P48589 (RS12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S12

Gene names

Name:	RPS12
Ordered Locus Names:	YOR369C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribosomal protein S12e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Inferred by curator. Source: SGD
Cellular component	cytosolic small ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 143	143	40S ribosomal protein S12		PRO_0000122340
Amino acid modifications
	Modified residue	64	1	Phosphoserine Ref.7
	Modified residue	65	1	Phosphoserine Ref.7 Ref.8
	Modified residue	119	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	90 – 92	3	KVA → EGLP in AAA69926. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P48589 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7BD0434EB06A26B3	FASTA	143	15,472
	10 20 30 40 50 60 MSDVEEVVEV QEETVVEQTA EVTIEDALKV VLRTALVHDG LARGLRESTK ALTRGEALLV 70 80 90 100 110 120 VLVSSVTEAN IIKLVEGLAN DPENKVPLIK VADAKQLGEW AGLGKIDREG NARKVVGASV 130 140 VVVKNWGAET DELSMIMEHF SQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae." Siede W., Nusspaumer G., Portillo V., Rodriguez R., Friedberg E.C. Nucleic Acids Res. 24:1669-1675(1996) [PubMed: 8649984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Yeast homologue of ribosomal protein S12." Teply R. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 44774 / DBY747.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	Moore J., Jacobs H.T., Kaiser K. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 29-126. Strain: ATCC 204660 / DBY746.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64 AND SER-65, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-119, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37460 Genomic DNA. Translation: AAA80546.1. U01049 Genomic DNA. Translation: AAA69926.1. Z75277 Genomic DNA. Translation: CAA99700.1. U24143 mRNA. Translation: AAA65439.1. Sequence problems. BK006948 Genomic DNA. Translation: DAA11128.1.
PIR	S62102.
RefSeq	NP_015014.1. NM_001183789.1.
3D structure databases
ProteinModelPortal	P48589.
SMR	P48589. Positions 13-142.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48589. 4 interactions.
MINT	MINT-1325368.
STRING	P48589.
Proteomic databases
PeptideAtlas	P48589.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR369C; YOR369C; YOR369C.
GeneID	854551.
KEGG	sce:YOR369C.
NMPDR	fig|4932.3.peg.6133.
Organism-specific databases
CYGD	YOR369c.
SGD	S000005896. RPS12.
Phylogenomic databases
eggNOG	fuNOG10041.
GeneTree	EFGT00050000004062.
HOGENOM	HBG319268.
OMA	CVLAENC.
OrthoDB	EOG4TB7MV.
Gene expression databases
ArrayExpress	P48589.
Genevestigator	P48589.
GermOnline	YOR369C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45. IPR000530. Ribosomal_S12e. [Graphical view]
KO	K02951.
PANTHER	PTHR11843. Ribosomal_S12e. 1 hit.
Pfam	PF01248. Ribosomal_L7Ae. 1 hit. [Graphical view]
PRINTS	PR00972. RIBSOMALS12E.
PROSITE	PS01189. RIBOSOMAL_S12E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976970.

Entry information

Entry name

RS12_YEAST

Accession

Primary (citable) accession number: P48589
Secondary accession number(s): D6W362, Q02545

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program