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P48559

- YPT11_YEAST

UniProt

P48559 - YPT11_YEAST

Protein

GTP-binding protein YPT11

Gene

YPT11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Involved in the positive control of both endoplasmic reticulum (ER) and mitochondrion inheritance during cell divison. Required for the MYO2-dependent retention of newly inherited mitochondria at the bud tip in developing daughter cells.5 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi97 – 1048GTPSequence Analysis
    Nucleotide bindingi228 – 2325GTPSequence Analysis
    Nucleotide bindingi292 – 2954GTPSequence Analysis

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. endoplasmic reticulum inheritance Source: SGD
    2. Golgi inheritance Source: SGD
    3. Golgi localization Source: SGD
    4. mitochondrion inheritance Source: SGD
    5. mitochondrion localization Source: SGD
    6. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33291-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein YPT11
    Alternative name(s):
    Rab GTPase YPT11
    Gene namesi
    Name:YPT11
    Ordered Locus Names:YNL304W
    ORF Names:N0410
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    SGDiS000005248. YPT11.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication. Bud tip 1 Publication. Bud neck 1 Publication
    Note: Enriched in the peripheral ER of small buds and daughter cells. Concentrates at the site of bud emergence, at the bud tip of the growing bud, and at the bud neck during M phase. Interaction with MYO2 is required for proper localization to the bud.

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. endoplasmic reticulum Source: SGD
    4. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    5. incipient cellular bud site Source: SGD
    6. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi102 – 1021G → D: Impairs interaction with MYO2. 1 Publication
    Mutagenesisi206 – 2061I → N: Abolishes interaction with MYO2. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 417417GTP-binding protein YPT11PRO_0000121322Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi415 – 4151S-geranylgeranyl cysteineBy similarity
    Lipidationi416 – 4161S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Prenylation

    Proteomic databases

    MaxQBiP48559.
    PaxDbiP48559.

    Expressioni

    Gene expression databases

    GenevestigatoriP48559.

    Interactioni

    Subunit structurei

    Interacts with MYO2 (via C-terminal tail domain). Interacts with YIF1, YIP3, YIP4 and YIP5.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YIP3P536332EBI-29362,EBI-25301
    YIP4P530932EBI-29362,EBI-24124

    Protein-protein interaction databases

    BioGridi35535. 29 interactions.
    DIPiDIP-7864N.
    IntActiP48559. 8 interactions.
    MINTiMINT-2492537.
    STRINGi4932.YNL304W.

    Structurei

    3D structure databases

    ProteinModelPortaliP48559.
    SMRiP48559. Positions 202-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000141968.
    KOiK17977.
    OMAiCMERWIV.
    OrthoDBiEOG7KM64Z.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48559-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQRKRYSLN VVTSPSIPSP TPSAPIRTNE SNWEAASPAS AASSFLPNVH    50
    HGGTVLNPGL GIMRSPSLNK SGAFGRSGSS GSSTVIEPSN IKLLLIGDAN 100
    VGKTAMILSY CRELLTRAEM SRSARLRHQQ QQQHKDLGLK KTVVNHRLSM 150
    KEKRKRYSSN DFEKEFKDIN HFADETSDFG NPNIGDDNNH EMADPNEIVI 200
    ETRSTIGIDI KTNLVNIDNR FFNVILWDTA GQERYQNAII PSLYKKTNAV 250
    ILTYDITNAK SFQSCMERWI VQALENFSSQ DLLKARFFLV GNKIDLYKER 300
    QVTHYDVVQM VQEMQLKHGI KISGNFEVSC KWVNVVERTM NMIILDLVEN 350
    GCFENNDPCV SITTSDDVQG HEQEFHDTVE EPFNFTRQRQ HQLEKNNTVD 400
    ITKPNDDIAN NQSICCV 417
    Length:417
    Mass (Da):47,116
    Last modified:June 16, 2009 - v2
    Checksum:i26055FCE32A64977
    GO

    Sequence cautioni

    The sequence AAC49094.1 differs from that shown. Reason: Frameshift at position 36.
    The sequence CAA96231.1 differs from that shown. Reason: Frameshift at position 36.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23084 Genomic DNA. Translation: AAC49094.1. Frameshift.
    Z71580 Genomic DNA. Translation: CAA96231.1. Frameshift.
    BK006947 Genomic DNA. Translation: DAA10257.1.
    PIRiS60396.
    RefSeqiNP_014095.2. NM_001183142.1.

    Genome annotation databases

    EnsemblFungiiYNL304W; YNL304W; YNL304W.
    GeneIDi855412.
    KEGGisce:YNL304W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23084 Genomic DNA. Translation: AAC49094.1 . Frameshift.
    Z71580 Genomic DNA. Translation: CAA96231.1 . Frameshift.
    BK006947 Genomic DNA. Translation: DAA10257.1 .
    PIRi S60396.
    RefSeqi NP_014095.2. NM_001183142.1.

    3D structure databases

    ProteinModelPortali P48559.
    SMRi P48559. Positions 202-331.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35535. 29 interactions.
    DIPi DIP-7864N.
    IntActi P48559. 8 interactions.
    MINTi MINT-2492537.
    STRINGi 4932.YNL304W.

    Proteomic databases

    MaxQBi P48559.
    PaxDbi P48559.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL304W ; YNL304W ; YNL304W .
    GeneIDi 855412.
    KEGGi sce:YNL304W.

    Organism-specific databases

    SGDi S000005248. YPT11.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000141968.
    KOi K17977.
    OMAi CMERWIV.
    OrthoDBi EOG7KM64Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33291-MONOMER.

    Miscellaneous databases

    NextBioi 979259.

    Gene expression databases

    Genevestigatori P48559.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene."
      Maurer K.C.T., Urbanus J.H.M., Planta R.J.
      Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
      Calero M., Collins R.N.
      Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIP3.
    5. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
      Calero M., Winand N.J., Collins R.N.
      FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
    6. "Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae."
      Itoh T., Watabe A., Toh-e A., Matsui Y.
      Mol. Cell. Biol. 22:7744-7757(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MYO2, MUTAGENESIS OF GLY-102 AND ILE-206.
    7. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF FRAMESHIFT.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
      Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
      Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF FRAMESHIFT.
    10. "Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast."
      Itoh T., Toh-e A., Matsui Y.
      EMBO J. 23:2520-2530(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MITOCHONDRION INHERITANCE.
    11. "A type V myosin (Myo2p) and a Rab-like G-protein (Ypt11p) are required for retention of newly inherited mitochondria in yeast cells during cell division."
      Boldogh I.R., Ramcharan S.L., Yang H.-C., Pon L.A.
      Mol. Biol. Cell 15:3994-4002(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MITOCHONDRION INHERITANCE.
    12. "Bioinformatic and comparative localization of Rab proteins reveals functional insights into the uncharacterized GTPases Ypt10p and Ypt11p."
      Buvelot Frei S., Rahl P.B., Nussbaum M., Briggs B.J., Calero M., Janeczko S., Regan A.D., Chen C.Z., Barral Y., Whittaker G.R., Collins R.N.
      Mol. Cell. Biol. 26:7299-7317(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ENDOPLASMIC RETICULUM INHERITANCE, SUBCELLULAR LOCATION.
    13. "Multiple pathways influence mitochondrial inheritance in budding yeast."
      Frederick R.L., Okamoto K., Shaw J.M.
      Genetics 178:825-837(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ENDOPLASMIC RETICULUM AND MITOCHONDRION INHERITANCE.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYPT11_YEAST
    AccessioniPrimary (citable) accession number: P48559
    Secondary accession number(s): D6W0P1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2850 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3