Reviewed, UniProtKB/Swiss-Prot P48641 (GSHRC_ARATH)

Names and origin

Protein names

Recommended name:
    Glutathione reductase, cytosolic
      Short name=GRase
      Short name=GR
    EC=1.8.1.7
Alternative name(s):
    OBP29

Gene names

Ordered Locus Names:	At3g24170
ORF Names:	MUJ8.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from direct assay. Source: TAIR
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Glutathione reductase, cytosolic		PRO_0000067961
Regions
	Nucleotide binding	64 – 73	10	FAD By similarity
Sites
	Active site	472	1	Proton acceptor By similarity
	Binding site	234	1	NADP By similarity
	Binding site	240	1	NADP By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 78		Redox-active By similarity
Experimental info
	Sequence conflict	162	1	T → A in AAK25938. Ref.3
	Sequence conflict	162	1	T → A in AAK64087. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P48641-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: D6B29E8B5B867186	FASTA	499	53,871
	10 20 30 40 50 60 MARKMLVDGE IDKVAADEAN ATHYDFDLFV IGAGSGGVRA ARFSANHGAK VGICELPFHP 70 80 90 100 110 120 ISSEEIGGVG GTCVIRGCVP KKILVYGATY GGELEDAKNY GWEINEKVDF TWKKLLQKKT 130 140 150 160 170 180 DEILRLNNIY KRLLANAAVK LYEGEGRVVG PNEVEVRQID GTKISYTAKH ILIATGSRAQ 190 200 210 220 230 240 KPNIPGHELA ITSDEALSLE EFPKRAIVLG GGYIAVEFAS IWRGMGATVD LFFRKELPLR 250 260 270 280 290 300 GFDDEMRALV ARNLEGRGVN LHPQTSLTQL TKTDQGIKVI SSHGEEFVAD VVLFATGRSP 310 320 330 340 350 360 NTKRLNLEAV GVELDQAGAV KVDEYSRTNI PSIWAVGDAT NRINLTPVAL MEATCFANTA 370 380 390 400 410 420 FGGKPTKAEY SNVACAVFCI PPLAVVGLSE EEAVEQATGD ILVFTSGFNP MKNTISGRQE 430 440 450 460 470 480 KTLMKLIVDE KSDKVIGASM CGPDAAEIMQ GIAIALKCGA TKAQFDSTVG IHPSSAEEFV 490 TMRSVTRRIA HKPKPKTNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Arabidopsis cDNA homologous to glutathione reductase." Loebler M. Plant Gene Register PGR96-053 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U37697 mRNA. Translation: AAB67841.1. AB028621 Genomic DNA. Translation: BAB01358.1. AF360228 mRNA. Translation: AAK25938.1. AY040029 mRNA. Translation: AAK64087.1. AY140042 mRNA. Translation: AAM98183.1. AY142628 mRNA. Translation: AAN13086.1. BT008870 mRNA. Translation: AAP68309.1.
IPI	IPI00526326.
RefSeq	NP_001030756.2. NP_001118688.1. NP_189059.1.
UniGene	At.24980
3D structure databases
HSSP	HSSP built from PDB template 1ONF based on UniProtKB Q94655.
ModBase	Search...
Proteomic databases
PRIDE	P48641.
Genome annotation databases
GeneID	822003.
GenomeReviews	Gene locus AT3G24170 in contig BA000014_GR.
KEGG	ath:AT3G24170.
NMPDR	fig|3702.1.peg.14677.
Organism-specific databases
TAIR	At3g24170.
Phylogenomic databases
OMA	P48641. FFRKELP.
Enzyme and pathway databases
BRENDA	1.8.1.7. 302.
Gene expression databases
ArrayExpress	P48641.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut_reduct_pln. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRC_ARATH

Accession

Primary (citable) accession number: P48641
Secondary accession number(s): Q2V3T0, Q94BM6, Q9C5I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)