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P48592

- RIR2_DROME

UniProt

P48592 - RIR2_DROME

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Protein

Ribonucleoside-diphosphate reductase subunit M2

Gene
RnrS, CG8975
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi142 – 1421Iron 1 By similarity
Metal bindingi173 – 1731Iron 1 By similarity
Metal bindingi173 – 1731Iron 2 By similarity
Metal bindingi176 – 1761Iron 1 By similarity
Active sitei180 – 1801 By similarity
Metal bindingi236 – 2361Iron 2 By similarity
Metal bindingi270 – 2701Iron 2 By similarity
Metal bindingi273 – 2731Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: FlyBase
  2. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  3. DNA replication Source: UniProtKB-UniPathway
  4. neurogenesis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_180285. G1/S-Specific Transcription.
REACT_209388. E2F mediated regulation of DNA replication.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit M2 (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:RnrS
ORF Names:CG8975
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0011704. RnrS.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Ribonucleoside-diphosphate reductase subunit M2PRO_0000190454Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48592.

Expressioni

Gene expression databases

BgeeiP48592.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

BioGridi62064. 10 interactions.
DIPiDIP-18112N.
IntActiP48592. 4 interactions.
MINTiMINT-336377.
STRINGi7227.FBpp0087152.

Structurei

3D structure databases

ProteinModelPortaliP48592.
SMRiP48592. Positions 74-351.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
GeneTreeiENSGT00390000013305.
InParanoidiP48592.
KOiK10808.
OMAiGVMNSTK.
OrthoDBiEOG7VMP5N.
PhylomeDBiP48592.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48592-1 [UniParc]FASTAAdd to Basket

« Hide

MASKENIADN MEKFSLKSPS KKILTDSTNN VRKMSIGHEA NGQLAKESST    50
VNGIGKSANS LMEKSVTPFD PSLEPLLREN PRRFVIFPIQ YHDIWQMYKK 100
AEASFWTVEE VDLSKDLTDW HRLKDDERHF ISHVLAFFAA SDGIVNENLV 150
ERFSQEVQIT EARCFYGFQI AMENVHSEMY SVLIDTYIRD PHQREYLFNA 200
IETMPAVKRK ADWALSWISS KSANFGERII AFAAVEGIFF SGSFASIFWL 250
KKRGLMPGLT FSNELISRDE GLHCDFAVLM FQHLVQRPKR ERIIEIIRDA 300
VAIEQEFLTD ALPVNLIGMN CDLMSQYIEF VADRLLVELG VGKIYNTKNP 350
FNFMEMISLD GKTNFFEKKV GEYQRMGVVS NPLDNVFTLD ADF 393
Length:393
Mass (Da):45,115
Last modified:December 1, 2000 - v2
Checksum:i0784173BCF1F6D9A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013599 Genomic DNA. Translation: AAF58599.2.
AY051936 mRNA. Translation: AAK93360.1.
U09370 Genomic DNA. Translation: AAA56996.1.
RefSeqiNP_525111.1. NM_080372.3.
UniGeneiDm.569.

Genome annotation databases

EnsemblMetazoaiFBtr0088046; FBpp0087152; FBgn0011704.
GeneIDi36280.
KEGGidme:Dmel_CG8975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013599 Genomic DNA. Translation: AAF58599.2 .
AY051936 mRNA. Translation: AAK93360.1 .
U09370 Genomic DNA. Translation: AAA56996.1 .
RefSeqi NP_525111.1. NM_080372.3.
UniGenei Dm.569.

3D structure databases

ProteinModelPortali P48592.
SMRi P48592. Positions 74-351.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 62064. 10 interactions.
DIPi DIP-18112N.
IntActi P48592. 4 interactions.
MINTi MINT-336377.
STRINGi 7227.FBpp0087152.

Proteomic databases

PaxDbi P48592.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0088046 ; FBpp0087152 ; FBgn0011704 .
GeneIDi 36280.
KEGGi dme:Dmel_CG8975.

Organism-specific databases

CTDi 36280.
FlyBasei FBgn0011704. RnrS.

Phylogenomic databases

eggNOGi COG0208.
GeneTreei ENSGT00390000013305.
InParanoidi P48592.
KOi K10808.
OMAi GVMNSTK.
OrthoDBi EOG7VMP5N.
PhylomeDBi P48592.

Enzyme and pathway databases

UniPathwayi UPA00326 .
Reactomei REACT_180285. G1/S-Specific Transcription.
REACT_209388. E2F mediated regulation of DNA replication.

Miscellaneous databases

ChiTaRSi RnrS. drosophila.
GenomeRNAii 36280.
NextBioi 797712.
PROi P48592.

Gene expression databases

Bgeei P48592.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  4. "Developmental control of a G1-S transcriptional program in Drosophila."
    Duronio R.J., O'Farrell P.H.
    Development 120:1503-1515(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 149-362.
    Strain: Oregon-R.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiRIR2_DROME
AccessioniPrimary (citable) accession number: P48592
Secondary accession number(s): Q9V640
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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