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P48556

- PSMD8_HUMAN

UniProt

P48556 - PSMD8_HUMAN

Protein

26S proteasome non-ATPase regulatory subunit 8

Gene

PSMD8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Necessary for activation of the CDC28 kinase.

    GO - Biological processi

    1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: Reactome
    2. antigen processing and presentation of exogenous peptide antigen via MHC class I Source: Reactome
    3. antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: Reactome
    4. antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
    5. apoptotic process Source: Reactome
    6. cellular nitrogen compound metabolic process Source: Reactome
    7. DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
    8. G1/S transition of mitotic cell cycle Source: Reactome
    9. gene expression Source: Reactome
    10. mitotic cell cycle Source: Reactome
    11. mRNA metabolic process Source: Reactome
    12. negative regulation of apoptotic process Source: Reactome
    13. negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Source: Reactome
    14. positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Source: Reactome
    15. protein polyubiquitination Source: Reactome
    16. regulation of apoptotic process Source: Reactome
    17. regulation of cellular amino acid metabolic process Source: Reactome
    18. regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Source: Reactome
    19. RNA metabolic process Source: Reactome
    20. small molecule metabolic process Source: Reactome
    21. viral process Source: Reactome

    Enzyme and pathway databases

    ReactomeiREACT_111056. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_111178. ER-Phagosome pathway.
    REACT_1156. Orc1 removal from chromatin.
    REACT_118656. Activation of NF-kappaB in B cells.
    REACT_1221. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_13464. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_13565. Regulation of ornithine decarboxylase (ODC).
    REACT_150471. Separation of Sister Chromatids.
    REACT_1614. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_172638. Asymmetric localization of PCP proteins.
    REACT_1949. CDT1 association with the CDC6:ORC:origin complex.
    REACT_200766. degradation of AXIN.
    REACT_200841. degradation of DVL.
    REACT_20549. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_25325. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_4. Ubiquitin-dependent degradation of Cyclin D1.
    REACT_6761. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_6785. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_6821. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_6871. APC/C:Cdc20 mediated degradation of Securin.
    REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_9003. SCF(Skp2)-mediated degradation of p27/p21.
    REACT_9031. Vpu mediated degradation of CD4.
    REACT_9453. Vif-mediated degradation of APOBEC3G.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    26S proteasome non-ATPase regulatory subunit 8
    Alternative name(s):
    26S proteasome regulatory subunit RPN12
    26S proteasome regulatory subunit S14
    p31
    Gene namesi
    Name:PSMD8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:9566. PSMD8.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. extracellular vesicular exosome Source: UniProt
    3. nucleoplasm Source: Reactome
    4. nucleus Source: UniProt
    5. proteasome accessory complex Source: UniProtKB
    6. proteasome complex Source: ProtInc
    7. proteasome regulatory particle Source: ProtInc

    Keywords - Cellular componenti

    Proteasome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33912.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 35035026S proteasome non-ATPase regulatory subunit 8PRO_0000173846Add
    BLAST

    Proteomic databases

    MaxQBiP48556.
    PaxDbiP48556.
    PRIDEiP48556.

    PTM databases

    PhosphoSiteiP48556.

    Expressioni

    Gene expression databases

    ArrayExpressiP48556.
    BgeeiP48556.
    CleanExiHS_PSMD8.
    GenevestigatoriP48556.

    Organism-specific databases

    HPAiCAB021101.
    HPA006702.

    Interactioni

    Protein-protein interaction databases

    BioGridi111686. 67 interactions.
    IntActiP48556. 9 interactions.
    MINTiMINT-1136185.
    STRINGi9606.ENSP00000215071.

    Structurei

    3D structure databases

    ProteinModelPortaliP48556.
    SMRiP48556. Positions 102-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the proteasome subunit S14 family.Curated

    Phylogenomic databases

    eggNOGiNOG285042.
    HOGENOMiHOG000196008.
    HOVERGENiHBG024389.
    InParanoidiP48556.
    KOiK03031.
    OMAiNVYIRHP.
    OrthoDBiEOG7T7GTQ.
    PhylomeDBiP48556.
    TreeFamiTF106233.

    Family and domain databases

    InterProiIPR006746. 26S_Psome_Rpn12.
    [Graphical view]
    PANTHERiPTHR12387. PTHR12387. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P48556-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFIKGRAPRA PPRERRRATR GGLRQVVAPP RALGSTSRPH FRRASVCRRR    50
    CRKSGGLLAA SRKMAAAAVN GAAGFSSSGP AATSGAVLQA ATGMYEQLKG 100
    EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI 150
    GAQWSILRKD IPSFERYMAQ LKCYYFDYKE QLPESAYMHQ LLGLNLLFLL 200
    SQNRVAEFHT ELERLPAKDI QTNVYIKHPV SLEQYLMEGS YNKVFLAKGN 250
    IPAESYTFFI DILLDTIRDE IAGCIEKAYE KILFTEATRI LFFNTPKKMT 300
    DYAKKRGWVL GPNNYYSFAS QQQKPEDTTI PSTELAKQVI EYARQLEMIV 350
    Length:350
    Mass (Da):39,612
    Last modified:October 5, 2010 - v2
    Checksum:iFF20203213333CB3
    GO

    Sequence cautioni

    The sequence AAH01164.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAH65006.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAA07237.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAC62833.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK301771 mRNA. Translation: BAG63230.1.
    AC005789 Genomic DNA. Translation: AAC62833.1. Sequence problems.
    CH471126 Genomic DNA. Translation: EAW56785.1.
    BC001164 mRNA. Translation: AAH01164.3. Different initiation.
    BC065006 mRNA. Translation: AAH65006.2. Different initiation.
    D38047 mRNA. Translation: BAA07237.1. Different initiation.
    CCDSiCCDS12515.2.
    PIRiS56108.
    RefSeqiNP_002803.2. NM_002812.4.
    UniGeneiHs.78466.

    Genome annotation databases

    EnsembliENST00000215071; ENSP00000215071; ENSG00000099341.
    GeneIDi5714.
    KEGGihsa:5714.
    UCSCiuc002oii.4. human.

    Polymorphism databases

    DMDMi308153477.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK301771 mRNA. Translation: BAG63230.1 .
    AC005789 Genomic DNA. Translation: AAC62833.1 . Sequence problems.
    CH471126 Genomic DNA. Translation: EAW56785.1 .
    BC001164 mRNA. Translation: AAH01164.3 . Different initiation.
    BC065006 mRNA. Translation: AAH65006.2 . Different initiation.
    D38047 mRNA. Translation: BAA07237.1 . Different initiation.
    CCDSi CCDS12515.2.
    PIRi S56108.
    RefSeqi NP_002803.2. NM_002812.4.
    UniGenei Hs.78466.

    3D structure databases

    ProteinModelPortali P48556.
    SMRi P48556. Positions 102-343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111686. 67 interactions.
    IntActi P48556. 9 interactions.
    MINTi MINT-1136185.
    STRINGi 9606.ENSP00000215071.

    PTM databases

    PhosphoSitei P48556.

    Polymorphism databases

    DMDMi 308153477.

    Proteomic databases

    MaxQBi P48556.
    PaxDbi P48556.
    PRIDEi P48556.

    Protocols and materials databases

    DNASUi 5714.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000215071 ; ENSP00000215071 ; ENSG00000099341 .
    GeneIDi 5714.
    KEGGi hsa:5714.
    UCSCi uc002oii.4. human.

    Organism-specific databases

    CTDi 5714.
    GeneCardsi GC19P038865.
    H-InvDB HIX0040074.
    HGNCi HGNC:9566. PSMD8.
    HPAi CAB021101.
    HPA006702.
    neXtProti NX_P48556.
    PharmGKBi PA33912.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG285042.
    HOGENOMi HOG000196008.
    HOVERGENi HBG024389.
    InParanoidi P48556.
    KOi K03031.
    OMAi NVYIRHP.
    OrthoDBi EOG7T7GTQ.
    PhylomeDBi P48556.
    TreeFami TF106233.

    Enzyme and pathway databases

    Reactomei REACT_111056. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_111178. ER-Phagosome pathway.
    REACT_1156. Orc1 removal from chromatin.
    REACT_118656. Activation of NF-kappaB in B cells.
    REACT_1221. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_13464. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_13565. Regulation of ornithine decarboxylase (ODC).
    REACT_150471. Separation of Sister Chromatids.
    REACT_1614. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_172638. Asymmetric localization of PCP proteins.
    REACT_1949. CDT1 association with the CDC6:ORC:origin complex.
    REACT_200766. degradation of AXIN.
    REACT_200841. degradation of DVL.
    REACT_20549. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_25325. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_4. Ubiquitin-dependent degradation of Cyclin D1.
    REACT_6761. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    REACT_6785. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_6821. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_6871. APC/C:Cdc20 mediated degradation of Securin.
    REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_9003. SCF(Skp2)-mediated degradation of p27/p21.
    REACT_9031. Vpu mediated degradation of CD4.
    REACT_9453. Vif-mediated degradation of APOBEC3G.

    Miscellaneous databases

    ChiTaRSi PSMD8. human.
    GeneWikii PSMD8.
    GenomeRNAii 5714.
    NextBioi 22198.
    PROi P48556.

    Gene expression databases

    ArrayExpressi P48556.
    Bgeei P48556.
    CleanExi HS_PSMD8.
    Genevestigatori P48556.

    Family and domain databases

    InterProi IPR006746. 26S_Psome_Rpn12.
    [Graphical view ]
    PANTHERi PTHR12387. PTHR12387. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 67-350.
      Tissue: Muscle and Skin.
    5. "Nin1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae."
      Kominami K., DeMartino G.N., Moomaw C., Slaughter C.A., Shimbara N., Fujimuro M., Yokosawa H., Hisamatsu H., Tanahashi N., Shimizu Y., Tanaka K., Toh-e A.
      EMBO J. 14:3105-3115(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 71-350.
    6. "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex."
      Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L.
      Biochemistry 46:3553-3565(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPSMD8_HUMAN
    AccessioniPrimary (citable) accession number: P48556
    Secondary accession number(s): B4DX18, Q6P1L7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-64 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3