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Protein

G protein-activated inward rectifier potassium channel 2

Gene

Kcnj6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This potassium channel is controlled by G proteins. It may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium or cesium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei184Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to morphine Source: RGD
  • potassium ion import Source: GO_Central
  • response to electrical stimulus Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 2
Short name:
GIRK-2
Alternative name(s):
BIR1
Inward rectifier K(+) channel Kir3.2
KATP-2
Potassium channel, inwardly rectifying subfamily J member 6
Gene namesi
Name:Kcnj6
Synonyms:Girk2, Kcnj7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2959. Kcnj6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 91CytoplasmicBy similarityAdd BLAST91
Transmembranei92 – 116Helical; Name=M1By similarityAdd BLAST25
Topological domaini117 – 140ExtracellularBy similarityAdd BLAST24
Intramembranei141 – 152Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei153 – 159Pore-formingBy similarity7
Topological domaini160 – 168ExtracellularBy similarity9
Transmembranei169 – 190Helical; Name=M2By similarityAdd BLAST22
Topological domaini191 – 425CytoplasmicBy similarityAdd BLAST235

GO - Cellular componenti

  • axon Source: RGD
  • cell surface Source: RGD
  • cytoplasm Source: RGD
  • dendrite Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • neuronal cell body membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549461 – 425G protein-activated inward rectifier potassium channel 2Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei25PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP48550.
PRIDEiP48550.

PTM databases

iPTMnetiP48550.
PhosphoSitePlusiP48550.

Expressioni

Tissue specificityi

Pancreatic beta cells and brain.

Interactioni

Subunit structurei

Associates with GIRK1 or GIRK4 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity). Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes.By similarity1 Publication

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059308.

Structurei

3D structure databases

ProteinModelPortaliP48550.
SMRiP48550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi154 – 159Selectivity filterBy similarity6
Motifi422 – 425PDZ-binding4

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48550.
KOiK05000.
PhylomeDBiP48550.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003275. K_chnl_inward-rec_Kir3.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF19. PTHR11767:SF19. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01328. KIR32CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P48550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR
60 70 80 90 100
TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDLK WRFNLLIFVM
110 120 130 140 150
VYTVTWLFFG MIWWLIAYIR GDMDHIEDPS WTPCVTNLNG FVSAFLFSIE
160 170 180 190 200
TETTIGYGYR VITDKCPEGI ILLLIQSVLG SIVNAFMVGC MFVKISQPKK
210 220 230 240 250
RAETLVFSTH AVISMRDGKL CLMFRVGDLR NSHIVEASIR AKLIKSKQTS
260 270 280 290 300
EGEFIPLNQT DINVGYYTGD DRLFLVSPLI ISHEINQQSP FWEISKAQLP
310 320 330 340 350
KEELEIVVIL EGMVEATGMT CQARSSYVTS EILWGYRFTP VLTLEDGFYE
360 370 380 390 400
VDYNSFHETH ETSTPSLSAK ELAELANRAE LPLSWSVSSK LNQHAELETE
410 420
EEEKNPEELT ERNGDVANLE NESKV
Length:425
Mass (Da):48,640
Last modified:February 1, 1996 - v1
Checksum:i66CE9599A4AE38D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti328V → I in AAA87002 (PubMed:7626127).Curated1
Sequence conflicti360H → Y in AAA87002 (PubMed:7626127).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83583 mRNA. Translation: CAA58566.1.
U21087 mRNA. Translation: AAA87002.1.
PIRiS66266. S52852.
RefSeqiNP_037324.1. NM_013192.2.
UniGeneiRn.10185.

Genome annotation databases

GeneIDi25743.
KEGGirno:25743.
UCSCiRGD:2959. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83583 mRNA. Translation: CAA58566.1.
U21087 mRNA. Translation: AAA87002.1.
PIRiS66266. S52852.
RefSeqiNP_037324.1. NM_013192.2.
UniGeneiRn.10185.

3D structure databases

ProteinModelPortaliP48550.
SMRiP48550.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059308.

PTM databases

iPTMnetiP48550.
PhosphoSitePlusiP48550.

Proteomic databases

PaxDbiP48550.
PRIDEiP48550.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25743.
KEGGirno:25743.
UCSCiRGD:2959. rat.

Organism-specific databases

CTDi3763.
RGDi2959. Kcnj6.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48550.
KOiK05000.
PhylomeDBiP48550.

Miscellaneous databases

PROiP48550.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003275. K_chnl_inward-rec_Kir3.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF19. PTHR11767:SF19. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01328. KIR32CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNJ6_RAT
AccessioniPrimary (citable) accession number: P48550
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.