P48638 (GSHR_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione reductase
Short name=GR
Short name=GRase
EC=1.8.1.7

Gene names

Name:	gor
Ordered Locus Names:	all4968

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Glutathione reductase		PRO_0000067973
Regions
	Nucleotide binding	34 – 42	9	FAD By similarity
Sites
	Active site	448	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	42 ↔ 47		Redox-active By similarity
Experimental info
	Sequence conflict	234	1	E → Q in CAA61856. Ref.1
	Sequence conflict	340	1	R → L in CAA61856. Ref.1
	Sequence conflict	361	1	V → L in CAA61856. Ref.1
	Sequence conflict	372	1	L → H in CAA61856. Ref.1
	Sequence conflict	381 – 383	3	RTR → AP in CAA61856. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48638 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 29C1F06CE2195888	FASTA	459	49,478
	10 20 30 40 50 60 MTFDYDLFVI GAGSGGLAAS KRAASYGAKV AIAENDLVGG TCVIRGCVPK KLMVYGSHFP 70 80 90 100 110 120 ALFEDAAGYG WQVGKAELNW EHFITSIDKE VRRLSQLHIS FLEKAGVELI SGRATLVDNH 130 140 150 160 170 180 TVEVGERKFT ADKILIAVGG RPIKPELPGM EYGITSNEIF HLKTQPKHIA IIGSGYIGTE 190 200 210 220 230 240 FAGIMRGLGS QVTQITRGDK ILKGFDEDIR TEIQEGMTNH GIRIIPKNVV TAIEQVPEGL 250 260 270 280 290 300 KISLSGEDQE PIIADVFLVA TGRVPNVDGL GLENAGVDVV DSSIEGPGYS TMNAIAVNEY 310 320 330 340 350 360 SQTSQPNIYA VGDVTDRLNL TPVAIGEGRA FADSEFGNNR REFSHETIAT AVFSNPQAST 370 380 390 400 410 420 VGLTEAEARA KLGDDAVTIY RTRFRPMYHS FTGKQERIMM KLVVDTKTDK VLGAHMVGEN 430 440 450 AAEIIQGVAI AVKMGATKKD FDATVGIHPS SAEEFVTMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and regulation of the glutathione reductase gene from the cyanobacterium Anabaena PCC 7120." Jiang F., Hellman U., Sroga G.E., Bergman B., Mannervik B. J. Biol. Chem. 270:22882-22889(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89712 Genomic DNA. Translation: CAA61856.1. BA000019 Genomic DNA. Translation: BAB76667.1.
PIR	AH2426. I39477.
RefSeq	NP_489008.1. NC_003272.1.
3D structure databases
ProteinModelPortal	P48638.
ModBase	Search...
Protein-protein interaction databases
STRING	103690.all4968.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB76667; BAB76667; BAB76667.
GeneID	1108569.
KEGG	ana:all4968.
PATRIC	22780609. VBINosSp37423_5746.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1249.
HOGENOM	HOG000276712.
KO	K00383.
OMA	DEKTLMK.
ProtClustDB	PRK06116.
Family and domain databases
Gene3D	3.30.390.30. 1 hit.
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut-diS_reduct. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHR_NOSS1

Accession

Primary (citable) accession number: P48638

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program