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Protein

Serine/threonine-protein kinase CLA4

Gene

CLA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in budding and cytokinesis.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594ATPPROSITE-ProRule annotation1
Active sitei693Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi552 – 560ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: SGD
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • negative regulation of gene expression Source: SGD
  • negative regulation of sterol import by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of protein import into nucleus Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of exit from mitosis Source: SGD
  • response to pheromone Source: SGD
  • septin ring organization Source: SGD
  • sterol import Source: SGD
  • vacuole inheritance Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33286-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase CLA4 (EC:2.7.11.1)
Gene namesi
Name:CLA4
Ordered Locus Names:YNL298W
ORF Names:N0450
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL298W.
SGDiS000005242. CLA4.

Subcellular locationi

GO - Cellular componenti

  • cellular bud Source: SGD
  • fungal-type vacuole Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858651 – 842Serine/threonine-protein kinase CLA4Add BLAST842

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineCombined sources1
Modified residuei46PhosphoserineCombined sources1
Modified residuei351PhosphoserineCombined sources1
Modified residuei367PhosphoserineCombined sources1
Modified residuei425PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48562.
PRIDEiP48562.

PTM databases

iPTMnetiP48562.

Interactioni

Subunit structurei

Interacts with CDC42.

Binary interactionsi

WithEntry#Exp.IntActNotes
BEM1P293663EBI-4750,EBI-3508
MYO5Q044393EBI-4750,EBI-11687
NBP2Q121633EBI-4750,EBI-34713

Protein-protein interaction databases

BioGridi35540. 627 interactors.
DIPiDIP-2276N.
IntActiP48562. 42 interactors.
MINTiMINT-582371.

Structurei

3D structure databases

ProteinModelPortaliP48562.
SMRiP48562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 179PHPROSITE-ProRule annotationAdd BLAST119
Domaini184 – 197CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini546 – 825Protein kinasePROSITE-ProRule annotationAdd BLAST280

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 144Poly-Ser9
Compositional biasi372 – 387Poly-GlnAdd BLAST16

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000133957.
HOGENOMiHOG000234202.
InParanoidiP48562.
KOiK19833.
OMAiDYANGRT.
OrthoDBiEOG092C2E30.

Family and domain databases

CDDicd01093. CRIB_PAK_like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR033923. PAK_BD.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00786. PBD. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSAAANKI SDNDFQNIGP APRPPSSNSQ GRTCYNQTQP ITKLMSQLDL
60 70 80 90 100
TSASHLGTST SKKKSGWVSY KDDGILSFIW QKRYLMLHDS YVALYKNDKQ
110 120 130 140 150
NDDAILKIPL TSIISVSRTQ LKQYCFELVR CSDRNSVSSG SSSSLNVSSD
160 170 180 190 200
SNSKKSIYIA TKTESDLHSW LDAIFAKCPL LSGVSSPTNF THKVHVGFDP
210 220 230 240 250
ETGSFVGMPT NWEKLLKHSR ITGEDWNNNS AAVIQVLQFY QEYNGAGNPT
260 270 280 290 300
NTLDKPQSGE TSSSQKSLPN SYNDNKLRNN SVNSKSSSGV SSSMVSQRKT
310 320 330 340 350
SQPPNTKSPV SLGSGSLPPI NTKLPTSQSN IPRHLQNVPN QQYPKMRNGH
360 370 380 390 400
SPTNGQFPRG PMHPNNSQRS LQQQQQQQQQ QKQQHQQYPY HHQGPSPSPS
410 420 430 440 450
PSPSPLNPYR PHHNMINPYS KQPQSPLSSQ STQNQAIPRY AQNSSPTAAH
460 470 480 490 500
FQPQRTAPKP PISAPRAPYP SNQNATSNTH VQPVAPKNDQ STPQTMRQAP
510 520 530 540 550
KRPDADVAQP GGVAKPKKPA RPTMSTAEIM SKLKKVTVNA DPSQCFKVIE
560 570 580 590 600
KAGQGASGSV YLAERTHIPT ESNMIELINN DIDEPHVGDK VAIKQMVLSK
610 620 630 640 650
QPRKELIVNE ILVMKDSRHK NIVNFLEAYL RTDDDLWVVM EFMEGGSLTD
660 670 680 690 700
IIENSPTNDN SHSPLTEPQI AYIVRETCQG LKFLHDKHII HRDIKSDNVL
710 720 730 740 750
LDTRARVKIT DFGFCARLTD KRSKRATMVG TPYWMAPEVV KQREYDEKID
760 770 780 790 800
VWSLGIMTIE MLEGEPPYLN EDPLKALYLI ATNGTPKLKH PESLSLEIKR
810 820 830 840
FLSVCLCVDV RYRASTEELL HHGFFNMACD PKDLTSLLEW KE
Length:842
Mass (Da):93,909
Last modified:February 1, 1996 - v1
Checksum:i3A2AB86414561DC6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti390Y → I in CAA57879 (PubMed:7649470).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82499 Genomic DNA. Translation: CAA57879.1.
U23084 Genomic DNA. Translation: AAC49100.1.
Z71574 Genomic DNA. Translation: CAA96216.1.
BK006947 Genomic DNA. Translation: DAA10262.1.
PIRiS60402.
RefSeqiNP_014101.1. NM_001183136.1.

Genome annotation databases

EnsemblFungiiYNL298W; YNL298W; YNL298W.
GeneIDi855418.
KEGGisce:YNL298W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82499 Genomic DNA. Translation: CAA57879.1.
U23084 Genomic DNA. Translation: AAC49100.1.
Z71574 Genomic DNA. Translation: CAA96216.1.
BK006947 Genomic DNA. Translation: DAA10262.1.
PIRiS60402.
RefSeqiNP_014101.1. NM_001183136.1.

3D structure databases

ProteinModelPortaliP48562.
SMRiP48562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35540. 627 interactors.
DIPiDIP-2276N.
IntActiP48562. 42 interactors.
MINTiMINT-582371.

PTM databases

iPTMnetiP48562.

Proteomic databases

MaxQBiP48562.
PRIDEiP48562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL298W; YNL298W; YNL298W.
GeneIDi855418.
KEGGisce:YNL298W.

Organism-specific databases

EuPathDBiFungiDB:YNL298W.
SGDiS000005242. CLA4.

Phylogenomic databases

GeneTreeiENSGT00860000133957.
HOGENOMiHOG000234202.
InParanoidiP48562.
KOiK19833.
OMAiDYANGRT.
OrthoDBiEOG092C2E30.

Enzyme and pathway databases

BioCyciYEAST:G3O-33286-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Miscellaneous databases

PROiP48562.

Family and domain databases

CDDicd01093. CRIB_PAK_like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR033923. PAK_BD.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00786. PBD. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLA4_YEAST
AccessioniPrimary (citable) accession number: P48562
Secondary accession number(s): D6W0P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 175 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.