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Protein

26S proteasome non-ATPase regulatory subunit 8

Gene

PSMD8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.1 Publication

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-446652. Interleukin-1 family signaling.
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-HSA-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.
R-HSA-8939902. Regulation of RUNX2 expression and activity.
R-HSA-8941858. Regulation of RUNX3 expression and activity.
R-HSA-8948751. Regulation of PTEN stability and activity.
R-HSA-8951664. Neddylation.
R-HSA-9010553. Regulation of expression of SLITs and ROBOs.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 8
Alternative name(s):
26S proteasome regulatory subunit RPN12
26S proteasome regulatory subunit S14
p31
Gene namesi
Name:PSMD8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000099341.11.
HGNCiHGNC:9566. PSMD8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5714.
OpenTargetsiENSG00000099341.
PharmGKBiPA33912.

Chemistry databases

ChEMBLiCHEMBL2364701.

Polymorphism and mutation databases

BioMutaiPSMD8.
DMDMi308153477.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738461 – 35026S proteasome non-ATPase regulatory subunit 8Add BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei106PhosphoserineCombined sources1
Cross-linki297Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP48556.
PaxDbiP48556.
PeptideAtlasiP48556.
PRIDEiP48556.

PTM databases

iPTMnetiP48556.
PhosphoSitePlusiP48556.
SwissPalmiP48556.

Expressioni

Gene expression databases

BgeeiENSG00000099341.
CleanExiHS_PSMD8.
ExpressionAtlasiP48556. baseline and differential.
GenevisibleiP48556. HS.

Organism-specific databases

HPAiCAB021101.
HPA006702.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD8, a base containing 6 ATPases and few additional components.2 Publications

Protein-protein interaction databases

BioGridi111686. 89 interactors.
CORUMiP48556.
IntActiP48556. 26 interactors.
MINTiMINT-1136185.
STRINGi9606.ENSP00000215071.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50T1-350[»]
5GJRelectron microscopy3.507/T1-350[»]
5L4Kelectron microscopy4.50T1-350[»]
5LN3electron microscopy6.80T1-350[»]
5M32electron microscopy3.80r1-350[»]
5T0Celectron microscopy3.80Ad/Bd94-350[»]
5T0Gelectron microscopy4.40d2-350[»]
5T0Helectron microscopy6.80d2-350[»]
5T0Ielectron microscopy8.00d2-350[»]
5T0Jelectron microscopy8.00d2-350[»]
5VGZelectron microscopy3.70d94-350[»]
5VHFelectron microscopy5.70d215-350[»]
5VHHelectron microscopy6.10d94-350[»]
5VHIelectron microscopy6.80d94-350[»]
5VHSelectron microscopy8.80d94-350[»]
ProteinModelPortaliP48556.
SMRiP48556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 331PCIPROSITE-ProRule annotationAdd BLAST170

Sequence similaritiesi

Belongs to the proteasome subunit S14 family.Curated

Phylogenomic databases

eggNOGiKOG3151. Eukaryota.
ENOG410XXP2. LUCA.
GeneTreeiENSGT00390000014682.
HOGENOMiHOG000196008.
HOVERGENiHBG024389.
InParanoidiP48556.
KOiK03031.
OMAiGAFWSIR.
OrthoDBiEOG091G0G73.
PhylomeDBiP48556.
TreeFamiTF106233.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR006746. 26S_Psome_Rpn12.
IPR033464. CSN8_PSD8_EIF3K.
IPR036388. WH-like_DNA-bd_sf.
PANTHERiPTHR12387. PTHR12387. 1 hit.
PfamiView protein in Pfam
PF10075. CSN8_PSD8_EIF3K. 1 hit.
PROSITEiView protein in PROSITE
PS50250. PCI. 1 hit.

Sequencei

Sequence statusi: Complete.

P48556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIKGRAPRA PPRERRRATR GGLRQVVAPP RALGSTSRPH FRRASVCRRR
60 70 80 90 100
CRKSGGLLAA SRKMAAAAVN GAAGFSSSGP AATSGAVLQA ATGMYEQLKG
110 120 130 140 150
EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI
160 170 180 190 200
GAQWSILRKD IPSFERYMAQ LKCYYFDYKE QLPESAYMHQ LLGLNLLFLL
210 220 230 240 250
SQNRVAEFHT ELERLPAKDI QTNVYIKHPV SLEQYLMEGS YNKVFLAKGN
260 270 280 290 300
IPAESYTFFI DILLDTIRDE IAGCIEKAYE KILFTEATRI LFFNTPKKMT
310 320 330 340 350
DYAKKRGWVL GPNNYYSFAS QQQKPEDTTI PSTELAKQVI EYARQLEMIV
Length:350
Mass (Da):39,612
Last modified:October 5, 2010 - v2
Checksum:iFF20203213333CB3
GO

Sequence cautioni

The sequence AAC62833 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAH01164 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH65006 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA07237 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK301771 mRNA. Translation: BAG63230.1.
AC005789 Genomic DNA. Translation: AAC62833.1. Sequence problems.
CH471126 Genomic DNA. Translation: EAW56785.1.
BC001164 mRNA. Translation: AAH01164.3. Different initiation.
BC065006 mRNA. Translation: AAH65006.2. Different initiation.
D38047 mRNA. Translation: BAA07237.1. Different initiation.
CCDSiCCDS12515.2.
PIRiS56108.
RefSeqiNP_002803.2. NM_002812.4.
UniGeneiHs.78466.

Genome annotation databases

EnsembliENST00000215071; ENSP00000215071; ENSG00000099341.
ENST00000620216; ENSP00000481136; ENSG00000099341.
GeneIDi5714.
KEGGihsa:5714.

Similar proteinsi

Entry informationi

Entry nameiPSMD8_HUMAN
AccessioniPrimary (citable) accession number: P48556
Secondary accession number(s): B4DX18, Q6P1L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 5, 2010
Last modified: November 22, 2017
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-64 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families