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Protein

Serine/threonine-protein kinase CLA4

Gene

CLA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in budding and cytokinesis.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594ATPPROSITE-ProRule annotation1
Active sitei693Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi552 – 560ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: SGD
  • Rac GTPase binding Source: GO_Central

GO - Biological processi

  • actin cytoskeleton organization Source: GO_Central
  • activation of protein kinase activity Source: GO_Central
  • negative regulation of gene expression Source: SGD
  • negative regulation of sterol import by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of protein import into nucleus Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of apoptotic process Source: GO_Central
  • regulation of exit from mitosis Source: SGD
  • regulation of MAPK cascade Source: GO_Central
  • response to pheromone Source: SGD
  • Rho protein signal transduction Source: GO_Central
  • septin ring organization Source: SGD
  • signal transduction by protein phosphorylation Source: GO_Central
  • sterol import Source: SGD
  • stress-activated protein kinase signaling cascade Source: GO_Central
  • vacuole inheritance Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33286-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-2559580. Oxidative Stress Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase CLA4 (EC:2.7.11.1)
Gene namesi
Name:CLA4
Ordered Locus Names:YNL298W
ORF Names:N0450
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL298W.
SGDiS000005242. CLA4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858651 – 842Serine/threonine-protein kinase CLA4Add BLAST842

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineCombined sources1
Modified residuei46PhosphoserineCombined sources1
Modified residuei351PhosphoserineCombined sources1
Modified residuei367PhosphoserineCombined sources1
Modified residuei425PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48562.
PRIDEiP48562.

PTM databases

iPTMnetiP48562.

Interactioni

Subunit structurei

Interacts with CDC42.

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35540. 721 interactors.
DIPiDIP-2276N.
IntActiP48562. 42 interactors.
MINTiMINT-582371.
STRINGi4932.YNL298W.

Structurei

3D structure databases

ProteinModelPortaliP48562.
SMRiP48562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 179PHPROSITE-ProRule annotationAdd BLAST119
Domaini184 – 197CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini546 – 825Protein kinasePROSITE-ProRule annotationAdd BLAST280

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 144Poly-Ser9
Compositional biasi372 – 387Poly-GlnAdd BLAST16

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00890000139619.
HOGENOMiHOG000234202.
InParanoidiP48562.
KOiK19833.
OMAiFQNIGPA.
OrthoDBiEOG092C2E30.

Family and domain databases

CDDicd01093. CRIB_PAK_like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiView protein in InterPro
IPR000095. CRIB_dom.
IPR036936. CRIB_dom_sf.
IPR011009. Kinase-like_dom.
IPR033923. PAK_BD.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00786. PBD. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P48562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSAAANKI SDNDFQNIGP APRPPSSNSQ GRTCYNQTQP ITKLMSQLDL
60 70 80 90 100
TSASHLGTST SKKKSGWVSY KDDGILSFIW QKRYLMLHDS YVALYKNDKQ
110 120 130 140 150
NDDAILKIPL TSIISVSRTQ LKQYCFELVR CSDRNSVSSG SSSSLNVSSD
160 170 180 190 200
SNSKKSIYIA TKTESDLHSW LDAIFAKCPL LSGVSSPTNF THKVHVGFDP
210 220 230 240 250
ETGSFVGMPT NWEKLLKHSR ITGEDWNNNS AAVIQVLQFY QEYNGAGNPT
260 270 280 290 300
NTLDKPQSGE TSSSQKSLPN SYNDNKLRNN SVNSKSSSGV SSSMVSQRKT
310 320 330 340 350
SQPPNTKSPV SLGSGSLPPI NTKLPTSQSN IPRHLQNVPN QQYPKMRNGH
360 370 380 390 400
SPTNGQFPRG PMHPNNSQRS LQQQQQQQQQ QKQQHQQYPY HHQGPSPSPS
410 420 430 440 450
PSPSPLNPYR PHHNMINPYS KQPQSPLSSQ STQNQAIPRY AQNSSPTAAH
460 470 480 490 500
FQPQRTAPKP PISAPRAPYP SNQNATSNTH VQPVAPKNDQ STPQTMRQAP
510 520 530 540 550
KRPDADVAQP GGVAKPKKPA RPTMSTAEIM SKLKKVTVNA DPSQCFKVIE
560 570 580 590 600
KAGQGASGSV YLAERTHIPT ESNMIELINN DIDEPHVGDK VAIKQMVLSK
610 620 630 640 650
QPRKELIVNE ILVMKDSRHK NIVNFLEAYL RTDDDLWVVM EFMEGGSLTD
660 670 680 690 700
IIENSPTNDN SHSPLTEPQI AYIVRETCQG LKFLHDKHII HRDIKSDNVL
710 720 730 740 750
LDTRARVKIT DFGFCARLTD KRSKRATMVG TPYWMAPEVV KQREYDEKID
760 770 780 790 800
VWSLGIMTIE MLEGEPPYLN EDPLKALYLI ATNGTPKLKH PESLSLEIKR
810 820 830 840
FLSVCLCVDV RYRASTEELL HHGFFNMACD PKDLTSLLEW KE
Length:842
Mass (Da):93,909
Last modified:February 1, 1996 - v1
Checksum:i3A2AB86414561DC6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti390Y → I in CAA57879 (PubMed:7649470).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82499 Genomic DNA. Translation: CAA57879.1.
U23084 Genomic DNA. Translation: AAC49100.1.
Z71574 Genomic DNA. Translation: CAA96216.1.
BK006947 Genomic DNA. Translation: DAA10262.1.
PIRiS60402.
RefSeqiNP_014101.1. NM_001183136.1.

Genome annotation databases

EnsemblFungiiYNL298W; YNL298W; YNL298W.
GeneIDi855418.
KEGGisce:YNL298W.

Similar proteinsi

Entry informationi

Entry nameiCLA4_YEAST
AccessioniPrimary (citable) accession number: P48562
Secondary accession number(s): D6W0P6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 25, 2017
This is version 183 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names