Reviewed, UniProtKB/Swiss-Prot P48559 (YPT11_YEAST)

Names and origin

Protein names

Recommended name:
    GTP-binding protein YPT11
Alternative name(s):
    Rab GTPase YPT11

Gene names

Name:	YPT11
Ordered Locus Names:	YNL304W
ORF Names:	N0410

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the positive control of both endoplasmic reticulum (ER) and mitochondrion inheritance during cell divison. Required for the MYO2-dependent retention of newly inherited mitochondria at the bud tip in developing daughter cells. Ref.5 Ref.9 Ref.10 Ref.11 Ref.12
Subunit structure	Interacts with MYO2 (via C-terminal tail domain). Interacts with YIF1, YIP3, YIP4 and YIP5. Ref.5 Ref.3 Ref.4
Subcellular location	Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Bud tip. Bud neck. Note: Enriched in the peripheral ER of small buds and daughter cells. Concentrates at the site of bud emergence, at the bud tip of the growing bud, and at the bud neck during M phase. Interaction with MYO2 is required for proper localization to the bud. Ref.11
Miscellaneous	Present with 2850 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.
Sequence caution	The sequence AAC49094.1 differs from that shown. Reason: Frameshift at position 36. The sequence CAA96231.1 differs from that shown. Reason: Frameshift at position 36.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Phosphoprotein Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Golgi inheritance Inferred from genetic interaction. Source: SGD Golgi localization Inferred from genetic interaction. Source: SGD endoplasmic reticulum inheritance Ref.11 Inferred from mutant phenotype. Source: SGD mitochondrion inheritance Ref.5 Ref.12 Inferred from genetic interaction. Source: SGD
Cellular component	cellular bud neck Ref.5 Inferred from direct assay. Source: SGD cellular bud tip Ref.5 Inferred from direct assay. Source: SGD endoplasmic reticulum Ref.11 Inferred from direct assay. Source: SGD incipient cellular bud site Ref.5 Inferred from direct assay. Source: SGD membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Ref.5 Inferred from physical interaction. Source: SGD protein binding Ref.3 Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ESS1	P22696	1	EBI-29362,EBI-6679
PRP40	P33203	1	EBI-29362,EBI-701
SET2	P46995	1	EBI-29362,EBI-16985
YIF1	P53845	1	EBI-29362,EBI-28230
YIP3	P53633	1	EBI-29362,EBI-25301
YIP4	P53093	1	EBI-29362,EBI-24124

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	GTP-binding protein YPT11		PRO_0000121322
Regions
	Nucleotide binding	97 – 104	8	GTP Potential
	Nucleotide binding	228 – 232	5	GTP Potential
	Nucleotide binding	292 – 295	4	GTP Potential
Amino acid modifications
	Modified residue	79	1	Phosphoserine Ref.13
	Modified residue	158	1	Phosphoserine Ref.13
	Lipidation	415	1	S-geranylgeranyl cysteine By similarity
	Lipidation	416	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	102	1	G → D: Impairs interaction with MYO2. Ref.5
	Mutagenesis	206	1	I → N: Abolishes interaction with MYO2. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P48559-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 26055FCE32A64977	FASTA	417	47,116
	10 20 30 40 50 60 MSQRKRYSLN VVTSPSIPSP TPSAPIRTNE SNWEAASPAS AASSFLPNVH HGGTVLNPGL 70 80 90 100 110 120 GIMRSPSLNK SGAFGRSGSS GSSTVIEPSN IKLLLIGDAN VGKTAMILSY CRELLTRAEM 130 140 150 160 170 180 SRSARLRHQQ QQQHKDLGLK KTVVNHRLSM KEKRKRYSSN DFEKEFKDIN HFADETSDFG 190 200 210 220 230 240 NPNIGDDNNH EMADPNEIVI ETRSTIGIDI KTNLVNIDNR FFNVILWDTA GQERYQNAII 250 260 270 280 290 300 PSLYKKTNAV ILTYDITNAK SFQSCMERWI VQALENFSSQ DLLKARFFLV GNKIDLYKER 310 320 330 340 350 360 QVTHYDVVQM VQEMQLKHGI KISGNFEVSC KWVNVVERTM NMIILDLVEN GCFENNDPCV 370 380 390 400 410 SITTSDDVQG HEQEFHDTVE EPFNFTRQRQ HQLEKNNTVD ITKPNDDIAN NQSICCV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins." Calero M., Collins R.N. Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed: 11785952] [Abstract] Cited for: INTERACTION WITH YIP3.
[4]	"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors." Calero M., Winand N.J., Collins R.N. FEBS Lett. 515:89-98(2002) [PubMed: 11943201] [Abstract] Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[5]	"Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae." Itoh T., Watabe A., Toh-e A., Matsui Y. Mol. Cell. Biol. 22:7744-7757(2002) [PubMed: 12391144] [Abstract] Cited for: FUNCTION, INTERACTION WITH MYO2, MUTAGENESIS OF GLY-102 AND ILE-206.
[6]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed: 12775844] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[9]	"Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast." Itoh T., Toh-e A., Matsui Y. EMBO J. 23:2520-2530(2004) [PubMed: 15201867] [Abstract] Cited for: FUNCTION IN MITOCHONDRION INHERITANCE.
[10]	"A type V myosin (Myo2p) and a Rab-like G-protein (Ypt11p) are required for retention of newly inherited mitochondria in yeast cells during cell division." Boldogh I.R., Ramcharan S.L., Yang H.-C., Pon L.A. Mol. Biol. Cell 15:3994-4002(2004) [PubMed: 15215313] [Abstract] Cited for: FUNCTION IN MITOCHONDRION INHERITANCE.
[11]	"Bioinformatic and comparative localization of Rab proteins reveals functional insights into the uncharacterized GTPases Ypt10p and Ypt11p." Buvelot Frei S., Rahl P.B., Nussbaum M., Briggs B.J., Calero M., Janeczko S., Regan A.D., Chen C.Z., Barral Y., Whittaker G.R., Collins R.N. Mol. Cell. Biol. 26:7299-7317(2006) [PubMed: 16980630] [Abstract] Cited for: FUNCTION IN ENDOPLASMIC RETICULUM INHERITANCE, SUBCELLULAR LOCATION.
[12]	"Multiple pathways influence mitochondrial inheritance in budding yeast." Frederick R.L., Okamoto K., Shaw J.M. Genetics 178:825-837(2008) [PubMed: 18245340] [Abstract] Cited for: FUNCTION IN ENDOPLASMIC RETICULUM AND MITOCHONDRION INHERITANCE.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79 AND SER-158, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23084 Genomic DNA. Translation: AAC49094.1. Frameshift. Z71580 Genomic DNA. Translation: CAA96231.1. Frameshift.
PIR	S60396.
RefSeq	NP_014095.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:7864N.
IntAct	P48559. 8 interactions.
STRING	P48559.
Genome annotation databases
Ensembl	YNL304W; YNL304W; YNL304W; Saccharomyces cerevisiae. [Genome view]
GeneID	855412.
KEGG	sce:YNL304W.
NMPDR	fig|4932.3.peg.5157.
Organism-specific databases
CYGD	YNL304w.
SGD	S000005248. YPT11.
Phylogenomic databases
HOGENOM	P48559.
OrthoDB	EOG93R54H
Gene expression databases
ArrayExpress	P48559.
Genevestigator	P48559.
GermOnline	YNL304W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013753. Ras. IPR020851. Small_GTPase. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YPT11_YEAST

Accession

Primary (citable) accession number: P48559

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	June 16, 2009
Last modified:	November 24, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)