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P48596

- GCH1_DROME

UniProt

P48596 - GCH1_DROME

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Protein

GTP cyclohydrolase 1

Gene
Pu, CG9441
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Isoform B is required for eye pigment production, Isoform C may be required for normal embryonic development and segment pattern formation.1 Publication

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi214 – 2141Zinc By similarity
Metal bindingi217 – 2171Zinc By similarity
Metal bindingi285 – 2851Zinc By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTP cyclohydrolase I activity Source: FlyBase
  3. GTP cyclohydrolase I regulator activity Source: FlyBase
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 7,8-dihydroneopterin 3'-triphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. compound eye pigmentation Source: FlyBase
  3. cuticle pigmentation Source: FlyBase
  4. embryonic pattern specification Source: FlyBase
  5. larval chitin-based cuticle development Source: FlyBase
  6. preblastoderm mitotic cell cycle Source: FlyBase
  7. pteridine biosynthetic process Source: FlyBase
  8. regulation of epithelial cell migration, open tracheal system Source: FlyBase
  9. regulation of GTP cyclohydrolase I activity Source: FlyBase
  10. tetrahydrobiopterin biosynthetic process Source: UniProtKB-KW
  11. tetrahydrofolate biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18450.
ReactomeiREACT_180850. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Protein punch
Gene namesi
Name:Pu
ORF Names:CG9441
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003162. Pu.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324GTP cyclohydrolase 1UniRule annotationPRO_0000119482Add
BLAST

Proteomic databases

PaxDbiP48596.

Expressioni

Tissue specificityi

Isoform B is expressed almost exclusively in adult heads.1 Publication

Developmental stagei

Isoform C is expressed in embryos, larvae and adults.1 Publication

Gene expression databases

BgeeiP48596.

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers By similarity.

Protein-protein interaction databases

BioGridi63049. 3 interactions.
DIPiDIP-24104N.
MINTiMINT-937025.

Structurei

3D structure databases

ProteinModelPortaliP48596.
SMRiP48596. Positions 138-323.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0302.
GeneTreeiENSGT00390000013481.
InParanoidiP48596.
KOiK01495.
OMAiNTRTEIE.
OrthoDBiEOG77DJ6T.
PhylomeDBiP48596.

Family and domain databases

HAMAPiMF_00223. FolE.
InterProiIPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view]
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform A (identifier: P48596-1) [UniParc]FASTAAdd to Basket

Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSFTRQLSEM SASELNDAID DTNFPQAHIL SRGRNNSVCS TSSTSGTSSL    50
ADRQQNQAEE ATAIAGTPVE EVAPAPALVP LAGNQRPRLI LKTNGSSPDS 100
DGTQPKTPLT PRTSTTPGHE KCTFHHDLEL DHKPPTREAL LPDMARSYRL 150
LLGGLGENPD RQGLIKTPER AAKAMLYFTK GYDQSLEDVL NGAVFDEDHD 200
EMVVVKDIEM FSMCEHHLVP FYGKVSIGYL PCNKILGLSK LARIVEIFSR 250
RLQVQERLTK QIAVAVTQAV QPAGVAVVVE GVHMCMVMRG VQKINSKTVT 300
STMLGVFRDD PKTREEFLNL VNSK 324
Length:324
Mass (Da):35,541
Last modified:January 27, 2003 - v3
Checksum:i712A0045F2C12D6A
GO
Isoform B (identifier: P48596-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-117: MSFTRQLSEM...PLTPRTSTTP → MKPQTSEQNG...LNLELASNGS

Show »
Length:273
Mass (Da):29,812
Checksum:i99B3DA99E8D4B2C2
GO
Isoform C (identifier: P48596-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-117: Missing.

Show »
Length:308
Mass (Da):33,877
Checksum:iA142F66EDE6D3969
GO

Sequence cautioni

The sequence AAC04308.1 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.
The sequence AAC04309.1 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.
The sequence AAD44334.1 differs from that shown. Reason: Frameshift at positions 27, 75, 266 and 271.
The sequence AAR20857.2 differs from that shown. Reason: Frameshift at position 53.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581A → T in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11, Raleigh Inbred 27 and Raleigh Inbred 33. 1 Publication
Natural varianti61 – 611A → P in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11, Raleigh Inbred 27 and Raleigh Inbred 33. 1 Publication
Natural varianti77 – 771A → V in strain: Raleigh Inbred 11. 1 Publication
Natural varianti84 – 841N → S in strain: Raleigh Inbred 6, Raleigh Inbred 9, Raleigh Inbred 11 and Raleigh Inbred 33. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 117117MSFTR…TSTTP → MKPQTSEQNGSGQNGEGAAD AVAVATIPTGEASAASATSG TDLTVSKNSQQLKLEMLNLE LASNGS in isoform B. VSP_001616Add
BLAST
Alternative sequencei102 – 11716Missing in isoform C. VSP_001615Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01118 mRNA. Translation: AAC04308.1. Frameshift.
U01119 mRNA. Translation: AAC04309.1. Frameshift.
AF159422 mRNA. Translation: AAD44334.1. Frameshift.
AY382619 Genomic DNA. Translation: AAR20848.1.
AY382620 Genomic DNA. Translation: AAR20850.1.
AY382622 Genomic DNA. Translation: AAR20852.1.
AY382623 Genomic DNA. Translation: AAR20854.1.
AY382624 Genomic DNA. Translation: AAR20855.1.
AY382625 Genomic DNA. Translation: AAR20857.2. Frameshift.
AY382626 Genomic DNA. Translation: AAR20859.1.
AY382628 Genomic DNA. Translation: AAR20862.1.
AE013599 Genomic DNA. Translation: AAF46690.1.
AE013599 Genomic DNA. Translation: AAF46692.1.
AE013599 Genomic DNA. Translation: AAM70858.1.
AY051890 mRNA. Translation: AAK93314.1.
PIRiA49302.
B49302.
RefSeqiNP_523801.2. NM_079077.4. [P48596-3]
NP_726037.1. NM_166430.3. [P48596-2]
NP_726038.1. NM_166431.4. [P48596-1]
UniGeneiDm.7417.

Genome annotation databases

EnsemblMetazoaiFBtr0071580; FBpp0071507; FBgn0003162. [P48596-1]
GeneIDi37415.
KEGGidme:Dmel_CG9441.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U01118 mRNA. Translation: AAC04308.1 . Frameshift.
U01119 mRNA. Translation: AAC04309.1 . Frameshift.
AF159422 mRNA. Translation: AAD44334.1 . Frameshift.
AY382619 Genomic DNA. Translation: AAR20848.1 .
AY382620 Genomic DNA. Translation: AAR20850.1 .
AY382622 Genomic DNA. Translation: AAR20852.1 .
AY382623 Genomic DNA. Translation: AAR20854.1 .
AY382624 Genomic DNA. Translation: AAR20855.1 .
AY382625 Genomic DNA. Translation: AAR20857.2 . Frameshift.
AY382626 Genomic DNA. Translation: AAR20859.1 .
AY382628 Genomic DNA. Translation: AAR20862.1 .
AE013599 Genomic DNA. Translation: AAF46690.1 .
AE013599 Genomic DNA. Translation: AAF46692.1 .
AE013599 Genomic DNA. Translation: AAM70858.1 .
AY051890 mRNA. Translation: AAK93314.1 .
PIRi A49302.
B49302.
RefSeqi NP_523801.2. NM_079077.4. [P48596-3 ]
NP_726037.1. NM_166430.3. [P48596-2 ]
NP_726038.1. NM_166431.4. [P48596-1 ]
UniGenei Dm.7417.

3D structure databases

ProteinModelPortali P48596.
SMRi P48596. Positions 138-323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 63049. 3 interactions.
DIPi DIP-24104N.
MINTi MINT-937025.

Proteomic databases

PaxDbi P48596.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0071580 ; FBpp0071507 ; FBgn0003162 . [P48596-1 ]
GeneIDi 37415.
KEGGi dme:Dmel_CG9441.

Organism-specific databases

CTDi 37415.
FlyBasei FBgn0003162. Pu.

Phylogenomic databases

eggNOGi COG0302.
GeneTreei ENSGT00390000013481.
InParanoidi P48596.
KOi K01495.
OMAi NTRTEIE.
OrthoDBi EOG77DJ6T.
PhylomeDBi P48596.

Enzyme and pathway databases

UniPathwayi UPA00848 ; UER00151 .
BioCyci MetaCyc:MONOMER-18450.
Reactomei REACT_180850. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

ChiTaRSi Pu. drosophila.
GenomeRNAii 37415.
NextBioi 803517.
PROi P48596.

Gene expression databases

Bgeei P48596.

Family and domain databases

HAMAPi MF_00223. FolE.
InterProi IPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
[Graphical view ]
PANTHERi PTHR11109. PTHR11109. 1 hit.
Pfami PF01227. GTP_cyclohydroI. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00063. folE. 1 hit.
PROSITEi PS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple mRNAs from the Punch locus of Drosophila melanogaster encode isoforms of GTP cyclohydrolase I with distinct N-terminal domains."
    McLean J.R., Krishnakumar S., O'Donnell J.M.
    J. Biol. Chem. 268:27191-27197(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Head.
  2. "Differential regulation of Drosophila GTP cyclohydrolase I isoforms and their interaction with tyrosine hydroxylase."
    Xu D., Neckameyer W., O'Donnell J.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Tissue: Embryo.
  3. "Punch affects life history traits in Drosophila melanogaster."
    Geiger-Thornsberry G.L., Harbison S.T., Lyman R.F., Mackay T.F.C.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-58; PRO-61; VAL-77 AND SER-84.
    Strain: Raleigh Inbred 1, Raleigh Inbred 11, Raleigh Inbred 24, Raleigh Inbred 27, Raleigh Inbred 30, Raleigh Inbred 33, Raleigh Inbred 6 and Raleigh Inbred 9.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiGCH1_DROME
AccessioniPrimary (citable) accession number: P48596
Secondary accession number(s): Q6TY66
, Q6TY71, Q6TY73, Q6TY74, Q6TY78, Q960S4, Q9W2J9, Q9W2K1, Q9Y0C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 27, 2003
Last modified: September 3, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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