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P48638 (GSHR_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione reductase

Short name=GR
Short name=GRase
EC=1.8.1.7
Gene names
Name:gor
Ordered Locus Names:all4968
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the cytosol.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainRedox-active center
   LigandFAD
Flavoprotein
NADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcell redox homeostasis

Inferred from electronic annotation. Source: InterPro

glutathione metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

flavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

glutathione-disulfide reductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 459459Glutathione reductase
PRO_0000067973

Regions

Nucleotide binding34 – 429FAD By similarity

Sites

Active site4481Proton acceptor By similarity

Amino acid modifications

Disulfide bond42 ↔ 47Redox-active By similarity

Experimental info

Sequence conflict2341E → Q in CAA61856. Ref.1
Sequence conflict3401R → L in CAA61856. Ref.1
Sequence conflict3611V → L in CAA61856. Ref.1
Sequence conflict3721L → H in CAA61856. Ref.1
Sequence conflict381 – 3833RTR → AP in CAA61856. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48638 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 29C1F06CE2195888

FASTA45949,478
        10         20         30         40         50         60 
MTFDYDLFVI GAGSGGLAAS KRAASYGAKV AIAENDLVGG TCVIRGCVPK KLMVYGSHFP 

        70         80         90        100        110        120 
ALFEDAAGYG WQVGKAELNW EHFITSIDKE VRRLSQLHIS FLEKAGVELI SGRATLVDNH 

       130        140        150        160        170        180 
TVEVGERKFT ADKILIAVGG RPIKPELPGM EYGITSNEIF HLKTQPKHIA IIGSGYIGTE 

       190        200        210        220        230        240 
FAGIMRGLGS QVTQITRGDK ILKGFDEDIR TEIQEGMTNH GIRIIPKNVV TAIEQVPEGL 

       250        260        270        280        290        300 
KISLSGEDQE PIIADVFLVA TGRVPNVDGL GLENAGVDVV DSSIEGPGYS TMNAIAVNEY 

       310        320        330        340        350        360 
SQTSQPNIYA VGDVTDRLNL TPVAIGEGRA FADSEFGNNR REFSHETIAT AVFSNPQAST 

       370        380        390        400        410        420 
VGLTEAEARA KLGDDAVTIY RTRFRPMYHS FTGKQERIMM KLVVDTKTDK VLGAHMVGEN 

       430        440        450 
AAEIIQGVAI AVKMGATKKD FDATVGIHPS SAEEFVTMR 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing, and regulation of the glutathione reductase gene from the cyanobacterium Anabaena PCC 7120."
Jiang F., Hellman U., Sroga G.E., Bergman B., Mannervik B.
J. Biol. Chem. 270:22882-22889(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89712 Genomic DNA. Translation: CAA61856.1.
BA000019 Genomic DNA. Translation: BAB76667.1.
PIRAH2426.
I39477.
RefSeqNP_489008.1. NC_003272.1.

3D structure databases

ProteinModelPortalP48638.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.all4968.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB76667; BAB76667; BAB76667.
GeneID1108569.
KEGGana:all4968.
PATRIC22780609. VBINosSp37423_5746.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1249.
HOGENOMHOG000276712.
KOK00383.
OMAANVEANC.
OrthoDBEOG6QCD6D.

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01424. gluta_reduc_2. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHR_NOSS1
AccessionPrimary (citable) accession number: P48638
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2002
Last modified: May 14, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families