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P48550

- KCNJ6_RAT

UniProt

P48550 - KCNJ6_RAT

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Protein

G protein-activated inward rectifier potassium channel 2

Gene

Kcnj6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This potassium channel is controlled by G proteins. It may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium or cesium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei184 – 1841Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. G-protein activated inward rectifier potassium channel activity Source: InterPro
  2. G-protein alpha-subunit binding Source: RGD

GO - Biological processi

  1. cellular response to morphine Source: RGD
  2. response to electrical stimulus Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 2
Short name:
GIRK-2
Alternative name(s):
BIR1
Inward rectifier K(+) channel Kir3.2
KATP-2
Potassium channel, inwardly rectifying subfamily J member 6
Gene namesi
Name:Kcnj6
Synonyms:Girk2, Kcnj7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2959. Kcnj6.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: RGD
  2. cell surface Source: RGD
  3. cytoplasm Source: RGD
  4. dendrite Source: RGD
  5. integral component of membrane Source: UniProtKB-KW
  6. neuronal cell body membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425G protein-activated inward rectifier potassium channel 2PRO_0000154946Add
BLAST

PTM databases

PhosphoSiteiP48550.

Expressioni

Tissue specificityi

Pancreatic beta cells and brain.

Gene expression databases

GenevestigatoriP48550.

Interactioni

Subunit structurei

Associates with GIRK1 or GIRK4 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity). Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliP48550.
SMRiP48550. Positions 202-380.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9191CytoplasmicBy similarityAdd
BLAST
Topological domaini117 – 14024ExtracellularBy similarityAdd
BLAST
Topological domaini160 – 1689ExtracellularBy similarity
Topological domaini191 – 425235CytoplasmicBy similarityAdd
BLAST

Intramembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei141 – 15212Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei153 – 1597Pore-formingBy similarity

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei92 – 11625Helical; Name=M1By similarityAdd
BLAST
Transmembranei169 – 19022Helical; Name=M2By similarityAdd
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi154 – 1596Selectivity filterBy similarity
Motifi422 – 4254PDZ-binding

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48550.
KOiK05000.
PhylomeDBiP48550.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003275. K_chnl_inward-rec_Kir3.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF19. PTHR11767:SF19. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01328. KIR32CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P48550-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR
60 70 80 90 100
TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDLK WRFNLLIFVM
110 120 130 140 150
VYTVTWLFFG MIWWLIAYIR GDMDHIEDPS WTPCVTNLNG FVSAFLFSIE
160 170 180 190 200
TETTIGYGYR VITDKCPEGI ILLLIQSVLG SIVNAFMVGC MFVKISQPKK
210 220 230 240 250
RAETLVFSTH AVISMRDGKL CLMFRVGDLR NSHIVEASIR AKLIKSKQTS
260 270 280 290 300
EGEFIPLNQT DINVGYYTGD DRLFLVSPLI ISHEINQQSP FWEISKAQLP
310 320 330 340 350
KEELEIVVIL EGMVEATGMT CQARSSYVTS EILWGYRFTP VLTLEDGFYE
360 370 380 390 400
VDYNSFHETH ETSTPSLSAK ELAELANRAE LPLSWSVSSK LNQHAELETE
410 420
EEEKNPEELT ERNGDVANLE NESKV
Length:425
Mass (Da):48,640
Last modified:February 1, 1996 - v1
Checksum:i66CE9599A4AE38D3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti328 – 3281V → I in AAA87002. (PubMed:7626127)Curated
Sequence conflicti360 – 3601H → Y in AAA87002. (PubMed:7626127)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83583 mRNA. Translation: CAA58566.1.
U21087 mRNA. Translation: AAA87002.1.
PIRiS66266. S52852.
RefSeqiNP_037324.1. NM_013192.2.
UniGeneiRn.10185.

Genome annotation databases

GeneIDi25743.
KEGGirno:25743.
UCSCiRGD:2959. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83583 mRNA. Translation: CAA58566.1 .
U21087 mRNA. Translation: AAA87002.1 .
PIRi S66266. S52852.
RefSeqi NP_037324.1. NM_013192.2.
UniGenei Rn.10185.

3D structure databases

ProteinModelPortali P48550.
SMRi P48550. Positions 202-380.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P48550.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25743.
KEGGi rno:25743.
UCSCi RGD:2959. rat.

Organism-specific databases

CTDi 3763.
RGDi 2959. Kcnj6.

Phylogenomic databases

eggNOGi NOG72812.
HOGENOMi HOG000237325.
HOVERGENi HBG006178.
InParanoidi P48550.
KOi K05000.
PhylomeDBi P48550.

Miscellaneous databases

NextBioi 607907.
PROi P48550.

Gene expression databases

Genevestigatori P48550.

Family and domain databases

Gene3Di 2.60.40.1400. 1 hit.
InterProi IPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003275. K_chnl_inward-rec_Kir3.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view ]
PANTHERi PTHR11767. PTHR11767. 1 hit.
PTHR11767:SF19. PTHR11767:SF19. 1 hit.
Pfami PF01007. IRK. 1 hit.
[Graphical view ]
PIRSFi PIRSF005465. GIRK_kir. 1 hit.
PRINTSi PR01328. KIR32CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMi SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain."
    Bond C.T., Aemmaelae C., Ashfield R., Blair T.A., Gribble F., Khan R.N., Lee K., Proks P., Rowe I.C.M., Sakura H., Ashford M.J., Adelman J.P., Ashcroft F.M.
    FEBS Lett. 367:61-66(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Cloning of rat KATP-2 channel and decreased expression in pancreatic islets of male Zucker diabetic fatty rats."
    Stoffel M., Tokuyama Y., Trabb J.B., German M.S., Tsaar M.L., Jan L.Y., Polonsky K.S., Bell G.I.
    Biochem. Biophys. Res. Commun. 212:894-899(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "A unique sorting nexin regulates trafficking of potassium channels via a PDZ domain interaction."
    Lunn M.L., Nassirpour R., Arrabit C., Tan J., McLeod I., Arias C.M., Sawchenko P.E., Yates J.R. III, Slesinger P.A.
    Nat. Neurosci. 10:1249-1259(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNX27.

Entry informationi

Entry nameiKCNJ6_RAT
AccessioniPrimary (citable) accession number: P48550
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3