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P48641 (GSHRC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione reductase, cytosolic

Short name=GR
Short name=GRase
EC=1.8.1.7
Alternative name(s):
OBP29
Gene names
Ordered Locus Names:At3g24170
ORF Names:MUJ8.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the cytosol.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Cytoplasm Potential.

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Glutathione reductase, cytosolic
PRO_0000067961

Regions

Nucleotide binding64 – 7310FAD By similarity

Sites

Active site4721Proton acceptor By similarity
Binding site2341NADP By similarity
Binding site2401NADP By similarity

Amino acid modifications

Disulfide bond73 ↔ 78Redox-active By similarity

Experimental info

Sequence conflict1621T → A in AAK25938. Ref.4
Sequence conflict1621T → A in AAK64087. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P48641 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: D6B29E8B5B867186

FASTA49953,871
        10         20         30         40         50         60 
MARKMLVDGE IDKVAADEAN ATHYDFDLFV IGAGSGGVRA ARFSANHGAK VGICELPFHP 

        70         80         90        100        110        120 
ISSEEIGGVG GTCVIRGCVP KKILVYGATY GGELEDAKNY GWEINEKVDF TWKKLLQKKT 

       130        140        150        160        170        180 
DEILRLNNIY KRLLANAAVK LYEGEGRVVG PNEVEVRQID GTKISYTAKH ILIATGSRAQ 

       190        200        210        220        230        240 
KPNIPGHELA ITSDEALSLE EFPKRAIVLG GGYIAVEFAS IWRGMGATVD LFFRKELPLR 

       250        260        270        280        290        300 
GFDDEMRALV ARNLEGRGVN LHPQTSLTQL TKTDQGIKVI SSHGEEFVAD VVLFATGRSP 

       310        320        330        340        350        360 
NTKRLNLEAV GVELDQAGAV KVDEYSRTNI PSIWAVGDAT NRINLTPVAL MEATCFANTA 

       370        380        390        400        410        420 
FGGKPTKAEY SNVACAVFCI PPLAVVGLSE EEAVEQATGD ILVFTSGFNP MKNTISGRQE 

       430        440        450        460        470        480 
KTLMKLIVDE KSDKVIGASM CGPDAAEIMQ GIAIALKCGA TKAQFDSTVG IHPSSAEEFV 

       490 
TMRSVTRRIA HKPKPKTNL 

« Hide

References

« Hide 'large scale' references
[1]"An Arabidopsis cDNA homologous to glutathione reductase."
Loebler M.
Plant Gene Register PGR96-053
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Leaf.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37697 mRNA. Translation: AAB67841.1.
AB028621 Genomic DNA. Translation: BAB01358.1.
CP002686 Genomic DNA. Translation: AEE76865.1.
CP002686 Genomic DNA. Translation: AEE76866.1.
CP002686 Genomic DNA. Translation: AEE76867.1.
AF360228 mRNA. Translation: AAK25938.1.
AY040029 mRNA. Translation: AAK64087.1.
AY140042 mRNA. Translation: AAM98183.1.
AY142628 mRNA. Translation: AAN13086.1.
BT008870 mRNA. Translation: AAP68309.1.
RefSeqNP_001030756.2. NM_001035679.2.
NP_001118688.1. NM_001125216.1.
NP_189059.1. NM_113322.4.
UniGeneAt.24980.

3D structure databases

ProteinModelPortalP48641.
SMRP48641. Positions 23-484.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP48641.
PRIDEP48641.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G24170.1; AT3G24170.1; AT3G24170.
AT3G24170.2; AT3G24170.2; AT3G24170.
AT3G24170.3; AT3G24170.3; AT3G24170.
GeneID822003.
KEGGath:AT3G24170.

Organism-specific databases

TAIRAT3G24170.

Phylogenomic databases

eggNOGCOG1249.
HOGENOMHOG000276712.
InParanoidP48641.
KOK00383.
OMAWQAFDPE.
PhylomeDBP48641.
ProtClustDBPLN02507.

Enzyme and pathway databases

BioCycARA:AT3G24170-MONOMER.
ARA:GQT-556-MONOMER.
ARA:GQT-557-MONOMER.

Gene expression databases

ArrayExpressP48641.
GenevestigatorP48641.

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01424. gluta_reduc_2. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHRC_ARATH
AccessionPrimary (citable) accession number: P48641
Secondary accession number(s): Q2V3T0, Q94BM6, Q9C5I4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 19, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names