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P48582

- BRO1_YEAST

UniProt

P48582 - BRO1_YEAST

Protein

Vacuolar-sorting protein BRO1

Gene

BRO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. intralumenal vesicle formation Source: SGD
    2. protein deubiquitination Source: SGD
    3. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
    4. response to nutrient Source: SGD
    5. ubiquitin-dependent protein catabolic process Source: SGD
    6. vacuolar transport Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33990-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar-sorting protein BRO1
    Alternative name(s):
    Amino acid sensor-independent protein 6
    BCK1-like resistance to osmotic shock protein 1
    BRO domain-containing protein 1
    Nitrogen permease inactivating protein 3
    Vacuolar protein-sorting-associated protein 31
    Gene namesi
    Name:BRO1
    Synonyms:ASI6, LPF2, NPI3, VPS31
    Ordered Locus Names:YPL084W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    SGDiS000006005. BRO1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. endosome Source: SGD
    3. endosome membrane Source: GOC

    Keywords - Cellular componenti

    Cytoplasm, Endosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 844844Vacuolar-sorting protein BRO1PRO_0000218872Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei740 – 7401Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP48582.
    PaxDbiP48582.
    PeptideAtlasiP48582.

    Expressioni

    Gene expression databases

    GenevestigatoriP48582.

    Interactioni

    Subunit structurei

    Interacts with DOA4 and SNF7.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DOA4P3257115EBI-3768,EBI-19840
    RFU1Q080032EBI-3768,EBI-2353109

    Protein-protein interaction databases

    BioGridi36097. 92 interactions.
    DIPiDIP-2225N.
    IntActiP48582. 11 interactions.
    MINTiMINT-422465.
    STRINGi4932.YPL084W.

    Structurei

    Secondary structure

    1
    844
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi18 – 2912
    Turni32 – 343
    Helixi35 – 384
    Helixi41 – 5313
    Helixi60 – 8122
    Helixi82 – 887
    Beta strandi92 – 954
    Beta strandi100 – 1023
    Beta strandi107 – 1104
    Helixi113 – 13422
    Turni135 – 1373
    Beta strandi138 – 1403
    Helixi141 – 16020
    Helixi167 – 1693
    Helixi171 – 19323
    Helixi199 – 2013
    Helixi203 – 22523
    Turni230 – 2323
    Beta strandi233 – 2353
    Helixi239 – 26426
    Helixi270 – 28516
    Helixi286 – 2883
    Turni290 – 2956
    Helixi298 – 31720
    Beta strandi318 – 3203
    Helixi330 – 3323
    Helixi344 – 36623

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZB1X-ray1.95A/B1-387[»]
    ProteinModelPortaliP48582.
    SMRiP48582. Positions 1-702.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP48582.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 400397BRO1PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili547 – 58337Sequence AnalysisAdd
    BLAST

    Domaini

    The coiled-coil domain is essential for MVB sorting.
    The BRO1 domain may be involved in the binding to SNF7.

    Sequence similaritiesi

    Contains 1 BRO1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG325528.
    HOGENOMiHOG000095264.
    OMAiEIDWIAP.
    OrthoDBiEOG74J9K0.

    Family and domain databases

    Gene3Di1.25.40.280. 1 hit.
    InterProiIPR025304. ALIX_V_dom.
    IPR004328. BRO1_dom.
    [Graphical view]
    PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
    PF03097. BRO1. 1 hit.
    [Graphical view]
    SMARTiSM01041. BRO1. 1 hit.
    [Graphical view]
    PROSITEiPS51180. BRO1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48582-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPYLFDLKL KDTEKLDWKK GLSSYLKKSY GSSQWRTFYD EKATSELDHL    50
    RNNANGELAP SSLSEQNLKY YSFLEHLYFR LGSKGSRLKM DFTWYDAEYS 100
    SAQKGLKYTQ HTLAFEKSCT LFNIAVIFTQ IARENINEDY KNSIANLTKA 150
    FSCFEYLSEN FLNSPSVDLQ SENTRFLANI CHAEAQELFV LKLLNDQISS 200
    KQYTLISKLS RATCNLFQKC HDFMKEIDDD VAIYGEPKWK TTVTCKLHFY 250
    KSLSAYYHGL HLEEENRVGE AIAFLDFSMQ QLISSLPFKT WLVEFIDFDG 300
    FKETLEKKQK ELIKDNDFIY HESVPAVVQV DSIKALDAIK SPTWEKILEP 350
    YMQDVANKCD SLYRGIIPLD VYEKESIYSE EKATLLRKQV EETETANLEY 400
    SSFIEFTNLP RLLSDLEKQF SDGNIFSNTD TQGQLMRDQI QTWCKFIQTN 450
    EFRDIEEQMN KIVFKRKQIL EILSALPNDQ KENVTKLKSS LVAASNSDEK 500
    LFACVKPHIV EINLLNDNGK IWKKFDEFNR NTPPQPSLLD IDDTKNDKIL 550
    ELLKQVKGHA EDLRTLKEER SRNLSELRDE INNDDITKLL IINKGKSDVE 600
    LKDLFEVELE KFEPLSTRIE ATIYKQSSMI DDIKAKLDEI FHLSNFKDKS 650
    SGEEKFLEDR KNFFDKLQEA VKSFSIFASD LPKGIEFYDS LFNMSRDLAE 700
    RVRVAKQTED STANSPAPPL PPLDSKASVV GGPPLLPQKS AAFQSLSRQG 750
    LNLGDQFQNL KISAGSDLPQ GPGIPPRTYE ASPYAATPTM AAPPVPPKQS 800
    QEDMYDLRRR KAVENEEREL QENPTSFYNR PSVFDENMYS KYSS 844
    Length:844
    Mass (Da):97,276
    Last modified:October 5, 2010 - v2
    Checksum:i34DE0CDD2F4AE55E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti359 – 3591C → Y in AAB07790. (PubMed:8649366)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37364 Genomic DNA. Translation: AAB07790.1.
    U41849 Genomic DNA. Translation: AAB68255.1.
    BK006949 Genomic DNA. Translation: DAA11349.1.
    PIRiS61104.
    RefSeqiNP_015241.1. NM_001183898.1.

    Genome annotation databases

    EnsemblFungiiYPL084W; YPL084W; YPL084W.
    GeneIDi856021.
    KEGGisce:YPL084W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37364 Genomic DNA. Translation: AAB07790.1 .
    U41849 Genomic DNA. Translation: AAB68255.1 .
    BK006949 Genomic DNA. Translation: DAA11349.1 .
    PIRi S61104.
    RefSeqi NP_015241.1. NM_001183898.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZB1 X-ray 1.95 A/B 1-387 [» ]
    ProteinModelPortali P48582.
    SMRi P48582. Positions 1-702.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36097. 92 interactions.
    DIPi DIP-2225N.
    IntActi P48582. 11 interactions.
    MINTi MINT-422465.
    STRINGi 4932.YPL084W.

    Proteomic databases

    MaxQBi P48582.
    PaxDbi P48582.
    PeptideAtlasi P48582.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL084W ; YPL084W ; YPL084W .
    GeneIDi 856021.
    KEGGi sce:YPL084W.

    Organism-specific databases

    SGDi S000006005. BRO1.

    Phylogenomic databases

    eggNOGi NOG325528.
    HOGENOMi HOG000095264.
    OMAi EIDWIAP.
    OrthoDBi EOG74J9K0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33990-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P48582.
    NextBioi 980929.

    Gene expression databases

    Genevestigatori P48582.

    Family and domain databases

    Gene3Di 1.25.40.280. 1 hit.
    InterProi IPR025304. ALIX_V_dom.
    IPR004328. BRO1_dom.
    [Graphical view ]
    Pfami PF13949. ALIX_LYPXL_bnd. 1 hit.
    PF03097. BRO1. 1 hit.
    [Graphical view ]
    SMARTi SM01041. BRO1. 1 hit.
    [Graphical view ]
    PROSITEi PS51180. BRO1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "BRO1, a novel gene that interacts with components of the Pkc1p-mitogen-activated protein kinase pathway in Saccharomyces cerevisiae."
      Nickas M.E., Yaffe M.P.
      Mol. Cell. Biol. 16:2585-2593(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Suppressors of ssy1 and ptr3 null mutations define novel amino acid sensor-independent genes in Saccharomyces cerevisiae."
      Forsberg H., Hammar M., Andreasson C., Moliner A., Ljungdahl P.O.
      Genetics 158:973-988(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Yeast Npi3/Bro1 is involved in ubiquitin-dependent control of permease trafficking."
      Springael J.-Y., Nikko E., Andre B., Marini A.-M.
      FEBS Lett. 517:103-109(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae."
      Odorizzi G., Katzmann D.J., Babst M., Audhya A., Emr S.D.
      J. Cell Sci. 116:1893-1903(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway."
      Nikko E., Marini A.-M., Andre B.
      J. Biol. Chem. 278:50732-50743(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes."
      Luhtala N., Odorizzi G.
      J. Cell Biol. 166:717-729(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DOA4.
    11. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
      Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
      Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DOA4 AND SNF7.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-740, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBRO1_YEAST
    AccessioniPrimary (citable) accession number: P48582
    Secondary accession number(s): D6W3T3, Q02823
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3