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P48594

- SPB4_HUMAN

UniProt

P48594 - SPB4_HUMAN

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Protein

Serpin B4

Gene

SERPINB4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May act as a protease inhibitor to modulate the host immune response against tumor cells.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei354 – 3552Reactive bond

GO - Molecular functioni

  1. enzyme binding Source: UniProt
  2. serine-type endopeptidase inhibitor activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of endopeptidase activity Source: RefGenome
  2. negative regulation of peptidase activity Source: UniProtKB
  3. regulation of proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Serpin B4
Alternative name(s):
Leupin
Peptidase inhibitor 11
Short name:
PI-11
Squamous cell carcinoma antigen 2
Short name:
SCCA-2
Gene namesi
Name:SERPINB4
Synonyms:PI11, SCCA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:10570. SERPINB4.

Subcellular locationi

Cytoplasm 1 Publication
Note: Seems to also be secreted in plasma by cancerous cells but at a low level.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular region Source: RefGenome
  3. extracellular space Source: InterPro
  4. extracellular vesicular exosome Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35539.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Serpin B4PRO_0000094104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP48594.
PRIDEiP48594.

2D gel databases

UCD-2DPAGEP48594.

PTM databases

PhosphoSiteiP48594.

Expressioni

Tissue specificityi

Squamous cells.

Gene expression databases

BgeeiP48594.
CleanExiHS_SERPINB4.
ExpressionAtlasiP48594. baseline.
GenevestigatoriP48594.

Interactioni

Protein-protein interaction databases

BioGridi112224. 23 interactions.
IntActiP48594. 6 interactions.
STRINGi9606.ENSP00000343445.

Structurei

3D structure databases

ProteinModelPortaliP48594.
SMRiP48594. Positions 2-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family. Ov-serpin subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP48594.
KOiK13963.
OMAiKGEDLSM.
OrthoDBiEOG7327PB.
PhylomeDBiP48594.
TreeFamiTF352619.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48594-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSLSEANTK FMFDLFQQFR KSKENNIFYS PISITSALGM VLLGAKDNTA
60 70 80 90 100
QQISKVLHFD QVTENTTEKA ATYHVDRSGN VHHQFQKLLT EFNKSTDAYE
110 120 130 140 150
LKIANKLFGE KTYQFLQEYL DAIKKFYQTS VESTDFANAP EESRKKINSW
160 170 180 190 200
VESQTNEKIK NLFPDGTIGN DTTLVLVNAI YFKGQWENKF KKENTKEEKF
210 220 230 240 250
WPNKNTYKSV QMMRQYNSFN FALLEDVQAK VLEIPYKGKD LSMIVLLPNE
260 270 280 290 300
IDGLQKLEEK LTAEKLMEWT SLQNMRETCV DLHLPRFKME ESYDLKDTLR
310 320 330 340 350
TMGMVNIFNG DADLSGMTWS HGLSVSKVLH KAFVEVTEEG VEAAAATAVV
360 370 380 390
VVELSSPSTN EEFCCNHPFL FFIRQNKTNS ILFYGRFSSP
Length:390
Mass (Da):44,854
Last modified:October 1, 1996 - v2
Checksum:i04E213CD892587D5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti353 – 3531E → K in BAF84901. (PubMed:14702039)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89015 mRNA. Translation: CAA61420.1.
U19557 mRNA. Translation: AAA97553.1.
U19576
, U19570, U19571, U19572, U19574, U19575 Genomic DNA. Translation: AAA92602.1.
AB035089 Genomic DNA. Translation: BAB21525.1.
AK292212 mRNA. Translation: BAF84901.1.
BC017401 mRNA. Translation: AAH17401.1.
CCDSiCCDS11986.1.
PIRiI38202.
RefSeqiNP_002965.1. NM_002974.3.
UniGeneiHs.123035.

Genome annotation databases

EnsembliENST00000341074; ENSP00000343445; ENSG00000206073.
GeneIDi6318.
KEGGihsa:6318.
UCSCiuc002ljf.3. human.

Polymorphism databases

DMDMi1710877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89015 mRNA. Translation: CAA61420.1 .
U19557 mRNA. Translation: AAA97553.1 .
U19576
, U19570 , U19571 , U19572 , U19574 , U19575 Genomic DNA. Translation: AAA92602.1 .
AB035089 Genomic DNA. Translation: BAB21525.1 .
AK292212 mRNA. Translation: BAF84901.1 .
BC017401 mRNA. Translation: AAH17401.1 .
CCDSi CCDS11986.1.
PIRi I38202.
RefSeqi NP_002965.1. NM_002974.3.
UniGenei Hs.123035.

3D structure databases

ProteinModelPortali P48594.
SMRi P48594. Positions 2-390.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112224. 23 interactions.
IntActi P48594. 6 interactions.
STRINGi 9606.ENSP00000343445.

Protein family/group databases

MEROPSi I04.008.

PTM databases

PhosphoSitei P48594.

Polymorphism databases

DMDMi 1710877.

2D gel databases

UCD-2DPAGE P48594.

Proteomic databases

MaxQBi P48594.
PRIDEi P48594.

Protocols and materials databases

DNASUi 6318.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000341074 ; ENSP00000343445 ; ENSG00000206073 .
GeneIDi 6318.
KEGGi hsa:6318.
UCSCi uc002ljf.3. human.

Organism-specific databases

CTDi 6318.
GeneCardsi GC18M061277.
HGNCi HGNC:10570. SERPINB4.
MIMi 600518. gene.
neXtProti NX_P48594.
PharmGKBi PA35539.
GenAtlasi Search...

Phylogenomic databases

GeneTreei ENSGT00760000118789.
HOGENOMi HOG000238519.
HOVERGENi HBG005957.
InParanoidi P48594.
KOi K13963.
OMAi KGEDLSM.
OrthoDBi EOG7327PB.
PhylomeDBi P48594.
TreeFami TF352619.

Miscellaneous databases

ChiTaRSi SERPINB4. human.
GeneWikii SERPINB4.
GenomeRNAii 6318.
NextBioi 24516.
PROi P48594.
SOURCEi Search...

Gene expression databases

Bgeei P48594.
CleanExi HS_SERPINB4.
ExpressionAtlasi P48594. baseline.
Genevestigatori P48594.

Family and domain databases

InterProi IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view ]
PANTHERi PTHR11461. PTHR11461. 1 hit.
Pfami PF00079. Serpin. 1 hit.
[Graphical view ]
SMARTi SM00093. SERPIN. 1 hit.
[Graphical view ]
SUPFAMi SSF56574. SSF56574. 1 hit.
PROSITEi PS00284. SERPIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel human serpin gene; cloning sequencing and expression of leupin."
    Barnes R.C., Worrall D.M.
    FEBS Lett. 373:61-65(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A serine proteinase inhibitor locus at 18q21.3 contains a tandem duplication of the human squamous cell carcinoma antigen gene."
    Schneider S.S., Schick C., Fish K.E., Miller E., Pena J.C., Treter S.D., Hui S.M., Silverman G.A.
    Proc. Natl. Acad. Sci. U.S.A. 92:3147-3151(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter."
    Hamada K., Shinomiya H., Asano Y., Kihana T., Iwamoto M., Hanakawa Y., Hashimoto K., Hirose S., Kyo S., Ito M.
    Biochim. Biophys. Acta 1518:124-131(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Esophagus.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow.
  6. Bienvenut W.V., Bensaad K., Vousden K.H.
    Submitted (FEB-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-21; 56-69; 88-94; 147-160; 215-260; 266-276 AND 378-386, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Osteosarcoma.
  7. "Circulating serpin tumor markers SCCA1 and SCCA2 are not actively secreted but reside in the cytosol of squamous carcinoma cells."
    Uemura Y., Pak S.C., Luke C., Cataltepe S., Tsu C., Schick C., Kamachi Y., Pomeroy S.L., Perlmutter D.H., Silverman G.A.
    Int. J. Cancer 89:368-377(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSPB4_HUMAN
AccessioniPrimary (citable) accession number: P48594
Secondary accession number(s): A8K847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3