P48614 (WN10B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-10b

Alternative name(s):
Protein Wnt-12

Gene names

Name:	Wnt10b
Synonyms:	Wnt-10b, Wnt10, Wnt12

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Expressed in embryos and in the mammary gland of non-pregnant mice.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G2/M transition of mitotic cell cycle Inferred from genetic interaction. Source: MGI axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome bone trabecula formation Inferred from mutant phenotype. Source: MGI canonical Wnt receptor signaling pathway Inferred from direct assay. Source: MGI cell cycle arrest Inferred from direct assay. Source: MGI cell-cell signaling Traceable author statement. Source: MGI cellular response to cAMP Inferred from direct assay. Source: MGI cellular response to organic cyclic compound Inferred from direct assay. Source: MGI cellular response to parathyroid hormone stimulus Inferred from direct assay. Source: MGI female gonad development Inferred from Biological aspect of Ancestor. Source: RefGenome fungiform papilla development Inferred from mutant phenotype. Source: MGI hemopoietic stem cell proliferation Inferred from direct assay. Source: MGI lipid metabolic process Inferred from mutant phenotype. Source: MGI midbrain-hindbrain boundary development Inferred from Biological aspect of Ancestor. Source: RefGenome myoblast cell differentiation involved in skeletal muscle regeneration Inferred from mutant phenotype. Source: MGI negative regulation of epithelial cell proliferation Inferred from direct assay. Source: MGI negative regulation of fat cell differentiation Inferred from direct assay. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI positive regulation of G2/M transition of mitotic cell cycle Inferred from direct assay. Source: MGI positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Inferred from direct assay. Source: MGI positive regulation of anagen Inferred from direct assay. Source: MGI positive regulation of bone mineralization Inferred from direct assay. Source: MGI positive regulation of canonical Wnt receptor signaling pathway Inferred from direct assay. Source: MGI positive regulation of cell proliferation Inferred from direct assay. Source: MGI positive regulation of epithelial cell differentiation Inferred from direct assay. Source: MGI positive regulation of osteoblast differentiation Inferred from direct assay. Source: MGI positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: MGI regulation of proteasomal ubiquitin-dependent protein catabolic process Inferred from direct assay. Source: MGI regulation of skeletal muscle tissue development Inferred from mutant phenotype. Source: MGI sensory perception of taste Inferred from mutant phenotype. Source: MGI skeletal muscle fiber development Inferred from mutant phenotype. Source: MGI smoothened signaling pathway Inferred from direct assay. Source: MGI
Cellular component	extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P48614-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P48614-2)

The sequence of this isoform differs from the canonical sequence as follows:
     143-238: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 389	361	Protein Wnt-10b		PRO_0000041464
Amino acid modifications
	Modified residue	46	1	Phosphothreonine By similarity
	Modified residue	50	1	Phosphothreonine By similarity
	Modified residue	52	1	Phosphoserine By similarity
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	143 – 238	96	Missing in isoform Short.		VSP_006796

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F990AB33B7C84633	FASTA	389	43,119
	10 20 30 40 50 60 MLEEPRSRPP PLGLAGLLFL ALFSRALSNE ILGLKLPGEP PLTANTVCLT LSGLSKRQLG 70 80 90 100 110 120 LCLRSPDVTA SALQGLHIAV HECQHQLRDQ RWNCSALEGG GRLPHHSAIL KRGFRESAFS 130 140 150 160 170 180 FSMLAAGVMH AVATACSLGK LVSCGCGWKG SGEQDRLRAK LLQLQALSRG KTFPISQPSP 190 200 210 220 230 240 VPGSVPSPGP QDTWEWGGCN HDMDFGEKFS RDFLDSREAP RDIQARMRIH NNRVGRQVVT 250 260 270 280 290 300 ENLKRKCKCH GTSGSCQFKT CWRAAPEFRA IGAALRERLS RAIFIDTHNR NSGAFQPRLR 310 320 330 340 350 360 PRRLSGELVY FEKSPDFCER DPTLGSPGTR GRACNKTSRL LDGCGSLCCG RGHNVLRQTR 370 380 VERCHCRFHW CCYVLCDECK VTEWVNVCK
	Isoform Short [UniParc]. Checksum: E2BB87BC40C40240	FASTA	293	32,400
	10 20 30 40 50 60 MLEEPRSRPP PLGLAGLLFL ALFSRALSNE ILGLKLPGEP PLTANTVCLT LSGLSKRQLG 70 80 90 100 110 120 LCLRSPDVTA SALQGLHIAV HECQHQLRDQ RWNCSALEGG GRLPHHSAIL KRGFRESAFS 130 140 150 160 170 180 FSMLAAGVMH AVATACSLGK LVVTENLKRK CKCHGTSGSC QFKTCWRAAP EFRAIGAALR 190 200 210 220 230 240 ERLSRAIFID THNRNSGAFQ PRLRPRRLSG ELVYFEKSPD FCERDPTLGS PGTRGRACNK 250 260 270 280 290 TSRLLDGCGS LCCGRGHNVL RQTRVERCHC RFHWCCYVLC DECKVTEWVN VCK

References

[1]	"Insertional mutagenesis identifies a member of the Wnt gene family as a candidate oncogene in the mammary epithelium of int-2/Fgf-3 transgenic mice." Lee F.S., Lane T.F., Kuo A., Shackleford G.M., Leder P. Proc. Natl. Acad. Sci. U.S.A. 92:2268-2272(1995) [PubMed: 7892260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss Webster. Tissue: Embryo.
[2]	"The mouse Wnt-10B gene isolated from helper T cells is widely expressed and a possible oncogene in BR6 mouse mammary tumorigenesis." Hardiman G., Albright S., Tsunoda J., McClanahan T., Lee F. Gene 172:199-205(1996) [PubMed: 8682303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Murine Wnt10a and Wnt10b: cloning and expression in developing limbs, face and skin of embryos and in adults." Wang J., Shackleford G.M. Oncogene 13:1537-1544(1996) [PubMed: 8875992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20658 mRNA. Translation: AAA80110.1. U30464 mRNA. Translation: AAA84399.1. U61970 mRNA. Translation: AAB08086.1. U61971 mRNA. Translation: AAB08087.1.
IPI	IPI00113831. IPI00227061.
PIR	A59392. A59393. I49263.
RefSeq	NP_035848.1. NM_011718.2.
UniGene	Mm.4709.
3D structure databases
ProteinModelPortal	P48614.
ModBase	Search...
Protein-protein interaction databases
STRING	P48614.
PTM databases
PhosphoSite	P48614.
Proteomic databases
PRIDE	P48614.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023732; ENSMUSP00000023732; ENSMUSG00000022996. ENSMUST00000166022; ENSMUSP00000131056; ENSMUSG00000022996.
GeneID	22410.
KEGG	mmu:22410.
UCSC	uc007xns.2. mouse. uc007xnt.2. mouse.
Organism-specific databases
CTD	7480.
MGI	MGI:108061. Wnt10b.
Phylogenomic databases
eggNOG	roNOG10325.
HOGENOM	HBG446188.
HOVERGEN	HBG001595.
InParanoid	P48614.
OMA	GCGWKGS.
OrthoDB	EOG4B8JD8.
PhylomeDB	P48614.
Gene expression databases
ArrayExpress	P48614.
Bgee	P48614.
CleanEx	MM_WNT10B.
Genevestigator	P48614.
GermOnline	ENSMUSG00000022996. Mus musculus.
Family and domain databases
InterPro	IPR005817. Wnt. IPR013302. Wnt10. IPR018161. Wnt_grthfactor_CS. [Graphical view]
KO	K01357.
PANTHER	PTHR12027. Wnt. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01893. WNT10PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302809.
SOURCE	Search...

Entry information

Entry name

WN10B_MOUSE

Accession

Primary (citable) accession number: P48614
Secondary accession number(s): P70702

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program