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Protein

tRNA pseudouridine synthase 4

Gene

PUS4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (PubMed:9358157). Also catalyzes pseudouridylation of mRNAs with the consensus sequence 5'-GGUUCRA-3' (PubMed:25219674).2 Publications

Miscellaneous

Present with 3990 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).1 Publication
mRNA uridine = mRNA pseudouridine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75NucleophileBy similarity1

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • mRNA pseudouridine synthesis Source: SGD
  • tRNA modification Source: SGD

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciYEAST:YNL292W-MONOMER
BRENDAi5.4.99.25 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase 4 (EC:5.4.99.251 Publication)
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name:
Psi55 synthase
tRNA pseudouridylate synthase 4
tRNA-uridine isomerase 4
Gene namesi
Name:PUS4
Ordered Locus Names:YNL292W
ORF Names:N0480
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL292W
SGDiS000005236 PUS4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001219761 – 403tRNA pseudouridine synthase 4Add BLAST403

Proteomic databases

MaxQBiP48567
PaxDbiP48567
PRIDEiP48567

PTM databases

iPTMnetiP48567

Interactioni

Protein-protein interaction databases

BioGridi35545, 40 interactors
DIPiDIP-4256N
IntActiP48567, 1 interactor
MINTiP48567
STRINGi4932.YNL292W

Structurei

3D structure databases

ProteinModelPortaliP48567
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063611
HOGENOMiHOG000176412
InParanoidiP48567
KOiK03177
OMAiGVFAINK
OrthoDBiEOG092C4LPM

Family and domain databases

HAMAPiMF_01080 TruB_bact, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR002501 PsdUridine_synth_N
IPR014780 tRNA_psdUridine_synth_TruB
PANTHERiPTHR13767 PTHR13767, 1 hit
PfamiView protein in Pfam
PF01509 TruB_N, 1 hit
SUPFAMiSSF55120 SSF55120, 3 hits

Sequencei

Sequence statusi: Complete.

P48567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGIFAIEKP SGITSNQFML KLQHALTKSQ VFSKEIQRAT AERKQQYEKQ
60 70 80 90 100
TGKKASKRKL RKVSKVKMGH GGTLDPLASG VLVIGIGAGT KKLANYLSGT
110 120 130 140 150
VKVYESEALF GVSTTSGDVE GEILSQNSVK HLNFDDLKTV EEKFVGQLKQ
160 170 180 190 200
TPPIYAALKM DGKPLHEYAR EGKPLPRAIE PRQVTIYDLK VFSDSLKRDH
210 220 230 240 250
DYPLLRPTTE EAVDTVKNLN ANMLNDVLYF SKEYTEKHGL DSEVAKVEEP
260 270 280 290 300
FPLSEQEEQE IQKEGDSYRA PKLHFKANVS SGTYIRSLVS DIGKSMRSSC
310 320 330 340 350
YMVKLIRLQQ QDWSLEKNNV FQLTDFTERD EKVWSKVLEK VLDEGATVDV
360 370 380 390 400
IEELKKAEKE IPADVKECIV SSDQPGDEAT AETIETANAE EHSNTLKRKI

EQV
Length:403
Mass (Da):45,273
Last modified:February 1, 1996 - v1
Checksum:i5E9D652F07362A27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23084 Genomic DNA Translation: AAC49108.1
Z71568 Genomic DNA Translation: CAA96210.1
BK006947 Genomic DNA Translation: DAA10267.1
PIRiS60410
RefSeqiNP_014107.1, NM_001183130.1

Genome annotation databases

EnsemblFungiiYNL292W; YNL292W; YNL292W
GeneIDi855424
KEGGisce:YNL292W

Similar proteinsi

Entry informationi

Entry nameiPUS4_YEAST
AccessioniPrimary (citable) accession number: P48567
Secondary accession number(s): D6W0Q1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 20, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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