Reviewed, UniProtKB/Swiss-Prot P48572 (LEU3_BUCRP)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:

leuB

Encoded on

Plasmid pRPE

Organism

Buchnera aphidicola subsp. Rhopalosiphum padi

Taxonomic identifier

98793 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	3-isopropylmalate dehydrogenase		PRO_0000083660
Regions
	Nucleotide binding	78 – 91	14	NAD By similarity
	Nucleotide binding	285 – 297	13	NAD By similarity
Sites
	Metal binding	227	1	Magnesium or manganese By similarity
	Metal binding	251	1	Magnesium or manganese By similarity
	Metal binding	255	1	Magnesium or manganese By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Site	145	1	Important for catalysis By similarity
	Site	195	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48572-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 3AEEE6E09F345D63	FASTA	363	40,530
	10 20 30 40 50 60 MKKKFRIAVL PGDGIGPEIM REAYKILNIL EKNFSLPLEI REFSVGGIAI DREGIALPEE 70 80 90 100 110 120 TLRGCENSDA ILFGSVGGKK WDYLPIESRP ERASLLPLRK HFNLFSNLRP AKLYSDLKNL 130 140 150 160 170 180 SPLRSNIVRN GFDILCVREL TGGIYFGKPK GRSKNENGEY SFDTEIYYDF EIKRIAHLAF 190 200 210 220 230 240 ELASSRRCKV CSIDKSNVLA SSILWREIVE EVSKNYPNII LSHLYIDNAC MQIIKNPSQF 250 260 270 280 290 300 DVLLCSNIFG DIISDECAMI TGSIGMLPSA SLNSKKFGLY EPAGGSAPDI EGKNIANPIA 310 320 330 340 350 360 QILSLSMLIR HSMNLNEIAD KIDSSVYRAL KAGYRTLDIS DGKNYIKTNE MGDIIAKFLI NGK

References

[1]	"Discovery and molecular characterization of a plasmid localized in Buchnera sp. bacterial endosymbiont of the aphid Rhopalosiphum padi." Bracho A.M., Martinez-Torres D., Moya A., Latorre A. J. Mol. Evol. 41:67-73(1995) [PubMed: 7608990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X71612 Genomic DNA. Translation: CAA50616.1.
3D structure databases
HSSP	HSSP built from PDB template 1CNZ based on UniProtKB P37412.
ModBase	Search...
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BUCRP

Accession

Primary (citable) accession number: P48572

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 4, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)