P48565 (PALH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
pH-response regulator protein palH/RIM21

Alternative name(s):
Regulator of IME2 protein 21

Gene names

Name:	RIM21
Synonyms:	PAL2
Ordered Locus Names:	YNL294C
ORF Names:	N0466

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the proteolytic cleavage of the transcription factor RIM101 in response to alkaline ambient pH By similarity. Required for growth at alkaline pH. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the palH/RIM21 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ascospore formation Inferred from mutant phenotype Ref.4. Source: SGD fungal-type cell wall biogenesis Inferred from genetic interaction. Source: SGD invasive growth in response to glucose limitation Inferred from mutant phenotype Ref.4. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	pH-response regulator protein palH/RIM21		PRO_0000058205
Regions
	Topological domain	1 – 94	94	Extracellular Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Topological domain	116 – 127	12	Cytoplasmic Potential
	Transmembrane	128 – 148	21	Helical; Potential
	Topological domain	149 – 178	30	Extracellular Potential
	Transmembrane	179 – 199	21	Helical; Potential
	Topological domain	200 – 209	10	Cytoplasmic Potential
	Transmembrane	210 – 230	21	Helical; Potential
	Topological domain	231 – 247	17	Extracellular Potential
	Transmembrane	248 – 268	21	Helical; Potential
	Topological domain	269 – 280	12	Cytoplasmic Potential
	Transmembrane	281 – 301	21	Helical; Potential
	Topological domain	302 – 307	6	Extracellular Potential
	Transmembrane	308 – 328	21	Helical; Potential
	Topological domain	329 – 533	205	Cytoplasmic Potential
Amino acid modifications
	Modified residue	409	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P48565 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 9492A18512F399CC	FASTA	533	61,532
	10 20 30 40 50 60 MNLWRHSPEE LAAYNSCHPM KLGSGVLIQL PLYDNSAVYA EDITFRSFCC ERVPVYVSTV 70 80 90 100 110 120 LRNSSPYRYL DEVINDWQKF IQVSDYVGGS AEYAIYAVIL SITSNFVITV FLTVICCINI 130 140 150 160 170 180 SGRAYKRILQ LLRIASLLAS LNLTIFITKV LRRLEKEHNV YGVVRAHSIM HIFSDDMTFV 190 200 210 220 230 240 VLDFLATLMF QFCQVGIVIR LFQRAQEKRI IFFIGVILTI TANILWVIPP FANHTTKHRN 250 260 270 280 290 300 DWQILRPFVY LFRIAIATSY ASIVIYHIWQ KKKLWFKFNQ MGLLTLLTIL VVLLLPGFFL 310 320 330 340 350 360 ADVSNLWISE LGEVFNTTCY VTSTVITWEW LDRLNVLERK EEAQSILGRP IFEEEQQDYR 370 380 390 400 410 420 FAKYALRVQN ALTRRESQDA STDRHDTSSN SEVCDLQTIS RYDPEDQISV GRSIDRMHFN 430 440 450 460 470 480 DRGTYKDVAL KKLGYARDKI LYFTDQIVQK SVGHNNSSSS KNEKTKQRKA MVRKRLGLDK 490 500 510 520 530 PGIYIYSTKD VVFNSDEDDD ENAEDEDDDE YEVGSEGNNN SSATFTSDHI GHI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Ambient pH signalling in ascomycetous yeasts involves homologues of the Aspergillus nidulans genes palF and palH." Treton B., Blanchin-Roland S., Lambert M., Lepingle A., Gaillardin C. Mol. Gen. Genet. 263:505-513(2000) [PubMed: 10821185] [Abstract] Cited for: FUNCTION.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23084 Genomic DNA. Translation: AAC49105.1. Z71570 Genomic DNA. Translation: CAA96212.1. BK006947 Genomic DNA. Translation: DAA10265.1.
PIR	S63270.
RefSeq	NP_014105.1. NM_001183132.1.
3D structure databases
ProteinModelPortal	P48565.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-2784389.
STRING	P48565.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL294C; YNL294C; YNL294C.
GeneID	855422.
KEGG	sce:YNL294C.
NMPDR	fig|4932.3.peg.5167.
Organism-specific databases
CYGD	YNL294c.
SGD	S000005238. RIM21.
Phylogenomic databases
eggNOG	fuNOG08240.
GeneTree	EFGT00050000003323.
HOGENOM	HBG202740.
OMA	QSILGRP.
OrthoDB	EOG4PG98P.
Gene expression databases
ArrayExpress	P48565.
Genevestigator	P48565.
GermOnline	YNL294C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014844. PalH. [Graphical view]
Pfam	PF08733. PalH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979283.

Entry information

Entry name

PALH_YEAST

Accession

Primary (citable) accession number: P48565
Secondary accession number(s): D6W0P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program