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Protein

Eukaryotic translation initiation factor 4E

Gene

eif4e

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Maternal RNA in oocytes remain in a dormant state as tacc3/maskin outcompetes eif4g to bind eif4e, thereby preventing translation. During oocyte maturation this complex dissolves and eif4g binds eif4e to allow translation of maternal RNAs.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E
Short name:
eIF-4E
Short name:
eIF4E
Alternative name(s):
eIF-4F 25 kDa subunit
mRNA cap-binding protein
Gene namesi
Name:eif4e
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254191 eif4e1b

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001936401 – 213Eukaryotic translation initiation factor 4EAdd BLAST213

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei205Phosphoserine; by PKCBy similarity1

Post-translational modificationi

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP48597

Expressioni

Developmental stagei

Expressed both maternally and zygotically.2 Publications

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eif4a, eif4e and eif4g. eif4e is also known to interact with other partners. tacc3/maskin competes with eif4g for binding to eif4e.

Binary interactionsi

WithEntry#Exp.IntActNotes
tacc3Q9PTG83EBI-65739,EBI-65726

Protein-protein interaction databases

DIPiDIP-29251N
IntActiP48597, 2 interactors
MINTiP48597

Structurei

3D structure databases

ProteinModelPortaliP48597
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 537-methylguanosine-containing mRNA cap bindingBy similarity2
Regioni98 – 997-methylguanosine-containing mRNA cap bindingBy similarity2
Regioni153 – 1587-methylguanosine-containing mRNA cap bindingBy similarity6
Regioni201 – 2037-methylguanosine-containing mRNA cap bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG006130

Family and domain databases

Gene3Di3.30.760.10, 1 hit
InterProiView protein in InterPro
IPR023398 TIF_eIF4e-like
IPR001040 TIF_eIF_4E
IPR019770 TIF_eIF_4E_CS
PANTHERiPTHR11960 PTHR11960, 1 hit
PfamiView protein in Pfam
PF01652 IF4E, 1 hit
SUPFAMiSSF55418 SSF55418, 1 hit
PROSITEiView protein in PROSITE
PS00813 IF4E, 1 hit

Sequencei

Sequence statusi: Complete.

P48597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVEPENTN PQSTEEEKET GQEIVSPDQY IKHPLQNRWA LWFFKNDKSK
60 70 80 90 100
TWQANLRLIS KFDTVEDFWA LYNHIQLSSN LMSGCDYSLF KDGIEPMWED
110 120 130 140 150
EKNKRGGRWL ITLNKQQRRN DLDRFWLETL MCLIGESFDE HSDDVCGAVV
160 170 180 190 200
NVRAKGDKIA IWTTEFENKD AVTHIGRVYK ERLGLPAKVV IGYQSHADTA
210
TKSGSTTKNR FVV
Length:213
Mass (Da):24,635
Last modified:February 1, 1996 - v1
Checksum:iE9412DFF235AE9E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31837 mRNA Translation: BAA06623.1
PIRiI51413
RefSeqiXP_018090600.1, XM_018235111.1
UniGeneiXl.59

Genome annotation databases

GeneIDi399255

Similar proteinsi

Entry informationi

Entry nameiIF4E_XENLA
AccessioniPrimary (citable) accession number: P48597
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 25, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

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