Reviewed, UniProtKB/Swiss-Prot P48558 (YN45_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized vacuolar membrane protein YNL305C

Gene names

Ordered Locus Names:	YNL305C
ORF Names:	N0405

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location

Vacuole membrane; Multi-pass membrane protein Ref.4.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	fungal-type vacuole Ref.4 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Uncharacterized vacuolar membrane protein YNL305C		PRO_0000203369
Regions
	Topological domain	1 – 53	53	Vacuolar Potential
	Transmembrane	54 – 74	21	Potential
	Topological domain	75 – 85	11	Cytoplasmic Potential
	Transmembrane	86 – 106	21	Potential
	Topological domain	107 – 146	40	Vacuolar Potential
	Transmembrane	147 – 167	21	Potential
	Topological domain	168 – 171	4	Cytoplasmic Potential
	Transmembrane	172 – 192	21	Potential
	Topological domain	193 – 208	16	Vacuolar Potential
	Transmembrane	209 – 229	21	Potential
	Topological domain	230 – 239	10	Cytoplasmic Potential
	Transmembrane	240 – 260	21	Potential
	Topological domain	261 – 270	10	Vacuolar Potential
	Transmembrane	271 – 291	21	Potential
	Topological domain	292 – 297	6	Cytoplasmic Potential
Experimental info
	Sequence conflict	84	1	S → A in AAT92698. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P48558-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 330784DA17152BB0	FASTA	297	33,645
	10 20 30 40 50 60 MSGPPPPYEE QSSHLYGQPA SSQDGNAFIP EDFKYSTVVI SCEPIIRQRF MHKVYSLLSC 70 80 90 100 110 120 QLLASLSFCY WASVSTSLQN FIMSHIALFY ICMVVSLVSC IWLAVSPRPE DYEASVPEPL 130 140 150 160 170 180 LTGSSEEPAQ EQRRLPWYVL SSYKQKLTLL SIFTLSEAYC LSLVTLAYDK DTVLSALLIT 190 200 210 220 230 240 TIVVVGVSLT ALSERFENVL NSATSIYYWL NWGLWIMIGM GLTALLFGWN THSSKFNLLY 250 260 270 280 290 GWLGAILFTA YLFIDTQLIF RKVYPDEEVR CAMMLYLDIV NLFLSILRIL ANSNDDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23084 Genomic DNA. Translation: AAC49093.1. Z71581 Genomic DNA. Translation: CAA96233.1. AY692679 Genomic DNA. Translation: AAT92698.1.
PIR	S63281.
RefSeq	NP_014094.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P48558. 2 interactions.
STRING	P48558.
Genome annotation databases
Ensembl	YNL305C; YNL305C; YNL305C; Saccharomyces cerevisiae. [Genome view]
GeneID	855411.
KEGG	sce:YNL305C.
NMPDR	fig|4932.3.peg.5156.
Organism-specific databases
CYGD	YNL305c.
SGD	S000005249. YNL305C.
Phylogenomic databases
HOGENOM	HBG595196.
OMA	DVASCEL.
OrthoDB	EOG9MKPZ9.
Gene expression databases
ArrayExpress	P48558.
Genevestigator	P48558.
GermOnline	YNL305C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006214. UPF0005. [Graphical view]
PANTHER	PTHR23291. UPF0005. 1 hit.
Pfam	PF01027. UPF0005. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979256.

Entry information

Entry name

YN45_YEAST

Accession

Primary (citable) accession number: P48558
Secondary accession number(s): Q6B2Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)