Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P48582

- BRO1_YEAST

UniProt

P48582 - BRO1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Vacuolar-sorting protein BRO1

Gene

BRO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.6 Publications

GO - Biological processi

  1. intralumenal vesicle formation Source: SGD
  2. protein deubiquitination Source: SGD
  3. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
  4. response to nutrient Source: SGD
  5. ubiquitin-dependent protein catabolic process Source: SGD
  6. vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33990-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-sorting protein BRO1
Alternative name(s):
Amino acid sensor-independent protein 6
BCK1-like resistance to osmotic shock protein 1
BRO domain-containing protein 1
Nitrogen permease inactivating protein 3
Vacuolar protein-sorting-associated protein 31
Gene namesi
Name:BRO1
Synonyms:ASI6, LPF2, NPI3, VPS31
Ordered Locus Names:YPL084W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

SGDiS000006005. BRO1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. endosome Source: SGD
  3. endosome membrane Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 844844Vacuolar-sorting protein BRO1PRO_0000218872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei740 – 7401Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48582.
PaxDbiP48582.
PeptideAtlasiP48582.

Expressioni

Gene expression databases

GenevestigatoriP48582.

Interactioni

Subunit structurei

Interacts with DOA4 and SNF7.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DOA4P3257115EBI-3768,EBI-19840
RFU1Q080032EBI-3768,EBI-2353109

Protein-protein interaction databases

BioGridi36097. 93 interactions.
DIPiDIP-2225N.
IntActiP48582. 11 interactions.
MINTiMINT-422465.
STRINGi4932.YPL084W.

Structurei

Secondary structure

1
844
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 2912Combined sources
Turni32 – 343Combined sources
Helixi35 – 384Combined sources
Helixi41 – 5313Combined sources
Helixi60 – 8122Combined sources
Helixi82 – 887Combined sources
Beta strandi92 – 954Combined sources
Beta strandi100 – 1023Combined sources
Beta strandi107 – 1104Combined sources
Helixi113 – 13422Combined sources
Turni135 – 1373Combined sources
Beta strandi138 – 1403Combined sources
Helixi141 – 16020Combined sources
Helixi167 – 1693Combined sources
Helixi171 – 19323Combined sources
Helixi199 – 2013Combined sources
Helixi203 – 22523Combined sources
Turni230 – 2323Combined sources
Beta strandi233 – 2353Combined sources
Helixi239 – 26426Combined sources
Helixi270 – 28516Combined sources
Helixi286 – 2883Combined sources
Turni290 – 2956Combined sources
Helixi298 – 31720Combined sources
Beta strandi318 – 3203Combined sources
Helixi330 – 3323Combined sources
Helixi344 – 36623Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZB1X-ray1.95A/B1-387[»]
ProteinModelPortaliP48582.
SMRiP48582. Positions 1-702.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48582.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 400397BRO1PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili547 – 58337Sequence AnalysisAdd
BLAST

Domaini

The coiled-coil domain is essential for MVB sorting.
The BRO1 domain may be involved in the binding to SNF7.

Sequence similaritiesi

Contains 1 BRO1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG325528.
GeneTreeiENSGT00770000120598.
HOGENOMiHOG000095264.
InParanoidiP48582.
OMAiEIDWIAP.
OrthoDBiEOG74J9K0.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48582-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPYLFDLKL KDTEKLDWKK GLSSYLKKSY GSSQWRTFYD EKATSELDHL
60 70 80 90 100
RNNANGELAP SSLSEQNLKY YSFLEHLYFR LGSKGSRLKM DFTWYDAEYS
110 120 130 140 150
SAQKGLKYTQ HTLAFEKSCT LFNIAVIFTQ IARENINEDY KNSIANLTKA
160 170 180 190 200
FSCFEYLSEN FLNSPSVDLQ SENTRFLANI CHAEAQELFV LKLLNDQISS
210 220 230 240 250
KQYTLISKLS RATCNLFQKC HDFMKEIDDD VAIYGEPKWK TTVTCKLHFY
260 270 280 290 300
KSLSAYYHGL HLEEENRVGE AIAFLDFSMQ QLISSLPFKT WLVEFIDFDG
310 320 330 340 350
FKETLEKKQK ELIKDNDFIY HESVPAVVQV DSIKALDAIK SPTWEKILEP
360 370 380 390 400
YMQDVANKCD SLYRGIIPLD VYEKESIYSE EKATLLRKQV EETETANLEY
410 420 430 440 450
SSFIEFTNLP RLLSDLEKQF SDGNIFSNTD TQGQLMRDQI QTWCKFIQTN
460 470 480 490 500
EFRDIEEQMN KIVFKRKQIL EILSALPNDQ KENVTKLKSS LVAASNSDEK
510 520 530 540 550
LFACVKPHIV EINLLNDNGK IWKKFDEFNR NTPPQPSLLD IDDTKNDKIL
560 570 580 590 600
ELLKQVKGHA EDLRTLKEER SRNLSELRDE INNDDITKLL IINKGKSDVE
610 620 630 640 650
LKDLFEVELE KFEPLSTRIE ATIYKQSSMI DDIKAKLDEI FHLSNFKDKS
660 670 680 690 700
SGEEKFLEDR KNFFDKLQEA VKSFSIFASD LPKGIEFYDS LFNMSRDLAE
710 720 730 740 750
RVRVAKQTED STANSPAPPL PPLDSKASVV GGPPLLPQKS AAFQSLSRQG
760 770 780 790 800
LNLGDQFQNL KISAGSDLPQ GPGIPPRTYE ASPYAATPTM AAPPVPPKQS
810 820 830 840
QEDMYDLRRR KAVENEEREL QENPTSFYNR PSVFDENMYS KYSS
Length:844
Mass (Da):97,276
Last modified:October 5, 2010 - v2
Checksum:i34DE0CDD2F4AE55E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti359 – 3591C → Y in AAB07790. (PubMed:8649366)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37364 Genomic DNA. Translation: AAB07790.1.
U41849 Genomic DNA. Translation: AAB68255.1.
BK006949 Genomic DNA. Translation: DAA11349.1.
PIRiS61104.
RefSeqiNP_015241.1. NM_001183898.1.

Genome annotation databases

EnsemblFungiiYPL084W; YPL084W; YPL084W.
GeneIDi856021.
KEGGisce:YPL084W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37364 Genomic DNA. Translation: AAB07790.1 .
U41849 Genomic DNA. Translation: AAB68255.1 .
BK006949 Genomic DNA. Translation: DAA11349.1 .
PIRi S61104.
RefSeqi NP_015241.1. NM_001183898.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZB1 X-ray 1.95 A/B 1-387 [» ]
ProteinModelPortali P48582.
SMRi P48582. Positions 1-702.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36097. 93 interactions.
DIPi DIP-2225N.
IntActi P48582. 11 interactions.
MINTi MINT-422465.
STRINGi 4932.YPL084W.

Proteomic databases

MaxQBi P48582.
PaxDbi P48582.
PeptideAtlasi P48582.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL084W ; YPL084W ; YPL084W .
GeneIDi 856021.
KEGGi sce:YPL084W.

Organism-specific databases

SGDi S000006005. BRO1.

Phylogenomic databases

eggNOGi NOG325528.
GeneTreei ENSGT00770000120598.
HOGENOMi HOG000095264.
InParanoidi P48582.
OMAi EIDWIAP.
OrthoDBi EOG74J9K0.

Enzyme and pathway databases

BioCyci YEAST:G3O-33990-MONOMER.

Miscellaneous databases

EvolutionaryTracei P48582.
NextBioi 980929.

Gene expression databases

Genevestigatori P48582.

Family and domain databases

Gene3Di 1.25.40.280. 1 hit.
InterProi IPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view ]
Pfami PF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view ]
SMARTi SM01041. BRO1. 1 hit.
[Graphical view ]
PROSITEi PS51180. BRO1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "BRO1, a novel gene that interacts with components of the Pkc1p-mitogen-activated protein kinase pathway in Saccharomyces cerevisiae."
    Nickas M.E., Yaffe M.P.
    Mol. Cell. Biol. 16:2585-2593(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Suppressors of ssy1 and ptr3 null mutations define novel amino acid sensor-independent genes in Saccharomyces cerevisiae."
    Forsberg H., Hammar M., Andreasson C., Moliner A., Ljungdahl P.O.
    Genetics 158:973-988(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Yeast Npi3/Bro1 is involved in ubiquitin-dependent control of permease trafficking."
    Springael J.-Y., Nikko E., Andre B., Marini A.-M.
    FEBS Lett. 517:103-109(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae."
    Odorizzi G., Katzmann D.J., Babst M., Audhya A., Emr S.D.
    J. Cell Sci. 116:1893-1903(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway."
    Nikko E., Marini A.-M., Andre B.
    J. Biol. Chem. 278:50732-50743(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes."
    Luhtala N., Odorizzi G.
    J. Cell Biol. 166:717-729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DOA4.
  11. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
    Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
    Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DOA4 AND SNF7.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-740, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBRO1_YEAST
AccessioniPrimary (citable) accession number: P48582
Secondary accession number(s): D6W3T3, Q02823
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 5, 2010
Last modified: November 26, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3