Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA pseudouridine synthase 4

Gene

PUS4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.1 Publication

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75NucleophileBy similarity1

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • mRNA pseudouridine synthesis Source: SGD
  • tRNA modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciYEAST:YNL292W-MONOMER.
BRENDAi5.4.99.25. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase 4 (EC:5.4.99.25)
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name:
Psi55 synthase
tRNA pseudouridylate synthase 4
tRNA-uridine isomerase 4
Gene namesi
Name:PUS4
Ordered Locus Names:YNL292W
ORF Names:N0480
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL292W.
SGDiS000005236. PUS4.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001219761 – 403tRNA pseudouridine synthase 4Add BLAST403

Proteomic databases

MaxQBiP48567.
PRIDEiP48567.

PTM databases

iPTMnetiP48567.

Interactioni

Protein-protein interaction databases

BioGridi35545. 33 interactors.
DIPiDIP-4256N.
IntActiP48567. 1 interactor.
MINTiMINT-524912.

Structurei

3D structure databases

ProteinModelPortaliP48567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063611.
HOGENOMiHOG000176412.
InParanoidiP48567.
KOiK03177.
OMAiLNDSPLY.
OrthoDBiEOG092C4LPM.

Family and domain databases

HAMAPiMF_01080. TruB_bact. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 3 hits.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 3 hits.

Sequencei

Sequence statusi: Complete.

P48567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGIFAIEKP SGITSNQFML KLQHALTKSQ VFSKEIQRAT AERKQQYEKQ
60 70 80 90 100
TGKKASKRKL RKVSKVKMGH GGTLDPLASG VLVIGIGAGT KKLANYLSGT
110 120 130 140 150
VKVYESEALF GVSTTSGDVE GEILSQNSVK HLNFDDLKTV EEKFVGQLKQ
160 170 180 190 200
TPPIYAALKM DGKPLHEYAR EGKPLPRAIE PRQVTIYDLK VFSDSLKRDH
210 220 230 240 250
DYPLLRPTTE EAVDTVKNLN ANMLNDVLYF SKEYTEKHGL DSEVAKVEEP
260 270 280 290 300
FPLSEQEEQE IQKEGDSYRA PKLHFKANVS SGTYIRSLVS DIGKSMRSSC
310 320 330 340 350
YMVKLIRLQQ QDWSLEKNNV FQLTDFTERD EKVWSKVLEK VLDEGATVDV
360 370 380 390 400
IEELKKAEKE IPADVKECIV SSDQPGDEAT AETIETANAE EHSNTLKRKI

EQV
Length:403
Mass (Da):45,273
Last modified:February 1, 1996 - v1
Checksum:i5E9D652F07362A27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23084 Genomic DNA. Translation: AAC49108.1.
Z71568 Genomic DNA. Translation: CAA96210.1.
BK006947 Genomic DNA. Translation: DAA10267.1.
PIRiS60410.
RefSeqiNP_014107.1. NM_001183130.1.

Genome annotation databases

EnsemblFungiiYNL292W; YNL292W; YNL292W.
GeneIDi855424.
KEGGisce:YNL292W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23084 Genomic DNA. Translation: AAC49108.1.
Z71568 Genomic DNA. Translation: CAA96210.1.
BK006947 Genomic DNA. Translation: DAA10267.1.
PIRiS60410.
RefSeqiNP_014107.1. NM_001183130.1.

3D structure databases

ProteinModelPortaliP48567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35545. 33 interactors.
DIPiDIP-4256N.
IntActiP48567. 1 interactor.
MINTiMINT-524912.

PTM databases

iPTMnetiP48567.

Proteomic databases

MaxQBiP48567.
PRIDEiP48567.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL292W; YNL292W; YNL292W.
GeneIDi855424.
KEGGisce:YNL292W.

Organism-specific databases

EuPathDBiFungiDB:YNL292W.
SGDiS000005236. PUS4.

Phylogenomic databases

GeneTreeiENSGT00530000063611.
HOGENOMiHOG000176412.
InParanoidiP48567.
KOiK03177.
OMAiLNDSPLY.
OrthoDBiEOG092C4LPM.

Enzyme and pathway databases

BioCyciYEAST:YNL292W-MONOMER.
BRENDAi5.4.99.25. 984.

Miscellaneous databases

PROiP48567.

Family and domain databases

HAMAPiMF_01080. TruB_bact. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR014780. tRNA_psdUridine_synth_TruB.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 3 hits.
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPUS4_YEAST
AccessioniPrimary (citable) accession number: P48567
Secondary accession number(s): D6W0Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3990 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.