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P48566

- GYP3_YEAST

UniProt

P48566 - GYP3_YEAST

Protein

GTPase-activating protein GYP3

Gene

MSB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Regulates exocytosis by functioning as a GAP for SEC4. Stimulates specifically the GTPase activity of YPT6. Also required for efficient polarization of the actin patches.2 Publications

    GO - Molecular functioni

    1. Rab GTPase activator activity Source: SGD

    GO - Biological processi

    1. activation of Rab GTPase activity Source: SGD
    2. endocytosis Source: SGD
    3. exocytosis Source: SGD
    4. formin-nucleated actin cable assembly Source: SGD
    5. regulation of GTPase activity Source: SGD
    6. regulation of protein localization Source: SGD

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33282-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase-activating protein GYP3
    Alternative name(s):
    Multicopy suppressor of bud emergence 3
    Protein MSB3
    Gene namesi
    Name:MSB3
    Synonyms:GYP3
    Ordered Locus Names:YNL293W
    ORF Names:N0470
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL293w.
    SGDiS000005237. MSB3.

    Subcellular locationi

    Cytoplasm 1 Publication. Bud 1 Publication. Bud neck 1 Publication
    Note: Localizes to the presumptive bud site, the bud tip and the mother-bud neck.

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. cytosol Source: SGD
    4. fungal-type vacuole membrane Source: SGD
    5. incipient cellular bud site Source: SGD
    6. late endosome Source: SGD
    7. plasma membrane Source: SGD
    8. polarisome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi282 – 2821R → F or K: Reduced GAP activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 633633GTPase-activating protein GYP3PRO_0000208012Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei147 – 1471Phosphoserine1 Publication
    Modified residuei484 – 4841Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP48566.
    PaxDbiP48566.
    PeptideAtlasiP48566.

    Expressioni

    Gene expression databases

    GenevestigatoriP48566.

    Interactioni

    Protein-protein interaction databases

    BioGridi35544. 65 interactions.
    DIPiDIP-2783N.
    IntActiP48566. 3 interactions.
    MINTiMINT-1758444.
    STRINGi4932.YNL293W.

    Structurei

    3D structure databases

    ProteinModelPortaliP48566.
    SMRiP48566. Positions 216-475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini223 – 456234Rab-GAP TBCPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5210.
    GeneTreeiENSGT00730000110571.
    HOGENOMiHOG000248756.
    OMAiTERIIPK.
    OrthoDBiEOG72G1GN.

    Family and domain databases

    InterProiIPR000195. Rab-GTPase-TBC_dom.
    [Graphical view]
    PfamiPF00566. RabGAP-TBC. 1 hit.
    [Graphical view]
    SMARTiSM00164. TBC. 1 hit.
    [Graphical view]
    SUPFAMiSSF47923. SSF47923. 2 hits.
    PROSITEiPS50086. TBC_RABGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNDQQRFSL QNRTVLAHPY KRLGGAFTVK SPSVPNFHDK MHSDHSSSDS    50
    ALVNGSFRAN DHRSVEPSCL GQASPSEHDG NLSVIDLYGD EVESQRAEGE 100
    DDDDNNGDNG NEDLEEVHSD DLDLVPDDDN RQRVELEGAA SATSANSNGI 150
    NNTHFDRYGF KKQNNYISEA EYDKWWVEYS QYCVRRKHKW QLLLEKSGLP 200
    VTDDSPSRFP SKSERLKRYV RKGIPAEWRG NAWWHFARGQ EKLNKNKGVY 250
    SQLLRKMKQI KKQNPNEKQV QDLDIIERDL NRTFPDNIHF QSSLHNKEGP 300
    PIIKSLRRVL VAFSLYNPKI GYCQSMNFLA GLLLLFLDEE RAFWMLVIIT 350
    SRYLPGVHNI NLEGVNIDQG VLMLCVKEYI PEVWSYIKPS IDHHQKNNKT 400
    FSPSNKKVLF NMQKNEFLYR LPPITLCTAS WFMSCFVGVV PIETTLRIWD 450
    CLFYEESHFL FKVSLAVLKL SEHDLSKIKP RNNSLNYSWG SNLNQRGGSM 500
    GQEDSDMEIF QVIQTFPKTL LNPNEIFEKI IFKRRFNLNR LDQDEIDRCR 550
    KFVAAQRLKF KTYGELLGNS TSEADLPIND NTDNKGIHIT SDAVNEALSS 600
    EVYGFKKSLA GVHWNNSIKE KVKQMRKKKD KGD 633
    Length:633
    Mass (Da):72,999
    Last modified:February 1, 1996 - v1
    Checksum:i5F62607B95BBAFB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23084 Genomic DNA. Translation: AAC49106.1.
    Z71569 Genomic DNA. Translation: CAA96211.1.
    BK006947 Genomic DNA. Translation: DAA10266.1.
    PIRiS60408.
    RefSeqiNP_014106.1. NM_001183131.1.

    Genome annotation databases

    EnsemblFungiiYNL293W; YNL293W; YNL293W.
    GeneIDi855423.
    KEGGisce:YNL293W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23084 Genomic DNA. Translation: AAC49106.1 .
    Z71569 Genomic DNA. Translation: CAA96211.1 .
    BK006947 Genomic DNA. Translation: DAA10266.1 .
    PIRi S60408.
    RefSeqi NP_014106.1. NM_001183131.1.

    3D structure databases

    ProteinModelPortali P48566.
    SMRi P48566. Positions 216-475.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35544. 65 interactions.
    DIPi DIP-2783N.
    IntActi P48566. 3 interactions.
    MINTi MINT-1758444.
    STRINGi 4932.YNL293W.

    Proteomic databases

    MaxQBi P48566.
    PaxDbi P48566.
    PeptideAtlasi P48566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL293W ; YNL293W ; YNL293W .
    GeneIDi 855423.
    KEGGi sce:YNL293W.

    Organism-specific databases

    CYGDi YNL293w.
    SGDi S000005237. MSB3.

    Phylogenomic databases

    eggNOGi COG5210.
    GeneTreei ENSGT00730000110571.
    HOGENOMi HOG000248756.
    OMAi TERIIPK.
    OrthoDBi EOG72G1GN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33282-MONOMER.

    Miscellaneous databases

    NextBioi 979286.
    PROi P48566.

    Gene expression databases

    Genevestigatori P48566.

    Family and domain databases

    InterProi IPR000195. Rab-GTPase-TBC_dom.
    [Graphical view ]
    Pfami PF00566. RabGAP-TBC. 1 hit.
    [Graphical view ]
    SMARTi SM00164. TBC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47923. SSF47923. 2 hits.
    PROSITEi PS50086. TBC_RABGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene."
      Maurer K.C.T., Urbanus J.H.M., Planta R.J.
      Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast."
      Bi E., Chiavetta J.B., Chen H., Chen G.-C., Chan C.S.M., Pringle J.R.
      Mol. Biol. Cell 11:773-793(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. "The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization."
      Gao X.D., Albert S., Tcheperegine S.E., Burd C.G., Gallwitz D., Bi E.
      J. Cell Biol. 162:635-646(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ARG-282.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147 AND SER-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGYP3_YEAST
    AccessioniPrimary (citable) accession number: P48566
    Secondary accession number(s): D6W0Q0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 178 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3