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P48548 (KCNJ5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-activated inward rectifier potassium channel 4

Short name=GIRK-4
Alternative name(s):
Cardiac inward rectifier
Short name=CIR
Heart KATP channel
Inward rectifier K(+) channel Kir3.4
KATP-1
Potassium channel, inwardly rectifying subfamily J member 5
Gene names
Name:Kcnj5
Synonyms:Girk4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

Subunit structure

Associates with GIRK1 or GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Most abundant in heart tissue where it is found predominantly in atria. Also found in brain, kidney, liver, spleen, lung and thymus.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419G protein-activated inward rectifier potassium channel 4
PRO_0000154937

Regions

Topological domain1 – 8686Cytoplasmic By similarity
Transmembrane87 – 11125Helical; Name=M1; By similarity
Topological domain112 – 13524Extracellular By similarity
Intramembrane136 – 14712Helical; Pore-forming; Name=H5; By similarity
Intramembrane148 – 1547Pore-forming; By similarity
Topological domain155 – 1639Extracellular By similarity
Transmembrane164 – 18522Helical; Name=M2; By similarity
Topological domain186 – 419234Cytoplasmic By similarity
Motif149 – 1546Selectivity filter By similarity
Compositional bias402 – 4054Poly-Glu

Sites

Site1791Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Experimental info

Sequence conflict1881V → I in CAA58567. Ref.1
Sequence conflict3751Q → E in CAA58567. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48548 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: DFF537CD79A7370D

FASTA41947,783
        10         20         30         40         50         60 
MAGDSRNAMN QDMEIGVTSQ DHKKIPKQAR DYIPIATDRT RLLPEGKKPR QRYMEKTGKC 

        70         80         90        100        110        120 
NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTIT WLFFGFIWWL IAYVRGDLDH 

       130        140        150        160        170        180 
VGDQEWIPCV ENLSGFVSAF LFSIETETTI GYGFRVITEK CPEGIILLLV QAILGSIVNA 

       190        200        210        220        230        240 
FMVGCMFVKI SQPKKRAETL MFSNNAVISM RDEKLCLMFR VGDLRNSHIV EASIRAKLIK 

       250        260        270        280        290        300 
SRQTKEGEFI PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLEQEEFE 

       310        320        330        340        350        360 
VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT FHDTYETNTP 

       370        380        390        400        410 
SCCAKELAEM KRNGQLLQSL PSPPLLGGCA EAEKEAEAEH DEEEEPNGLS VSRATRGSM 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and functional expression of a rat heart KATP channel."
Ashford M.L.J., Bond C.T., Blair T.A., Adelman J.P.
Nature 370:456-459(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Heart.
[2]Erratum
Ashford M.L.J., Bond C.T., Blair T.A., Adelman J.P.
Nature 378:792-792(1995) [PubMed] [Europe PMC] [Abstract]
[3]"The G-protein-gated atrial K+ channel IKACh is a heteromultimer of two inwardly rectifying K(+)-channel proteins."
Krapivinsky G.B., Gordon E.A., Wickman K., Velimirovic B., Krapivinsky L.D., Clapham D.E.
Nature 374:135-141(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 31-39 AND 333-341.
Strain: Sprague-Dawley.
Tissue: Neonatal heart atrium.
[4]"Pancreatic islet cells express a family of inwardly rectifying K+ channel subunits which interact to form G-protein-activated channels."
Ferrer J., Nichols C.G., Makhina E.N., Salkoff L., Bernstein J., Gerhard D., Wasson J., Ramanadham S., Permutt A.
J. Biol. Chem. 270:26086-26091(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pancreatic islet.
[5]"Functional characterization and localization of a cardiac-type inwardly rectifying K+ channel."
Iizuka M., Kubo Y., Tsunenari I., Pan C.X., Akiba I., Kono T.
Recept. Channels 3:299-315(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Heart.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83584 mRNA. Translation: CAA58567.1.
L35771 mRNA. Translation: AAC42048.1.
D50135 mRNA. Translation: BAA08815.1.
BC087022 mRNA. Translation: AAH87022.1.
PIRS48077.
RefSeqNP_058993.1. NM_017297.2.
UniGeneRn.10047.

3D structure databases

ProteinModelPortalP48548.
SMRP48548. Positions 197-375.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000048134.

Chemistry

GuidetoPHARMACOLOGY437.

PTM databases

PhosphoSiteP48548.

Proteomic databases

PRIDEP48548.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29713.
KEGGrno:29713.
UCSCRGD:61971. rat.

Organism-specific databases

CTD3762.
RGD61971. Kcnj5.

Phylogenomic databases

eggNOGNOG72812.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP48548.
KOK04999.
PhylomeDBP48548.

Gene expression databases

GenevestigatorP48548.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003277. K_chnl_inward-rec_Kir3.4.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01330. KIR34CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio610153.
PROP48548.

Entry information

Entry nameKCNJ5_RAT
AccessionPrimary (citable) accession number: P48548
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families