P48629 (FAD6C_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Omega-6 fatty acid desaturase, chloroplastic
EC=1.14.19.-

Gene names

Name:

FAD6

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chloroplast omega-6 fatty acid desaturase introduces the second double bond in the biosynthesis of 16:3 and 18:3 fatty acids, important constituents of plant membranes. It is thought to use ferredoxin as an electron donor and to act on fatty acids esterified to galactolipids, sulfolipids and phosphatidylglycerol.
Pathway	Lipid metabolism; polyunsaturated fatty acid biosynthesis.
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane protein Probable.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the fatty acid desaturase family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	unsaturated fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 65	65	Chloroplast Ref.1
	Chain	66 – 447	382	Omega-6 fatty acid desaturase, chloroplastic		PRO_0000007126
Regions
	Motif	171 – 175	5	Histidine box-1
	Motif	207 – 211	5	Histidine box-2
	Motif	367 – 371	5	Histidine box-3

Sequences

	Sequence		Length	Mass (Da)
	P48629 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 2BA7C87FFF95350E	FASTA	447	51,306
	10 20 30 40 50 60 MESAITISNH VNLAFSLSRN PSLSTKNSAG ISCIKWQRPC LRNLGHVRLN QQRKGTRRKS 70 80 90 100 110 120 TLVQAVAVPV AQPSAFPPTD NTEHLKQLAE RYGFQQIGEP LPDDVTMRDI ITSLPKQVFE 130 140 150 160 170 180 INDTKAWGTV LISVTSYALG IFMIAKAPWY LLPLAWAWTG TAITGFFVIG HDCAHKSFSK 190 200 210 220 230 240 NKLVEDIVGT LAFMPLIYPY EPWRFKHDQH HTKTNMLRED TAWLPIMKED IESSPGLRKA 250 260 270 280 290 300 LIYAYGPLRT WMSIAHWLKV HFNLKDFRQS EVKRATISLA AVFAFMVIGW PLIIYKTGIV 310 320 330 340 350 360 GWIKFWLMPW LGYHFWMSTF TIVHHTAPHI PFKSSKEWNA AQAQLSGTVH CDYPRWIEIL 370 380 390 400 410 420 CHDISVHIPH HISPKIPSYN LRAANQSLNE NWGEYLNKPK SNWRLMRTIM TTCHIYDKDG 430 440 NYVSFEKAVP EESQPISIPK RVMPDYA

References

[1]	"Purification and PCR-based cDNA cloning of a plastidial n-6 desaturase." Schmidt H., Dresselhaus T., Buck F., Heinz E. Plant Mol. Biol. 26:631-642(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 66-78. Strain: cv. Subito. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78311 mRNA. Translation: CAA55121.1.
PIR	S53309.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14124.
UniPathway	UPA00658.
Family and domain databases
InterPro	IPR005804. Fatty_acid_desaturase-1. [Graphical view]
Pfam	PF00487. FA_desaturase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAD6C_SPIOL

Accession

Primary (citable) accession number: P48629

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program