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Protein

GTPase-activating protein GYP3

Gene

MSB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates exocytosis by functioning as a GAP for SEC4. Stimulates specifically the GTPase activity of YPT6. Also required for efficient polarization of the actin patches.2 Publications

GO - Molecular functioni

  • GTPase activator activity Source: SGD
  • Rab GTPase binding Source: GO_Central

GO - Biological processi

  • activation of GTPase activity Source: SGD
  • endocytosis Source: SGD
  • exocytosis Source: SGD
  • formin-nucleated actin cable assembly Source: SGD
  • intracellular protein transport Source: GO_Central
  • regulation of GTPase activity Source: SGD
  • regulation of protein localization Source: SGD
  • regulation of vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-33282-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein GYP3
Alternative name(s):
Multicopy suppressor of bud emergence 3
Protein MSB3
Gene namesi
Name:MSB3
Synonyms:GYP3
Ordered Locus Names:YNL293W
ORF Names:N0470
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL293W.
SGDiS000005237. MSB3.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytosol Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • incipient cellular bud site Source: SGD
  • late endosome Source: SGD
  • plasma membrane Source: SGD
  • polarisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi282R → F or K: Reduced GAP activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080121 – 633GTPase-activating protein GYP3Add BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147PhosphoserineCombined sources1
Modified residuei484PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48566.
PRIDEiP48566.

PTM databases

iPTMnetiP48566.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35544. 68 interactors.
DIPiDIP-2783N.
IntActiP48566. 3 interactors.
MINTiMINT-1758444.

Structurei

3D structure databases

ProteinModelPortaliP48566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini223 – 456Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000133698.
HOGENOMiHOG000248756.
InParanoidiP48566.
KOiK19953.
OMAiTERIIPK.
OrthoDBiEOG092C0Q84.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 2 hits.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNDQQRFSL QNRTVLAHPY KRLGGAFTVK SPSVPNFHDK MHSDHSSSDS
60 70 80 90 100
ALVNGSFRAN DHRSVEPSCL GQASPSEHDG NLSVIDLYGD EVESQRAEGE
110 120 130 140 150
DDDDNNGDNG NEDLEEVHSD DLDLVPDDDN RQRVELEGAA SATSANSNGI
160 170 180 190 200
NNTHFDRYGF KKQNNYISEA EYDKWWVEYS QYCVRRKHKW QLLLEKSGLP
210 220 230 240 250
VTDDSPSRFP SKSERLKRYV RKGIPAEWRG NAWWHFARGQ EKLNKNKGVY
260 270 280 290 300
SQLLRKMKQI KKQNPNEKQV QDLDIIERDL NRTFPDNIHF QSSLHNKEGP
310 320 330 340 350
PIIKSLRRVL VAFSLYNPKI GYCQSMNFLA GLLLLFLDEE RAFWMLVIIT
360 370 380 390 400
SRYLPGVHNI NLEGVNIDQG VLMLCVKEYI PEVWSYIKPS IDHHQKNNKT
410 420 430 440 450
FSPSNKKVLF NMQKNEFLYR LPPITLCTAS WFMSCFVGVV PIETTLRIWD
460 470 480 490 500
CLFYEESHFL FKVSLAVLKL SEHDLSKIKP RNNSLNYSWG SNLNQRGGSM
510 520 530 540 550
GQEDSDMEIF QVIQTFPKTL LNPNEIFEKI IFKRRFNLNR LDQDEIDRCR
560 570 580 590 600
KFVAAQRLKF KTYGELLGNS TSEADLPIND NTDNKGIHIT SDAVNEALSS
610 620 630
EVYGFKKSLA GVHWNNSIKE KVKQMRKKKD KGD
Length:633
Mass (Da):72,999
Last modified:February 1, 1996 - v1
Checksum:i5F62607B95BBAFB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23084 Genomic DNA. Translation: AAC49106.1.
Z71569 Genomic DNA. Translation: CAA96211.1.
BK006947 Genomic DNA. Translation: DAA10266.1.
PIRiS60408.
RefSeqiNP_014106.1. NM_001183131.1.

Genome annotation databases

EnsemblFungiiYNL293W; YNL293W; YNL293W.
GeneIDi855423.
KEGGisce:YNL293W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23084 Genomic DNA. Translation: AAC49106.1.
Z71569 Genomic DNA. Translation: CAA96211.1.
BK006947 Genomic DNA. Translation: DAA10266.1.
PIRiS60408.
RefSeqiNP_014106.1. NM_001183131.1.

3D structure databases

ProteinModelPortaliP48566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35544. 68 interactors.
DIPiDIP-2783N.
IntActiP48566. 3 interactors.
MINTiMINT-1758444.

PTM databases

iPTMnetiP48566.

Proteomic databases

MaxQBiP48566.
PRIDEiP48566.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL293W; YNL293W; YNL293W.
GeneIDi855423.
KEGGisce:YNL293W.

Organism-specific databases

EuPathDBiFungiDB:YNL293W.
SGDiS000005237. MSB3.

Phylogenomic databases

GeneTreeiENSGT00860000133698.
HOGENOMiHOG000248756.
InParanoidiP48566.
KOiK19953.
OMAiTERIIPK.
OrthoDBiEOG092C0Q84.

Enzyme and pathway databases

BioCyciYEAST:G3O-33282-MONOMER.

Miscellaneous databases

PROiP48566.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 2 hits.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGYP3_YEAST
AccessioniPrimary (citable) accession number: P48566
Secondary accession number(s): D6W0Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 178 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.