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P48566 (GYP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTPase-activating protein GYP3
Alternative name(s):
Multicopy suppressor of bud emergence 3
Protein MSB3
Gene names
Name:MSB3
Synonyms:GYP3
Ordered Locus Names:YNL293W
ORF Names:N0470
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length633 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates exocytosis by functioning as a GAP for SEC4. Stimulates specifically the GTPase activity of YPT6. Also required for efficient polarization of the actin patches. Ref.4 Ref.5

Subcellular location

Cytoplasm. Bud. Bud neck. Note: Localizes to the presumptive bud site, the bud tip and the mother-bud neck. Ref.4

Miscellaneous

Present with 178 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 Rab-GAP TBC domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 633633GTPase-activating protein GYP3
PRO_0000208012

Regions

Domain223 – 456234Rab-GAP TBC

Amino acid modifications

Modified residue1471Phosphoserine Ref.8
Modified residue4841Phosphoserine Ref.8

Experimental info

Mutagenesis2821R → F or K: Reduced GAP activity. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P48566 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 5F62607B95BBAFB9

FASTA63372,999
        10         20         30         40         50         60 
MQNDQQRFSL QNRTVLAHPY KRLGGAFTVK SPSVPNFHDK MHSDHSSSDS ALVNGSFRAN 

        70         80         90        100        110        120 
DHRSVEPSCL GQASPSEHDG NLSVIDLYGD EVESQRAEGE DDDDNNGDNG NEDLEEVHSD 

       130        140        150        160        170        180 
DLDLVPDDDN RQRVELEGAA SATSANSNGI NNTHFDRYGF KKQNNYISEA EYDKWWVEYS 

       190        200        210        220        230        240 
QYCVRRKHKW QLLLEKSGLP VTDDSPSRFP SKSERLKRYV RKGIPAEWRG NAWWHFARGQ 

       250        260        270        280        290        300 
EKLNKNKGVY SQLLRKMKQI KKQNPNEKQV QDLDIIERDL NRTFPDNIHF QSSLHNKEGP 

       310        320        330        340        350        360 
PIIKSLRRVL VAFSLYNPKI GYCQSMNFLA GLLLLFLDEE RAFWMLVIIT SRYLPGVHNI 

       370        380        390        400        410        420 
NLEGVNIDQG VLMLCVKEYI PEVWSYIKPS IDHHQKNNKT FSPSNKKVLF NMQKNEFLYR 

       430        440        450        460        470        480 
LPPITLCTAS WFMSCFVGVV PIETTLRIWD CLFYEESHFL FKVSLAVLKL SEHDLSKIKP 

       490        500        510        520        530        540 
RNNSLNYSWG SNLNQRGGSM GQEDSDMEIF QVIQTFPKTL LNPNEIFEKI IFKRRFNLNR 

       550        560        570        580        590        600 
LDQDEIDRCR KFVAAQRLKF KTYGELLGNS TSEADLPIND NTDNKGIHIT SDAVNEALSS 

       610        620        630 
EVYGFKKSLA GVHWNNSIKE KVKQMRKKKD KGD 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene."
Maurer K.C.T., Urbanus J.H.M., Planta R.J.
Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast."
Bi E., Chiavetta J.B., Chen H., Chen G.-C., Chan C.S.M., Pringle J.R.
Mol. Biol. Cell 11:773-793(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization."
Gao X.D., Albert S., Tcheperegine S.E., Burd C.G., Gallwitz D., Bi E.
J. Cell Biol. 162:635-646(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ARG-282.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147 AND SER-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U23084 Genomic DNA. Translation: AAC49106.1.
Z71569 Genomic DNA. Translation: CAA96211.1.
BK006947 Genomic DNA. Translation: DAA10266.1.
PIRS60408.
RefSeqNP_014106.1. NM_001183131.1.

3D structure databases

ProteinModelPortalP48566.
SMRP48566. Positions 188-492.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35544. 65 interactions.
DIPDIP-2783N.
IntActP48566. 3 interactions.
MINTMINT-1758444.
STRING4932.YNL293W.

Proteomic databases

PaxDbP48566.
PeptideAtlasP48566.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL293W; YNL293W; YNL293W.
GeneID855423.
KEGGsce:YNL293W.

Organism-specific databases

CYGDYNL293w.
SGDS000005237. MSB3.

Phylogenomic databases

eggNOGCOG5210.
GeneTreeENSGT00730000110571.
HOGENOMHOG000248756.
OMATERIIPK.
OrthoDBEOG72G1GN.

Enzyme and pathway databases

BioCycYEAST:G3O-33282-MONOMER.

Gene expression databases

GenevestigatorP48566.

Family and domain databases

InterProIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMSSF47923. SSF47923. 2 hits.
PROSITEPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979286.
PROP48566.

Entry information

Entry nameGYP3_YEAST
AccessionPrimary (citable) accession number: P48566
Secondary accession number(s): D6W0Q0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families