Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

40S ribosomal protein S12

Gene

RPS12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-33837-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S12
Gene namesi
Name:RPS12
Ordered Locus Names:YOR369C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR369C.
SGDiS000005896. RPS12.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14314340S ribosomal protein S12PRO_0000122340Add
BLAST

Proteomic databases

MaxQBiP48589.
PeptideAtlasiP48589.
TopDownProteomicsiP48589.

PTM databases

iPTMnetiP48589.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi34752. 33 interactions.
IntActiP48589. 9 interactions.
MINTiMINT-1325368.

Structurei

Secondary structure

1
143
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 3712Combined sources
Beta strandi41 – 444Combined sources
Helixi45 – 506Combined sources
Turni51 – 566Combined sources
Beta strandi58 – 614Combined sources
Beta strandi67 – 693Combined sources
Helixi70 – 789Combined sources
Beta strandi82 – 843Combined sources
Helixi94 – 1018Combined sources
Beta strandi107 – 1104Combined sources
Beta strandi121 – 1233Combined sources
Helixi131 – 14010Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10121-143[»]
3J6Yelectron microscopy6.10121-143[»]
3J77electron microscopy6.20121-143[»]
3J78electron microscopy6.30121-143[»]
3V88X-ray3.00M1-143[»]
4U3MX-ray3.00C2/c22-143[»]
4U3NX-ray3.20C2/c22-143[»]
4U3UX-ray2.90C2/c22-143[»]
4U4NX-ray3.10C2/c22-143[»]
4U4OX-ray3.60C2/c22-143[»]
4U4QX-ray3.00C2/c22-143[»]
4U4RX-ray2.80C2/c22-143[»]
4U4UX-ray3.00C2/c22-143[»]
4U4YX-ray3.20C2/c22-143[»]
4U4ZX-ray3.10C2/c22-143[»]
4U50X-ray3.20C2/c22-143[»]
4U51X-ray3.20C2/c22-143[»]
4U52X-ray3.00C2/c22-143[»]
4U53X-ray3.30C2/c22-143[»]
4U55X-ray3.20C2/c22-143[»]
4U56X-ray3.45C2/c22-143[»]
4U6FX-ray3.10C2/c22-143[»]
4V88X-ray3.00AM/CM1-143[»]
4V8Yelectron microscopy4.30AM1-143[»]
4V8Zelectron microscopy6.60AM1-143[»]
4V92electron microscopy3.70M24-143[»]
ProteinModelPortaliP48589.
SMRiP48589. Positions 20-143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S12e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018318.
HOGENOMiHOG000210688.
InParanoidiP48589.
KOiK02951.
OMAiDAVRIVI.
OrthoDBiEOG73RBQC.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR000530. Ribosomal_S12e.
[Graphical view]
PANTHERiPTHR11843. PTHR11843. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00972. RIBSOMALS12E.
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS01189. RIBOSOMAL_S12E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDVEEVVEV QEETVVEQTA EVTIEDALKV VLRTALVHDG LARGLRESTK
60 70 80 90 100
ALTRGEALLV VLVSSVTEAN IIKLVEGLAN DPENKVPLIK VADAKQLGEW
110 120 130 140
AGLGKIDREG NARKVVGASV VVVKNWGAET DELSMIMEHF SQQ
Length:143
Mass (Da):15,472
Last modified:February 1, 1996 - v1
Checksum:i7BD0434EB06A26B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 923KVA → EGLP in AAA69926 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37460 Genomic DNA. Translation: AAA80546.1.
U01049 Genomic DNA. Translation: AAA69926.1.
Z75277 Genomic DNA. Translation: CAA99700.1.
U24143 mRNA. Translation: AAA65439.1. Sequence problems.
BK006948 Genomic DNA. Translation: DAA11128.1.
PIRiS62102.
RefSeqiNP_015014.3. NM_001183789.3.

Genome annotation databases

EnsemblFungiiYOR369C; YOR369C; YOR369C.
GeneIDi854551.
KEGGisce:YOR369C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37460 Genomic DNA. Translation: AAA80546.1.
U01049 Genomic DNA. Translation: AAA69926.1.
Z75277 Genomic DNA. Translation: CAA99700.1.
U24143 mRNA. Translation: AAA65439.1. Sequence problems.
BK006948 Genomic DNA. Translation: DAA11128.1.
PIRiS62102.
RefSeqiNP_015014.3. NM_001183789.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10121-143[»]
3J6Yelectron microscopy6.10121-143[»]
3J77electron microscopy6.20121-143[»]
3J78electron microscopy6.30121-143[»]
3V88X-ray3.00M1-143[»]
4U3MX-ray3.00C2/c22-143[»]
4U3NX-ray3.20C2/c22-143[»]
4U3UX-ray2.90C2/c22-143[»]
4U4NX-ray3.10C2/c22-143[»]
4U4OX-ray3.60C2/c22-143[»]
4U4QX-ray3.00C2/c22-143[»]
4U4RX-ray2.80C2/c22-143[»]
4U4UX-ray3.00C2/c22-143[»]
4U4YX-ray3.20C2/c22-143[»]
4U4ZX-ray3.10C2/c22-143[»]
4U50X-ray3.20C2/c22-143[»]
4U51X-ray3.20C2/c22-143[»]
4U52X-ray3.00C2/c22-143[»]
4U53X-ray3.30C2/c22-143[»]
4U55X-ray3.20C2/c22-143[»]
4U56X-ray3.45C2/c22-143[»]
4U6FX-ray3.10C2/c22-143[»]
4V88X-ray3.00AM/CM1-143[»]
4V8Yelectron microscopy4.30AM1-143[»]
4V8Zelectron microscopy6.60AM1-143[»]
4V92electron microscopy3.70M24-143[»]
ProteinModelPortaliP48589.
SMRiP48589. Positions 20-143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34752. 33 interactions.
IntActiP48589. 9 interactions.
MINTiMINT-1325368.

PTM databases

iPTMnetiP48589.

Proteomic databases

MaxQBiP48589.
PeptideAtlasiP48589.
TopDownProteomicsiP48589.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR369C; YOR369C; YOR369C.
GeneIDi854551.
KEGGisce:YOR369C.

Organism-specific databases

EuPathDBiFungiDB:YOR369C.
SGDiS000005896. RPS12.

Phylogenomic databases

GeneTreeiENSGT00390000018318.
HOGENOMiHOG000210688.
InParanoidiP48589.
KOiK02951.
OMAiDAVRIVI.
OrthoDBiEOG73RBQC.

Enzyme and pathway databases

BioCyciYEAST:G3O-33837-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

NextBioi976970.
PROiP48589.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR000530. Ribosomal_S12e.
[Graphical view]
PANTHERiPTHR11843. PTHR11843. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00972. RIBSOMALS12E.
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS01189. RIBOSOMAL_S12E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae."
    Siede W., Nusspaumer G., Portillo V., Rodriguez R., Friedberg E.C.
    Nucleic Acids Res. 24:1669-1675(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "Yeast homologue of ribosomal protein S12."
    Teply R.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 44774 / DBY747.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Moore J., Jacobs H.T., Kaiser K.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 29-126.
    Strain: ATCC 204660 / DBY746.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRS12_YEAST
AccessioniPrimary (citable) accession number: P48589
Secondary accession number(s): D6W362, Q02545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.