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P48594

- SPB4_HUMAN

UniProt

P48594 - SPB4_HUMAN

Protein

Serpin B4

Gene

SERPINB4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May act as a protease inhibitor to modulate the host immune response against tumor cells.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei354 – 3552Reactive bond

    GO - Molecular functioni

    1. enzyme binding Source: UniProt
    2. serine-type endopeptidase inhibitor activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of endopeptidase activity Source: RefGenome
    2. negative regulation of peptidase activity Source: UniProtKB
    3. regulation of proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI04.008.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serpin B4
    Alternative name(s):
    Leupin
    Peptidase inhibitor 11
    Short name:
    PI-11
    Squamous cell carcinoma antigen 2
    Short name:
    SCCA-2
    Gene namesi
    Name:SERPINB4
    Synonyms:PI11, SCCA2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:10570. SERPINB4.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Seems to also be secreted in plasma by cancerous cells but at a low level.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular region Source: RefGenome
    3. extracellular space Source: InterPro
    4. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35539.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Serpin B4PRO_0000094104Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP48594.
    PRIDEiP48594.

    2D gel databases

    UCD-2DPAGEP48594.

    PTM databases

    PhosphoSiteiP48594.

    Expressioni

    Tissue specificityi

    Squamous cells.

    Gene expression databases

    ArrayExpressiP48594.
    BgeeiP48594.
    CleanExiHS_SERPINB4.
    GenevestigatoriP48594.

    Interactioni

    Protein-protein interaction databases

    BioGridi112224. 9 interactions.
    IntActiP48594. 6 interactions.
    STRINGi9606.ENSP00000343445.

    Structurei

    3D structure databases

    ProteinModelPortaliP48594.
    SMRiP48594. Positions 2-390.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serpin family. Ov-serpin subfamily.Curated

    Phylogenomic databases

    HOGENOMiHOG000238519.
    HOVERGENiHBG005957.
    InParanoidiP48594.
    KOiK13963.
    OMAiKGEDLSM.
    OrthoDBiEOG7327PB.
    PhylomeDBiP48594.
    TreeFamiTF352619.

    Family and domain databases

    InterProiIPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 1 hit.
    PROSITEiPS00284. SERPIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48594-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSLSEANTK FMFDLFQQFR KSKENNIFYS PISITSALGM VLLGAKDNTA    50
    QQISKVLHFD QVTENTTEKA ATYHVDRSGN VHHQFQKLLT EFNKSTDAYE 100
    LKIANKLFGE KTYQFLQEYL DAIKKFYQTS VESTDFANAP EESRKKINSW 150
    VESQTNEKIK NLFPDGTIGN DTTLVLVNAI YFKGQWENKF KKENTKEEKF 200
    WPNKNTYKSV QMMRQYNSFN FALLEDVQAK VLEIPYKGKD LSMIVLLPNE 250
    IDGLQKLEEK LTAEKLMEWT SLQNMRETCV DLHLPRFKME ESYDLKDTLR 300
    TMGMVNIFNG DADLSGMTWS HGLSVSKVLH KAFVEVTEEG VEAAAATAVV 350
    VVELSSPSTN EEFCCNHPFL FFIRQNKTNS ILFYGRFSSP 390
    Length:390
    Mass (Da):44,854
    Last modified:October 1, 1996 - v2
    Checksum:i04E213CD892587D5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti353 – 3531E → K in BAF84901. (PubMed:14702039)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89015 mRNA. Translation: CAA61420.1.
    U19557 mRNA. Translation: AAA97553.1.
    U19576
    , U19570, U19571, U19572, U19574, U19575 Genomic DNA. Translation: AAA92602.1.
    AB035089 Genomic DNA. Translation: BAB21525.1.
    AK292212 mRNA. Translation: BAF84901.1.
    BC017401 mRNA. Translation: AAH17401.1.
    CCDSiCCDS11986.1.
    PIRiI38202.
    RefSeqiNP_002965.1. NM_002974.2.
    UniGeneiHs.123035.

    Genome annotation databases

    EnsembliENST00000341074; ENSP00000343445; ENSG00000206073.
    GeneIDi6318.
    KEGGihsa:6318.
    UCSCiuc002ljf.3. human.

    Polymorphism databases

    DMDMi1710877.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89015 mRNA. Translation: CAA61420.1 .
    U19557 mRNA. Translation: AAA97553.1 .
    U19576
    , U19570 , U19571 , U19572 , U19574 , U19575 Genomic DNA. Translation: AAA92602.1 .
    AB035089 Genomic DNA. Translation: BAB21525.1 .
    AK292212 mRNA. Translation: BAF84901.1 .
    BC017401 mRNA. Translation: AAH17401.1 .
    CCDSi CCDS11986.1.
    PIRi I38202.
    RefSeqi NP_002965.1. NM_002974.2.
    UniGenei Hs.123035.

    3D structure databases

    ProteinModelPortali P48594.
    SMRi P48594. Positions 2-390.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112224. 9 interactions.
    IntActi P48594. 6 interactions.
    STRINGi 9606.ENSP00000343445.

    Protein family/group databases

    MEROPSi I04.008.

    PTM databases

    PhosphoSitei P48594.

    Polymorphism databases

    DMDMi 1710877.

    2D gel databases

    UCD-2DPAGE P48594.

    Proteomic databases

    MaxQBi P48594.
    PRIDEi P48594.

    Protocols and materials databases

    DNASUi 6318.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000341074 ; ENSP00000343445 ; ENSG00000206073 .
    GeneIDi 6318.
    KEGGi hsa:6318.
    UCSCi uc002ljf.3. human.

    Organism-specific databases

    CTDi 6318.
    GeneCardsi GC18M061277.
    HGNCi HGNC:10570. SERPINB4.
    MIMi 600518. gene.
    neXtProti NX_P48594.
    PharmGKBi PA35539.
    GenAtlasi Search...

    Phylogenomic databases

    HOGENOMi HOG000238519.
    HOVERGENi HBG005957.
    InParanoidi P48594.
    KOi K13963.
    OMAi KGEDLSM.
    OrthoDBi EOG7327PB.
    PhylomeDBi P48594.
    TreeFami TF352619.

    Miscellaneous databases

    ChiTaRSi SERPINB4. human.
    GeneWikii SERPINB4.
    GenomeRNAii 6318.
    NextBioi 24516.
    PROi P48594.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48594.
    Bgeei P48594.
    CleanExi HS_SERPINB4.
    Genevestigatori P48594.

    Family and domain databases

    InterProi IPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 1 hit.
    PROSITEi PS00284. SERPIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel human serpin gene; cloning sequencing and expression of leupin."
      Barnes R.C., Worrall D.M.
      FEBS Lett. 373:61-65(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A serine proteinase inhibitor locus at 18q21.3 contains a tandem duplication of the human squamous cell carcinoma antigen gene."
      Schneider S.S., Schick C., Fish K.E., Miller E., Pena J.C., Treter S.D., Hui S.M., Silverman G.A.
      Proc. Natl. Acad. Sci. U.S.A. 92:3147-3151(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    3. "Molecular cloning of human squamous cell carcinoma antigen 1 gene and characterization of its promoter."
      Hamada K., Shinomiya H., Asano Y., Kihana T., Iwamoto M., Hanakawa Y., Hashimoto K., Hirose S., Kyo S., Ito M.
      Biochim. Biophys. Acta 1518:124-131(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Esophagus.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow.
    6. Bienvenut W.V., Bensaad K., Vousden K.H.
      Submitted (FEB-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1-21; 56-69; 88-94; 147-160; 215-260; 266-276 AND 378-386, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Osteosarcoma.
    7. "Circulating serpin tumor markers SCCA1 and SCCA2 are not actively secreted but reside in the cytosol of squamous carcinoma cells."
      Uemura Y., Pak S.C., Luke C., Cataltepe S., Tsu C., Schick C., Kamachi Y., Pomeroy S.L., Perlmutter D.H., Silverman G.A.
      Int. J. Cancer 89:368-377(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSPB4_HUMAN
    AccessioniPrimary (citable) accession number: P48594
    Secondary accession number(s): A8K847
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3