Serine/threonine-protein kinase CLA4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P48562 (CLA4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 126. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase CLA4
EC=2.7.11.1

Gene names

Name:	CLA4
Ordered Locus Names:	YNL298W
ORF Names:	N0450

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in budding and cytokinesis.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with CDC42.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 PH domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Rho protein signal transduction Traceable author statement. Source: SGD budding cell apical bud growth Traceable author statement. Source: SGD cytokinesis Traceable author statement. Source: SGD establishment of cell polarity Traceable author statement. Source: SGD negative regulation of gene expression Inferred from mutant phenotype. Source: SGD regulation of exit from mitosis Inferred from mutant phenotype. Source: SGD response to pheromone Inferred from genetic interaction. Source: SGD sterol import Inferred from mutant phenotype. Source: SGD vacuole inheritance Inferred from mutant phenotype. Source: SGD
Cellular component	actin cap Traceable author statement. Source: SGD cellular bud Inferred from direct assay. Source: SGD fungal-type vacuole Inferred from direct assay. Source: SGD nucleus Inferred from mutant phenotype. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 842

842

Serine/threonine-protein kinase CLA4

PRO_0000085865

Regions

Domain

61 – 179

119

Domain

184 – 197

CRIB

Domain

546 – 825

280

Protein kinase

Nucleotide binding

552 – 560

ATP By similarity

Compositional bias

136 – 144

Poly-Ser

Compositional bias

372 – 387

Poly-Gln

Sites

Active site

693

Proton acceptor By similarity

Binding site

594

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.7 Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

186

Phosphoserine Ref.8

Modified residue

351

Phosphoserine Ref.6 Ref.7 Ref.8

Modified residue

367

Phosphoserine Ref.5

Modified residue

370

Phosphoserine Ref.5

Modified residue

425

Phosphoserine Ref.8

Modified residue

445

Phosphoserine Ref.7 Ref.8

Experimental info

Sequence conflict

390

Y → I in CAA57879. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P48562 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 3A2AB86414561DC6
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FASTA

842

93,909

        10         20         30         40         50         60 
MSLSAAANKI SDNDFQNIGP APRPPSSNSQ GRTCYNQTQP ITKLMSQLDL TSASHLGTST 

        70         80         90        100        110        120 
SKKKSGWVSY KDDGILSFIW QKRYLMLHDS YVALYKNDKQ NDDAILKIPL TSIISVSRTQ 

       130        140        150        160        170        180 
LKQYCFELVR CSDRNSVSSG SSSSLNVSSD SNSKKSIYIA TKTESDLHSW LDAIFAKCPL 

       190        200        210        220        230        240 
LSGVSSPTNF THKVHVGFDP ETGSFVGMPT NWEKLLKHSR ITGEDWNNNS AAVIQVLQFY 

       250        260        270        280        290        300 
QEYNGAGNPT NTLDKPQSGE TSSSQKSLPN SYNDNKLRNN SVNSKSSSGV SSSMVSQRKT 

       310        320        330        340        350        360 
SQPPNTKSPV SLGSGSLPPI NTKLPTSQSN IPRHLQNVPN QQYPKMRNGH SPTNGQFPRG 

       370        380        390        400        410        420 
PMHPNNSQRS LQQQQQQQQQ QKQQHQQYPY HHQGPSPSPS PSPSPLNPYR PHHNMINPYS 

       430        440        450        460        470        480 
KQPQSPLSSQ STQNQAIPRY AQNSSPTAAH FQPQRTAPKP PISAPRAPYP SNQNATSNTH 

       490        500        510        520        530        540 
VQPVAPKNDQ STPQTMRQAP KRPDADVAQP GGVAKPKKPA RPTMSTAEIM SKLKKVTVNA 

       550        560        570        580        590        600 
DPSQCFKVIE KAGQGASGSV YLAERTHIPT ESNMIELINN DIDEPHVGDK VAIKQMVLSK 

       610        620        630        640        650        660 
QPRKELIVNE ILVMKDSRHK NIVNFLEAYL RTDDDLWVVM EFMEGGSLTD IIENSPTNDN 

       670        680        690        700        710        720 
SHSPLTEPQI AYIVRETCQG LKFLHDKHII HRDIKSDNVL LDTRARVKIT DFGFCARLTD 

       730        740        750        760        770        780 
KRSKRATMVG TPYWMAPEVV KQREYDEKID VWSLGIMTIE MLEGEPPYLN EDPLKALYLI 

       790        800        810        820        830        840 
ATNGTPKLKH PESLSLEIKR FLSVCLCVDV RYRASTEELL HHGFFNMACD PKDLTSLLEW 


KE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast." Cvrckova F., de Virgilio C., Manser E., Pringle J.R., Nasmyth K. Genes Dev. 9:1817-1830(1995) [PubMed: 7649470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K1107.
[2]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-367 AND SER-370, MASS SPECTROMETRY. Strain: 2124.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-351 AND SER-445, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-27; SER-29; SER-46; SER-186; SER-351; SER-425 AND SER-445, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82499 Genomic DNA. Translation: CAA57879.1. U23084 Genomic DNA. Translation: AAC49100.1. Z71574 Genomic DNA. Translation: CAA96216.1. BK006947 Genomic DNA. Translation: DAA10262.1.
PIR	S60402.
RefSeq	NP_014101.1. NM_001183136.1.
3D structure databases
ProteinModelPortal	P48562.
SMR	P48562. Positions 183-245, 526-839.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2276N.
IntAct	P48562. 39 interactions.
MINT	MINT-582371.
STRING	P48562.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL298W; YNL298W; YNL298W.
GeneID	855418.
KEGG	sce:YNL298W.
NMPDR	fig\|4932.3.peg.5163.
Organism-specific databases
CYGD	YNL298w.
SGD	S000005242. CLA4.
Phylogenomic databases
eggNOG	fuNOG05607.
GeneTree	EFGT00050000000597.
HOGENOM	HBG755340.
OMA	GPINFTH.
OrthoDB	EOG4RFQ1T.
Enzyme and pathway databases
BRENDA	2.7.11.1. 984.
Gene expression databases
ArrayExpress	P48562.
Genevestigator	P48562.
GermOnline	YNL298W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000095. PAK_box_Rho-bd. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K08286.
Pfam	PF00786. PBD. 1 hit. PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00285. PBD. 1 hit. SM00233. PH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50108. CRIB. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979274.

Entry information

Entry name

CLA4_YEAST

Accession

Primary (citable) accession number: P48562
Secondary accession number(s): D6W0P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents