Reviewed, UniProtKB/Swiss-Prot P48566 (GYP3_YEAST)

Names and origin

Protein names

Recommended name:
    GTPase-activating protein GYP3
Alternative name(s):
    Protein MSB3
    Multicopy suppressor of bud emergence 3

Gene names

Name:	MSB3
Synonyms:	GYP3
Ordered Locus Names:	YNL293W
ORF Names:	N0470

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulates exocytosis by functioning as a GAP for SEC4. Stimulates specifically the GTPase activity of YPT6. Also required for efficient polarization of the actin patches. Ref.3 Ref.4
Subcellular location	Cytoplasm. Bud. Bud neck. Note: Localizes to the presumptive bud site, the bud tip and the mother-bud neck. Ref.3
Miscellaneous	Present with 178 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 Rab-GAP TBC domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin filament organization Inferred from genetic interaction. Source: SGD exocytosis Traceable author statement. Source: SGD regulation of Rab GTPase activity Inferred from electronic annotation. Source: InterPro
Cellular component	cellular bud neck Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud tip Ref.3 Inferred from direct assay. Source: SGD incipient cellular bud site Ref.3 Inferred from direct assay. Source: SGD polarisome Inferred from physical interaction. Source: SGD
Molecular function	Rab GTPase activator activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	GTPase-activating protein GYP3		PRO_0000208012
Regions
	Domain	223 – 456	234	Rab-GAP TBC
Amino acid modifications
	Modified residue	570	1	Phosphoserine Ref.6
	Modified residue	572	1	Phosphoserine Ref.6
Experimental info
	Mutagenesis	282	1	R → F or K: Reduced GAP activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P48566-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 5F62607B95BBAFB9	FASTA	633	72,999
	10 20 30 40 50 60 MQNDQQRFSL QNRTVLAHPY KRLGGAFTVK SPSVPNFHDK MHSDHSSSDS ALVNGSFRAN 70 80 90 100 110 120 DHRSVEPSCL GQASPSEHDG NLSVIDLYGD EVESQRAEGE DDDDNNGDNG NEDLEEVHSD 130 140 150 160 170 180 DLDLVPDDDN RQRVELEGAA SATSANSNGI NNTHFDRYGF KKQNNYISEA EYDKWWVEYS 190 200 210 220 230 240 QYCVRRKHKW QLLLEKSGLP VTDDSPSRFP SKSERLKRYV RKGIPAEWRG NAWWHFARGQ 250 260 270 280 290 300 EKLNKNKGVY SQLLRKMKQI KKQNPNEKQV QDLDIIERDL NRTFPDNIHF QSSLHNKEGP 310 320 330 340 350 360 PIIKSLRRVL VAFSLYNPKI GYCQSMNFLA GLLLLFLDEE RAFWMLVIIT SRYLPGVHNI 370 380 390 400 410 420 NLEGVNIDQG VLMLCVKEYI PEVWSYIKPS IDHHQKNNKT FSPSNKKVLF NMQKNEFLYR 430 440 450 460 470 480 LPPITLCTAS WFMSCFVGVV PIETTLRIWD CLFYEESHFL FKVSLAVLKL SEHDLSKIKP 490 500 510 520 530 540 RNNSLNYSWG SNLNQRGGSM GQEDSDMEIF QVIQTFPKTL LNPNEIFEKI IFKRRFNLNR 550 560 570 580 590 600 LDQDEIDRCR KFVAAQRLKF KTYGELLGNS TSEADLPIND NTDNKGIHIT SDAVNEALSS 610 620 630 EVYGFKKSLA GVHWNNSIKE KVKQMRKKKD KGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast." Bi E., Chiavetta J.B., Chen H., Chen G.-C., Chan C.S.M., Pringle J.R. Mol. Biol. Cell 11:773-793(2000) [PubMed: 10679030] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization." Gao X.D., Albert S., Tcheperegine S.E., Burd C.G., Gallwitz D., Bi E. J. Cell Biol. 162:635-646(2003) [PubMed: 12913108] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ARG-282.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-570 AND SER-572, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23084 Genomic DNA. Translation: AAC49106.1. Z71569 Genomic DNA. Translation: CAA96211.1.
PIR	S60408.
RefSeq	NP_014106.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2783N.
IntAct	P48566. 1 interaction.
STRING	P48566.
Proteomic databases
PeptideAtlas	P48566.
Genome annotation databases
Ensembl	YNL293W; YNL293W; YNL293W; Saccharomyces cerevisiae. [Genome view]
GeneID	855423.
KEGG	sce:YNL293W.
NMPDR	fig|4932.3.peg.5168.
Organism-specific databases
CYGD	YNL293w.
SGD	S000005237. MSB3.
Phylogenomic databases
HOGENOM	P48566.
OMA	QGVLMLC
OrthoDB	EOG9DV73T
Gene expression databases
ArrayExpress	P48566.
Genevestigator	P48566.
GermOnline	YNL293W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000195. RabGAP_TBC. [Graphical view]
Pfam	PF00566. TBC. 1 hit. [Graphical view]
SMART	SM00164. TBC. 1 hit. [Graphical view]
PROSITE	PS50086. TBC_RABGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979286.

Entry information

Entry name

GYP3_YEAST

Accession

Primary (citable) accession number: P48566

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)