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Protein

Homocitrate synthase, cytosolic isozyme

Gene

LYS20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 28100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, cytosolic isozyme (LYS20), Homocitrate synthase, mitochondrial (LYS21)
  2. Homocitrate dehydratase, mitochondrial (ACO2)
  3. Homoaconitase, mitochondrial (LYS4)
  4. Homoisocitrate dehydrogenase, mitochondrial (LYS12)
  5. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • homocitrate synthase activity Source: SGD

GO - Biological processi

  • DNA repair Source: SGD
  • histone displacement Source: SGD
  • lysine biosynthetic process via aminoadipic acid Source: SGD

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YDL182W-MONOMER
YEAST:YDL182W-MONOMER
BRENDAi2.3.3.14 984
SABIO-RKiP48570
UniPathwayiUPA00033; UER00028

Protein family/group databases

MoonProtiP48570

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate synthase, cytosolic isozyme (EC:2.3.3.14)
Gene namesi
Name:LYS20
Ordered Locus Names:YDL182W
ORF Names:D1298
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL182W
SGDiS000002341 LYS20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001404541 – 428Homocitrate synthase, cytosolic isozymeAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei385PhosphoserineCombined sources1
Modified residuei396PhosphothreonineCombined sources1
Modified residuei401PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48570
PaxDbiP48570
PRIDEiP48570

PTM databases

iPTMnetiP48570

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31862, 134 interactors
IntActiP48570, 43 interactors
MINTiP48570
STRINGi4932.YDL182W

Structurei

3D structure databases

ProteinModelPortaliP48570
SMRiP48570
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 276Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000046858
InParanoidiP48570
KOiK01655
OMAiSNMFAHE
OrthoDBiEOG092C2OYT

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR002034 AIPM/Hcit_synth_CS
IPR013785 Aldolase_TIM
IPR011872 Homocitrate_synth_fun/arc
IPR000891 PYR_CT
PfamiView protein in Pfam
PF00682 HMGL-like, 1 hit
TIGRFAMsiTIGR02146 LysS_fung_arch, 1 hit
PROSITEiView protein in PROSITE
PS00815 AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816 AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

P48570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA
60 70 80 90 100
RALDDFGVDY IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK
110 120 130 140 150
VAVETGVDGV DVVIGTSKFL RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI
160 170 180 190 200
RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV GIADTVGCAN PRQVYELIRT
210 220 230 240 250
LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL GIGERNGITP
260 270 280 290 300
LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC
310 320 330 340 350
AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV
360 370 380 390 400
DQLNLNLTDD QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL
410 420
SAKKNKKNDS DVPELATIPA AKRTKPSA
Length:428
Mass (Da):47,099
Last modified:February 1, 1996 - v1
Checksum:i8774FF56FA678E5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83276 Genomic DNA Translation: CAA58264.1
Z67750 Genomic DNA Translation: CAA91564.1
Z74230 Genomic DNA Translation: CAA98757.1
Z74229 Genomic DNA Translation: CAA98756.1
BK006938 Genomic DNA Translation: DAA11680.1
PIRiS58735
RefSeqiNP_010099.1, NM_001180242.1

Genome annotation databases

EnsemblFungiiYDL182W; YDL182W; YDL182W
GeneIDi851346
KEGGisce:YDL182W

Similar proteinsi

Entry informationi

Entry nameiHOSC_YEAST
AccessioniPrimary (citable) accession number: P48570
Secondary accession number(s): D6VRH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 25, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health