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P48596

- GCH1_DROME

UniProt

P48596 - GCH1_DROME

Protein

GTP cyclohydrolase 1

Gene

Pu

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 3 (27 Jan 2003)
      Previous versions | rss
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    Functioni

    Isoform B is required for eye pigment production, Isoform C may be required for normal embryonic development and segment pattern formation.1 Publication

    Catalytic activityi

    GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi214 – 2141ZincBy similarity
    Metal bindingi217 – 2171ZincBy similarity
    Metal bindingi285 – 2851ZincBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. GTP cyclohydrolase I activity Source: FlyBase
    3. GTP cyclohydrolase I regulator activity Source: FlyBase
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
    2. compound eye pigmentation Source: FlyBase
    3. cuticle pigmentation Source: FlyBase
    4. embryonic pattern specification Source: FlyBase
    5. larval chitin-based cuticle development Source: FlyBase
    6. preblastoderm mitotic cell cycle Source: FlyBase
    7. pteridine biosynthetic process Source: FlyBase
    8. regulation of epithelial cell migration, open tracheal system Source: FlyBase
    9. regulation of GTP cyclohydrolase I activity Source: FlyBase
    10. tetrahydrobiopterin biosynthetic process Source: UniProtKB-KW
    11. tetrahydrofolate biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Tetrahydrobiopterin biosynthesis

    Keywords - Ligandi

    GTP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18450.
    ReactomeiREACT_180850. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    UniPathwayiUPA00848; UER00151.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP cyclohydrolase 1 (EC:3.5.4.16)
    Alternative name(s):
    GTP cyclohydrolase I
    Short name:
    GTP-CH-I
    Protein punch
    Gene namesi
    Name:Pu
    ORF Names:CG9441
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0003162. Pu.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324GTP cyclohydrolase 1PRO_0000119482Add
    BLAST

    Proteomic databases

    PaxDbiP48596.

    Expressioni

    Tissue specificityi

    Isoform B is expressed almost exclusively in adult heads.1 Publication

    Developmental stagei

    Isoform C is expressed in embryos, larvae and adults.1 Publication

    Gene expression databases

    BgeeiP48596.

    Interactioni

    Subunit structurei

    Toroid-shaped homodecamer, composed of two pentamers of five dimers.By similarity

    Protein-protein interaction databases

    BioGridi63049. 3 interactions.
    DIPiDIP-24104N.
    MINTiMINT-937025.

    Structurei

    3D structure databases

    ProteinModelPortaliP48596.
    SMRiP48596. Positions 138-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GTP cyclohydrolase I family.Curated

    Phylogenomic databases

    eggNOGiCOG0302.
    GeneTreeiENSGT00390000013481.
    InParanoidiP48596.
    KOiK01495.
    OMAiNTRTEIE.
    OrthoDBiEOG77DJ6T.
    PhylomeDBiP48596.

    Family and domain databases

    HAMAPiMF_00223. FolE.
    InterProiIPR001474. GTP_CycHdrlase_I.
    IPR018234. GTP_CycHdrlase_I_CS.
    IPR020602. GTP_CycHdrlase_I_dom.
    [Graphical view]
    PANTHERiPTHR11109. PTHR11109. 1 hit.
    PfamiPF01227. GTP_cyclohydroI. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00063. folE. 1 hit.
    PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
    PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: P48596-1) [UniParc]FASTAAdd to Basket

    Also known as: C

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSFTRQLSEM SASELNDAID DTNFPQAHIL SRGRNNSVCS TSSTSGTSSL    50
    ADRQQNQAEE ATAIAGTPVE EVAPAPALVP LAGNQRPRLI LKTNGSSPDS 100
    DGTQPKTPLT PRTSTTPGHE KCTFHHDLEL DHKPPTREAL LPDMARSYRL 150
    LLGGLGENPD RQGLIKTPER AAKAMLYFTK GYDQSLEDVL NGAVFDEDHD 200
    EMVVVKDIEM FSMCEHHLVP FYGKVSIGYL PCNKILGLSK LARIVEIFSR 250
    RLQVQERLTK QIAVAVTQAV QPAGVAVVVE GVHMCMVMRG VQKINSKTVT 300
    STMLGVFRDD PKTREEFLNL VNSK 324
    Length:324
    Mass (Da):35,541
    Last modified:January 27, 2003 - v3
    Checksum:i712A0045F2C12D6A
    GO
    Isoform B (identifier: P48596-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-117: MSFTRQLSEM...PLTPRTSTTP → MKPQTSEQNG...LNLELASNGS

    Show »
    Length:273
    Mass (Da):29,812
    Checksum:i99B3DA99E8D4B2C2
    GO
    Isoform C (identifier: P48596-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         102-117: Missing.

    Show »
    Length:308
    Mass (Da):33,877
    Checksum:iA142F66EDE6D3969
    GO

    Sequence cautioni

    The sequence AAC04308.1 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.
    The sequence AAC04309.1 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.
    The sequence AAD44334.1 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.
    The sequence AAR20857.2 differs from that shown. Reason: Frameshift at position 53.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti58 – 581A → T in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11, Raleigh Inbred 27 and Raleigh Inbred 33. 1 Publication
    Natural varianti61 – 611A → P in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11, Raleigh Inbred 27 and Raleigh Inbred 33. 1 Publication
    Natural varianti77 – 771A → V in strain: Raleigh Inbred 11. 1 Publication
    Natural varianti84 – 841N → S in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11 and Raleigh Inbred 33. 1 Publication

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 117117MSFTR…TSTTP → MKPQTSEQNGSGQNGEGAAD AVAVATIPTGEASAASATSG TDLTVSKNSQQLKLEMLNLE LASNGS in isoform B. 1 PublicationVSP_001616Add
    BLAST
    Alternative sequencei102 – 11716Missing in isoform C. 1 PublicationVSP_001615Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01118 mRNA. Translation: AAC04308.1. Frameshift.
    U01119 mRNA. Translation: AAC04309.1. Frameshift.
    AF159422 mRNA. Translation: AAD44334.1. Frameshift.
    AY382619 Genomic DNA. Translation: AAR20848.1.
    AY382620 Genomic DNA. Translation: AAR20850.1.
    AY382622 Genomic DNA. Translation: AAR20852.1.
    AY382623 Genomic DNA. Translation: AAR20854.1.
    AY382624 Genomic DNA. Translation: AAR20855.1.
    AY382625 Genomic DNA. Translation: AAR20857.2. Frameshift.
    AY382626 Genomic DNA. Translation: AAR20859.1.
    AY382628 Genomic DNA. Translation: AAR20862.1.
    AE013599 Genomic DNA. Translation: AAF46690.1.
    AE013599 Genomic DNA. Translation: AAF46692.1.
    AE013599 Genomic DNA. Translation: AAM70858.1.
    AY051890 mRNA. Translation: AAK93314.1.
    PIRiA49302.
    B49302.
    RefSeqiNP_523801.2. NM_079077.4. [P48596-3]
    NP_726037.1. NM_166430.3. [P48596-2]
    NP_726038.1. NM_166431.4. [P48596-1]
    UniGeneiDm.7417.

    Genome annotation databases

    EnsemblMetazoaiFBtr0071580; FBpp0071507; FBgn0003162. [P48596-1]
    GeneIDi37415.
    KEGGidme:Dmel_CG9441.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01118 mRNA. Translation: AAC04308.1 . Frameshift.
    U01119 mRNA. Translation: AAC04309.1 . Frameshift.
    AF159422 mRNA. Translation: AAD44334.1 . Frameshift.
    AY382619 Genomic DNA. Translation: AAR20848.1 .
    AY382620 Genomic DNA. Translation: AAR20850.1 .
    AY382622 Genomic DNA. Translation: AAR20852.1 .
    AY382623 Genomic DNA. Translation: AAR20854.1 .
    AY382624 Genomic DNA. Translation: AAR20855.1 .
    AY382625 Genomic DNA. Translation: AAR20857.2 . Frameshift.
    AY382626 Genomic DNA. Translation: AAR20859.1 .
    AY382628 Genomic DNA. Translation: AAR20862.1 .
    AE013599 Genomic DNA. Translation: AAF46690.1 .
    AE013599 Genomic DNA. Translation: AAF46692.1 .
    AE013599 Genomic DNA. Translation: AAM70858.1 .
    AY051890 mRNA. Translation: AAK93314.1 .
    PIRi A49302.
    B49302.
    RefSeqi NP_523801.2. NM_079077.4. [P48596-3 ]
    NP_726037.1. NM_166430.3. [P48596-2 ]
    NP_726038.1. NM_166431.4. [P48596-1 ]
    UniGenei Dm.7417.

    3D structure databases

    ProteinModelPortali P48596.
    SMRi P48596. Positions 138-323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 63049. 3 interactions.
    DIPi DIP-24104N.
    MINTi MINT-937025.

    Proteomic databases

    PaxDbi P48596.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0071580 ; FBpp0071507 ; FBgn0003162 . [P48596-1 ]
    GeneIDi 37415.
    KEGGi dme:Dmel_CG9441.

    Organism-specific databases

    CTDi 37415.
    FlyBasei FBgn0003162. Pu.

    Phylogenomic databases

    eggNOGi COG0302.
    GeneTreei ENSGT00390000013481.
    InParanoidi P48596.
    KOi K01495.
    OMAi NTRTEIE.
    OrthoDBi EOG77DJ6T.
    PhylomeDBi P48596.

    Enzyme and pathway databases

    UniPathwayi UPA00848 ; UER00151 .
    BioCyci MetaCyc:MONOMER-18450.
    Reactomei REACT_180850. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

    Miscellaneous databases

    ChiTaRSi Pu. drosophila.
    GenomeRNAii 37415.
    NextBioi 803517.
    PROi P48596.

    Gene expression databases

    Bgeei P48596.

    Family and domain databases

    HAMAPi MF_00223. FolE.
    InterProi IPR001474. GTP_CycHdrlase_I.
    IPR018234. GTP_CycHdrlase_I_CS.
    IPR020602. GTP_CycHdrlase_I_dom.
    [Graphical view ]
    PANTHERi PTHR11109. PTHR11109. 1 hit.
    Pfami PF01227. GTP_cyclohydroI. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00063. folE. 1 hit.
    PROSITEi PS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
    PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple mRNAs from the Punch locus of Drosophila melanogaster encode isoforms of GTP cyclohydrolase I with distinct N-terminal domains."
      McLean J.R., Krishnakumar S., O'Donnell J.M.
      J. Biol. Chem. 268:27191-27197(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Head.
    2. "Differential regulation of Drosophila GTP cyclohydrolase I isoforms and their interaction with tyrosine hydroxylase."
      Xu D., Neckameyer W., O'Donnell J.
      Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
      Tissue: Embryo.
    3. "Punch affects life history traits in Drosophila melanogaster."
      Geiger-Thornsberry G.L., Harbison S.T., Lyman R.F., Mackay T.F.C.
      Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-58; PRO-61; VAL-77 AND SER-84.
      Strain: Raleigh Inbred 1, Raleigh Inbred 11, Raleigh Inbred 24, Raleigh Inbred 27, Raleigh Inbred 30, Raleigh Inbred 33, Raleigh Inbred 6 and Raleigh Inbred 9.
    4. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    5. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiGCH1_DROME
    AccessioniPrimary (citable) accession number: P48596
    Secondary accession number(s): Q6TY66
    , Q6TY71, Q6TY73, Q6TY74, Q6TY78, Q960S4, Q9W2J9, Q9W2K1, Q9Y0C8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 27, 2003
    Last modified: October 1, 2014
    This is version 127 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3