Reviewed, UniProtKB/Swiss-Prot P48556 (PSMD8_HUMAN)

Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 8
Alternative name(s):
    26S proteasome regulatory subunit S14
    p31

Gene names

Name:

PSMD8

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Necessary for activation of the CDC28 kinase.
Sequence similarities	Belongs to the proteasome subunit S14 family.

Ontologies

Keywords
Cellular component	Proteasome
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: HPA proteasome regulatory particle Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAAF1	Q9BRP4	1	EBI-359304,EBI-1056358
PSMD6	Q15008	1	EBI-359304,EBI-359701

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	26S proteasome non-ATPase regulatory subunit 8		PRO_0000173846

Sequences

	Sequence		Length	Mass (Da)
	P48556-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: A0C7FFA8C625FC17	FASTA	257	30,005
	10 20 30 40 50 60 MYEQLKGEWN RKSPNLSKCG EELGRLKLVL LELNFLPTTG TKLTKQQLIL ARDILEIGAQ 70 80 90 100 110 120 WSILRKDIPS FERYMAQLKC YYFDYKEQLP ESAYMHQLLG LNLLFLLSQN RVAEFHTELE 130 140 150 160 170 180 RLPAKDIQTN VYIKHPVSLE QYLMEGSYNK VFLAKGNIPA ESYTFFIDIL LDTIRDEIAG 190 200 210 220 230 240 CIEKAYEKIL FTEATRILFF NTPKKMTDYA KKRGWVLGPN NYYSFASQQQ KPEDTTIPST 250 ELAKQVIEYA RQLEMIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nin1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae." Kominami K., DeMartino G.N., Moomaw C., Slaughter C.A., Shimbara N., Fujimuro M., Yokosawa H., Hisamatsu H., Tanahashi N., Shimizu Y., Tanaka K., Toh-e A. EMBO J. 14:3105-3115(1995) [PubMed: 7621825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle and Skin.
[4]	"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D38047 mRNA. Translation: BAA07237.1. AC005789 Genomic DNA. Translation: AAC62833.1. BC001164 mRNA. Translation: AAH01164.3. BC065006 mRNA. Translation: AAH65006.2.
IPI	IPI00010201.
PIR	S56108.
RefSeq	NP_002803.2.
UniGene	Hs.78466
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P48556. 6 interactions.
Proteomic databases
PRIDE	P48556.
Genome annotation databases
Ensembl	ENSG00000099341. Homo sapiens. [Contig view]
GeneID	5714.
KEGG	hsa:5714.
Organism-specific databases
GeneCards	GC19P043560.
H-InvDB	HIX0040074.
HGNC	HGNC:9566. PSMD8.
HPA	HPA006702.
PharmGKB	PA33912.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P48556.
Enzyme and pathway databases
Reactome	REACT_11045. Signaling by Wnt. REACT_13. Metabolism of amino acids. REACT_13635. Regulation of activated PAK-2p34 by proteasome mediated degradation. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.
Gene expression databases
ArrayExpress	P48556.
Bgee	P48556.
CleanEx	HS_PSMD8.
GermOnline	ENSG00000099341. Homo sapiens.
Family and domain databases
InterPro	IPR006746. 26S_Psome_Rpn12. IPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25. [Graphical view]
PANTHER	PTHR12387. Nin1_C. 1 hit.
Pfam	PF03399. SAC3_GANP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	22198.

Entry information

Entry name

PSMD8_HUMAN

Accession

Primary (citable) accession number: P48556
Secondary accession number(s): Q6P1L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	July 7, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)