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Protein

Serpin B4

Gene

SERPINB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a protease inhibitor to modulate the host immune response against tumor cells.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei354 – 3552Reactive bond

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • protease binding Source: UniProtKB
  • serine-type endopeptidase inhibitor activity Source: UniProtKB

GO - Biological processi

  • negative regulation of endopeptidase activity Source: GO_Central
  • negative regulation of peptidase activity Source: UniProtKB
  • protection from natural killer cell mediated cytotoxicity Source: UniProtKB
  • regulation of proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Serpin B4
Alternative name(s):
Leupin
Peptidase inhibitor 11
Short name:
PI-11
Squamous cell carcinoma antigen 2
Short name:
SCCA-2
Gene namesi
Name:SERPINB4
Synonyms:PI11, SCCA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:10570. SERPINB4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: GO_Central
  • intracellular Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35539.

Polymorphism and mutation databases

BioMutaiSERPINB4.
DMDMi1710877.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Serpin B4PRO_0000094104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP48594.
PRIDEiP48594.

2D gel databases

UCD-2DPAGEP48594.

PTM databases

PhosphoSiteiP48594.

Expressioni

Tissue specificityi

Squamous cells.

Gene expression databases

BgeeiP48594.
CleanExiHS_SERPINB4.
ExpressionAtlasiP48594. baseline and differential.
GenevisibleiP48594. HS.

Organism-specific databases

HPAiHPA048341.
HPA049988.
HPA055992.

Interactioni

Protein-protein interaction databases

BioGridi112224. 33 interactions.
IntActiP48594. 6 interactions.
STRINGi9606.ENSP00000343445.

Structurei

3D structure databases

ProteinModelPortaliP48594.
SMRiP48594. Positions 2-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family. Ov-serpin subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP48594.
KOiK13963.
OMAiKENTAQQ.
OrthoDBiEOG7327PB.
PhylomeDBiP48594.
TreeFamiTF352619.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSLSEANTK FMFDLFQQFR KSKENNIFYS PISITSALGM VLLGAKDNTA
60 70 80 90 100
QQISKVLHFD QVTENTTEKA ATYHVDRSGN VHHQFQKLLT EFNKSTDAYE
110 120 130 140 150
LKIANKLFGE KTYQFLQEYL DAIKKFYQTS VESTDFANAP EESRKKINSW
160 170 180 190 200
VESQTNEKIK NLFPDGTIGN DTTLVLVNAI YFKGQWENKF KKENTKEEKF
210 220 230 240 250
WPNKNTYKSV QMMRQYNSFN FALLEDVQAK VLEIPYKGKD LSMIVLLPNE
260 270 280 290 300
IDGLQKLEEK LTAEKLMEWT SLQNMRETCV DLHLPRFKME ESYDLKDTLR
310 320 330 340 350
TMGMVNIFNG DADLSGMTWS HGLSVSKVLH KAFVEVTEEG VEAAAATAVV
360 370 380 390
VVELSSPSTN EEFCCNHPFL FFIRQNKTNS ILFYGRFSSP
Length:390
Mass (Da):44,854
Last modified:October 1, 1996 - v2
Checksum:i04E213CD892587D5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti353 – 3531E → K in BAF84901 (PubMed:14702039).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89015 mRNA. Translation: CAA61420.1.
U19557 mRNA. Translation: AAA97553.1.
U19576
, U19570, U19571, U19572, U19574, U19575 Genomic DNA. Translation: AAA92602.1.
AB035089 Genomic DNA. Translation: BAB21525.1.
AK292212 mRNA. Translation: BAF84901.1.
BC017401 mRNA. Translation: AAH17401.1.
CCDSiCCDS11986.1.
PIRiI38202.
RefSeqiNP_002965.1. NM_002974.3.
NP_778206.1. NM_175041.1.
UniGeneiHs.123035.

Genome annotation databases

EnsembliENST00000341074; ENSP00000343445; ENSG00000206073.
GeneIDi6318.
KEGGihsa:6318.
UCSCiuc002ljf.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89015 mRNA. Translation: CAA61420.1.
U19557 mRNA. Translation: AAA97553.1.
U19576
, U19570, U19571, U19572, U19574, U19575 Genomic DNA. Translation: AAA92602.1.
AB035089 Genomic DNA. Translation: BAB21525.1.
AK292212 mRNA. Translation: BAF84901.1.
BC017401 mRNA. Translation: AAH17401.1.
CCDSiCCDS11986.1.
PIRiI38202.
RefSeqiNP_002965.1. NM_002974.3.
NP_778206.1. NM_175041.1.
UniGeneiHs.123035.

3D structure databases

ProteinModelPortaliP48594.
SMRiP48594. Positions 2-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112224. 33 interactions.
IntActiP48594. 6 interactions.
STRINGi9606.ENSP00000343445.

Protein family/group databases

MEROPSiI04.008.

PTM databases

PhosphoSiteiP48594.

Polymorphism and mutation databases

BioMutaiSERPINB4.
DMDMi1710877.

2D gel databases

UCD-2DPAGEP48594.

Proteomic databases

MaxQBiP48594.
PRIDEiP48594.

Protocols and materials databases

DNASUi6318.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000341074; ENSP00000343445; ENSG00000206073.
GeneIDi6318.
KEGGihsa:6318.
UCSCiuc002ljf.3. human.

Organism-specific databases

CTDi6318.
GeneCardsiGC18M061277.
HGNCiHGNC:10570. SERPINB4.
HPAiHPA048341.
HPA049988.
HPA055992.
MIMi600518. gene.
neXtProtiNX_P48594.
PharmGKBiPA35539.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00760000118789.
HOGENOMiHOG000238519.
HOVERGENiHBG005957.
InParanoidiP48594.
KOiK13963.
OMAiKENTAQQ.
OrthoDBiEOG7327PB.
PhylomeDBiP48594.
TreeFamiTF352619.

Miscellaneous databases

ChiTaRSiSERPINB4. human.
GeneWikiiSERPINB4.
GenomeRNAii6318.
NextBioi24516.
PROiP48594.
SOURCEiSearch...

Gene expression databases

BgeeiP48594.
CleanExiHS_SERPINB4.
ExpressionAtlasiP48594. baseline and differential.
GenevisibleiP48594. HS.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel human serpin gene; cloning sequencing and expression of leupin."
    Barnes R.C., Worrall D.M.
    FEBS Lett. 373:61-65(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A serine proteinase inhibitor locus at 18q21.3 contains a tandem duplication of the human squamous cell carcinoma antigen gene."
    Schneider S.S., Schick C., Fish K.E., Miller E., Pena J.C., Treter S.D., Hui S.M., Silverman G.A.
    Proc. Natl. Acad. Sci. U.S.A. 92:3147-3151(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter."
    Hamada K., Shinomiya H., Asano Y., Kihana T., Iwamoto M., Hanakawa Y., Hashimoto K., Hirose S., Kyo S., Ito M.
    Biochim. Biophys. Acta 1518:124-131(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Esophagus.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow.
  6. Bienvenut W.V., Bensaad K., Vousden K.H.
    Submitted (FEB-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-21; 56-69; 88-94; 147-160; 215-260; 266-276 AND 378-386, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Osteosarcoma.
  7. "Circulating serpin tumor markers SCCA1 and SCCA2 are not actively secreted but reside in the cytosol of squamous carcinoma cells."
    Uemura Y., Pak S.C., Luke C., Cataltepe S., Tsu C., Schick C., Kamachi Y., Pomeroy S.L., Perlmutter D.H., Silverman G.A.
    Int. J. Cancer 89:368-377(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSPB4_HUMAN
AccessioniPrimary (citable) accession number: P48594
Secondary accession number(s): A8K847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.