Reviewed, UniProtKB/Swiss-Prot P48612 (PELO_DROME)

Names and origin

Protein names

Recommended name:
    Protein pelota
    EC=3.1.-.-

Gene names

Name:	pelo
ORF Names:	CG3959

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required prior to the first meiotic division for spindle formation and nuclear envelope breakdown during spermatogenesis. It is also required for normal eye patterning and for mitotic divisions in the ovary. Required for ovarian germ line stem cell self-renewal. May play a role in regulating translation. May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. May have ribonuclease activity Potential.
Cofactor	Divalent metal cations Potential.
Subcellular location	Nucleus Potential. Cytoplasm.
Tissue specificity	Expressed throughout all development stages. Highest levels in early embryo (0-2h) and adult stages. Ref.1
Domain	The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity Potential.
Sequence similarities	Belongs to the eukaryotic release factor 1 family. Pelota subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	compound eye morphogenesis Ref.1 Inferred from mutant phenotype. Source: FlyBase female germ-line stem cell division Ref.5 Inferred from mutant phenotype. Source: FlyBase germ-line stem cell maintenance Ref.5 Inferred from mutant phenotype. Source: FlyBase male meiosis I Traceable author statement. Source: FlyBase meiotic G2/MI transition Traceable author statement. Source: FlyBase meiotic nuclear envelope disassembly Ref.1 Inferred from mutant phenotype. Source: FlyBase meiotic spindle organization Ref.1 Inferred from mutant phenotype. Source: FlyBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW spermatocyte division Ref.1 Inferred from mutant phenotype. Source: FlyBase translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.5 Inferred from direct assay. Source: FlyBase nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
chico	Q9XTN2	1	EBI-131708,EBI-176370
H2.0	P10035	1	EBI-131708,EBI-149544
HBS1	Q9W074	1	EBI-131708,EBI-152333

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Protein pelota		PRO_0000143190
Regions
	Compositional bias	377 – 383	7	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	81	1	L → V in AAC46879. Ref.1
	Sequence conflict	198	1	N → D in AAC46879. Ref.1
	Sequence conflict	389	1	V → A in AAC46879. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48612-1 [UniParc]. Last modified September 13, 2005. Version 2. Checksum: 66B41D62BEEB43D3	FASTA	395	44,212
	10 20 30 40 50 60 MKLLGKYVDK GMQGNVTLVP EESEDMWHAY NLIAKGDSVR STTIRKVQNE TATGSSTSSR 70 80 90 100 110 120 VRTTLTIAVE SIDFDTQACV LRLKGRNIEE NQYVKMGAYH TLDLELNRKF ELRKPEWDTI 130 140 150 160 170 180 ALERIEMACD PTQSADVAAV VMQEGLAHVC LITASMTLVR SKIEVSIPRK RKGSVQQHEK 190 200 210 220 230 240 GLAKFYEQVM QSILRHVNFD VVKCVLIASP GFVRDQFYDY MFQQAVKMDY KLLLDNKSKF 250 260 270 280 290 300 MLVHASSGFK HSLREILQDP AVLAKMSDTK AAGEVKALEQ FYMMLQCEPA KAFYGKKHVL 310 320 330 340 350 360 QAAESQAIET LLISDNLFRC QDVSLRKEYV NLVESIRDAG GEVKIFSSMH ISGEQLAQLT 370 380 390 GIAALLRFPM PELEDSDDDD DEDGAAGGVA DSDSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The pelota locus encodes a protein required for meiotic cell division: an analysis of G2/M arrest in Drosophila spermatogenesis." Eberhart C.G., Wasserman S.W. Development 121:3477-3486(1995) [PubMed: 7588080] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Pelota controls self-renewal of germline stem cells by repressing a Bam-independent differentiation pathway." Xi R., Doan C., Liu D., Xie T. Development 132:5365-5374(2005) [PubMed: 16280348] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U27197 mRNA. Translation: AAC46879.1. AY058644 mRNA. Translation: AAL13873.1. AE014134 Genomic DNA. Translation: AAF52799.1.
PIR	T47122.
RefSeq	NP_476982.1.
UniGene	Dm.4742
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P48612. 4 interactions.
Genome annotation databases
Ensembl	FBgn0011207. Drosophila melanogaster. [Contig view]
GeneID	34286.
KEGG	dme:Dmel_CG3959.
NMPDR	fig|7227.3.peg.1632.
Organism-specific databases
FlyBase	FBgn0011207. pelo.
Phylogenomic databases
HOGENOM	P48612.
OMA	P48612. GLAHVCL.
Gene expression databases
ArrayExpress	P48612.
GermOnline	CG3959. Drosophila melanogaster.
Family and domain databases
InterPro	IPR005140. eRF1_1. IPR005141. eRF1_2. IPR005142. eRF1_3. IPR004405. PelA. [Graphical view]
PANTHER	PTHR10853. PelA. 1 hit.
Pfam	PF03463. eRF1_1. 1 hit. PF03464. eRF1_2. 1 hit. PF03465. eRF1_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00111. pelota. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	787756.

Entry information

Entry name

PELO_DROME

Accession

Primary (citable) accession number: P48612
Secondary accession number(s): Q9VL97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project