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P48570

- HOSC_YEAST

UniProt

P48570 - HOSC_YEAST

Protein

Homocitrate synthase, cytosolic isozyme

Gene

LYS20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

    Pathwayi

    GO - Molecular functioni

    1. homocitrate synthase activity Source: SGD

    GO - Biological processi

    1. lysine biosynthetic process via aminoadipic acid Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YDL182W-MONOMER.
    SABIO-RKP48570.
    UniPathwayiUPA00033; UER00028.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homocitrate synthase, cytosolic isozyme (EC:2.3.3.14)
    Gene namesi
    Name:LYS20
    Ordered Locus Names:YDL182W
    ORF Names:D1298
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL182w.
    SGDiS000002341. LYS20.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Homocitrate synthase, cytosolic isozymePRO_0000140454Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei385 – 3851Phosphoserine1 Publication
    Modified residuei396 – 3961Phosphothreonine2 Publications
    Modified residuei401 – 4011Phosphoserine1 Publication
    Modified residuei410 – 4101Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP48570.
    PeptideAtlasiP48570.
    PRIDEiP48570.

    Expressioni

    Gene expression databases

    GenevestigatoriP48570.

    Interactioni

    Protein-protein interaction databases

    BioGridi31862. 90 interactions.
    IntActiP48570. 4 interactions.
    MINTiMINT-2780078.
    STRINGi4932.YDL182W.

    Structurei

    3D structure databases

    ProteinModelPortaliP48570.
    SMRiP48570. Positions 5-392.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000006101.
    HOGENOMiHOG000046858.
    KOiK01655.
    OMAiPICIHCH.
    OrthoDBiEOG76HQ9T.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR011872. Homocitrate_synth_fun/arc.
    IPR000891. PYR_CT.
    [Graphical view]
    PfamiPF00682. HMGL-like. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
    PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48570-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA    50
    RALDDFGVDY IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK 100
    VAVETGVDGV DVVIGTSKFL RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI 150
    RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV GIADTVGCAN PRQVYELIRT 200
    LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL GIGERNGITP 250
    LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC 300
    AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV 350
    DQLNLNLTDD QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL 400
    SAKKNKKNDS DVPELATIPA AKRTKPSA 428
    Length:428
    Mass (Da):47,099
    Last modified:February 1, 1996 - v1
    Checksum:i8774FF56FA678E5E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83276 Genomic DNA. Translation: CAA58264.1.
    Z67750 Genomic DNA. Translation: CAA91564.1.
    Z74230 Genomic DNA. Translation: CAA98757.1.
    Z74229 Genomic DNA. Translation: CAA98756.1.
    BK006938 Genomic DNA. Translation: DAA11680.1.
    PIRiS58735.
    RefSeqiNP_010099.1. NM_001180242.1.

    Genome annotation databases

    EnsemblFungiiYDL182W; YDL182W; YDL182W.
    GeneIDi851346.
    KEGGisce:YDL182W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83276 Genomic DNA. Translation: CAA58264.1 .
    Z67750 Genomic DNA. Translation: CAA91564.1 .
    Z74230 Genomic DNA. Translation: CAA98757.1 .
    Z74229 Genomic DNA. Translation: CAA98756.1 .
    BK006938 Genomic DNA. Translation: DAA11680.1 .
    PIRi S58735.
    RefSeqi NP_010099.1. NM_001180242.1.

    3D structure databases

    ProteinModelPortali P48570.
    SMRi P48570. Positions 5-392.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31862. 90 interactions.
    IntActi P48570. 4 interactions.
    MINTi MINT-2780078.
    STRINGi 4932.YDL182W.

    Proteomic databases

    MaxQBi P48570.
    PeptideAtlasi P48570.
    PRIDEi P48570.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL182W ; YDL182W ; YDL182W .
    GeneIDi 851346.
    KEGGi sce:YDL182W.

    Organism-specific databases

    CYGDi YDL182w.
    SGDi S000002341. LYS20.

    Phylogenomic databases

    GeneTreei ENSGT00390000006101.
    HOGENOMi HOG000046858.
    KOi K01655.
    OMAi PICIHCH.
    OrthoDBi EOG76HQ9T.

    Enzyme and pathway databases

    UniPathwayi UPA00033 ; UER00028 .
    BioCyci YEAST:YDL182W-MONOMER.
    SABIO-RK P48570.

    Miscellaneous databases

    NextBioi 968429.

    Gene expression databases

    Genevestigatori P48570.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR002034. AIPM/Hcit_synth_CS.
    IPR013785. Aldolase_TIM.
    IPR011872. Homocitrate_synth_fun/arc.
    IPR000891. PYR_CT.
    [Graphical view ]
    Pfami PF00682. HMGL-like. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02146. LysS_fung_arch. 1 hit.
    PROSITEi PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
    PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
    PS50991. PYR_CT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae."
      Verhasselt P., Voet M., Volckaert G.
      Yeast 11:961-966(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Identification of a gene encoding a homocitrate synthase isoenzyme of Saccharomyces cerevisiae."
      Ramos F., Verhasselt P., Feller A., Peeters P., Wach A., Dubois E., Volckaert G.
      Yeast 12:1315-1320(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-396, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-401, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-385; THR-396 AND SER-410, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHOSC_YEAST
    AccessioniPrimary (citable) accession number: P48570
    Secondary accession number(s): D6VRH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 28100 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3