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Protein

Homocitrate synthase, cytosolic isozyme

Gene

LYS20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, cytosolic isozyme (LYS20), Homocitrate synthase, mitochondrial (LYS21)
  2. Homocitrate dehydratase, mitochondrial (ACO2)
  3. Homoaconitase, mitochondrial (LYS4)
  4. Homoisocitrate dehydrogenase, mitochondrial (LYS12)
  5. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • homocitrate synthase activity Source: SGD

GO - Biological processi

  • DNA repair Source: SGD
  • histone displacement Source: SGD
  • lysine biosynthetic process via aminoadipic acid Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YDL182W-MONOMER.
BRENDAi2.3.3.14. 984.
SABIO-RKP48570.
UniPathwayiUPA00033; UER00028.

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate synthase, cytosolic isozyme (EC:2.3.3.14)
Gene namesi
Name:LYS20
Ordered Locus Names:YDL182W
ORF Names:D1298
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL182W.
SGDiS000002341. LYS20.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404541 – 428Homocitrate synthase, cytosolic isozymeAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei385PhosphoserineCombined sources1
Modified residuei396PhosphothreonineCombined sources1
Modified residuei401PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48570.
PRIDEiP48570.

PTM databases

iPTMnetiP48570.

Interactioni

Protein-protein interaction databases

BioGridi31862. 94 interactors.
IntActiP48570. 5 interactors.
MINTiMINT-2780078.

Structurei

3D structure databases

ProteinModelPortaliP48570.
SMRiP48570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 276Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000006101.
HOGENOMiHOG000046858.
InParanoidiP48570.
KOiK01655.
OMAiRDYVIDK.
OrthoDBiEOG092C2OYT.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA
60 70 80 90 100
RALDDFGVDY IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK
110 120 130 140 150
VAVETGVDGV DVVIGTSKFL RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI
160 170 180 190 200
RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV GIADTVGCAN PRQVYELIRT
210 220 230 240 250
LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL GIGERNGITP
260 270 280 290 300
LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC
310 320 330 340 350
AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV
360 370 380 390 400
DQLNLNLTDD QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL
410 420
SAKKNKKNDS DVPELATIPA AKRTKPSA
Length:428
Mass (Da):47,099
Last modified:February 1, 1996 - v1
Checksum:i8774FF56FA678E5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA. Translation: CAA58264.1.
Z67750 Genomic DNA. Translation: CAA91564.1.
Z74230 Genomic DNA. Translation: CAA98757.1.
Z74229 Genomic DNA. Translation: CAA98756.1.
BK006938 Genomic DNA. Translation: DAA11680.1.
PIRiS58735.
RefSeqiNP_010099.1. NM_001180242.1.

Genome annotation databases

EnsemblFungiiYDL182W; YDL182W; YDL182W.
GeneIDi851346.
KEGGisce:YDL182W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA. Translation: CAA58264.1.
Z67750 Genomic DNA. Translation: CAA91564.1.
Z74230 Genomic DNA. Translation: CAA98757.1.
Z74229 Genomic DNA. Translation: CAA98756.1.
BK006938 Genomic DNA. Translation: DAA11680.1.
PIRiS58735.
RefSeqiNP_010099.1. NM_001180242.1.

3D structure databases

ProteinModelPortaliP48570.
SMRiP48570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31862. 94 interactors.
IntActiP48570. 5 interactors.
MINTiMINT-2780078.

PTM databases

iPTMnetiP48570.

Proteomic databases

MaxQBiP48570.
PRIDEiP48570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL182W; YDL182W; YDL182W.
GeneIDi851346.
KEGGisce:YDL182W.

Organism-specific databases

EuPathDBiFungiDB:YDL182W.
SGDiS000002341. LYS20.

Phylogenomic databases

GeneTreeiENSGT00390000006101.
HOGENOMiHOG000046858.
InParanoidiP48570.
KOiK01655.
OMAiRDYVIDK.
OrthoDBiEOG092C2OYT.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00028.
BioCyciYEAST:YDL182W-MONOMER.
BRENDAi2.3.3.14. 984.
SABIO-RKP48570.

Miscellaneous databases

PROiP48570.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOSC_YEAST
AccessioniPrimary (citable) accession number: P48570
Secondary accession number(s): D6VRH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 28100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.