Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTP cyclohydrolase 1

Gene

Pu

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform B is required for eye pigment production, Isoform C may be required for normal embryonic development and segment pattern formation.1 Publication

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.1 Publication

Pathwayi: 7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (Pu)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi214ZincBy similarity1
Metal bindingi217ZincBy similarity1
Metal bindingi285ZincBy similarity1

GO - Molecular functioni

  • GTP binding Source: GO_Central
  • GTP cyclohydrolase I activity Source: FlyBase
  • GTP cyclohydrolase I regulator activity Source: FlyBase
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
  • compound eye pigmentation Source: FlyBase
  • cuticle pigmentation Source: FlyBase
  • embryonic pattern specification Source: FlyBase
  • larval chitin-based cuticle development Source: FlyBase
  • preblastoderm mitotic cell cycle Source: FlyBase
  • pteridine biosynthetic process Source: FlyBase
  • regulation of epithelial cell migration, open tracheal system Source: FlyBase
  • regulation of GTP cyclohydrolase I activity Source: FlyBase
  • tetrahydrobiopterin biosynthetic process Source: UniProtKB
  • tetrahydrofolate biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18450.
BRENDAi3.5.4.16. 1994.
ReactomeiR-DME-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.161 Publication)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Protein punch
Gene namesi
Name:Pu
ORF Names:CG9441
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003162. Pu.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001194821 – 324GTP cyclohydrolase 1Add BLAST324

Proteomic databases

PaxDbiP48596.
PRIDEiP48596.

Expressioni

Tissue specificityi

Isoform B is expressed almost exclusively in adult heads.1 Publication

Developmental stagei

Isoform C is expressed in embryos, larvae and adults.1 Publication

Gene expression databases

BgeeiFBgn0003162.
ExpressionAtlasiP48596. baseline.
GenevisibleiP48596. DM.

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers.By similarity

Protein-protein interaction databases

BioGridi63049. 3 interactors.
DIPiDIP-24104N.
MINTiMINT-937025.
STRINGi7227.FBpp0071507.

Structurei

3D structure databases

ProteinModelPortaliP48596.
SMRiP48596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiKOG2698. Eukaryota.
COG0302. LUCA.
GeneTreeiENSGT00390000013481.
InParanoidiP48596.
KOiK01495.
OMAiQPKTPMT.
OrthoDBiEOG091G0PCE.
PhylomeDBiP48596.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P48596-1) [UniParc]FASTAAdd to basket
Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFTRQLSEM SASELNDAID DTNFPQAHIL SRGRNNSVCS TSSTSGTSSL
60 70 80 90 100
ADRQQNQAEE ATAIAGTPVE EVAPAPALVP LAGNQRPRLI LKTNGSSPDS
110 120 130 140 150
DGTQPKTPLT PRTSTTPGHE KCTFHHDLEL DHKPPTREAL LPDMARSYRL
160 170 180 190 200
LLGGLGENPD RQGLIKTPER AAKAMLYFTK GYDQSLEDVL NGAVFDEDHD
210 220 230 240 250
EMVVVKDIEM FSMCEHHLVP FYGKVSIGYL PCNKILGLSK LARIVEIFSR
260 270 280 290 300
RLQVQERLTK QIAVAVTQAV QPAGVAVVVE GVHMCMVMRG VQKINSKTVT
310 320
STMLGVFRDD PKTREEFLNL VNSK
Length:324
Mass (Da):35,541
Last modified:January 27, 2003 - v3
Checksum:i712A0045F2C12D6A
GO
Isoform B (identifier: P48596-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-117: MSFTRQLSEM...PLTPRTSTTP → MKPQTSEQNG...LNLELASNGS

Show »
Length:273
Mass (Da):29,812
Checksum:i99B3DA99E8D4B2C2
GO
Isoform C (identifier: P48596-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-117: Missing.

Show »
Length:308
Mass (Da):33,877
Checksum:iA142F66EDE6D3969
GO

Sequence cautioni

The sequence AAC04308 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.Curated
The sequence AAC04309 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.Curated
The sequence AAD44334 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.Curated
The sequence AAR20857 differs from that shown. Reason: Frameshift at position 53.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti58A → T in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11, Raleigh Inbred 27 and Raleigh Inbred 33. 1 Publication1
Natural varianti61A → P in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11, Raleigh Inbred 27 and Raleigh Inbred 33. 1 Publication1
Natural varianti77A → V in strain: Raleigh Inbred 11. 1 Publication1
Natural varianti84N → S in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11 and Raleigh Inbred 33. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0016161 – 117MSFTR…TSTTP → MKPQTSEQNGSGQNGEGAAD AVAVATIPTGEASAASATSG TDLTVSKNSQQLKLEMLNLE LASNGS in isoform B. 1 PublicationAdd BLAST117
Alternative sequenceiVSP_001615102 – 117Missing in isoform C. 1 PublicationAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01118 mRNA. Translation: AAC04308.1. Frameshift.
U01119 mRNA. Translation: AAC04309.1. Frameshift.
AF159422 mRNA. Translation: AAD44334.1. Frameshift.
AY382619 Genomic DNA. Translation: AAR20848.1.
AY382620 Genomic DNA. Translation: AAR20850.1.
AY382622 Genomic DNA. Translation: AAR20852.1.
AY382623 Genomic DNA. Translation: AAR20854.1.
AY382624 Genomic DNA. Translation: AAR20855.1.
AY382625 Genomic DNA. Translation: AAR20857.2. Frameshift.
AY382626 Genomic DNA. Translation: AAR20859.1.
AY382628 Genomic DNA. Translation: AAR20862.1.
AE013599 Genomic DNA. Translation: AAF46690.1.
AE013599 Genomic DNA. Translation: AAF46692.1.
AE013599 Genomic DNA. Translation: AAM70858.1.
AY051890 mRNA. Translation: AAK93314.1.
PIRiA49302.
B49302.
RefSeqiNP_523801.2. NM_079077.4. [P48596-3]
NP_726037.1. NM_166430.3. [P48596-2]
NP_726038.1. NM_166431.4. [P48596-1]
UniGeneiDm.7417.

Genome annotation databases

EnsemblMetazoaiFBtr0071580; FBpp0071507; FBgn0003162. [P48596-1]
GeneIDi37415.
KEGGidme:Dmel_CG9441.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01118 mRNA. Translation: AAC04308.1. Frameshift.
U01119 mRNA. Translation: AAC04309.1. Frameshift.
AF159422 mRNA. Translation: AAD44334.1. Frameshift.
AY382619 Genomic DNA. Translation: AAR20848.1.
AY382620 Genomic DNA. Translation: AAR20850.1.
AY382622 Genomic DNA. Translation: AAR20852.1.
AY382623 Genomic DNA. Translation: AAR20854.1.
AY382624 Genomic DNA. Translation: AAR20855.1.
AY382625 Genomic DNA. Translation: AAR20857.2. Frameshift.
AY382626 Genomic DNA. Translation: AAR20859.1.
AY382628 Genomic DNA. Translation: AAR20862.1.
AE013599 Genomic DNA. Translation: AAF46690.1.
AE013599 Genomic DNA. Translation: AAF46692.1.
AE013599 Genomic DNA. Translation: AAM70858.1.
AY051890 mRNA. Translation: AAK93314.1.
PIRiA49302.
B49302.
RefSeqiNP_523801.2. NM_079077.4. [P48596-3]
NP_726037.1. NM_166430.3. [P48596-2]
NP_726038.1. NM_166431.4. [P48596-1]
UniGeneiDm.7417.

3D structure databases

ProteinModelPortaliP48596.
SMRiP48596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63049. 3 interactors.
DIPiDIP-24104N.
MINTiMINT-937025.
STRINGi7227.FBpp0071507.

Proteomic databases

PaxDbiP48596.
PRIDEiP48596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0071580; FBpp0071507; FBgn0003162. [P48596-1]
GeneIDi37415.
KEGGidme:Dmel_CG9441.

Organism-specific databases

CTDi37415.
FlyBaseiFBgn0003162. Pu.

Phylogenomic databases

eggNOGiKOG2698. Eukaryota.
COG0302. LUCA.
GeneTreeiENSGT00390000013481.
InParanoidiP48596.
KOiK01495.
OMAiQPKTPMT.
OrthoDBiEOG091G0PCE.
PhylomeDBiP48596.

Enzyme and pathway databases

UniPathwayiUPA00848; UER00151.
BioCyciMetaCyc:MONOMER-18450.
BRENDAi3.5.4.16. 1994.
ReactomeiR-DME-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

ChiTaRSiPu. fly.
GenomeRNAii37415.
PROiP48596.

Gene expression databases

BgeeiFBgn0003162.
ExpressionAtlasiP48596. baseline.
GenevisibleiP48596. DM.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCH1_DROME
AccessioniPrimary (citable) accession number: P48596
Secondary accession number(s): Q6TY66
, Q6TY71, Q6TY73, Q6TY74, Q6TY78, Q960S4, Q9W2J9, Q9W2K1, Q9Y0C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 27, 2003
Last modified: November 30, 2016
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.