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P48582 (BRO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar-sorting protein BRO1
Alternative name(s):
Amino acid sensor-independent protein 6
BCK1-like resistance to osmotic shock protein 1
BRO domain-containing protein 1
Nitrogen permease inactivating protein 3
Vacuolar protein-sorting-associated protein 31
Gene names
Name:BRO1
Synonyms:ASI6, LPF2, NPI3, VPS31
Ordered Locus Names:YPL084W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length844 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7 Ref.10

Subunit structure

Interacts with DOA4 and SNF7. Ref.10 Ref.11

Subcellular location

Cytoplasm. Endosome Ref.6 Ref.8 Ref.10.

Domain

The coiled-coil domain is essential for MVB sorting.

The BRO1 domain may be involved in the binding to SNF7.

Miscellaneous

Present with 10200 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 BRO1 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DOA4P3257115EBI-3768,EBI-19840
RFU1Q080032EBI-3768,EBI-2353109

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 844844Vacuolar-sorting protein BRO1
PRO_0000218872

Regions

Domain4 – 400397BRO1
Coiled coil547 – 58337 Potential

Amino acid modifications

Modified residue7401Phosphoserine Ref.12

Experimental info

Sequence conflict3591C → Y in AAB07790. Ref.1

Secondary structure

............................................... 844
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P48582 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 34DE0CDD2F4AE55E

FASTA84497,276
        10         20         30         40         50         60 
MKPYLFDLKL KDTEKLDWKK GLSSYLKKSY GSSQWRTFYD EKATSELDHL RNNANGELAP 

        70         80         90        100        110        120 
SSLSEQNLKY YSFLEHLYFR LGSKGSRLKM DFTWYDAEYS SAQKGLKYTQ HTLAFEKSCT 

       130        140        150        160        170        180 
LFNIAVIFTQ IARENINEDY KNSIANLTKA FSCFEYLSEN FLNSPSVDLQ SENTRFLANI 

       190        200        210        220        230        240 
CHAEAQELFV LKLLNDQISS KQYTLISKLS RATCNLFQKC HDFMKEIDDD VAIYGEPKWK 

       250        260        270        280        290        300 
TTVTCKLHFY KSLSAYYHGL HLEEENRVGE AIAFLDFSMQ QLISSLPFKT WLVEFIDFDG 

       310        320        330        340        350        360 
FKETLEKKQK ELIKDNDFIY HESVPAVVQV DSIKALDAIK SPTWEKILEP YMQDVANKCD 

       370        380        390        400        410        420 
SLYRGIIPLD VYEKESIYSE EKATLLRKQV EETETANLEY SSFIEFTNLP RLLSDLEKQF 

       430        440        450        460        470        480 
SDGNIFSNTD TQGQLMRDQI QTWCKFIQTN EFRDIEEQMN KIVFKRKQIL EILSALPNDQ 

       490        500        510        520        530        540 
KENVTKLKSS LVAASNSDEK LFACVKPHIV EINLLNDNGK IWKKFDEFNR NTPPQPSLLD 

       550        560        570        580        590        600 
IDDTKNDKIL ELLKQVKGHA EDLRTLKEER SRNLSELRDE INNDDITKLL IINKGKSDVE 

       610        620        630        640        650        660 
LKDLFEVELE KFEPLSTRIE ATIYKQSSMI DDIKAKLDEI FHLSNFKDKS SGEEKFLEDR 

       670        680        690        700        710        720 
KNFFDKLQEA VKSFSIFASD LPKGIEFYDS LFNMSRDLAE RVRVAKQTED STANSPAPPL 

       730        740        750        760        770        780 
PPLDSKASVV GGPPLLPQKS AAFQSLSRQG LNLGDQFQNL KISAGSDLPQ GPGIPPRTYE 

       790        800        810        820        830        840 
ASPYAATPTM AAPPVPPKQS QEDMYDLRRR KAVENEEREL QENPTSFYNR PSVFDENMYS 


KYSS 

« Hide

References

« Hide 'large scale' references
[1]"BRO1, a novel gene that interacts with components of the Pkc1p-mitogen-activated protein kinase pathway in Saccharomyces cerevisiae."
Nickas M.E., Yaffe M.P.
Mol. Cell. Biol. 16:2585-2593(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Suppressors of ssy1 and ptr3 null mutations define novel amino acid sensor-independent genes in Saccharomyces cerevisiae."
Forsberg H., Hammar M., Andreasson C., Moliner A., Ljungdahl P.O.
Genetics 158:973-988(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Yeast Npi3/Bro1 is involved in ubiquitin-dependent control of permease trafficking."
Springael J.-Y., Nikko E., Andre B., Marini A.-M.
FEBS Lett. 517:103-109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae."
Odorizzi G., Katzmann D.J., Babst M., Audhya A., Emr S.D.
J. Cell Sci. 116:1893-1903(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway."
Nikko E., Marini A.-M., Andre B.
J. Biol. Chem. 278:50732-50743(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes."
Luhtala N., Odorizzi G.
J. Cell Biol. 166:717-729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DOA4.
[11]"Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DOA4 AND SNF7.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-740, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37364 Genomic DNA. Translation: AAB07790.1.
U41849 Genomic DNA. Translation: AAB68255.1.
BK006949 Genomic DNA. Translation: DAA11349.1.
PIRS61104.
RefSeqNP_015241.1. NM_001183898.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZB1X-ray1.95A/B1-387[»]
ProteinModelPortalP48582.
SMRP48582. Positions 1-702.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36097. 92 interactions.
DIPDIP-2225N.
IntActP48582. 11 interactions.
MINTMINT-422465.
STRING4932.YPL084W.

Proteomic databases

PaxDbP48582.
PeptideAtlasP48582.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL084W; YPL084W; YPL084W.
GeneID856021.
KEGGsce:YPL084W.

Organism-specific databases

SGDS000006005. BRO1.

Phylogenomic databases

eggNOGNOG325528.
HOGENOMHOG000095264.
OMAKYFTSVA.
OrthoDBEOG74J9K0.

Enzyme and pathway databases

BioCycYEAST:G3O-33990-MONOMER.

Gene expression databases

GenevestigatorP48582.

Family and domain databases

Gene3D1.25.40.280. 1 hit.
InterProIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEPS51180. BRO1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP48582.
NextBio980929.

Entry information

Entry nameBRO1_YEAST
AccessionPrimary (citable) accession number: P48582
Secondary accession number(s): D6W3T3, Q02823
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 5, 2010
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references