P48582 (BRO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar-sorting protein BRO1

Alternative name(s):
Amino acid sensor-independent protein 6
BCK1-like resistance to osmotic shock protein 1
BRO domain-containing protein 1
Nitrogen permease inactivating protein 3
Vacuolar protein-sorting-associated protein 31

Gene names

Name:	BRO1
Synonyms:	ASI6, LPF2, NPI3, VPS31
Ordered Locus Names:	YPL084W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	844 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7 Ref.10
Subunit structure	Interacts with DOA4 and SNF7. Ref.10 Ref.11
Subcellular location	Cytoplasm. Endosome Ref.6 Ref.8 Ref.10.
Domain	The coiled-coil domain is essential for MVB sorting. The BRO1 domain may be involved in the binding to SNF7.
Miscellaneous	Present with 10200 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 BRO1 domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from genetic interaction Ref.10. Source: SGD protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Inferred from mutant phenotype Ref.7. Source: SGD response to nutrient Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	endosome Inferred from direct assay Ref.6PubMed 15935782. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DOA4	P32571	3	EBI-3768,EBI-19840
RFU1	Q08003	2	EBI-3768,EBI-2353109

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 844

844

Vacuolar-sorting protein BRO1

PRO_0000218872

Regions

Domain

4 – 400

397

BRO1

Coiled coil

547 – 583

37

Potential

Amino acid modifications

Modified residue

728

1

Phosphoserine Ref.12

Modified residue

740

1

Phosphoserine Ref.12

Experimental info

Sequence conflict

359

1

C → Y in AAB07790. Ref.1

Secondary structure

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844

Helix Strand Turn

Details...

Helix

18 – 29

12

Turn

32 – 34

3

Helix

35 – 38

4

Helix

41 – 53

13

Helix

60 – 81

22

Helix

82 – 88

7

Beta strand

92 – 95

4

Beta strand

100 – 102

3

Beta strand

107 – 110

4

Helix

113 – 134

22

Turn

135 – 137

3

Beta strand

138 – 140

3

Helix

141 – 160

20

Helix

167 – 169

3

Helix

171 – 193

23

Helix

199 – 201

3

Helix

203 – 225

23

Turn

230 – 232

3

Beta strand

233 – 235

3

Helix

239 – 264

26

Helix

270 – 285

16

Helix

286 – 288

3

Turn

290 – 295

6

Helix

298 – 317

20

Beta strand

318 – 320

3

Helix

330 – 332

3

Helix

344 – 366

23

Sequences

	Sequence		Length	Mass (Da)
	P48582 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: 34DE0CDD2F4AE55E	FASTA	844	97,276
	10 20 30 40 50 60 MKPYLFDLKL KDTEKLDWKK GLSSYLKKSY GSSQWRTFYD EKATSELDHL RNNANGELAP 70 80 90 100 110 120 SSLSEQNLKY YSFLEHLYFR LGSKGSRLKM DFTWYDAEYS SAQKGLKYTQ HTLAFEKSCT 130 140 150 160 170 180 LFNIAVIFTQ IARENINEDY KNSIANLTKA FSCFEYLSEN FLNSPSVDLQ SENTRFLANI 190 200 210 220 230 240 CHAEAQELFV LKLLNDQISS KQYTLISKLS RATCNLFQKC HDFMKEIDDD VAIYGEPKWK 250 260 270 280 290 300 TTVTCKLHFY KSLSAYYHGL HLEEENRVGE AIAFLDFSMQ QLISSLPFKT WLVEFIDFDG 310 320 330 340 350 360 FKETLEKKQK ELIKDNDFIY HESVPAVVQV DSIKALDAIK SPTWEKILEP YMQDVANKCD 370 380 390 400 410 420 SLYRGIIPLD VYEKESIYSE EKATLLRKQV EETETANLEY SSFIEFTNLP RLLSDLEKQF 430 440 450 460 470 480 SDGNIFSNTD TQGQLMRDQI QTWCKFIQTN EFRDIEEQMN KIVFKRKQIL EILSALPNDQ 490 500 510 520 530 540 KENVTKLKSS LVAASNSDEK LFACVKPHIV EINLLNDNGK IWKKFDEFNR NTPPQPSLLD 550 560 570 580 590 600 IDDTKNDKIL ELLKQVKGHA EDLRTLKEER SRNLSELRDE INNDDITKLL IINKGKSDVE 610 620 630 640 650 660 LKDLFEVELE KFEPLSTRIE ATIYKQSSMI DDIKAKLDEI FHLSNFKDKS SGEEKFLEDR 670 680 690 700 710 720 KNFFDKLQEA VKSFSIFASD LPKGIEFYDS LFNMSRDLAE RVRVAKQTED STANSPAPPL 730 740 750 760 770 780 PPLDSKASVV GGPPLLPQKS AAFQSLSRQG LNLGDQFQNL KISAGSDLPQ GPGIPPRTYE 790 800 810 820 830 840 ASPYAATPTM AAPPVPPKQS QEDMYDLRRR KAVENEEREL QENPTSFYNR PSVFDENMYS KYSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BRO1, a novel gene that interacts with components of the Pkc1p-mitogen-activated protein kinase pathway in Saccharomyces cerevisiae." Nickas M.E., Yaffe M.P. Mol. Cell. Biol. 16:2585-2593(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Suppressors of ssy1 and ptr3 null mutations define novel amino acid sensor-independent genes in Saccharomyces cerevisiae." Forsberg H., Hammar M., Andreasson C., Moliner A., Ljungdahl P.O. Genetics 158:973-988(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Yeast Npi3/Bro1 is involved in ubiquitin-dependent control of permease trafficking." Springael J.-Y., Nikko E., Andre B., Marini A.-M. FEBS Lett. 517:103-109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae." Odorizzi G., Katzmann D.J., Babst M., Audhya A., Emr S.D. J. Cell Sci. 116:1893-1903(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway." Nikko E., Marini A.-M., Andre B. J. Biol. Chem. 278:50732-50743(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes." Luhtala N., Odorizzi G. J. Cell Biol. 166:717-729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DOA4.
[11]	"Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae." Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H. Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DOA4 AND SNF7.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-728 AND SER-740, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U37364 Genomic DNA. Translation: AAB07790.1.
U41849 Genomic DNA. Translation: AAB68255.1.
BK006949 Genomic DNA. Translation: DAA11349.1.

PIR

S61104.

RefSeq

NP_015241.1. NM_001183898.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZB1	X-ray	1.95	A/B	1-387	[»]

ProteinModelPortal

P48582.

SMR

P48582. Positions 1-691.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2225N.

IntAct

P48582. 6 interactions.

MINT

MINT-422465.

STRING

4932.YPL084W.

Proteomic databases

PaxDb

P48582.

PeptideAtlas

P48582.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YPL084W; YPL084W; YPL084W.

GeneID

856021.

KEGG

sce:YPL084W.

Organism-specific databases

SGD

S000006005. BRO1.

Phylogenomic databases

eggNOG

NOG325528.

GeneTree

ENSGT00670000098036.

HOGENOM

HOG000095264.

OMA

ADWEYQS.

OrthoDB

EOG4GBBF9.

Enzyme and pathway databases

BioCyc

YEAST:G3O-33990-MONOMER.

Gene expression databases

Genevestigator

P48582.

GermOnline

YPL084W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.25.40.280. 1 hit.

InterPro

IPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]

Pfam

PF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]

SMART

SM01041. BRO1. 1 hit.
[Graphical view]

PROSITE

PS51180. BRO1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P48582.

NextBio

980929.

Entry information

Entry name

BRO1_YEAST

Accession

Primary (citable) accession number: P48582
Secondary accession number(s): D6W3T3, Q02823

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 5, 2010
Last modified:	May 29, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program