P48567 (PUS4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase 4
EC=5.4.99.25

Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase 4
tRNA-uridine isomerase 4

Gene names

Name:	PUS4
Ordered Locus Names:	YNL292W
ORF Names:	N0480

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs. Ref.4
Catalytic activity	tRNA uridine(55) = tRNA pseudouridine(55). Ref.4
Subcellular location	Nucleus. Mitochondrion.
Miscellaneous	Present with 3990 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the pseudouridine synthase TruB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion Nucleus
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA modification Inferred from direct assay Ref.4. Source: SGD
Cellular component	mitochondrion Inferred from mutant phenotype Ref.4. Source: SGD nucleus Inferred from mutant phenotype Ref.4. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	tRNA pseudouridine synthase 4		PRO_0000121976
Sites
	Active site	75	1	Nucleophile By similarity
Amino acid modifications
	Modified residue	254	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P48567 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 5E9D652F07362A27	FASTA	403	45,273
	10 20 30 40 50 60 MNGIFAIEKP SGITSNQFML KLQHALTKSQ VFSKEIQRAT AERKQQYEKQ TGKKASKRKL 70 80 90 100 110 120 RKVSKVKMGH GGTLDPLASG VLVIGIGAGT KKLANYLSGT VKVYESEALF GVSTTSGDVE 130 140 150 160 170 180 GEILSQNSVK HLNFDDLKTV EEKFVGQLKQ TPPIYAALKM DGKPLHEYAR EGKPLPRAIE 190 200 210 220 230 240 PRQVTIYDLK VFSDSLKRDH DYPLLRPTTE EAVDTVKNLN ANMLNDVLYF SKEYTEKHGL 250 260 270 280 290 300 DSEVAKVEEP FPLSEQEEQE IQKEGDSYRA PKLHFKANVS SGTYIRSLVS DIGKSMRSSC 310 320 330 340 350 360 YMVKLIRLQQ QDWSLEKNNV FQLTDFTERD EKVWSKVLEK VLDEGATVDV IEELKKAEKE 370 380 390 400 IPADVKECIV SSDQPGDEAT AETIETANAE EHSNTLKRKI EQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs." Becker H.F., Motorin Y., Planta R.J., Grosjean H. Nucleic Acids Res. 25:4493-4499(1997) [PubMed: 9358157] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23084 Genomic DNA. Translation: AAC49108.1. Z71568 Genomic DNA. Translation: CAA96210.1. BK006947 Genomic DNA. Translation: DAA10267.1.
PIR	S60410.
RefSeq	NP_014107.1. NM_001183130.1.
3D structure databases
ProteinModelPortal	P48567.
SMR	P48567. Positions 1-358.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4256N.
IntAct	P48567. 1 interaction.
MINT	MINT-524912.
STRING	P48567.
Proteomic databases
PeptideAtlas	P48567.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL292W; YNL292W; YNL292W.
GeneID	855424.
KEGG	sce:YNL292W.
NMPDR	fig|4932.3.peg.5169.
Organism-specific databases
CYGD	YNL292w.
SGD	S000005236. PUS4.
Phylogenomic databases
eggNOG	fuNOG05604.
GeneTree	EFGT00050000005563.
HOGENOM	HBG397258.
OMA	FAIEKPS.
OrthoDB	EOG4V9Z0D.
Gene expression databases
ArrayExpress	P48567.
Genevestigator	P48567.
GermOnline	YNL292W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002501. PsdUridine_synth. IPR020103. PsdUridine_synth_cat_dom. IPR014780. tRNA_psdUridine_synth_TruB. [Graphical view]
KO	K03177.
PANTHER	PTHR13767. PTHR13767. 1 hit.
Pfam	PF01509. TruB_N. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
ProtoNet	Search...
Other
NextBio	979289.

Entry information

Entry name

PUS4_YEAST

Accession

Primary (citable) accession number: P48567
Secondary accession number(s): D6W0Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program