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P48550

- KCNJ6_RAT

UniProt

P48550 - KCNJ6_RAT

Protein

G protein-activated inward rectifier potassium channel 2

Gene

Kcnj6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    This potassium channel is controlled by G proteins. It may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium or cesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei184 – 1841Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. G-protein activated inward rectifier potassium channel activity Source: InterPro
    2. G-protein alpha-subunit binding Source: RGD
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to morphine Source: RGD
    2. response to electrical stimulus Source: RGD

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-activated inward rectifier potassium channel 2
    Short name:
    GIRK-2
    Alternative name(s):
    BIR1
    Inward rectifier K(+) channel Kir3.2
    KATP-2
    Potassium channel, inwardly rectifying subfamily J member 6
    Gene namesi
    Name:Kcnj6
    Synonyms:Girk2, Kcnj7
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2959. Kcnj6.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: RGD
    2. cell surface Source: RGD
    3. cytoplasm Source: RGD
    4. dendrite Source: RGD
    5. integral component of membrane Source: UniProtKB-KW
    6. neuronal cell body membrane Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 425425G protein-activated inward rectifier potassium channel 2PRO_0000154946Add
    BLAST

    PTM databases

    PhosphoSiteiP48550.

    Expressioni

    Tissue specificityi

    Pancreatic beta cells and brain.

    Gene expression databases

    GenevestigatoriP48550.

    Interactioni

    Subunit structurei

    Associates with GIRK1 or GIRK4 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes.By similarity1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP48550.
    SMRiP48550. Positions 202-380.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9191CytoplasmicBy similarityAdd
    BLAST
    Topological domaini117 – 14024ExtracellularBy similarityAdd
    BLAST
    Topological domaini160 – 1689ExtracellularBy similarity
    Topological domaini191 – 425235CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei141 – 15212Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei153 – 1597Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei92 – 11625Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei169 – 19022Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi154 – 1596Selectivity filterBy similarity
    Motifi422 – 4254PDZ-binding

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiP48550.
    KOiK05000.
    PhylomeDBiP48550.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003275. K_chnl_inward-rec_Kir3.2.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF19. PTHR11767:SF19. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01328. KIR32CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P48550-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR    50
    TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDLK WRFNLLIFVM 100
    VYTVTWLFFG MIWWLIAYIR GDMDHIEDPS WTPCVTNLNG FVSAFLFSIE 150
    TETTIGYGYR VITDKCPEGI ILLLIQSVLG SIVNAFMVGC MFVKISQPKK 200
    RAETLVFSTH AVISMRDGKL CLMFRVGDLR NSHIVEASIR AKLIKSKQTS 250
    EGEFIPLNQT DINVGYYTGD DRLFLVSPLI ISHEINQQSP FWEISKAQLP 300
    KEELEIVVIL EGMVEATGMT CQARSSYVTS EILWGYRFTP VLTLEDGFYE 350
    VDYNSFHETH ETSTPSLSAK ELAELANRAE LPLSWSVSSK LNQHAELETE 400
    EEEKNPEELT ERNGDVANLE NESKV 425
    Length:425
    Mass (Da):48,640
    Last modified:February 1, 1996 - v1
    Checksum:i66CE9599A4AE38D3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti328 – 3281V → I in AAA87002. (PubMed:7626127)Curated
    Sequence conflicti360 – 3601H → Y in AAA87002. (PubMed:7626127)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83583 mRNA. Translation: CAA58566.1.
    U21087 mRNA. Translation: AAA87002.1.
    PIRiS66266. S52852.
    RefSeqiNP_037324.1. NM_013192.2.
    UniGeneiRn.10185.

    Genome annotation databases

    GeneIDi25743.
    KEGGirno:25743.
    UCSCiRGD:2959. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83583 mRNA. Translation: CAA58566.1 .
    U21087 mRNA. Translation: AAA87002.1 .
    PIRi S66266. S52852.
    RefSeqi NP_037324.1. NM_013192.2.
    UniGenei Rn.10185.

    3D structure databases

    ProteinModelPortali P48550.
    SMRi P48550. Positions 202-380.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P48550.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25743.
    KEGGi rno:25743.
    UCSCi RGD:2959. rat.

    Organism-specific databases

    CTDi 3763.
    RGDi 2959. Kcnj6.

    Phylogenomic databases

    eggNOGi NOG72812.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi P48550.
    KOi K05000.
    PhylomeDBi P48550.

    Miscellaneous databases

    NextBioi 607907.
    PROi P48550.

    Gene expression databases

    Genevestigatori P48550.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003275. K_chnl_inward-rec_Kir3.2.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF19. PTHR11767:SF19. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01328. KIR32CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional expression of the cDNA encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain."
      Bond C.T., Aemmaelae C., Ashfield R., Blair T.A., Gribble F., Khan R.N., Lee K., Proks P., Rowe I.C.M., Sakura H., Ashford M.J., Adelman J.P., Ashcroft F.M.
      FEBS Lett. 367:61-66(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "Cloning of rat KATP-2 channel and decreased expression in pancreatic islets of male Zucker diabetic fatty rats."
      Stoffel M., Tokuyama Y., Trabb J.B., German M.S., Tsaar M.L., Jan L.Y., Polonsky K.S., Bell G.I.
      Biochem. Biophys. Res. Commun. 212:894-899(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "A unique sorting nexin regulates trafficking of potassium channels via a PDZ domain interaction."
      Lunn M.L., Nassirpour R., Arrabit C., Tan J., McLeod I., Arias C.M., Sawchenko P.E., Yates J.R. III, Slesinger P.A.
      Nat. Neurosci. 10:1249-1259(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNX27.

    Entry informationi

    Entry nameiKCNJ6_RAT
    AccessioniPrimary (citable) accession number: P48550
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3