Reviewed, UniProtKB/Swiss-Prot P48560 (YN40_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized protein YNL300W

Gene names

Ordered Locus Names:	YNL300W
ORF Names:	N0430

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	102 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Secreted › cell wall Probable. Membrane; Lipid-anchor › GPI-anchor Potential.
Post-translational modification	The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.
Miscellaneous	Present with 1480 molecules/cell in log phase SD medium. Ref.5

Ontologies

Keywords
Cellular component	Cell wall Membrane Secreted
Domain	Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 80	62	Uncharacterized protein YNL300W		PRO_0000203371
	Propeptide	81 – 102	22	Removed in mature form Potential		PRO_0000372450
Regions
	Compositional bias	21 – 77	57	Thr-rich
Amino acid modifications
	Lipidation	80	1	GPI-anchor amidated glycine Potential
	Glycosylation	69	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P48560-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 2F8D02993704E1F5	FASTA	102	10,170
	10 20 30 40 50 60 MKFSTLSTVA AIAAFASADS TSDGVTYVDV TTTPQSTTSM VSTVKTTSTP YTTSTIATLS 70 80 90 100 TKSISSQANT TTHEISTYVG AAVKGSVAGM GAIMGAAAFA LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae." Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K. Mol. Gen. Genet. 258:53-59(1998) [PubMed: 9613572] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23084 Genomic DNA. Translation: AAC49098.1. Z71576 Genomic DNA. Translation: CAA96218.1. AY692649 Genomic DNA. Translation: AAT92668.1.
PIR	S60400.
RefSeq	NP_014099.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4464N.
IntAct	P48560. 1 interaction.
STRING	P48560.
Genome annotation databases
Ensembl	YNL300W; YNL300W; YNL300W; Saccharomyces cerevisiae. [Genome view]
GeneID	855416.
KEGG	sce:YNL300W.
NMPDR	fig|4932.3.peg.5161.
Organism-specific databases
CYGD	YNL300w.
SGD	S000005244. YNL300W.
Phylogenomic databases
OMA	ANTTTHE.
OrthoDB	EOG9MW9PD.
Gene expression databases
ArrayExpress	P48560.
Genevestigator	P48560.
GermOnline	YNL300W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020514. Uncharacterised_YNL300W. [Graphical view]
ProDom	PD068652. Uncharacterised_YNL300W. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	979268.

Entry information

Entry name

YN40_YEAST

Accession

Primary (citable) accession number: P48560

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)