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P48566

- GYP3_YEAST

UniProt

P48566 - GYP3_YEAST

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Protein

GTPase-activating protein GYP3

Gene

MSB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulates exocytosis by functioning as a GAP for SEC4. Stimulates specifically the GTPase activity of YPT6. Also required for efficient polarization of the actin patches.2 Publications

GO - Molecular functioni

  1. Rab GTPase activator activity Source: SGD

GO - Biological processi

  1. activation of Rab GTPase activity Source: SGD
  2. endocytosis Source: SGD
  3. exocytosis Source: SGD
  4. formin-nucleated actin cable assembly Source: SGD
  5. regulation of GTPase activity Source: SGD
  6. regulation of protein localization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-33282-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein GYP3
Alternative name(s):
Multicopy suppressor of bud emergence 3
Protein MSB3
Gene namesi
Name:MSB3
Synonyms:GYP3
Ordered Locus Names:YNL293W
ORF Names:N0470
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL293w.
SGDiS000005237. MSB3.

Subcellular locationi

Cytoplasm 1 Publication. Bud 1 Publication. Bud neck 1 Publication
Note: Localizes to the presumptive bud site, the bud tip and the mother-bud neck.

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. cytosol Source: SGD
  4. fungal-type vacuole membrane Source: SGD
  5. incipient cellular bud site Source: SGD
  6. late endosome Source: SGD
  7. plasma membrane Source: SGD
  8. polarisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi282 – 2821R → F or K: Reduced GAP activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633GTPase-activating protein GYP3PRO_0000208012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei147 – 1471Phosphoserine1 Publication
Modified residuei484 – 4841Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48566.
PaxDbiP48566.
PeptideAtlasiP48566.

Expressioni

Gene expression databases

GenevestigatoriP48566.

Interactioni

Protein-protein interaction databases

BioGridi35544. 65 interactions.
DIPiDIP-2783N.
IntActiP48566. 3 interactions.
MINTiMINT-1758444.
STRINGi4932.YNL293W.

Structurei

3D structure databases

ProteinModelPortaliP48566.
SMRiP48566. Positions 216-475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini223 – 456234Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5210.
GeneTreeiENSGT00760000119202.
HOGENOMiHOG000248756.
InParanoidiP48566.
OMAiTERIIPK.
OrthoDBiEOG72G1GN.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48566-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQNDQQRFSL QNRTVLAHPY KRLGGAFTVK SPSVPNFHDK MHSDHSSSDS
60 70 80 90 100
ALVNGSFRAN DHRSVEPSCL GQASPSEHDG NLSVIDLYGD EVESQRAEGE
110 120 130 140 150
DDDDNNGDNG NEDLEEVHSD DLDLVPDDDN RQRVELEGAA SATSANSNGI
160 170 180 190 200
NNTHFDRYGF KKQNNYISEA EYDKWWVEYS QYCVRRKHKW QLLLEKSGLP
210 220 230 240 250
VTDDSPSRFP SKSERLKRYV RKGIPAEWRG NAWWHFARGQ EKLNKNKGVY
260 270 280 290 300
SQLLRKMKQI KKQNPNEKQV QDLDIIERDL NRTFPDNIHF QSSLHNKEGP
310 320 330 340 350
PIIKSLRRVL VAFSLYNPKI GYCQSMNFLA GLLLLFLDEE RAFWMLVIIT
360 370 380 390 400
SRYLPGVHNI NLEGVNIDQG VLMLCVKEYI PEVWSYIKPS IDHHQKNNKT
410 420 430 440 450
FSPSNKKVLF NMQKNEFLYR LPPITLCTAS WFMSCFVGVV PIETTLRIWD
460 470 480 490 500
CLFYEESHFL FKVSLAVLKL SEHDLSKIKP RNNSLNYSWG SNLNQRGGSM
510 520 530 540 550
GQEDSDMEIF QVIQTFPKTL LNPNEIFEKI IFKRRFNLNR LDQDEIDRCR
560 570 580 590 600
KFVAAQRLKF KTYGELLGNS TSEADLPIND NTDNKGIHIT SDAVNEALSS
610 620 630
EVYGFKKSLA GVHWNNSIKE KVKQMRKKKD KGD
Length:633
Mass (Da):72,999
Last modified:February 1, 1996 - v1
Checksum:i5F62607B95BBAFB9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23084 Genomic DNA. Translation: AAC49106.1.
Z71569 Genomic DNA. Translation: CAA96211.1.
BK006947 Genomic DNA. Translation: DAA10266.1.
PIRiS60408.
RefSeqiNP_014106.1. NM_001183131.1.

Genome annotation databases

EnsemblFungiiYNL293W; YNL293W; YNL293W.
GeneIDi855423.
KEGGisce:YNL293W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23084 Genomic DNA. Translation: AAC49106.1 .
Z71569 Genomic DNA. Translation: CAA96211.1 .
BK006947 Genomic DNA. Translation: DAA10266.1 .
PIRi S60408.
RefSeqi NP_014106.1. NM_001183131.1.

3D structure databases

ProteinModelPortali P48566.
SMRi P48566. Positions 216-475.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35544. 65 interactions.
DIPi DIP-2783N.
IntActi P48566. 3 interactions.
MINTi MINT-1758444.
STRINGi 4932.YNL293W.

Proteomic databases

MaxQBi P48566.
PaxDbi P48566.
PeptideAtlasi P48566.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL293W ; YNL293W ; YNL293W .
GeneIDi 855423.
KEGGi sce:YNL293W.

Organism-specific databases

CYGDi YNL293w.
SGDi S000005237. MSB3.

Phylogenomic databases

eggNOGi COG5210.
GeneTreei ENSGT00760000119202.
HOGENOMi HOG000248756.
InParanoidi P48566.
OMAi TERIIPK.
OrthoDBi EOG72G1GN.

Enzyme and pathway databases

BioCyci YEAST:G3O-33282-MONOMER.

Miscellaneous databases

NextBioi 979286.
PROi P48566.

Gene expression databases

Genevestigatori P48566.

Family and domain databases

InterProi IPR000195. Rab-GTPase-TBC_dom.
[Graphical view ]
Pfami PF00566. RabGAP-TBC. 1 hit.
[Graphical view ]
SMARTi SM00164. TBC. 1 hit.
[Graphical view ]
SUPFAMi SSF47923. SSF47923. 2 hits.
PROSITEi PS50086. TBC_RABGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene."
    Maurer K.C.T., Urbanus J.H.M., Planta R.J.
    Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast."
    Bi E., Chiavetta J.B., Chen H., Chen G.-C., Chan C.S.M., Pringle J.R.
    Mol. Biol. Cell 11:773-793(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization."
    Gao X.D., Albert S., Tcheperegine S.E., Burd C.G., Gallwitz D., Bi E.
    J. Cell Biol. 162:635-646(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ARG-282.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147 AND SER-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGYP3_YEAST
AccessioniPrimary (citable) accession number: P48566
Secondary accession number(s): D6W0Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 178 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3