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Protein

Bax inhibitor 1

Gene

BXI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Links the unfolded protein response and programmed cell death and mediates mitochondrial-dependent apoptosis (PubMed:21673659, PubMed:21673967). Induces cell death and disruption of the mitochondrial transmembrane potential via the mitochondrial phosphate carrier MIR1 (PubMed:21673659). Dispensible for starvation-induced autophagy (PubMed:21926971).3 Publications

GO - Biological processi

  • apoptotic process Source: SGD
  • calcium-mediated signaling Source: SGD
  • endoplasmic reticulum unfolded protein response Source: SGD
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BioCyciYEAST:G3O-33292-MONOMER.

Protein family/group databases

TCDBi1.A.14.3.5. the testis-enhanced gene transfer (tegt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Bax inhibitor 1
Alternative name(s):
BH3 domain-containing protein BXI1
Gene namesi
Name:BXI1
Synonyms:YBH3
Ordered Locus Names:YNL305C
ORF Names:N0405
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL305C.
SGDiS000005249. BXI1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5353LumenalSequence analysisAdd
BLAST
Transmembranei54 – 7421HelicalSequence analysisAdd
BLAST
Topological domaini75 – 8511CytoplasmicSequence analysisAdd
BLAST
Transmembranei86 – 10621HelicalSequence analysisAdd
BLAST
Topological domaini107 – 14640LumenalSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Topological domaini168 – 1714CytoplasmicSequence analysis
Transmembranei172 – 19221HelicalSequence analysisAdd
BLAST
Topological domaini193 – 20816LumenalSequence analysisAdd
BLAST
Transmembranei209 – 22921HelicalSequence analysisAdd
BLAST
Topological domaini230 – 23910CytoplasmicSequence analysis
Transmembranei240 – 26021HelicalSequence analysisAdd
BLAST
Topological domaini261 – 27010LumenalSequence analysis
Transmembranei271 – 29121HelicalSequence analysisAdd
BLAST
Topological domaini292 – 2976CytoplasmicSequence analysis

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • fungal-type vacuole Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
  • vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Vacuole

Pathology & Biotechi

Disruption phenotypei

No effect on autophagy during nitrogen starvation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Bax inhibitor 1PRO_0000203369Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi35534. 20 interactions.
IntActiP48558. 15 interactions.
MINTiMINT-4500899.

Structurei

3D structure databases

ProteinModelPortaliP48558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BI1 family. LFG subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00500000044791.
HOGENOMiHOG000060393.
InParanoidiP48558.
KOiK06890.
OMAiFITETHR.
OrthoDBiEOG7RNKBD.

Family and domain databases

InterProiIPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERiPTHR23291. PTHR23291. 2 hits.

Sequencei

Sequence statusi: Complete.

P48558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGPPPPYEE QSSHLYGQPA SSQDGNAFIP EDFKYSTVVI SCEPIIRQRF
60 70 80 90 100
MHKVYSLLSC QLLASLSFCY WASVSTSLQN FIMSHIALFY ICMVVSLVSC
110 120 130 140 150
IWLAVSPRPE DYEASVPEPL LTGSSEEPAQ EQRRLPWYVL SSYKQKLTLL
160 170 180 190 200
SIFTLSEAYC LSLVTLAYDK DTVLSALLIT TIVVVGVSLT ALSERFENVL
210 220 230 240 250
NSATSIYYWL NWGLWIMIGM GLTALLFGWN THSSKFNLLY GWLGAILFTA
260 270 280 290
YLFIDTQLIF RKVYPDEEVR CAMMLYLDIV NLFLSILRIL ANSNDDN
Length:297
Mass (Da):33,645
Last modified:February 1, 1996 - v1
Checksum:i330784DA17152BB0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841S → A in AAT92698 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23084 Genomic DNA. Translation: AAC49093.1.
Z71581 Genomic DNA. Translation: CAA96233.1.
AY692679 Genomic DNA. Translation: AAT92698.1.
BK006947 Genomic DNA. Translation: DAA10256.1.
PIRiS63281.
RefSeqiNP_014094.1. NM_001183143.1.

Genome annotation databases

EnsemblFungiiYNL305C; YNL305C; YNL305C.
GeneIDi855411.
KEGGisce:YNL305C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23084 Genomic DNA. Translation: AAC49093.1.
Z71581 Genomic DNA. Translation: CAA96233.1.
AY692679 Genomic DNA. Translation: AAT92698.1.
BK006947 Genomic DNA. Translation: DAA10256.1.
PIRiS63281.
RefSeqiNP_014094.1. NM_001183143.1.

3D structure databases

ProteinModelPortaliP48558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35534. 20 interactions.
IntActiP48558. 15 interactions.
MINTiMINT-4500899.

Protein family/group databases

TCDBi1.A.14.3.5. the testis-enhanced gene transfer (tegt) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL305C; YNL305C; YNL305C.
GeneIDi855411.
KEGGisce:YNL305C.

Organism-specific databases

EuPathDBiFungiDB:YNL305C.
SGDiS000005249. BXI1.

Phylogenomic databases

GeneTreeiENSGT00500000044791.
HOGENOMiHOG000060393.
InParanoidiP48558.
KOiK06890.
OMAiFITETHR.
OrthoDBiEOG7RNKBD.

Enzyme and pathway databases

BioCyciYEAST:G3O-33292-MONOMER.

Miscellaneous databases

PROiP48558.

Family and domain databases

InterProiIPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERiPTHR23291. PTHR23291. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene."
    Maurer K.C.T., Urbanus J.H.M., Planta R.J.
    Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "BAX inhibitor-1 regulates autophagy by controlling the IRE1alpha branch of the unfolded protein response."
    Castillo K., Rojas-Rivera D., Lisbona F., Caballero B., Nassif M., Court F.A., Schuck S., Ibar C., Walter P., Sierralta J., Glavic A., Hetz C.
    EMBO J. 30:4465-4478(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "Yeast Bax inhibitor, Bxi1p, is an ER-localized protein that links the unfolded protein response and programmed cell death in Saccharomyces cerevisiae."
    Cebulski J., Malouin J., Pinches N., Cascio V., Austriaco N.
    PLoS ONE 6:E20882-E20882(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiBXI1_YEAST
AccessioniPrimary (citable) accession number: P48558
Secondary accession number(s): D6W0P0, Q6B2Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.