Glutathione reductase, chloroplastic precursor

Preferred order of sections

Names and origin

Protein names

Recommended name:
Glutathione reductase, chloroplastic
Short name=GR
Short name=GRase
EC=1.8.1.7

Gene names

Name:

GR

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the chloroplast.
Catalytic activity	2 glutathione + NADP⁺ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Plastid › chloroplast Potential.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 52

52

Chloroplast Potential

Chain

53 – 544

492

Glutathione reductase, chloroplastic

PRO_0000030282

Regions

Nucleotide binding

105 – 113

9

FAD By similarity

Sites

Active site

507

1

Proton acceptor By similarity

Binding site

269

1

NADP By similarity

Binding site

275

1

NADP By similarity

Amino acid modifications

Disulfide bond

113 ↔ 118

Redox-active By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P48640 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 7A71449EA21CE639
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FASTA

544

58,783

        10         20         30         40         50         60 
MATSLSVSPS LSLNTLFIAK ALPLSRPSFL SLPLPKSLLS LSTRRRTFIV RAESQNGRDP 

        70         80         90        100        110        120 
VPAHYDFDLF TIGAGSGGVR ARRFAANYGA SVAICELPFS TISSETTGVG GTCVIRGCVP 

       130        140        150        160        170        180 
KKLLVYASKF SHEFEESNGF GWRYDSEPKH DWSSFIANKN AELQRLTGIY KNILNNAGVK 

       190        200        210        220        230        240 
LIEGHGKMID PHTVDVNGKL YSAKHILVAV GGRPFIPDIP GKELAIDSDA ALDLPTKPVK 

       250        260        270        280        290        300 
IAIVGGGYIA LEFAGIFNGL KSEVHVFIRQ KKVLRGFDEE IRDFVEEQMS VRGIEFHTEE 

       310        320        330        340        350        360 
SPQAITKSAD GSFSLKTNKG TVDGFSHIMF ATGRRPNTQN LGLESVGVKL AKDGAIEVDE 

       370        380        390        400        410        420 
YSQTSVYSIW AVGDVTNRIN LTPVALMEGG ALVKTLFQDN PTKPDYRAVP SAVFSQPPIG 

       430        440        450        460        470        480 
QVGLTEEQAV QQYGDIDIFT ANFRPLKATL SGLPDRVFMK LVVCAKTNEV LGLHMCGEDA 

       490        500        510        520        530        540 
PEIVQGFAVA LKARLTKADF DATVGIHPSA AEEFVTMRTP TRKIRKSESS EGKSGSQAKA 


AAGV

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References

[1]	"Soybean root nodule cDNA encoding glutathione reductase." Tang X., Webb M.A. Plant Physiol. 104:1081-1082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11632 mRNA. Translation: AAA33962.1.
PIR	T07155.
RefSeq	NP_001238006.1. NM_001251077.1.
UniGene	Gma.32269.
3D structure databases
ProteinModelPortal	P48640.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	547793.
KEGG	gmx:547793.
Gene expression databases
Genevestigator	P48640.
Family and domain databases
Gene3D	3.30.390.30. 1 hit.
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut-diS_reduct. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRP_SOYBN

Accession

Primary (citable) accession number: P48640

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families