Reviewed, UniProtKB/Swiss-Prot P48567 (PUS4_YEAST)

Names and origin

Protein names

Recommended name:
    tRNA pseudouridine synthase 4
    EC=5.4.99.-
Alternative name(s):
    tRNA pseudouridine 55 synthase
      Short name=Psi55 synthase
    tRNA-uridine isomerase 4
    tRNA pseudouridylate synthase 4

Gene names

Name:	PUS4
Ordered Locus Names:	YNL292W
ORF Names:	N0480

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.
Catalytic activity	tRNA uridine = tRNA pseudouridine.
Subcellular location	Nucleus. Mitochondrion.
Miscellaneous	Present with 3990 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the pseudouridine synthase truB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion Nucleus
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA modification Ref.3 Inferred from direct assay. Source: SGD
Cellular component	mitochondrion Ref.3 Inferred from mutant phenotype. Source: SGD nucleus Ref.3 Inferred from mutant phenotype. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct pseudouridine synthase activity Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TEF1	P02994	1	EBI-14291,EBI-6314

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	tRNA pseudouridine synthase 4		PRO_0000121976
Sites
	Active site	75	1	Nucleophile By similarity
Amino acid modifications
	Modified residue	254	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P48567-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 5E9D652F07362A27	FASTA	403	45,273
	10 20 30 40 50 60 MNGIFAIEKP SGITSNQFML KLQHALTKSQ VFSKEIQRAT AERKQQYEKQ TGKKASKRKL 70 80 90 100 110 120 RKVSKVKMGH GGTLDPLASG VLVIGIGAGT KKLANYLSGT VKVYESEALF GVSTTSGDVE 130 140 150 160 170 180 GEILSQNSVK HLNFDDLKTV EEKFVGQLKQ TPPIYAALKM DGKPLHEYAR EGKPLPRAIE 190 200 210 220 230 240 PRQVTIYDLK VFSDSLKRDH DYPLLRPTTE EAVDTVKNLN ANMLNDVLYF SKEYTEKHGL 250 260 270 280 290 300 DSEVAKVEEP FPLSEQEEQE IQKEGDSYRA PKLHFKANVS SGTYIRSLVS DIGKSMRSSC 310 320 330 340 350 360 YMVKLIRLQQ QDWSLEKNNV FQLTDFTERD EKVWSKVLEK VLDEGATVDV IEELKKAEKE 370 380 390 400 IPADVKECIV SSDQPGDEAT AETIETANAE EHSNTLKRKI EQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs." Becker H.F., Motorin Y., Planta R.J., Grosjean H. Nucleic Acids Res. 25:4493-4499(1997) [PubMed: 9358157] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23084 Genomic DNA. Translation: AAC49108.1. Z71568 Genomic DNA. Translation: CAA96210.1.
PIR	S60410.
RefSeq	NP_014107.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4256N.
IntAct	P48567. 2 interactions.
STRING	P48567.
Proteomic databases
PeptideAtlas	P48567.
PRIDE	P48567.
Genome annotation databases
Ensembl	YNL292W; YNL292W; YNL292W; Saccharomyces cerevisiae. [Genome view]
GeneID	855424.
KEGG	sce:YNL292W.
NMPDR	fig|4932.3.peg.5169.
Organism-specific databases
CYGD	YNL292w.
SGD	S000005236. PUS4.
Phylogenomic databases
HOGENOM	HBG397258.
OMA	FAIEKPS.
OrthoDB	EOG98SJ9C.
Gene expression databases
ArrayExpress	P48567.
Genevestigator	P48567.
GermOnline	YNL292W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR002501. PsdUridine_synth_TruB_N. [Graphical view]
Pfam	PF01509. TruB_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979289.

Entry information

Entry name

PUS4_YEAST

Accession

Primary (citable) accession number: P48567

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)