P48581 (RAD17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA damage checkpoint control protein RAD17

Alternative name(s):
DNA repair exonuclease RAD17

Gene names

Name:	RAD17
Ordered Locus Names:	YOR368W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the checkpoint clamp complex involved in the surveillance mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes. Associates with sites of DNA damage and modulates the MEC1 signaling pathway and the activation of RAD53 in response to DNA damage at phase G1. The complex also physically regulates DNA polymerase zeta-dependent mutagenesis by controlling the access of polymerase zeta to damaged DNA. Contrary to its human counterpart, the 9-1-1 complex, the checkpoint clamp complex shows no detectable exonuclease activity. Ref.1 Ref.2 Ref.5 Ref.6 Ref.7 Ref.8 Ref.11 Ref.12
Subunit structure	Component of the checkpoint clamp complex composed of DDC1, MEC3 and RAD17. The interaction with MEC3 is performed in a RAD17-dependent manner. The checkpoint clamp complex loads onto DNA. Interacts with the DNA polymerase zeta subunit REV7. 2 RAD17 subunits form also an hetero trimer with one MEC3 subunit. Ref.6 Ref.10 Ref.12 Ref.13
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 189 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the rad1 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA damage checkpoint Inferred from mutant phenotype. Source: SGD double-strand break repair Inferred from mutant phenotype. Source: SGD reciprocal meiotic recombination Inferred from mutant phenotype. Source: SGD
Cellular component	checkpoint clamp complex Inferred from direct assay Ref.6Ref.7. Source: SGD
Molecular function	damaged DNA binding Inferred from electronic annotation. Source: InterPro double-stranded DNA binding Inferred from direct assay Ref.6. Source: SGD exodeoxyribonuclease III activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.7. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DDC1	Q08949	4	EBI-14652,EBI-30769
MEC3	Q02574	9	EBI-14652,EBI-10658

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	DNA damage checkpoint control protein RAD17		PRO_0000097150
Amino acid modifications
	Modified residue	5	1	Phosphoserine Ref.16
	Modified residue	357	1	Phosphoserine Ref.15 Ref.16
	Modified residue	383	1	Phosphoserine Ref.14
Experimental info
	Mutagenesis	128	1	E → K in RAD17-1; UV-sensitive. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48581 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: BFF7074676C483C9	FASTA	401	45,580
	10 20 30 40 50 60 MRINSELANK FSASTVHLEH ITTALSCLTP FGSKDDVLIF IDADGLSFVR ENNHVIKIQL 70 80 90 100 110 120 LLSRELFMSY SYRNETEDHM KLCVKINHIL DSVSVMNRNS DDIVECTLSY DGHGSPFVLI 130 140 150 160 170 180 FEDSFISERV EYSTYLIKDF DTNGLELDRE RISFEAIIKG EALHSALKDL KEIGCKECYV 190 200 210 220 230 240 YAKTEANDEN VFALISKSQL GFSKIKLPSN RSILEKLQVF DGDSTTVIDG FAVIGFFDFT 250 260 270 280 290 300 SFDKIRKSTK IASKVLFRMD VHGVLSVNIL SQTDDVIITD TTRPSNNRPG SIRQLQLPKD 310 320 330 340 350 360 YPGIVIEVCM LEKESIDEAA QTEIELLMET NELGNRNSFK KSTIRKRYGT DKGNETSNDN 370 380 390 400 LLQLNGKKIK LPSEEENNKN RESEDEENHC KYPTKDIPIF F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae." Siede W., Nusspaumer G., Portillo V., Rodriguez R., Friedberg E.C. Nucleic Acids Res. 24:1669-1675(1996) [PubMed: 8649984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF GLU-128.
[2]	"Yeast checkpoint genes in DNA damage processing: implications for repair and arrest." Lydall D., Weinert T.A. Science 270:1488-1491(1995) [PubMed: 7491494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1." Giannattasio M., Sommariva E., Vercillo R., Lippi-Boncambi F., Liberi G., Foiani M., Plevani P., Muzi-Falconi M. Proc. Natl. Acad. Sci. U.S.A. 99:12997-13002(2002) [PubMed: 12271137] [Abstract] Cited for: FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[6]	"Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint." Majka J., Burgers P.M.J. Proc. Natl. Acad. Sci. U.S.A. 100:2249-2254(2003) [PubMed: 12604797] [Abstract] Cited for: INTERACTION OF THE CHECKPOINT CLAMP COMPLEX WITH THE RFC-RAD24 CHECKPOINT CLAMP LOADER COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX, LACK OF EXONUCLEASE ACTIVITY.
[7]	"Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway." Kondo T., Matsumoto K., Sugimoto K. Mol. Cell. Biol. 19:1136-1143(1999) [PubMed: 9891048] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[8]	"Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1." Giannattasio M., Sabbioneda S., Minuzzo M., Plevani P., Muzi-Falconi M. J. Biol. Chem. 278:22303-22308(2003) [PubMed: 12672803] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Requirement for ATP by the DNA damage checkpoint clamp loader." Majka J., Chung B.Y., Burgers P.M.J. J. Biol. Chem. 279:20921-20926(2004) [PubMed: 15014082] [Abstract] Cited for: INTERACTION OF THE CHECKPOINT CLAMP COMPLEX WITH THE RFC-RAD24 CHECKPOINT CLAMP LOADER COMPLEX.
[11]	"Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA damage checkpoint." Majka J., Burgers P.M.J. DNA Repair 4:1189-1194(2005) [PubMed: 16137930] [Abstract] Cited for: IDENTIFICATION IN THE CHECKPOINT CLAMP COMPLEX, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[12]	"The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae." Sabbioneda S., Minesinger B.K., Giannattasio M., Plevani P., Muzi-Falconi M., Jinks-Robertson S. J. Biol. Chem. 280:38657-38665(2005) [PubMed: 16169844] [Abstract] Cited for: INTERACTION WITH REV7, FUNCTION OF THE CHECKPOINT CLAMP COMPLEX.
[13]	"Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3." Cardone J.M., Revers L.F., Machado R.M., Bonatto D., Brendel M., Henriques J.A.P. DNA Repair 5:163-171(2006) [PubMed: 16202664] [Abstract] Cited for: INTERACTION WITH DDC1 AND MEC3.
[14]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-383, MASS SPECTROMETRY.
[15]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-357, MASS SPECTROMETRY.
[16]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5 AND SER-357, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37460 Genomic DNA. Translation: AAA80545.1. U30796 Genomic DNA. Translation: AAA93250.1. Z75276 Genomic DNA. Translation: CAA99699.1. BK006948 Genomic DNA. Translation: DAA11127.1.
PIR	S59670.
RefSeq	NP_015013.1. NM_001183788.1.
3D structure databases
ProteinModelPortal	P48581.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1546N.
IntAct	P48581. 9 interactions.
MINT	MINT-408918.
STRING	P48581.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR368W; YOR368W; YOR368W.
GeneID	854550.
KEGG	sce:YOR368W.
NMPDR	fig|4932.3.peg.6132.
Organism-specific databases
CYGD	YOR368w.
SGD	S000005895. RAD17.
Phylogenomic databases
eggNOG	fuNOG04822.
HOGENOM	HBG202934.
OMA	EIGCKEC.
OrthoDB	EOG4NW2VQ.
Gene expression databases
ArrayExpress	P48581.
Genevestigator	P48581.
GermOnline	YOR368W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016587. Cell_cycle_RAD17. IPR003021. Rad1_Rec1. [Graphical view]
KO	K02830.
PANTHER	PTHR10870. Rad1_Rec1. 1 hit.
Pfam	PF02144. Rad1. 1 hit. [Graphical view]
PIRSF	PIRSF011769. Cell_cycle_RAD17. 1 hit.
PRINTS	PR01245. RAD1REC1.
ProtoNet	Search...
Other
NextBio	976967.

Entry information

Entry name

RAD17_YEAST

Accession

Primary (citable) accession number: P48581
Secondary accession number(s): D6W361

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program