ID EPO_BOVIN Reviewed; 192 AA. AC P48617; A3FFT0; DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1996, sequence version 1. DT 24-JAN-2024, entry version 130. DE RecName: Full=Erythropoietin; DE Flags: Precursor; GN Name=EPO; OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; OC Bovinae; Bos. OX NCBI_TaxID=9913; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=Boran; TISSUE=Kidney; RX PubMed=8666286; DOI=10.1016/0378-1119(95)00895-0; RA Suliman H.B., Majiwa P.A.O., Feldman B.F., Mertens B., Logan-Henfrey L.L.; RT "Cloning of a cDNA encoding bovine erythropoietin and analysis of its RT transcription in selected tissues."; RL Gene 171:275-280(1996). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Jin S., Zhong J.C., Chen Z.H., Ma Z.J.; RT "Cloning and sequence analysis on erythropoietin (EPO) gene of Bos taurus RT (San Jiang yellow cattle)."; RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Hormone involved in the regulation of erythrocyte CC proliferation and differentiation and the maintenance of a CC physiological level of circulating erythrocyte mass. Binds to EPOR CC leading to EPOR dimerization and JAK2 activation thereby activating CC specific downstream effectors, including STAT1 and STAT3. CC {ECO:0000250|UniProtKB:P01588}. CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- TISSUE SPECIFICITY: Produced by kidney or liver of adult mammals and by CC liver of fetal or neonatal mammals. CC -!- SIMILARITY: Belongs to the EPO/TPO family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L41354; AAB41268.1; -; mRNA. DR EMBL; U44762; AAA86653.1; -; mRNA. DR EMBL; EF374049; ABN48309.1; -; Genomic_DNA. DR RefSeq; NP_776334.1; NM_173909.2. DR AlphaFoldDB; P48617; -. DR SMR; P48617; -. DR STRING; 9913.ENSBTAP00000072843; -. DR GlyCosmos; P48617; 3 sites, No reported glycans. DR PaxDb; 9913-ENSBTAP00000004450; -. DR Ensembl; ENSBTAT00000072093.1; ENSBTAP00000072843.1; ENSBTAG00000003430.4. DR GeneID; 280784; -. DR KEGG; bta:280784; -. DR CTD; 2056; -. DR VEuPathDB; HostDB:ENSBTAG00000003430; -. DR VGNC; VGNC:28549; EPO. DR eggNOG; ENOG502RXRC; Eukaryota. DR GeneTree; ENSGT00390000017226; -. DR HOGENOM; CLU_110946_0_0_1; -. DR InParanoid; P48617; -. DR OMA; AMEFPRL; -. DR OrthoDB; 4338688at2759; -. DR TreeFam; TF333413; -. DR Reactome; R-BTA-9027276; Erythropoietin activates Phosphoinositide-3-kinase (PI3K). DR Reactome; R-BTA-9027284; Erythropoietin activates RAS. DR Proteomes; UP000009136; Chromosome 25. DR Bgee; ENSBTAG00000003430; Expressed in cumulus cell and 11 other cell types or tissues. DR GO; GO:0009986; C:cell surface; IEA:Ensembl. DR GO; GO:0005615; C:extracellular space; IBA:GO_Central. DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central. DR GO; GO:0005128; F:erythropoietin receptor binding; ISS:UniProtKB. DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW. DR GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central. DR GO; GO:0071474; P:cellular hyperosmotic response; IEA:Ensembl. DR GO; GO:0007566; P:embryo implantation; IEA:Ensembl. DR GO; GO:0030218; P:erythrocyte differentiation; ISS:UniProtKB. DR GO; GO:0043249; P:erythrocyte maturation; IEA:UniProtKB-KW. DR GO; GO:0038162; P:erythropoietin-mediated signaling pathway; ISS:UniProtKB. DR GO; GO:0042541; P:hemoglobin biosynthetic process; IEA:Ensembl. DR GO; GO:0033028; P:myeloid cell apoptotic process; IEA:Ensembl. DR GO; GO:0010523; P:negative regulation of calcium ion transport into cytosol; IEA:Ensembl. DR GO; GO:1902251; P:negative regulation of erythrocyte apoptotic process; IEA:Ensembl. DR GO; GO:1902219; P:negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress; IEA:Ensembl. DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl. DR GO; GO:0008284; P:positive regulation of cell population proliferation; IBA:GO_Central. DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IEA:Ensembl. DR GO; GO:0046579; P:positive regulation of Ras protein signal transduction; IBA:GO_Central. DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; IEA:Ensembl. DR GO; GO:0001666; P:response to hypoxia; IEA:Ensembl. DR Gene3D; 1.20.1250.10; -; 1. DR InterPro; IPR009079; 4_helix_cytokine-like_core. DR InterPro; IPR019767; EPO/TPO_CS. DR InterPro; IPR001323; EPO_TPO. DR InterPro; IPR003013; Erythroptn. DR PANTHER; PTHR10370; ERYTHROPOIETIN; 1. DR PANTHER; PTHR10370:SF0; ERYTHROPOIETIN; 1. DR Pfam; PF00758; EPO_TPO; 1. DR PIRSF; PIRSF001951; EPO; 1. DR PRINTS; PR00272; ERYTHROPTN. DR SUPFAM; SSF47266; 4-helical cytokines; 1. DR PROSITE; PS00817; EPO_TPO; 1. PE 2: Evidence at transcript level; KW Disulfide bond; Erythrocyte maturation; Glycoprotein; Hormone; KW Reference proteome; Secreted; Signal. FT SIGNAL 1..25 FT /evidence="ECO:0000255" FT CHAIN 26..192 FT /note="Erythropoietin" FT /id="PRO_0000008397" FT CARBOHYD 49 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 63 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 108 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT DISULFID 32..187 FT /evidence="ECO:0000250" FT DISULFID 54..58 FT /evidence="ECO:0000250" SQ SEQUENCE 192 AA; 21076 MW; DBC419022F7B483A CRC64; MGARDCTPLL MLSFLLFPLG FPVLGAPARL ICDSRVLERY ILEAREAENA TMGCAEGCSF NENITVPDTK VNFYAWKRME VQQQALEVWQ GLALLSEAIL RGQALLANAS QPCEALRLHV DKAVSGLRSL TSLLRALGAQ KEAISLPDAT PSAAPLRAFT VDALSKLFRI YSNFLRGKLT LYTGEACRRG DR //