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P48550 (IRK6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-activated inward rectifier potassium channel 2

Short name=GIRK-2
Alternative name(s):
BIR1
Inward rectifier K(+) channel Kir3.2
KATP-2
Potassium channel, inwardly rectifying subfamily J member 6
Gene names
Name:Kcnj6
Synonyms:Girk2, Kcnj7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This potassium channel is controlled by G proteins. It may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium or cesium.

Subunit structure

Associates with GIRK1 or GIRK4 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Pancreatic beta cells and brain.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425G protein-activated inward rectifier potassium channel 2
PRO_0000154946

Regions

Topological domain1 – 9191Cytoplasmic By similarity
Transmembrane92 – 11625Helical; Name=M1; By similarity
Topological domain117 – 14024Extracellular By similarity
Intramembrane141 – 15212Helical; Pore-forming; Name=H5; By similarity
Intramembrane153 – 1597Pore-forming; By similarity
Topological domain160 – 1689Extracellular By similarity
Transmembrane169 – 19022Helical; Name=M2; By similarity
Topological domain191 – 425235Cytoplasmic By similarity
Motif154 – 1596Selectivity filter By similarity
Motif422 – 4254PDZ-binding

Sites

Site1841Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Experimental info

Sequence conflict3281V → I in AAA87002. Ref.2
Sequence conflict3601H → Y in AAA87002. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P48550 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 66CE9599A4AE38D3

FASTA42548,640
        10         20         30         40         50         60 
MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR TKRKIQRYVR 

        70         80         90        100        110        120 
KDGKCNVHHG NVRETYRYLT DIFTTLVDLK WRFNLLIFVM VYTVTWLFFG MIWWLIAYIR 

       130        140        150        160        170        180 
GDMDHIEDPS WTPCVTNLNG FVSAFLFSIE TETTIGYGYR VITDKCPEGI ILLLIQSVLG 

       190        200        210        220        230        240 
SIVNAFMVGC MFVKISQPKK RAETLVFSTH AVISMRDGKL CLMFRVGDLR NSHIVEASIR 

       250        260        270        280        290        300 
AKLIKSKQTS EGEFIPLNQT DINVGYYTGD DRLFLVSPLI ISHEINQQSP FWEISKAQLP 

       310        320        330        340        350        360 
KEELEIVVIL EGMVEATGMT CQARSSYVTS EILWGYRFTP VLTLEDGFYE VDYNSFHETH 

       370        380        390        400        410        420 
ETSTPSLSAK ELAELANRAE LPLSWSVSSK LNQHAELETE EEEKNPEELT ERNGDVANLE 


NESKV 

« Hide

References

[1]"Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain."
Bond C.T., Aemmaelae C., Ashfield R., Blair T.A., Gribble F., Khan R.N., Lee K., Proks P., Rowe I.C.M., Sakura H., Ashford M.J., Adelman J.P., Ashcroft F.M.
FEBS Lett. 367:61-66(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Cloning of rat KATP-2 channel and decreased expression in pancreatic islets of male Zucker diabetic fatty rats."
Stoffel M., Tokuyama Y., Trabb J.B., German M.S., Tsaar M.L., Jan L.Y., Polonsky K.S., Bell G.I.
Biochem. Biophys. Res. Commun. 212:894-899(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"A unique sorting nexin regulates trafficking of potassium channels via a PDZ domain interaction."
Lunn M.L., Nassirpour R., Arrabit C., Tan J., McLeod I., Arias C.M., Sawchenko P.E., Yates J.R. III, Slesinger P.A.
Nat. Neurosci. 10:1249-1259(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNX27.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83583 mRNA. Translation: CAA58566.1.
U21087 mRNA. Translation: AAA87002.1.
PIRS52852. S66266.
RefSeqNP_037324.1. NM_013192.2.
UniGeneRn.10185.

3D structure databases

ProteinModelPortalP48550.
SMRP48550. Positions 202-380.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP48550.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25743.
KEGGrno:25743.
UCSCRGD:2959. rat.

Organism-specific databases

CTD3763.
RGD2959. Kcnj6.

Phylogenomic databases

eggNOGNOG72812.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP48550.
KOK05000.
PhylomeDBP48550.

Gene expression databases

GenevestigatorP48550.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003275. K_chnl_inward-rec_Kir3.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF19. PTHR11767:SF19. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01328. KIR32CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio607907.
PROP48550.

Entry information

Entry nameIRK6_RAT
AccessionPrimary (citable) accession number: P48550
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families