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P48559 (YPT11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein YPT11
Alternative name(s):
Rab GTPase YPT11
Gene names
Name:YPT11
Ordered Locus Names:YNL304W
ORF Names:N0410
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the positive control of both endoplasmic reticulum (ER) and mitochondrion inheritance during cell divison. Required for the MYO2-dependent retention of newly inherited mitochondria at the bud tip in developing daughter cells. Ref.6 Ref.10 Ref.11 Ref.12 Ref.13

Subunit structure

Interacts with MYO2 (via C-terminal tail domain). Interacts with YIF1, YIP3, YIP4 and YIP5. Ref.4 Ref.5 Ref.6

Subcellular location

Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Bud tip. Bud neck. Note: Enriched in the peripheral ER of small buds and daughter cells. Concentrates at the site of bud emergence, at the bud tip of the growing bud, and at the bud neck during M phase. Interaction with MYO2 is required for proper localization to the bud. Ref.12

Miscellaneous

Present with 2850 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence caution

The sequence AAC49094.1 differs from that shown. Reason: Frameshift at position 36.

The sequence CAA96231.1 differs from that shown. Reason: Frameshift at position 36.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YIP3P536332EBI-29362,EBI-25301
YIP4P530932EBI-29362,EBI-24124

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417GTP-binding protein YPT11
PRO_0000121322

Regions

Nucleotide binding97 – 1048GTP Potential
Nucleotide binding228 – 2325GTP Potential
Nucleotide binding292 – 2954GTP Potential

Amino acid modifications

Lipidation4151S-geranylgeranyl cysteine By similarity
Lipidation4161S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis1021G → D: Impairs interaction with MYO2. Ref.6
Mutagenesis2061I → N: Abolishes interaction with MYO2. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P48559 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: 26055FCE32A64977

FASTA41747,116
        10         20         30         40         50         60 
MSQRKRYSLN VVTSPSIPSP TPSAPIRTNE SNWEAASPAS AASSFLPNVH HGGTVLNPGL 

        70         80         90        100        110        120 
GIMRSPSLNK SGAFGRSGSS GSSTVIEPSN IKLLLIGDAN VGKTAMILSY CRELLTRAEM 

       130        140        150        160        170        180 
SRSARLRHQQ QQQHKDLGLK KTVVNHRLSM KEKRKRYSSN DFEKEFKDIN HFADETSDFG 

       190        200        210        220        230        240 
NPNIGDDNNH EMADPNEIVI ETRSTIGIDI KTNLVNIDNR FFNVILWDTA GQERYQNAII 

       250        260        270        280        290        300 
PSLYKKTNAV ILTYDITNAK SFQSCMERWI VQALENFSSQ DLLKARFFLV GNKIDLYKER 

       310        320        330        340        350        360 
QVTHYDVVQM VQEMQLKHGI KISGNFEVSC KWVNVVERTM NMIILDLVEN GCFENNDPCV 

       370        380        390        400        410 
SITTSDDVQG HEQEFHDTVE EPFNFTRQRQ HQLEKNNTVD ITKPNDDIAN NQSICCV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene."
Maurer K.C.T., Urbanus J.H.M., Planta R.J.
Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
Calero M., Collins R.N.
Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIP3.
[5]"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
Calero M., Winand N.J., Collins R.N.
FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[6]"Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae."
Itoh T., Watabe A., Toh-e A., Matsui Y.
Mol. Cell. Biol. 22:7744-7757(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MYO2, MUTAGENESIS OF GLY-102 AND ILE-206.
[7]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[10]"Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast."
Itoh T., Toh-e A., Matsui Y.
EMBO J. 23:2520-2530(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN MITOCHONDRION INHERITANCE.
[11]"A type V myosin (Myo2p) and a Rab-like G-protein (Ypt11p) are required for retention of newly inherited mitochondria in yeast cells during cell division."
Boldogh I.R., Ramcharan S.L., Yang H.-C., Pon L.A.
Mol. Biol. Cell 15:3994-4002(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN MITOCHONDRION INHERITANCE.
[12]"Bioinformatic and comparative localization of Rab proteins reveals functional insights into the uncharacterized GTPases Ypt10p and Ypt11p."
Buvelot Frei S., Rahl P.B., Nussbaum M., Briggs B.J., Calero M., Janeczko S., Regan A.D., Chen C.Z., Barral Y., Whittaker G.R., Collins R.N.
Mol. Cell. Biol. 26:7299-7317(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ENDOPLASMIC RETICULUM INHERITANCE, SUBCELLULAR LOCATION.
[13]"Multiple pathways influence mitochondrial inheritance in budding yeast."
Frederick R.L., Okamoto K., Shaw J.M.
Genetics 178:825-837(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ENDOPLASMIC RETICULUM AND MITOCHONDRION INHERITANCE.
[14]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U23084 Genomic DNA. Translation: AAC49094.1. Frameshift.
Z71580 Genomic DNA. Translation: CAA96231.1. Frameshift.
BK006947 Genomic DNA. Translation: DAA10257.1.
PIRS60396.
RefSeqNP_014095.2. NM_001183142.1.

3D structure databases

ProteinModelPortalP48559.
SMRP48559. Positions 202-331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35535. 29 interactions.
DIPDIP-7864N.
IntActP48559. 8 interactions.
MINTMINT-2492537.
STRING4932.YNL304W.

Proteomic databases

MaxQBP48559.
PaxDbP48559.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL304W; YNL304W; YNL304W.
GeneID855412.
KEGGsce:YNL304W.

Organism-specific databases

SGDS000005248. YPT11.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000141968.
KOK17977.
OMACMERWIV.
OrthoDBEOG7KM64Z.

Enzyme and pathway databases

BioCycYEAST:G3O-33291-MONOMER.

Gene expression databases

GenevestigatorP48559.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979259.

Entry information

Entry nameYPT11_YEAST
AccessionPrimary (citable) accession number: P48559
Secondary accession number(s): D6W0P1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: June 16, 2009
Last modified: June 11, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families