Reviewed, UniProtKB/Swiss-Prot P48562 (CLA4_YEAST)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase CLA4
    EC=2.7.11.1

Gene names

Name:	CLA4
Ordered Locus Names:	YNL298W
ORF Names:	N0450

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in budding and cytokinesis.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with CDC42.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 PH domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Rho protein signal transduction Traceable author statement. Source: SGD budding cell apical bud growth Traceable author statement. Source: SGD cytokinesis Traceable author statement. Source: SGD establishment of cell polarity Traceable author statement. Source: SGD protein amino acid phosphorylation Traceable author statement. Source: SGD regulation of exit from mitosis Inferred from mutant phenotype. Source: SGD response to pheromone Inferred from genetic interaction. Source: SGD vacuole inheritance Inferred from genetic interaction. Source: SGD
Cellular component	actin cap Traceable author statement. Source: SGD cellular bud Inferred from direct assay. Source: SGD fungal-type vacuole Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MYO5	Q04439	1	EBI-4750,EBI-11687
SAM50	P53969	1	EBI-4750,EBI-28646
STE4	P18851	1	EBI-4750,EBI-7390

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 842	842	Serine/threonine-protein kinase CLA4		PRO_0000085865
Regions
	Domain	61 – 179	119	PH
	Domain	184 – 197	14	CRIB
	Domain	546 – 825	280	Protein kinase
	Nucleotide binding	552 – 560	9	ATP By similarity
	Compositional bias	136 – 144	9	Poly-Ser
	Compositional bias	372 – 387	16	Poly-Gln
Sites
	Active site	693	1	Proton acceptor By similarity
	Binding site	594	1	ATP By similarity
Amino acid modifications
	Modified residue	26	1	Phosphoserine Ref.6 Ref.7
	Modified residue	27	1	Phosphoserine Ref.7
	Modified residue	29	1	Phosphoserine Ref.7
	Modified residue	46	1	Phosphoserine Ref.7
	Modified residue	186	1	Phosphoserine Ref.7
	Modified residue	351	1	Phosphoserine Ref.6 Ref.7 Ref.5
	Modified residue	367	1	Phosphoserine Ref.4
	Modified residue	370	1	Phosphoserine Ref.4
	Modified residue	425	1	Phosphoserine Ref.7
	Modified residue	445	1	Phosphoserine Ref.6 Ref.7
Experimental info
	Sequence conflict	390	1	Y → I in CAA57879. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48562-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 3A2AB86414561DC6	FASTA	842	93,909
	10 20 30 40 50 60 MSLSAAANKI SDNDFQNIGP APRPPSSNSQ GRTCYNQTQP ITKLMSQLDL TSASHLGTST 70 80 90 100 110 120 SKKKSGWVSY KDDGILSFIW QKRYLMLHDS YVALYKNDKQ NDDAILKIPL TSIISVSRTQ 130 140 150 160 170 180 LKQYCFELVR CSDRNSVSSG SSSSLNVSSD SNSKKSIYIA TKTESDLHSW LDAIFAKCPL 190 200 210 220 230 240 LSGVSSPTNF THKVHVGFDP ETGSFVGMPT NWEKLLKHSR ITGEDWNNNS AAVIQVLQFY 250 260 270 280 290 300 QEYNGAGNPT NTLDKPQSGE TSSSQKSLPN SYNDNKLRNN SVNSKSSSGV SSSMVSQRKT 310 320 330 340 350 360 SQPPNTKSPV SLGSGSLPPI NTKLPTSQSN IPRHLQNVPN QQYPKMRNGH SPTNGQFPRG 370 380 390 400 410 420 PMHPNNSQRS LQQQQQQQQQ QKQQHQQYPY HHQGPSPSPS PSPSPLNPYR PHHNMINPYS 430 440 450 460 470 480 KQPQSPLSSQ STQNQAIPRY AQNSSPTAAH FQPQRTAPKP PISAPRAPYP SNQNATSNTH 490 500 510 520 530 540 VQPVAPKNDQ STPQTMRQAP KRPDADVAQP GGVAKPKKPA RPTMSTAEIM SKLKKVTVNA 550 560 570 580 590 600 DPSQCFKVIE KAGQGASGSV YLAERTHIPT ESNMIELINN DIDEPHVGDK VAIKQMVLSK 610 620 630 640 650 660 QPRKELIVNE ILVMKDSRHK NIVNFLEAYL RTDDDLWVVM EFMEGGSLTD IIENSPTNDN 670 680 690 700 710 720 SHSPLTEPQI AYIVRETCQG LKFLHDKHII HRDIKSDNVL LDTRARVKIT DFGFCARLTD 730 740 750 760 770 780 KRSKRATMVG TPYWMAPEVV KQREYDEKID VWSLGIMTIE MLEGEPPYLN EDPLKALYLI 790 800 810 820 830 840 ATNGTPKLKH PESLSLEIKR FLSVCLCVDV RYRASTEELL HHGFFNMACD PKDLTSLLEW KE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast." Cvrckova F., de Virgilio C., Manser E., Pringle J.R., Nasmyth K. Genes Dev. 9:1817-1830(1995) [PubMed: 7649470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K1107.
[2]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-367 AND SER-370, MASS SPECTROMETRY.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-351 AND SER-445, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-27; SER-29; SER-46; SER-186; SER-351; SER-425 AND SER-445, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X82499 Genomic DNA. Translation: CAA57879.1. U23084 Genomic DNA. Translation: AAC49100.1. Z71574 Genomic DNA. Translation: CAA96216.1.
PIR	S60402.
RefSeq	NP_014101.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2276N.
IntAct	P48562. 31 interactions.
STRING	P48562.
Proteomic databases
PRIDE	P48562.
Genome annotation databases
Ensembl	YNL298W; YNL298W; YNL298W; Saccharomyces cerevisiae. [Genome view]
GeneID	855418.
KEGG	sce:YNL298W.
NMPDR	fig|4932.3.peg.5163.
Organism-specific databases
CYGD	YNL298w.
SGD	S000005242. CLA4.
Phylogenomic databases
HOGENOM	P48562.
OMA	CVDVRYR
OrthoDB	EOG9DBVX9
Enzyme and pathway databases
BRENDA	2.7.11.1. 250.
Gene expression databases
ArrayExpress	P48562.
Genevestigator	P48562.
GermOnline	YNL298W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000095. PAK_box_Rho_bd. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_prot_kinase-like_dom. IPR015750. Ser/Thr_kinase_Pak-rel. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR22986:SF84. Pak_like. 1 hit.
Pfam	PF00786. PBD. 1 hit. PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00285. PBD. 1 hit. SM00233. PH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS50108. CRIB. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979274.

Entry information

Entry name

CLA4_YEAST

Accession

Primary (citable) accession number: P48562

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)