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P48639 (GSHR_BURCE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione reductase

Short name=GR
Short name=GRase
EC=1.8.1.7
Gene names
Name:gor
OrganismBurkholderia cepacia (Pseudomonas cepacia)
Taxonomic identifier292 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Pathway

Xenobiotic degradation; (2,4,5-trichlorophenoxy)acetate degradation.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Glutathione reductase
PRO_0000067974

Regions

Nucleotide binding35 – 439FAD By similarity

Sites

Active site4351Proton acceptor By similarity

Amino acid modifications

Disulfide bond43 ↔ 48Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
P48639 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 402FCC6E7A8D6720

FASTA44947,541
        10         20         30         40         50         60 
MQKYDFDLFV IGAGSGGVRA ARIAAGHGAK VAIAEEYRFG GTCVIRGCVP KKLLMYASQY 

        70         80         90        100        110        120 
GQGFEDAAGF GWHSAATSHS WTSLIAAKDA EIARLEGVYQ RLIENANVEI FKGRAQIAGP 

       130        140        150        160        170        180 
NRVTVTGASV SARTILIATG ARPVMPPVAG ANLMITSDDV FDLPVGPPRI AIIGGGYIAC 

       190        200        210        220        230        240 
EFAGIFNGLG RHVVQLHRGS QVLRGFDDEL REHLGDELKK SGIDLRLGVD VVAVERQRGA 

       250        260        270        280        290        300 
LSVQLTTGDA MEVDAVMAAT GRLPNTWGLG LETVDVGLDQ NGAIKVDEYS RTSSPGIYAV 

       310        320        330        340        350        360 
GDVTNRLNLT PVAIHEGHAF ADTVFGGKAL PTEHENVPFA VFSQPQAASV GLSEAQARDR 

       370        380        390        400        410        420 
YSNVEIYGSA FRPMRAALSG RDEKALVKLV VNGSNDRVVG AHIVGADAAE IIQGIAVAIK 

       430        440 
ARATKADFDA TLGVHPTLAE EFVTLRNRR 

« Hide

References

[1]"Sequence analysis of a gene cluster involved in metabolism of 2,4,5-trichlorophenoxyacetic acid by Burkholderia cepacia AC1100."
Daubaras D.L., Hershberger C.D., Kitano K., Chakrabarty A.M.
Appl. Environ. Microbiol. 61:1279-1289(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AC1100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U19883 Genomic DNA. Translation: AAC43334.1.
PIRI40178.

3D structure databases

ProteinModelPortalP48639.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP48639.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00686.

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01424. gluta_reduc_2. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHR_BURCE
AccessionPrimary (citable) accession number: P48639
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 16, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways