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Protein

GTPase-activating protein GYP3

Gene

MSB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates exocytosis by functioning as a GAP for SEC4. Stimulates specifically the GTPase activity of YPT6. Also required for efficient polarization of the actin patches.2 Publications

Miscellaneous

Present with 178 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: SGD

GO - Biological processi

  • activation of GTPase activity Source: SGD
  • endocytosis Source: SGD
  • exocytosis Source: SGD
  • formin-nucleated actin cable assembly Source: SGD
  • regulation of GTPase activity Source: SGD
  • regulation of protein localization Source: SGD

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-33282-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation
R-SCE-6811440 Retrograde transport at the Trans-Golgi-Network
R-SCE-8854214 TBC/RABGAPs

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein GYP3
Alternative name(s):
Multicopy suppressor of bud emergence 3
Protein MSB3
Gene namesi
Name:MSB3
Synonyms:GYP3
Ordered Locus Names:YNL293W
ORF Names:N0470
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL293W
SGDiS000005237 MSB3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi282R → F or K: Reduced GAP activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080121 – 633GTPase-activating protein GYP3Add BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147PhosphoserineCombined sources1
Modified residuei484PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48566
PaxDbiP48566
PRIDEiP48566

PTM databases

CarbonylDBiP48566
iPTMnetiP48566

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA3P094352EBI-28312,EBI-8611

Protein-protein interaction databases

BioGridi35544, 111 interactors
DIPiDIP-2783N
IntActiP48566, 13 interactors
MINTiP48566
STRINGi4932.YNL293W

Structurei

3D structure databases

ProteinModelPortaliP48566
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini223 – 456Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST234

Phylogenomic databases

GeneTreeiENSGT00910000144262
HOGENOMiHOG000248756
InParanoidiP48566
KOiK19953
OMAiAHYQPQI
OrthoDBiEOG092C0Q84

Family and domain databases

InterProiView protein in InterPro
IPR000195 Rab-GTPase-TBC_dom
IPR035969 Rab-GTPase_TBC_sf
PfamiView protein in Pfam
PF00566 RabGAP-TBC, 2 hits
SMARTiView protein in SMART
SM00164 TBC, 1 hit
SUPFAMiSSF47923 SSF47923, 2 hits
PROSITEiView protein in PROSITE
PS50086 TBC_RABGAP, 1 hit

Sequencei

Sequence statusi: Complete.

P48566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNDQQRFSL QNRTVLAHPY KRLGGAFTVK SPSVPNFHDK MHSDHSSSDS
60 70 80 90 100
ALVNGSFRAN DHRSVEPSCL GQASPSEHDG NLSVIDLYGD EVESQRAEGE
110 120 130 140 150
DDDDNNGDNG NEDLEEVHSD DLDLVPDDDN RQRVELEGAA SATSANSNGI
160 170 180 190 200
NNTHFDRYGF KKQNNYISEA EYDKWWVEYS QYCVRRKHKW QLLLEKSGLP
210 220 230 240 250
VTDDSPSRFP SKSERLKRYV RKGIPAEWRG NAWWHFARGQ EKLNKNKGVY
260 270 280 290 300
SQLLRKMKQI KKQNPNEKQV QDLDIIERDL NRTFPDNIHF QSSLHNKEGP
310 320 330 340 350
PIIKSLRRVL VAFSLYNPKI GYCQSMNFLA GLLLLFLDEE RAFWMLVIIT
360 370 380 390 400
SRYLPGVHNI NLEGVNIDQG VLMLCVKEYI PEVWSYIKPS IDHHQKNNKT
410 420 430 440 450
FSPSNKKVLF NMQKNEFLYR LPPITLCTAS WFMSCFVGVV PIETTLRIWD
460 470 480 490 500
CLFYEESHFL FKVSLAVLKL SEHDLSKIKP RNNSLNYSWG SNLNQRGGSM
510 520 530 540 550
GQEDSDMEIF QVIQTFPKTL LNPNEIFEKI IFKRRFNLNR LDQDEIDRCR
560 570 580 590 600
KFVAAQRLKF KTYGELLGNS TSEADLPIND NTDNKGIHIT SDAVNEALSS
610 620 630
EVYGFKKSLA GVHWNNSIKE KVKQMRKKKD KGD
Length:633
Mass (Da):72,999
Last modified:February 1, 1996 - v1
Checksum:i5F62607B95BBAFB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23084 Genomic DNA Translation: AAC49106.1
Z71569 Genomic DNA Translation: CAA96211.1
BK006947 Genomic DNA Translation: DAA10266.1
PIRiS60408
RefSeqiNP_014106.1, NM_001183131.1

Genome annotation databases

EnsemblFungiiYNL293W; YNL293W; YNL293W
GeneIDi855423
KEGGisce:YNL293W

Similar proteinsi

Entry informationi

Entry nameiGYP3_YEAST
AccessioniPrimary (citable) accession number: P48566
Secondary accession number(s): D6W0Q0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health