P48612 (PELO_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein pelota
EC=3.1.-.-

Gene names

Name:	pelo
ORF Names:	CG3959

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required prior to the first meiotic division for spindle formation and nuclear envelope breakdown during spermatogenesis. It is also required for normal eye patterning and for mitotic divisions in the ovary. Required for ovarian germ line stem cell self-renewal. May play a role in regulating translation. May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. May have ribonuclease activity Potential. Ref.1 Ref.5
Cofactor	Divalent metal cations Potential.
Subcellular location	Nucleus Potential. Cytoplasm Ref.5.
Tissue specificity	Expressed throughout all development stages. Highest levels in early embryo (0-2h) and adult stages. Ref.1
Domain	The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity Potential.
Sequence similarities	Belongs to the eukaryotic release factor 1 family. Pelota subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	compound eye morphogenesis Inferred from mutant phenotype Ref.1. Source: FlyBase female germ-line stem cell division Inferred from mutant phenotype Ref.5. Source: FlyBase germ-line stem cell maintenance Inferred from mutant phenotype Ref.5. Source: FlyBase male meiosis I Traceable author statement. Source: FlyBase meiotic G2/MI transition Traceable author statement. Source: FlyBase meiotic nuclear envelope disassembly Inferred from mutant phenotype Ref.1. Source: FlyBase meiotic spindle organization Inferred from mutant phenotype Ref.1. Source: FlyBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW spermatocyte division Inferred from mutant phenotype Ref.1. Source: FlyBase
Cellular component	cytoplasm Inferred from direct assay Ref.5. Source: FlyBase nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Protein pelota		PRO_0000143190
Regions
	Compositional bias	377 – 383	7	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	81	1	L → V in AAC46879. Ref.1
	Sequence conflict	198	1	N → D in AAC46879. Ref.1
	Sequence conflict	389	1	V → A in AAC46879. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48612 [UniParc]. Last modified September 13, 2005. Version 2. Checksum: 66B41D62BEEB43D3	FASTA	395	44,212
	10 20 30 40 50 60 MKLLGKYVDK GMQGNVTLVP EESEDMWHAY NLIAKGDSVR STTIRKVQNE TATGSSTSSR 70 80 90 100 110 120 VRTTLTIAVE SIDFDTQACV LRLKGRNIEE NQYVKMGAYH TLDLELNRKF ELRKPEWDTI 130 140 150 160 170 180 ALERIEMACD PTQSADVAAV VMQEGLAHVC LITASMTLVR SKIEVSIPRK RKGSVQQHEK 190 200 210 220 230 240 GLAKFYEQVM QSILRHVNFD VVKCVLIASP GFVRDQFYDY MFQQAVKMDY KLLLDNKSKF 250 260 270 280 290 300 MLVHASSGFK HSLREILQDP AVLAKMSDTK AAGEVKALEQ FYMMLQCEPA KAFYGKKHVL 310 320 330 340 350 360 QAAESQAIET LLISDNLFRC QDVSLRKEYV NLVESIRDAG GEVKIFSSMH ISGEQLAQLT 370 380 390 GIAALLRFPM PELEDSDDDD DEDGAAGGVA DSDSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The pelota locus encodes a protein required for meiotic cell division: an analysis of G2/M arrest in Drosophila spermatogenesis." Eberhart C.G., Wasserman S.W. Development 121:3477-3486(1995) [PubMed: 7588080] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Pelota controls self-renewal of germline stem cells by repressing a Bam-independent differentiation pathway." Xi R., Doan C., Liu D., Xie T. Development 132:5365-5374(2005) [PubMed: 16280348] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27197 mRNA. Translation: AAC46879.1. AY058644 mRNA. Translation: AAL13873.1. AE014134 Genomic DNA. Translation: AAF52799.1.
PIR	T47122.
RefSeq	NP_476982.1. NM_057634.4.
3D structure databases
ProteinModelPortal	P48612.
SMR	P48612. Positions 9-379.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48612. 3 interactions.
MINT	MINT-936154.
STRING	P48612.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0079847; FBpp0079444; FBgn0011207.
GeneID	34286.
KEGG	dme:Dmel_CG3959.
NMPDR	fig|7227.3.peg.1632.
UCSC	CG3959-RA. d. melanogaster.
Organism-specific databases
CTD	53918.
FlyBase	FBgn0011207. pelo.
Phylogenomic databases
eggNOG	inNOG04816.
GeneTree	EMGT00050000016655.
InParanoid	P48612.
OMA	NFDVVKC.
OrthoDB	EOG4QBZMW.
PhylomeDB	P48612.
Gene expression databases
Bgee	P48612.
GermOnline	CG3959. Drosophila melanogaster.
Family and domain databases
InterPro	IPR005140. eRF1_1_Pelota. IPR005141. eRF1_2. IPR005142. eRF1_3. IPR004405. Transl_rel_pelota-like. [Graphical view]
KO	K06965.
PANTHER	PTHR10853. PelA. 1 hit.
Pfam	PF03463. eRF1_1. 1 hit. PF03464. eRF1_2. 1 hit. PF03465. eRF1_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00111. Pelota. 1 hit.
ProtoNet	Search...
Other
NextBio	787756.

Entry information

Entry name

PELO_DROME

Accession

Primary (citable) accession number: P48612
Secondary accession number(s): Q9VL97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project