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Protein

Protein Wnt-10b

Gene

Wnt10b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.1 Publication

GO - Molecular functioni

  • frizzled binding Source: GO_Central
  • receptor binding Source: MGI

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • bone trabecula formation Source: MGI
  • canonical Wnt signaling pathway Source: MGI
  • cell-cell signaling Source: MGI
  • cell cycle arrest Source: MGI
  • cell fate commitment Source: GO_Central
  • cellular response to cAMP Source: MGI
  • cellular response to hydrostatic pressure Source: Ensembl
  • cellular response to organic cyclic compound Source: MGI
  • cellular response to organic substance Source: MGI
  • cellular response to parathyroid hormone stimulus Source: MGI
  • chondrocyte differentiation Source: Ensembl
  • fungiform papilla development Source: MGI
  • G2/M transition of mitotic cell cycle Source: MGI
  • hematopoietic stem cell proliferation Source: MGI
  • lipid metabolic process Source: MGI
  • myoblast differentiation involved in skeletal muscle regeneration Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • negative regulation of fat cell differentiation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neuron differentiation Source: GO_Central
  • positive regulation of anagen Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of bone mineralization Source: MGI
  • positive regulation of canonical Wnt signaling pathway Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of epithelial cell differentiation Source: MGI
  • positive regulation of G2/M transition of mitotic cell cycle Source: MGI
  • positive regulation of ossification Source: MGI
  • positive regulation of osteoblast differentiation Source: MGI
  • positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: MGI
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein stabilization Source: MGI
  • regulation of fat cell differentiation Source: MGI
  • regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
  • regulation of protein metabolic process Source: MGI
  • regulation of skeletal muscle tissue development Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • sensory perception of taste Source: MGI
  • signal transduction Source: MGI
  • skeletal muscle fiber development Source: MGI
  • skeletal muscle tissue regeneration Source: MGI
  • smoothened signaling pathway Source: MGI
  • Wnt signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-10b
Alternative name(s):
Protein Wnt-12
Gene namesi
Name:Wnt10b
Synonyms:Wnt-10b, Wnt10, Wnt12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:108061. Wnt10b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000004146429 – 389Protein Wnt-10bAdd BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei46PhosphothreonineBy similarity1
Disulfide bondi83 ↔ 94By similarity
Glycosylationi93N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi136 ↔ 144By similarity
Disulfide bondi146 ↔ 199By similarity
Disulfide bondi247 ↔ 261By similarity
Disulfide bondi249 ↔ 256By similarity
Lipidationi253O-palmitoleyl serine; by PORCNBy similarity1
Disulfide bondi334 ↔ 349By similarity
Glycosylationi335N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi364 ↔ 379By similarity
Disulfide bondi366 ↔ 376By similarity
Disulfide bondi371 ↔ 372By similarity

Post-translational modificationi

Palmitoleylation is required for efficient binding to frizzled receptors. Depalmitoleylation leads to Wnt signaling pathway inhibition.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP48614.
PRIDEiP48614.

PTM databases

iPTMnetiP48614.
PhosphoSitePlusiP48614.

Expressioni

Tissue specificityi

Expressed in embryos and in the mammary gland of non-pregnant mice.

Gene expression databases

BgeeiENSMUSG00000022996.
CleanExiMM_WNT10B.
GenevisibleiP48614. MM.

Interactioni

GO - Molecular functioni

  • frizzled binding Source: GO_Central
  • receptor binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023732.

Structurei

3D structure databases

ProteinModelPortaliP48614.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
GeneTreeiENSGT00690000101857.
HOGENOMiHOG000039528.
HOVERGENiHBG001595.
InParanoidiP48614.
KOiK01357.
OMAiRNPHASN.
OrthoDBiEOG091G0OFF.
PhylomeDBiP48614.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013302. Wnt10.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 2 hits.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01893. WNT10PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P48614-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLEEPRSRPP PLGLAGLLFL ALFSRALSNE ILGLKLPGEP PLTANTVCLT
60 70 80 90 100
LSGLSKRQLG LCLRSPDVTA SALQGLHIAV HECQHQLRDQ RWNCSALEGG
110 120 130 140 150
GRLPHHSAIL KRGFRESAFS FSMLAAGVMH AVATACSLGK LVSCGCGWKG
160 170 180 190 200
SGEQDRLRAK LLQLQALSRG KTFPISQPSP VPGSVPSPGP QDTWEWGGCN
210 220 230 240 250
HDMDFGEKFS RDFLDSREAP RDIQARMRIH NNRVGRQVVT ENLKRKCKCH
260 270 280 290 300
GTSGSCQFKT CWRAAPEFRA IGAALRERLS RAIFIDTHNR NSGAFQPRLR
310 320 330 340 350
PRRLSGELVY FEKSPDFCER DPTLGSPGTR GRACNKTSRL LDGCGSLCCG
360 370 380
RGHNVLRQTR VERCHCRFHW CCYVLCDECK VTEWVNVCK
Length:389
Mass (Da):43,119
Last modified:February 1, 1996 - v1
Checksum:iF990AB33B7C84633
GO
Isoform Short (identifier: P48614-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     143-238: Missing.

Show »
Length:293
Mass (Da):32,400
Checksum:iE2BB87BC40C40240
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006796143 – 238Missing in isoform Short. 1 PublicationAdd BLAST96

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20658 mRNA. Translation: AAA80110.1.
U30464 mRNA. Translation: AAA84399.1.
U61970 mRNA. Translation: AAB08086.1.
U61971 mRNA. Translation: AAB08087.1.
CCDSiCCDS27806.1. [P48614-1]
PIRiA59393. A59392.
I49263.
RefSeqiNP_035848.1. NM_011718.2. [P48614-1]
XP_006520954.1. XM_006520891.3. [P48614-1]
XP_006520955.1. XM_006520892.3. [P48614-2]
UniGeneiMm.4709.

Genome annotation databases

EnsembliENSMUST00000023732; ENSMUSP00000023732; ENSMUSG00000022996. [P48614-1]
ENSMUST00000166022; ENSMUSP00000131056; ENSMUSG00000022996. [P48614-1]
GeneIDi22410.
KEGGimmu:22410.
UCSCiuc007xns.2. mouse. [P48614-2]
uc007xnt.2. mouse. [P48614-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20658 mRNA. Translation: AAA80110.1.
U30464 mRNA. Translation: AAA84399.1.
U61970 mRNA. Translation: AAB08086.1.
U61971 mRNA. Translation: AAB08087.1.
CCDSiCCDS27806.1. [P48614-1]
PIRiA59393. A59392.
I49263.
RefSeqiNP_035848.1. NM_011718.2. [P48614-1]
XP_006520954.1. XM_006520891.3. [P48614-1]
XP_006520955.1. XM_006520892.3. [P48614-2]
UniGeneiMm.4709.

3D structure databases

ProteinModelPortaliP48614.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023732.

PTM databases

iPTMnetiP48614.
PhosphoSitePlusiP48614.

Proteomic databases

PaxDbiP48614.
PRIDEiP48614.

Protocols and materials databases

DNASUi22410.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023732; ENSMUSP00000023732; ENSMUSG00000022996. [P48614-1]
ENSMUST00000166022; ENSMUSP00000131056; ENSMUSG00000022996. [P48614-1]
GeneIDi22410.
KEGGimmu:22410.
UCSCiuc007xns.2. mouse. [P48614-2]
uc007xnt.2. mouse. [P48614-1]

Organism-specific databases

CTDi7480.
MGIiMGI:108061. Wnt10b.

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
GeneTreeiENSGT00690000101857.
HOGENOMiHOG000039528.
HOVERGENiHBG001595.
InParanoidiP48614.
KOiK01357.
OMAiRNPHASN.
OrthoDBiEOG091G0OFF.
PhylomeDBiP48614.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

PROiP48614.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022996.
CleanExiMM_WNT10B.
GenevisibleiP48614. MM.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013302. Wnt10.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 2 hits.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01893. WNT10PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWN10B_MOUSE
AccessioniPrimary (citable) accession number: P48614
Secondary accession number(s): P70702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.