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Protein

Vacuolar-sorting protein BRO1

Gene

BRO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.6 Publications

GO - Molecular functioni

  • deubiquitinase activator activity Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • intralumenal vesicle formation Source: SGD
  • positive regulation of ubiquitin-specific protease activity Source: SGD
  • protein deubiquitination Source: SGD
  • protein localization to endosome Source: SGD
  • protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
  • response to nutrient Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD
  • vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33990-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-sorting protein BRO1
Alternative name(s):
Amino acid sensor-independent protein 6
BCK1-like resistance to osmotic shock protein 1
BRO domain-containing protein 1
Nitrogen permease inactivating protein 3
Vacuolar protein-sorting-associated protein 31
Gene namesi
Name:BRO1
Synonyms:ASI6, LPF2, NPI3, VPS31
Ordered Locus Names:YPL084W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL084W.
SGDiS000006005. BRO1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • endosome Source: SGD
  • endosome membrane Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002188721 – 844Vacuolar-sorting protein BRO1Add BLAST844

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei740PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48582.
PRIDEiP48582.

PTM databases

iPTMnetiP48582.

Interactioni

Subunit structurei

Interacts with DOA4 and SNF7.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DOA4P3257115EBI-3768,EBI-19840
RFU1Q080032EBI-3768,EBI-2353109
SNF7P399293EBI-3768,EBI-17554

Protein-protein interaction databases

BioGridi36097. 93 interactors.
DIPiDIP-2225N.
IntActiP48582. 11 interactors.
MINTiMINT-422465.

Structurei

Secondary structure

1844
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 29Combined sources12
Turni32 – 34Combined sources3
Helixi35 – 38Combined sources4
Helixi41 – 53Combined sources13
Helixi60 – 81Combined sources22
Helixi82 – 88Combined sources7
Beta strandi92 – 95Combined sources4
Beta strandi100 – 102Combined sources3
Beta strandi107 – 110Combined sources4
Helixi113 – 134Combined sources22
Turni135 – 137Combined sources3
Beta strandi138 – 140Combined sources3
Helixi141 – 160Combined sources20
Helixi167 – 169Combined sources3
Helixi171 – 193Combined sources23
Helixi199 – 201Combined sources3
Helixi203 – 225Combined sources23
Turni230 – 232Combined sources3
Beta strandi233 – 235Combined sources3
Helixi239 – 264Combined sources26
Helixi270 – 285Combined sources16
Helixi286 – 288Combined sources3
Turni290 – 295Combined sources6
Helixi298 – 317Combined sources20
Beta strandi318 – 320Combined sources3
Helixi330 – 332Combined sources3
Helixi344 – 366Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZB1X-ray1.95A/B1-387[»]
ProteinModelPortaliP48582.
SMRiP48582.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48582.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 400BRO1PROSITE-ProRule annotationAdd BLAST397

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili547 – 583Sequence analysisAdd BLAST37

Domaini

The coiled-coil domain is essential for MVB sorting.
The BRO1 domain may be involved in the binding to SNF7.

Sequence similaritiesi

Contains 1 BRO1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000095264.
InParanoidiP48582.
OMAiAQSYGEN.
OrthoDBiEOG092C1JX6.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPYLFDLKL KDTEKLDWKK GLSSYLKKSY GSSQWRTFYD EKATSELDHL
60 70 80 90 100
RNNANGELAP SSLSEQNLKY YSFLEHLYFR LGSKGSRLKM DFTWYDAEYS
110 120 130 140 150
SAQKGLKYTQ HTLAFEKSCT LFNIAVIFTQ IARENINEDY KNSIANLTKA
160 170 180 190 200
FSCFEYLSEN FLNSPSVDLQ SENTRFLANI CHAEAQELFV LKLLNDQISS
210 220 230 240 250
KQYTLISKLS RATCNLFQKC HDFMKEIDDD VAIYGEPKWK TTVTCKLHFY
260 270 280 290 300
KSLSAYYHGL HLEEENRVGE AIAFLDFSMQ QLISSLPFKT WLVEFIDFDG
310 320 330 340 350
FKETLEKKQK ELIKDNDFIY HESVPAVVQV DSIKALDAIK SPTWEKILEP
360 370 380 390 400
YMQDVANKCD SLYRGIIPLD VYEKESIYSE EKATLLRKQV EETETANLEY
410 420 430 440 450
SSFIEFTNLP RLLSDLEKQF SDGNIFSNTD TQGQLMRDQI QTWCKFIQTN
460 470 480 490 500
EFRDIEEQMN KIVFKRKQIL EILSALPNDQ KENVTKLKSS LVAASNSDEK
510 520 530 540 550
LFACVKPHIV EINLLNDNGK IWKKFDEFNR NTPPQPSLLD IDDTKNDKIL
560 570 580 590 600
ELLKQVKGHA EDLRTLKEER SRNLSELRDE INNDDITKLL IINKGKSDVE
610 620 630 640 650
LKDLFEVELE KFEPLSTRIE ATIYKQSSMI DDIKAKLDEI FHLSNFKDKS
660 670 680 690 700
SGEEKFLEDR KNFFDKLQEA VKSFSIFASD LPKGIEFYDS LFNMSRDLAE
710 720 730 740 750
RVRVAKQTED STANSPAPPL PPLDSKASVV GGPPLLPQKS AAFQSLSRQG
760 770 780 790 800
LNLGDQFQNL KISAGSDLPQ GPGIPPRTYE ASPYAATPTM AAPPVPPKQS
810 820 830 840
QEDMYDLRRR KAVENEEREL QENPTSFYNR PSVFDENMYS KYSS
Length:844
Mass (Da):97,276
Last modified:October 5, 2010 - v2
Checksum:i34DE0CDD2F4AE55E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti359C → Y in AAB07790 (PubMed:8649366).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37364 Genomic DNA. Translation: AAB07790.1.
U41849 Genomic DNA. Translation: AAB68255.1.
BK006949 Genomic DNA. Translation: DAA11349.1.
PIRiS61104.
RefSeqiNP_015241.1. NM_001183898.1.

Genome annotation databases

EnsemblFungiiYPL084W; YPL084W; YPL084W.
GeneIDi856021.
KEGGisce:YPL084W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37364 Genomic DNA. Translation: AAB07790.1.
U41849 Genomic DNA. Translation: AAB68255.1.
BK006949 Genomic DNA. Translation: DAA11349.1.
PIRiS61104.
RefSeqiNP_015241.1. NM_001183898.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZB1X-ray1.95A/B1-387[»]
ProteinModelPortaliP48582.
SMRiP48582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36097. 93 interactors.
DIPiDIP-2225N.
IntActiP48582. 11 interactors.
MINTiMINT-422465.

PTM databases

iPTMnetiP48582.

Proteomic databases

MaxQBiP48582.
PRIDEiP48582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL084W; YPL084W; YPL084W.
GeneIDi856021.
KEGGisce:YPL084W.

Organism-specific databases

EuPathDBiFungiDB:YPL084W.
SGDiS000006005. BRO1.

Phylogenomic databases

HOGENOMiHOG000095264.
InParanoidiP48582.
OMAiAQSYGEN.
OrthoDBiEOG092C1JX6.

Enzyme and pathway databases

BioCyciYEAST:G3O-33990-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP48582.
PROiP48582.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRO1_YEAST
AccessioniPrimary (citable) accession number: P48582
Secondary accession number(s): D6W3T3, Q02823
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.