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Protein

G protein-activated inward rectifier potassium channel 4

Gene

Kcnj5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1791Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. G-protein activated inward rectifier potassium channel activity Source: GO_Central

GO - Biological processi

  1. potassium ion import Source: GO_Central
  2. regulation of ion transmembrane transport Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 4
Short name:
GIRK-4
Alternative name(s):
Cardiac inward rectifier
Short name:
CIR
Heart KATP channel
Inward rectifier K(+) channel Kir3.4
KATP-1
Potassium channel, inwardly rectifying subfamily J member 5
Gene namesi
Name:Kcnj5
Synonyms:Girk4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61971. Kcnj5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8686CytoplasmicBy similarityAdd
BLAST
Transmembranei87 – 11125Helical; Name=M1By similarityAdd
BLAST
Topological domaini112 – 13524ExtracellularBy similarityAdd
BLAST
Intramembranei136 – 14712Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei148 – 1547Pore-formingBy similarity
Topological domaini155 – 1639ExtracellularBy similarity
Transmembranei164 – 18522Helical; Name=M2By similarityAdd
BLAST
Topological domaini186 – 419234CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: RGD
  2. integral component of plasma membrane Source: GO_Central
  3. T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419G protein-activated inward rectifier potassium channel 4PRO_0000154937Add
BLAST

Proteomic databases

PRIDEiP48548.

PTM databases

PhosphoSiteiP48548.

Expressioni

Tissue specificityi

Most abundant in heart tissue where it is found predominantly in atria. Also found in brain, kidney, liver, spleen, lung and thymus.

Gene expression databases

GenevestigatoriP48548.

Interactioni

Subunit structurei

Associates with GIRK1 or GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048134.

Structurei

3D structure databases

ProteinModelPortaliP48548.
SMRiP48548. Positions 197-375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi149 – 1546Selectivity filterBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi402 – 4054Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48548.
KOiK04999.
PhylomeDBiP48548.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003277. K_chnl_inward-rec_Kir3.4.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01330. KIR34CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P48548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDSRNAMN QDMEIGVTSQ DHKKIPKQAR DYIPIATDRT RLLPEGKKPR
60 70 80 90 100
QRYMEKTGKC NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTIT
110 120 130 140 150
WLFFGFIWWL IAYVRGDLDH VGDQEWIPCV ENLSGFVSAF LFSIETETTI
160 170 180 190 200
GYGFRVITEK CPEGIILLLV QAILGSIVNA FMVGCMFVKI SQPKKRAETL
210 220 230 240 250
MFSNNAVISM RDEKLCLMFR VGDLRNSHIV EASIRAKLIK SRQTKEGEFI
260 270 280 290 300
PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLEQEEFE
310 320 330 340 350
VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT
360 370 380 390 400
FHDTYETNTP SCCAKELAEM KRNGQLLQSL PSPPLLGGCA EAEKEAEAEH
410
DEEEEPNGLS VSRATRGSM
Length:419
Mass (Da):47,783
Last modified:November 1, 1997 - v2
Checksum:iDFF537CD79A7370D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881V → I in CAA58567 (PubMed:8047164).Curated
Sequence conflicti375 – 3751Q → E in CAA58567 (PubMed:8047164).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83584 mRNA. Translation: CAA58567.1.
L35771 mRNA. Translation: AAC42048.1.
D50135 mRNA. Translation: BAA08815.1.
BC087022 mRNA. Translation: AAH87022.1.
PIRiS48077.
RefSeqiNP_058993.1. NM_017297.2.
UniGeneiRn.10047.

Genome annotation databases

GeneIDi29713.
KEGGirno:29713.
UCSCiRGD:61971. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83584 mRNA. Translation: CAA58567.1.
L35771 mRNA. Translation: AAC42048.1.
D50135 mRNA. Translation: BAA08815.1.
BC087022 mRNA. Translation: AAH87022.1.
PIRiS48077.
RefSeqiNP_058993.1. NM_017297.2.
UniGeneiRn.10047.

3D structure databases

ProteinModelPortaliP48548.
SMRiP48548. Positions 197-375.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048134.

Chemistry

GuidetoPHARMACOLOGYi437.

PTM databases

PhosphoSiteiP48548.

Proteomic databases

PRIDEiP48548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29713.
KEGGirno:29713.
UCSCiRGD:61971. rat.

Organism-specific databases

CTDi3762.
RGDi61971. Kcnj5.

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48548.
KOiK04999.
PhylomeDBiP48548.

Miscellaneous databases

NextBioi610153.
PROiP48548.

Gene expression databases

GenevestigatoriP48548.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003277. K_chnl_inward-rec_Kir3.4.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01330. KIR34CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional expression of a rat heart KATP channel."
    Ashford M.L.J., Bond C.T., Blair T.A., Adelman J.P.
    Nature 370:456-459(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart.
  2. "The G-protein-gated atrial K+ channel IKACh is a heteromultimer of two inwardly rectifying K(+)-channel proteins."
    Krapivinsky G.B., Gordon E.A., Wickman K., Velimirovic B., Krapivinsky L.D., Clapham D.E.
    Nature 374:135-141(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 31-39 AND 333-341.
    Strain: Sprague-Dawley.
    Tissue: Neonatal heart atrium.
  3. "Pancreatic islet cells express a family of inwardly rectifying K+ channel subunits which interact to form G-protein-activated channels."
    Ferrer J., Nichols C.G., Makhina E.N., Salkoff L., Bernstein J., Gerhard D., Wasson J., Ramanadham S., Permutt A.
    J. Biol. Chem. 270:26086-26091(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreatic islet.
  4. "Functional characterization and localization of a cardiac-type inwardly rectifying K+ channel."
    Iizuka M., Kubo Y., Tsunenari I., Pan C.X., Akiba I., Kono T.
    Recept. Channels 3:299-315(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiKCNJ5_RAT
AccessioniPrimary (citable) accession number: P48548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: February 4, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.