.2; AT3G24170.2; AT3G24170
AT3G24170.3; AT3G24170.3; AT3G24170
GeneIDi822003
GrameneiAT3G24170.1; AT3G24170.1; AT3G24170
AT3G24170.2; AT3G24170.2; AT3G24170
AT3G24170.3; AT3G24170.3; AT3G24170
KEGGiath:AT3G24170

Similar proteinsi

Entry informationi

Entry nameiGSHRC_ARATH
AccessioniPrimary (citable) accession number: P48641
Secondary accession number(s): Q2V3T0, Q94BM6, Q9C5I4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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