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Protein

Serine/threonine-protein phosphatase PP2A-4 catalytic subunit

Gene

PP2A4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions redundantly with PP2A3, and is involved in establishing auxin gradients, apical-basal axis of polarity and root and shoot apical meristem during embryogenesis. May dephosphorylate PIN1 and regulate its subcellular distribution for polar auxin transport (PubMed:23167545). The holoenzyme composed of PP2AA1, PP2A4 and B'ZETA or B'ETA acts as negative regulator of plant innate immunity by controlling BAK1 phosphorylation state and activation in surface-localized immune receptor complexes (PubMed:25085430).2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Manganese 1By similarity1
Metal bindingi63Manganese 1By similarity1
Metal bindingi89Manganese 1By similarity1
Metal bindingi89Manganese 2By similarity1
Metal bindingi121Manganese 2By similarity1
Active sitei122Proton donorBy similarity1
Metal bindingi171Manganese 2By similarity1
Metal bindingi245Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • protein dephosphorylation Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processPlant defense
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-ATH-198753 ERK/MAPK targets
R-ATH-202670 ERKs are inactivated
R-ATH-69231 Cyclin D associated events in G1
R-ATH-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-ATH-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-4 catalytic subunit (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2A isoform 4
Gene namesi
Name:PP2A41 Publication
Synonyms:EP7, PP2A31 Publication
Ordered Locus Names:At3g58500
ORF Names:F14P22.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT3G58500
TAIRilocus:2076451 AT3G58500

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588551 – 313Serine/threonine-protein phosphatase PP2A-4 catalytic subunitAdd BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei311PhosphotyrosineBy similarity1
Modified residuei313Leucine methyl ester1 Publication1

Post-translational modificationi

Reversibly methyl esterified on Leu-313 by leucine carboxyl methyltransferase 1 (LCMT1) and pectin methylesterase 1 (PME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.1 Publication
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP48578
PRIDEiP48578

Expressioni

Gene expression databases

ExpressionAtlasiP48578 baseline and differential
GenevisibleiP48578 AT

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) (By similarity). Interacts with SIC/RON3 (PubMed:26888284).By similarity1 Publication

Protein-protein interaction databases

BioGridi10334, 2 interactors
IntActiP48578, 1 interactor
STRINGi3702.AT3G58500.1

Structurei

3D structure databases

ProteinModelPortaliP48578
SMRiP48578
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

eggNOGiKOG0371 Eukaryota
COG0639 LUCA
HOGENOMiHOG000172696
KOiK04382
OMAiNKIFCPH
OrthoDBiEOG09360EXD
PhylomeDBiP48578

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

P48578-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGANSLPTDA TLDLDEQISQ LMQCKPLSEQ QVRALCEKAK EILMDESNVQ
60 70 80 90 100
PVKSPVTICG DIHGQFHDLA ELFRIGGKCP DTNYLFMGDY VDRGYYSVET
110 120 130 140 150
VTLLVGLKVR YPQRITILRG NHESRQITQV YGFYDECLRK YGNANVWKIF
160 170 180 190 200
TDLFDYFPLT ALVESEIFCL HGGLSPSIET LDNIRNFDRV QEVPHEGPMC
210 220 230 240 250
DLLWSDPDDR CGWGISPRGA GYTFGQDISE QFNHTNNLKL IARAHQLVMD
260 270 280 290 300
GFNWAHEQKV VTIFSAPNYC YRCGNMASIL EVDDCRNHTF IQFEPAPRRG
310
EPDVTRRTPD YFL
Length:313
Mass (Da):35,767
Last modified:February 1, 1996 - v1
Checksum:i0636FF10832B1B00
GO

Sequence cautioni

The sequence CAB68188 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08047 mRNA Translation: AAA64941.1
U60136 Genomic DNA Translation: AAD10855.1
AL137082 Genomic DNA Translation: CAB68188.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE79790.1
AY057604 mRNA Translation: AAL14399.1
AY056222 mRNA Translation: AAL07071.1
AY113023 mRNA Translation: AAM47331.1
PIRiS52660
RefSeqiNP_567066.1, NM_115712.4
UniGeneiAt.25267

Genome annotation databases

EnsemblPlantsiAT3G58500.1; AT3G58500.1; AT3G58500
GeneIDi825019
GrameneiAT3G58500.1; AT3G58500.1; AT3G58500
KEGGiath:AT3G58500

Similar proteinsi

Entry informationi

Entry nameiPP2A4_ARATH
AccessioniPrimary (citable) accession number: P48578
Secondary accession number(s): Q9M2G6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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