P48594 (SPB4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serpin B4

Alternative name(s):
Leupin
Peptidase inhibitor 11
Short name=PI-11
Squamous cell carcinoma antigen 2
Short name=SCCA-2

Gene names

Name:	SERPINB4
Synonyms:	PI11, SCCA2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a protease inhibitor to modulate the host immune response against tumor cells.
Subcellular location	Cytoplasm. Note: Seems to also be secreted in plasma by cancerous cells but at a low level. Ref.6
Tissue specificity	Squamous cells.
Sequence similarities	Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	immune response Non-traceable author statement. Source: UniProtKB regulation of proteolysis Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Non-traceable author statement. Source: UniProtKB extracellular region Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	serine-type endopeptidase inhibitor activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Serpin B4		PRO_0000094104
Sites
	Site	354 – 355	2	Reactive bond
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine

Sequences

	Sequence		Length	Mass (Da)
	P48594 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 04E213CD892587D5	FASTA	390	44,854
	10 20 30 40 50 60 MNSLSEANTK FMFDLFQQFR KSKENNIFYS PISITSALGM VLLGAKDNTA QQISKVLHFD 70 80 90 100 110 120 QVTENTTEKA ATYHVDRSGN VHHQFQKLLT EFNKSTDAYE LKIANKLFGE KTYQFLQEYL 130 140 150 160 170 180 DAIKKFYQTS VESTDFANAP EESRKKINSW VESQTNEKIK NLFPDGTIGN DTTLVLVNAI 190 200 210 220 230 240 YFKGQWENKF KKENTKEEKF WPNKNTYKSV QMMRQYNSFN FALLEDVQAK VLEIPYKGKD 250 260 270 280 290 300 LSMIVLLPNE IDGLQKLEEK LTAEKLMEWT SLQNMRETCV DLHLPRFKME ESYDLKDTLR 310 320 330 340 350 360 TMGMVNIFNG DADLSGMTWS HGLSVSKVLH KAFVEVTEEG VEAAAATAVV VVELSSPSTN 370 380 390 EEFCCNHPFL FFIRQNKTNS ILFYGRFSSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel human serpin gene; cloning sequencing and expression of leupin." Barnes R.C., Worrall D.M. FEBS Lett. 373:61-65(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A serine proteinase inhibitor locus at 18q21.3 contains a tandem duplication of the human squamous cell carcinoma antigen gene." Schneider S.S., Schick C., Fish K.E., Miller E., Pena J.C., Treter S.D., Hui S.M., Silverman G.A. Proc. Natl. Acad. Sci. U.S.A. 92:3147-3151(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter." Hamada K., Shinomiya H., Asano Y., Kihana T., Iwamoto M., Hanakawa Y., Hashimoto K., Hirose S., Kyo S., Ito M. Biochim. Biophys. Acta 1518:124-131(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[5]	Bienvenut W.V., Bensaad K., Vousden K.H. Submitted (FEB-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-21; 56-69; 88-94; 147-160; 215-260; 266-276 AND 378-386, MASS SPECTROMETRY. Tissue: Osteosarcoma.
[6]	"Circulating serpin tumor markers SCCA1 and SCCA2 are not actively secreted but reside in the cytosol of squamous carcinoma cells." Uemura Y., Pak S.C., Luke C., Cataltepe S., Tsu C., Schick C., Kamachi Y., Pomeroy S.L., Perlmutter D.H., Silverman G.A. Int. J. Cancer 89:368-377(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89015 mRNA. Translation: CAA61420.1. U19557 mRNA. Translation: AAA97553.1. U19576 , U19570, U19571, U19572, U19574, U19575 Genomic DNA. Translation: AAA92602.1. AB035089 Genomic DNA. Translation: BAB21525.1. BC017401 mRNA. Translation: AAH17401.1.
IPI	IPI00010303.
PIR	I38202.
RefSeq	NP_002965.1. NM_002974.2.
UniGene	Hs.123035.
3D structure databases
ProteinModelPortal	P48594.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48594. 4 interactions.
STRING	9606.ENSP00000343445.
Protein family/group databases
MEROPS	I04.008.
PTM databases
PhosphoSite	P48594.
Polymorphism databases
DMDM	1710877.
2D gel databases
UCD-2DPAGE	P48594.
Proteomic databases
PRIDE	P48594.
Protocols and materials databases
DNASU	6318.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000341074; ENSP00000343445; ENSG00000206073.
GeneID	6318.
KEGG	hsa:6318.
UCSC	uc002ljf.3. human.
Organism-specific databases
CTD	6318.
GeneCards	GC18M061277.
HGNC	HGNC:10570. SERPINB4.
HPA	CAB036006.
MIM	600518. gene.
neXtProt	NX_P48594.
PharmGKB	PA35539.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HOG000238519.
HOVERGEN	HBG005957.
InParanoid	P48594.
KO	K13963.
OMA	KSNDAYD.
OrthoDB	EOG47WNNX.
PhylomeDB	P48594.
Gene expression databases
ArrayExpress	P48594.
Bgee	P48594.
CleanEx	HS_SERPINB4.
Genevestigator	P48594.
GermOnline	ENSG00000057149. Homo sapiens.
Family and domain databases
InterPro	IPR023795. Protease_inhib_I4_serpin_CS. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SERPINB4. human.
GenomeRNAi	6318.
NextBio	24516.
SOURCE	Search...

Entry information

Entry name

SPB4_HUMAN

Accession

Primary (citable) accession number: P48594

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.