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P48578

- PP2A3_ARATH

UniProt

P48578 - PP2A3_ARATH

Protein

Serine/threonine-protein phosphatase PP2A-3 catalytic subunit

Gene

PP2A3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611Manganese 1By similarity
    Metal bindingi63 – 631Manganese 1By similarity
    Metal bindingi89 – 891Manganese 1By similarity
    Metal bindingi89 – 891Manganese 2By similarity
    Metal bindingi121 – 1211Manganese 2By similarity
    Active sitei122 – 1221Proton donorBy similarity
    Metal bindingi171 – 1711Manganese 2By similarity
    Metal bindingi245 – 2451Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW

    GO - Biological processi

    1. protein dephosphorylation Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G58500-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP2A-3 catalytic subunit (EC:3.1.3.16)
    Alternative name(s):
    Protein phosphatase 2A isoform 3
    Gene namesi
    Name:PP2A3
    Synonyms:EP7, PP2A4
    Ordered Locus Names:At3g58500
    ORF Names:F14P22.90
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G58500.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. cytosol Source: TAIR
    3. nucleolus Source: TAIR
    4. nucleus Source: TAIR
    5. protein phosphatase type 2A complex Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Serine/threonine-protein phosphatase PP2A-3 catalytic subunitPRO_0000058855Add
    BLAST

    Proteomic databases

    PaxDbiP48578.
    PRIDEiP48578.

    Expressioni

    Gene expression databases

    GenevestigatoriP48578.

    Interactioni

    Subunit structurei

    PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families).By similarity

    Protein-protein interaction databases

    BioGridi10334. 2 interactions.
    IntActiP48578. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP48578.
    SMRiP48578. Positions 11-313.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172696.
    InParanoidiP48578.
    KOiK04382.
    OMAiDPANDSK.
    PhylomeDBiP48578.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48578-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGANSLPTDA TLDLDEQISQ LMQCKPLSEQ QVRALCEKAK EILMDESNVQ    50
    PVKSPVTICG DIHGQFHDLA ELFRIGGKCP DTNYLFMGDY VDRGYYSVET 100
    VTLLVGLKVR YPQRITILRG NHESRQITQV YGFYDECLRK YGNANVWKIF 150
    TDLFDYFPLT ALVESEIFCL HGGLSPSIET LDNIRNFDRV QEVPHEGPMC 200
    DLLWSDPDDR CGWGISPRGA GYTFGQDISE QFNHTNNLKL IARAHQLVMD 250
    GFNWAHEQKV VTIFSAPNYC YRCGNMASIL EVDDCRNHTF IQFEPAPRRG 300
    EPDVTRRTPD YFL 313
    Length:313
    Mass (Da):35,767
    Last modified:February 1, 1996 - v1
    Checksum:i0636FF10832B1B00
    GO

    Sequence cautioni

    The sequence CAB68188.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08047 mRNA. Translation: AAA64941.1.
    U60136 Genomic DNA. Translation: AAD10855.1.
    AL137082 Genomic DNA. Translation: CAB68188.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE79790.1.
    AY057604 mRNA. Translation: AAL14399.1.
    AY056222 mRNA. Translation: AAL07071.1.
    AY113023 mRNA. Translation: AAM47331.1.
    PIRiS52660.
    RefSeqiNP_567066.1. NM_115712.3.
    UniGeneiAt.25267.

    Genome annotation databases

    EnsemblPlantsiAT3G58500.1; AT3G58500.1; AT3G58500.
    GeneIDi825019.
    KEGGiath:AT3G58500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08047 mRNA. Translation: AAA64941.1 .
    U60136 Genomic DNA. Translation: AAD10855.1 .
    AL137082 Genomic DNA. Translation: CAB68188.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE79790.1 .
    AY057604 mRNA. Translation: AAL14399.1 .
    AY056222 mRNA. Translation: AAL07071.1 .
    AY113023 mRNA. Translation: AAM47331.1 .
    PIRi S52660.
    RefSeqi NP_567066.1. NM_115712.3.
    UniGenei At.25267.

    3D structure databases

    ProteinModelPortali P48578.
    SMRi P48578. Positions 11-313.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 10334. 2 interactions.
    IntActi P48578. 1 interaction.

    Proteomic databases

    PaxDbi P48578.
    PRIDEi P48578.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G58500.1 ; AT3G58500.1 ; AT3G58500 .
    GeneIDi 825019.
    KEGGi ath:AT3G58500.

    Organism-specific databases

    TAIRi AT3G58500.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172696.
    InParanoidi P48578.
    KOi K04382.
    OMAi DPANDSK.
    PhylomeDBi P48578.

    Enzyme and pathway databases

    BioCyci ARA:AT3G58500-MONOMER.

    Gene expression databases

    Genevestigatori P48578.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a fourth isoform of the catalytic subunit of protein phosphatase 2A from Arabidopsis thaliana."
      Casamayor A., Perez-Callejon E., Pujol G., Arino J., Ferrer A.
      Plant Mol. Biol. 26:523-528(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia GL1.
      Tissue: Leaf.
    2. "Molecular cloning and characterization of two phosphatase 2A catalytic subunit genes from Arabidopsis thaliana."
      Perez-Callejon E., Casamayor A., Pujol G., Camps M., Ferrer A., Arino J.
      Gene 209:105-112(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPP2A3_ARATH
    AccessioniPrimary (citable) accession number: P48578
    Secondary accession number(s): Q9M2G6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3