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Protein

40S ribosomal protein S12

Gene

RPS12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-33837-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S121 Publication
Alternative name(s):
Small ribosomal subunit protein eS121 Publication
Gene namesi
Name:RPS121 Publication
Ordered Locus Names:YOR369C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR369C
SGDiS000005896 RPS12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001223401 – 14340S ribosomal protein S12Add BLAST143

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki85Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki95Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki114Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP48589
PaxDbiP48589
PRIDEiP48589
TopDownProteomicsiP48589

PTM databases

CarbonylDBiP48589
iPTMnetiP48589

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi34752, 50 interactors
ComplexPortaliCPX-1599 40S cytosolic small ribosomal subunit
IntActiP48589, 10 interactors
MINTiP48589
STRINGi4932.YOR369C

Structurei

Secondary structure

1143
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 37Combined sources16
Beta strandi41 – 44Combined sources4
Helixi45 – 53Combined sources9
Beta strandi58 – 64Combined sources7
Helixi70 – 75Combined sources6
Turni77 – 79Combined sources3
Beta strandi88 – 92Combined sources5
Helixi94 – 99Combined sources6
Turni100 – 102Combined sources3
Beta strandi103 – 108Combined sources6
Beta strandi111 – 114Combined sources4
Beta strandi119 – 124Combined sources6
Helixi130 – 139Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10121-143[»]
3J6Yelectron microscopy6.10121-143[»]
3J77electron microscopy6.20121-143[»]
3J78electron microscopy6.30121-143[»]
4U3MX-ray3.00C2/c22-143[»]
4U3NX-ray3.20C2/c22-143[»]
4U3UX-ray2.90C2/c22-143[»]
4U4NX-ray3.10C2/c22-143[»]
4U4OX-ray3.60C2/c22-143[»]
4U4QX-ray3.00C2/c22-143[»]
4U4RX-ray2.80C2/c22-143[»]
4U4UX-ray3.00C2/c22-143[»]
4U4YX-ray3.20C2/c22-143[»]
4U4ZX-ray3.10C2/c22-143[»]
4U50X-ray3.20C2/c22-143[»]
4U51X-ray3.20C2/c22-143[»]
4U52X-ray3.00C2/c22-143[»]
4U53X-ray3.30C2/c22-143[»]
4U55X-ray3.20C2/c22-143[»]
4U56X-ray3.45C2/c22-143[»]
4U6FX-ray3.10C2/c22-143[»]
4V88X-ray3.00AM/CM1-143[»]
4V8Yelectron microscopy4.30AM1-143[»]
4V8Zelectron microscopy6.60AM1-143[»]
4V92electron microscopy3.70M24-143[»]
5DATX-ray3.15C2/c220-142[»]
5DC3X-ray3.25C2/c22-143[»]
5DGEX-ray3.45C2/c22-143[»]
5DGVX-ray3.10C2/c22-143[»]
5FCIX-ray3.40C2/c22-143[»]
5FCJX-ray3.10C2/c22-143[»]
5I4LX-ray3.10C2/c220-143[»]
5JUOelectron microscopy4.00JB1-143[»]
5JUPelectron microscopy3.50JB1-143[»]
5JUSelectron microscopy4.20JB1-143[»]
5JUTelectron microscopy4.00JB1-143[»]
5JUUelectron microscopy4.00JB1-143[»]
5LYBX-ray3.25C220-143[»]
c225-143[»]
5M1Jelectron microscopy3.30M220-143[»]
5MC6electron microscopy3.80D1-143[»]
5MEIX-ray3.50N/c220-143[»]
5NDGX-ray3.70C2/c21-143[»]
5NDVX-ray3.30C2/c21-143[»]
5NDWX-ray3.70C2/c21-143[»]
5OBMX-ray3.40C2/c21-143[»]
5ON6X-ray3.10N/c220-143[»]
5TBWX-ray3.00N/c21-143[»]
5TGAX-ray3.30C2/c220-143[»]
5TGMX-ray3.50C2/c225-143[»]
5WYJelectron microscopy8.70SN1-143[»]
6EMLelectron microscopy3.60D1-143[»]
6FAIelectron microscopy3.40M1-143[»]
ProteinModelPortaliP48589
SMRiP48589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000018318
HOGENOMiHOG000210688
InParanoidiP48589
KOiK02951
OMAiGLRECAK
OrthoDBiEOG092C5G05

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
InterProiView protein in InterPro
IPR029064 L30e-like
IPR004038 Ribosomal_L7Ae/L30e/S12e/Gad45
IPR000530 Ribosomal_S12e
PANTHERiPTHR11843 PTHR11843, 1 hit
PfamiView protein in Pfam
PF01248 Ribosomal_L7Ae, 1 hit
PRINTSiPR00972 RIBSOMALS12E
SUPFAMiSSF55315 SSF55315, 1 hit
PROSITEiView protein in PROSITE
PS01189 RIBOSOMAL_S12E, 1 hit

Sequencei

Sequence statusi: Complete.

P48589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDVEEVVEV QEETVVEQTA EVTIEDALKV VLRTALVHDG LARGLRESTK
60 70 80 90 100
ALTRGEALLV VLVSSVTEAN IIKLVEGLAN DPENKVPLIK VADAKQLGEW
110 120 130 140
AGLGKIDREG NARKVVGASV VVVKNWGAET DELSMIMEHF SQQ
Length:143
Mass (Da):15,472
Last modified:February 1, 1996 - v1
Checksum:i7BD0434EB06A26B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90 – 92KVA → EGLP in AAA69926 (Ref. 2) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37460 Genomic DNA Translation: AAA80546.1
U01049 Genomic DNA Translation: AAA69926.1
Z75277 Genomic DNA Translation: CAA99700.1
U24143 mRNA Translation: AAA65439.1 Sequence problems.
BK006948 Genomic DNA Translation: DAA11128.1
PIRiS62102
RefSeqiNP_015014.3, NM_001183789.3

Genome annotation databases

EnsemblFungiiYOR369C; YOR369C; YOR369C
GeneIDi854551
KEGGisce:YOR369C

Similar proteinsi

Entry informationi

Entry nameiRS12_YEAST
AccessioniPrimary (citable) accession number: P48589
Secondary accession number(s): D6W362, Q02545
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 20, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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