P48563 (MON2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein MON2

Gene names

Name:	MON2
Synonyms:	YSL2
Ordered Locus Names:	YNL297C
ORF Names:	N0453

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1636 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for traffic between late Golgi and early endosomes. Required for endocytosis and maintenance of vacuolar structure. Ref.4 Ref.7 Ref.9 Ref.11
Subunit structure	Homodimer. Interacts with DOP1, ARL1 and NEO1. Ref.4 Ref.8 Ref.9 Ref.11
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein. Note: Late Golgi. Ref.4 Ref.9 Ref.11
Miscellaneous	Present with 2410 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the MON2 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to endosome transport Inferred from mutant phenotype Ref.11. Source: HGNC endocytosis Inferred from mutant phenotype Ref.4. Source: SGD protein targeting to vacuole Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement Ref.4. Source: SGD endosome Inferred from direct assay Ref.4. Source: SGD extrinsic to membrane Inferred from direct assay Ref.4. Source: SGD trans-Golgi network Inferred from direct assay Ref.11. Source: SGD
Molecular function	guanyl-nucleotide exchange factor activity Inferred from genetic interaction. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DOP1	Q03921	4	EBI-28333,EBI-34442

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1636	1636	Protein MON2		PRO_0000203372
Regions
	Region	60 – 410	351	Golgi membrane targeting and interaction with ARL1
	Region	1501 – 1636	136	Required for function in membrane trafficking
Amino acid modifications
	Modified residue	564	1	Phosphoserine Ref.5 Ref.10 Ref.13
	Modified residue	565	1	Phosphothreonine Ref.13
	Modified residue	567	1	Phosphoserine Ref.5 Ref.13
	Modified residue	569	1	Phosphothreonine Ref.13
	Modified residue	571	1	Phosphoserine Ref.12 Ref.13

Sequences

	Sequence		Length	Mass (Da)
	P48563 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F0B3D8E6B0D994D6	FASTA	1,636	186,836
	10 20 30 40 50 60 MAMNTGGFDS MQRQLEAELR SLSSESKRRN STIRHASDKS IEILKRVHSF EELERHPDFA 70 80 90 100 110 120 LPFVLACQSR NAKMTTLAMQ CLQGLSTVPS IPRSRLSEIL DAFIEATHLA MEIQLKVLQV 130 140 150 160 170 180 VPIFFKTYGK FIYGPLCKKL LLCCSNLLHV PNKAPVVVGT ASATLQQLID EIFDRLSIES 190 200 210 220 230 240 VVDDKQYEVL ISNSESIKVN VYRYDANKLF DNICSLNEIS SNGAVSDEEM LLDIGDIPID 250 260 270 280 290 300 YGLEILESIL KNSQKNLLEC QDLQYLLRVK AIPLLLRCIS SSRHFSTAVR SCRCLKLLIR 310 320 330 340 350 360 KEYLSILELE LEVILSLLIH GISVESNLSG WQRVLSLELF KDLSQDPEIV NTLYMDYDNY 370 380 390 400 410 420 PDKKHVFKYL LKECIVLLNS PEYITFLAPS KVVEKMDSPL ITTENSTVKT KFMHLLDKSN 430 440 450 460 470 480 APSINITYII SLILTICNHL CEGLNKSALE SSPLEKKIED KEREEGTGND STVVKVYSGL 490 500 510 520 530 540 FSGLFELNKL FLYSTSLETS IFHLVVRAFQ KLAHSAGVLS LKDKLRACMK LFSILITNNV 550 560 570 580 590 600 TSSNQYSFND TSKSAKNQHT RNISTSSVTT SPVESTKNPS RSIADSAQNK EMKRRLHPRN 610 620 630 640 650 660 ISSRQVSLLR ALISLSISLG PIFDSESWRY TFLTWQWITY YIYGPSADFK ESFYSEDIPP 670 680 690 700 710 720 PPILTKSDVT SIESSLTKFF ESTSSYSCST FHLVLTRLIL DSKNTLTLEQ TNLNLNNDIG 730 740 750 760 770 780 YHPLDAKDEI IPCIYNKAFF VNKIGELATY NCKKFLFGKN GKELWSLIST YMIKLISNRE 790 800 810 820 830 840 MDNDSLRLYT VRVFTDIIKK ATNEVGNSDE QDNKVKQFGT LENLVIDSLM ATINSIKQLD 850 860 870 880 890 900 IGKQEIYNGT INVESDILFQ LLLTLKEILN EFGELLMNSW TNIFNIINSP FEWTVEDTDF 910 920 930 940 950 960 SVNEDIDDSS LFEGIVQKHK NMIQVSYDVF KLISDDFLQS LPMSVIKFVI DTLVNFVSQK 970 980 990 1000 1010 1020 RNLNISFSSI SQFWLVGDYL RVRFNPETLN LSDEKRRSLS EKINNQKLIE IITSSSSHDW 1030 1040 1050 1060 1070 1080 ELYNGLWIYL LKNLINCTND DRVEVKNGAV QTFFRIIDSH SVCFPPWDLI FLEVIEPLLT 1090 1100 1110 1120 1130 1140 KEWSTEELEN ETDFINVTLQ GLIKLYPEHF KDFKNNTTCA KEWSMLLDFL KRLLSSTSNN 1150 1160 1170 1180 1190 1200 TKNAVILNYQ TLLKEIITIE DVPSDILKKC CEIFTDYNIT YSDLSTNASS KTEYDCIYEL 1210 1220 1230 1240 1250 1260 ITGFPPLYQL ISKYDAMTDE FVEKVLLLFN SAIKYPLLPE FVQDKTKPSS MQKAILSGLD 1270 1280 1290 1300 1310 1320 IFMTNDSKDT EILILLQLST ISILAFDTRE KITKKLGPKL PKASLNRLPT FEAISYMSCS 1330 1340 1350 1360 1370 1380 NLRNRIAKID QFGISTLKAK HILRILKNLA EIIKRKSLIT GSESDEIPIW VLASNCFCDL 1390 1400 1410 1420 1430 1440 SNKIFKSLQE DAENPLKDNF CDLFINVIVV TLQRINPELD NLTEIDDLNE YSKYREILLE 1450 1460 1470 1480 1490 1500 NRIIDLFNER QLDTFIYAVW DCSFLYEFDE LENALMQDCG TFSELSQKLS SFDFSCIFGS 1510 1520 1530 1540 1550 1560 TTNPRFQTKY KCSLECLQDL VNFMLNTNEK LRKLTAPYLS ARIALALRRY ISDEYLIGRA 1570 1580 1590 1600 1610 1620 PIPKLRKTEL ATLLNGLCVI LRGVLDQNST LGNKQIGVEN LQTLSPLILR TIPVSHKMDG 1630 LQDKVLELSL GFTKLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Yeast Ysl2p, homologous to Sec7 domain guanine nucleotide exchange factors, functions in endocytosis and maintenance of vacuole integrity and interacts with the Arf-Like small GTPase Arl1p." Jochum A., Jackson D., Schwarz H., Pipkorn R., Singer-Krueger B. Mol. Cell. Biol. 22:4914-4928(2002) [PubMed: 12052896] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARL1.
[5]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-564 AND SER-567, MASS SPECTROMETRY. Strain: 2124.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae." Bonangelino C.J., Chavez E.M., Bonifacino J.S. Mol. Biol. Cell 13:2486-2501(2002) [PubMed: 12134085] [Abstract] Cited for: IDENTIFICATION, FUNCTION.
[8]	"Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system." Wicky S., Schwarz H., Singer-Krueger B. Mol. Cell. Biol. 24:7402-7418(2004) [PubMed: 15314152] [Abstract] Cited for: INTERACTION WITH NEO1.
[9]	"Yeast Mon2p is a highly conserved protein that functions in the cytoplasm-to-vacuole transport pathway and is required for Golgi homeostasis." Efe J.A., Plattner F., Hulo N., Kressler D., Emr S.D., Deloche O. J. Cell Sci. 118:4751-4764(2005) [PubMed: 16219684] [Abstract] Cited for: SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH DOP1, FUNCTION.
[10]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-564, MASS SPECTROMETRY. Strain: YAL6B.
[11]	"Mon2, a relative of large Arf exchange factors, recruits Dop1 to the Golgi apparatus." Gillingham A.K., Whyte J.R.C., Panic B., Munro S. J. Biol. Chem. 281:2273-2280(2006) [PubMed: 16301316] [Abstract] Cited for: INTERACTION WITH DOP1, SUBCELLULAR LOCATION, FUNCTION.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-571, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-564; THR-565; SER-567; THR-569 AND SER-571, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23084 Genomic DNA. Translation: AAC49101.1. Z71573 Genomic DNA. Translation: CAA96214.1. BK006947 Genomic DNA. Translation: DAA10263.1.
PIR	S60403.
RefSeq	NP_014102.1. NM_001183135.1.
3D structure databases
ProteinModelPortal	P48563.
ModBase	Search...
Protein-protein interaction databases
IntAct	P48563. 4 interactions.
MINT	MINT-2787218.
STRING	P48563.
Proteomic databases
PeptideAtlas	P48563.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL297C; YNL297C; YNL297C.
GeneID	855420.
KEGG	sce:YNL297C.
NMPDR	fig|4932.3.peg.5164.
Organism-specific databases
CYGD	YNL297c.
SGD	S000005241. MON2.
Phylogenomic databases
eggNOG	fuNOG05606.
GeneTree	EFGT00050000004940.
HOGENOM	HBG202733.
OMA	ECEIFIS.
OrthoDB	EOG466ZV3.
Gene expression databases
ArrayExpress	P48563.
Genevestigator	P48563.
GermOnline	YNL297C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	979277.

Entry information

Entry name

MON2_YEAST

Accession

Primary (citable) accession number: P48563
Secondary accession number(s): D6W0P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program