G protein-activated inward rectifier potassium channel 4

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P48548 (IRK5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
G protein-activated inward rectifier potassium channel 4
Short name=GIRK-4

Alternative name(s):
Cardiac inward rectifier
Short name=CIR
Heart KATP channel
Inward rectifier K(+) channel Kir3.4
KATP-1
Potassium channel, inwardly rectifying subfamily J member 5

Gene names

Name:	Kcnj5
Synonyms:	Girk4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
Subunit structure	Associates with GIRK1 or GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Most abundant in heart tissue where it is found predominantly in atria. Also found in brain, kidney, liver, spleen, lung and thymus.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Voltage-gated channel
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	T-tubule Inferred from direct assay PubMed 11561006. Source: RGD external side of plasma membrane Inferred from direct assay PubMed 11561006. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein activated inward rectifier potassium channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 419

419

G protein-activated inward rectifier potassium channel 4

PRO_0000154937

Regions

Topological domain

1 – 86

Cytoplasmic By similarity

Transmembrane

87 – 111

Helical; Name=M1; By similarity

Topological domain

112 – 135

Extracellular By similarity

Intramembrane

136 – 147

Helical; Pore-forming; Name=H5; By similarity

Intramembrane

148 – 154

Pore-forming; By similarity

Topological domain

155 – 163

Extracellular By similarity

Transmembrane

164 – 185

Helical; Name=M2; By similarity

Topological domain

186 – 419

234

Cytoplasmic By similarity

Motif

149 – 154

Selectivity filter By similarity

Compositional bias

402 – 405

Poly-Glu

Sites

Site

179

Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Experimental info

Sequence conflict

188

V → I in CAA58567. Ref.1

Sequence conflict

375

Q → E in CAA58567. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P48548 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: DFF537CD79A7370D
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FASTA

419

47,783

        10         20         30         40         50         60 
MAGDSRNAMN QDMEIGVTSQ DHKKIPKQAR DYIPIATDRT RLLPEGKKPR QRYMEKTGKC 

        70         80         90        100        110        120 
NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTIT WLFFGFIWWL IAYVRGDLDH 

       130        140        150        160        170        180 
VGDQEWIPCV ENLSGFVSAF LFSIETETTI GYGFRVITEK CPEGIILLLV QAILGSIVNA 

       190        200        210        220        230        240 
FMVGCMFVKI SQPKKRAETL MFSNNAVISM RDEKLCLMFR VGDLRNSHIV EASIRAKLIK 

       250        260        270        280        290        300 
SRQTKEGEFI PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLEQEEFE 

       310        320        330        340        350        360 
VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT FHDTYETNTP 

       370        380        390        400        410 
SCCAKELAEM KRNGQLLQSL PSPPLLGGCA EAEKEAEAEH DEEEEPNGLS VSRATRGSM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional expression of a rat heart KATP channel." Ashford M.L.J., Bond C.T., Blair T.A., Adelman J.P. Nature 370:456-459(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Heart.
[2]	Erratum Ashford M.L.J., Bond C.T., Blair T.A., Adelman J.P. Nature 378:792-792(1995) [PubMed] [Europe PMC] [Abstract]
[3]	"The G-protein-gated atrial K+ channel IKACh is a heteromultimer of two inwardly rectifying K(+)-channel proteins." Krapivinsky G.B., Gordon E.A., Wickman K., Velimirovic B., Krapivinsky L.D., Clapham D.E. Nature 374:135-141(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 31-39 AND 333-341. Strain: Sprague-Dawley. Tissue: Neonatal heart atrium.
[4]	"Pancreatic islet cells express a family of inwardly rectifying K+ channel subunits which interact to form G-protein-activated channels." Ferrer J., Nichols C.G., Makhina E.N., Salkoff L., Bernstein J., Gerhard D., Wasson J., Ramanadham S., Permutt A. J. Biol. Chem. 270:26086-26091(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreatic islet.
[5]	"Functional characterization and localization of a cardiac-type inwardly rectifying K+ channel." Iizuka M., Kubo Y., Tsunenari I., Pan C.X., Akiba I., Kono T. Recept. Channels 3:299-315(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Heart.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83584 mRNA. Translation: CAA58567.1. L35771 mRNA. Translation: AAC42048.1. D50135 mRNA. Translation: BAA08815.1. BC087022 mRNA. Translation: AAH87022.1.
IPI	IPI00191858.
PIR	S48077.
RefSeq	NP_058993.1. NM_017297.2.
UniGene	Rn.10047.
3D structure databases
ProteinModelPortal	P48548.
SMR	P48548. Positions 197-375.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000048134.
PTM databases
PhosphoSite	P48548.
Proteomic databases
PRIDE	P48548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	29713.
KEGG	rno:29713.
UCSC	RGD:61971. rat.
Organism-specific databases
CTD	3762.
RGD	61971. Kcnj5.
Phylogenomic databases
eggNOG	NOG72812.
HOGENOM	HOG000237325.
HOVERGEN	HBG006178.
InParanoid	P48548.
KO	K04999.
OrthoDB	EOG4VT5X9.
Gene expression databases
Genevestigator	P48548.
Family and domain databases
Gene3D	2.60.40.1400. 1 hit.
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR003277. K_chnl_inward-rec_Kir3.4. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
PANTHER	PTHR11767. PTHR11767. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01330. KIR34CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
NextBio	610153.

Entry information

Entry name

IRK5_RAT

Accession

Primary (citable) accession number: P48548

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents