Reviewed, UniProtKB/Swiss-Prot P48640 (GSHRP_SOYBN)

Names and origin

Protein names

Recommended name:
    Glutathione reductase, chloroplastic
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:

GR

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the chloroplast.
Catalytic activity	2 glutathione + NADP(+) = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Plastid › chloroplastPotential.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 52	52	Chloroplast Potential
	Chain	53 – 544	492	Glutathione reductase, chloroplastic		PRO_0000030282
Regions
	Nucleotide binding	105 – 113	9	FAD By similarity
Sites
	Active site	507	1	Proton acceptor By similarity
	Binding site	269	1	NADP By similarity
	Binding site	275	1	NADP By similarity
Amino acid modifications
	Disulfide bond	113 ↔ 118		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48640-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7A71449EA21CE639	FASTA	544	58,783
	10 20 30 40 50 60 MATSLSVSPS LSLNTLFIAK ALPLSRPSFL SLPLPKSLLS LSTRRRTFIV RAESQNGRDP 70 80 90 100 110 120 VPAHYDFDLF TIGAGSGGVR ARRFAANYGA SVAICELPFS TISSETTGVG GTCVIRGCVP 130 140 150 160 170 180 KKLLVYASKF SHEFEESNGF GWRYDSEPKH DWSSFIANKN AELQRLTGIY KNILNNAGVK 190 200 210 220 230 240 LIEGHGKMID PHTVDVNGKL YSAKHILVAV GGRPFIPDIP GKELAIDSDA ALDLPTKPVK 250 260 270 280 290 300 IAIVGGGYIA LEFAGIFNGL KSEVHVFIRQ KKVLRGFDEE IRDFVEEQMS VRGIEFHTEE 310 320 330 340 350 360 SPQAITKSAD GSFSLKTNKG TVDGFSHIMF ATGRRPNTQN LGLESVGVKL AKDGAIEVDE 370 380 390 400 410 420 YSQTSVYSIW AVGDVTNRIN LTPVALMEGG ALVKTLFQDN PTKPDYRAVP SAVFSQPPIG 430 440 450 460 470 480 QVGLTEEQAV QQYGDIDIFT ANFRPLKATL SGLPDRVFMK LVVCAKTNEV LGLHMCGEDA 490 500 510 520 530 540 PEIVQGFAVA LKARLTKADF DATVGIHPSA AEEFVTMRTP TRKIRKSESS EGKSGSQAKA AAGV

References

[1]	"Soybean root nodule cDNA encoding glutathione reductase." Tang X., Webb M.A. Plant Physiol. 104:1081-1082(1994) [PubMed: 8165247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Root nodule.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L11632 mRNA. Translation: AAA33962.1.
PIR	T07155.
UniGene	Gma.32269
3D structure databases
HSSP	HSSP built from PDB template 1ONF based on UniProtKB Q94655.
ModBase	Search...
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut_reduct_pln. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR001864. Trypnth_redctse. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI. PR00470. TRYPANRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRP_SOYBN

Accession

Primary (citable) accession number: P48640

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)