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Protein

G protein-activated inward rectifier potassium channel 4

Gene

Kcnj5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei179Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 4
Short name:
GIRK-4
Alternative name(s):
Cardiac inward rectifier
Short name:
CIR
Heart KATP channel
Inward rectifier K(+) channel Kir3.4
KATP-1
Potassium channel, inwardly rectifying subfamily J member 5
Gene namesi
Name:Kcnj5
Synonyms:Girk4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104755. Kcnj5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 86CytoplasmicBy similarityAdd BLAST86
Transmembranei87 – 111Helical; Name=M1By similarityAdd BLAST25
Topological domaini112 – 135ExtracellularBy similarityAdd BLAST24
Intramembranei136 – 147Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei148 – 154Pore-formingBy similarity7
Topological domaini155 – 163ExtracellularBy similarity9
Transmembranei164 – 185Helical; Name=M2By similarityAdd BLAST22
Topological domaini186 – 419CytoplasmicBy similarityAdd BLAST234

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi437.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549351 – 419G protein-activated inward rectifier potassium channel 4Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48545.
PaxDbiP48545.
PRIDEiP48545.

PTM databases

iPTMnetiP48545.
PhosphoSitePlusiP48545.

Expressioni

Tissue specificityi

Predominantly atrial and pancreatic expression.

Gene expression databases

BgeeiENSMUSG00000032034.
GenevisibleiP48545. MM.

Interactioni

Subunit structurei

May associate with GIRK1 and GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034533.

Structurei

3D structure databases

ProteinModelPortaliP48545.
SMRiP48545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi149 – 154Selectivity filterBy similarity6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48545.
KOiK04999.
OMAiMNQDMEI.
OrthoDBiEOG091G08HC.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003277. K_chnl_inward-rec_Kir3.4.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01330. KIR34CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P48545-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDSRNAMN QDMEIGVTSQ DHKKIPKQAR DYIPIATDRT RLLTEGKKPR
60 70 80 90 100
QRYMEKTGKC NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTIT
110 120 130 140 150
WLFFGFIWWL IAYVRGDLDH VGDQEWIPCV ENLSGFVSAF LFSIETETTI
160 170 180 190 200
GYGFRVITEK CPEGIILLLV QAILGSIVNA FMVGCMFVKI SQPKKRAETL
210 220 230 240 250
MFSNNAVISM RDEKLCLMFR VGDLRNSHIV EASIRAKLIK SRQTKEGEFI
260 270 280 290 300
PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLEQEEFE
310 320 330 340 350
VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT
360 370 380 390 400
FHDTYETNTP SCCAKELAEM KRSGRLLQYL PSPPLLGGCA EAGNEAEAEK
410
DEEGEPNGLS VSQATRGSM
Length:419
Mass (Da):47,669
Last modified:July 27, 2011 - v3
Checksum:i8383373921C7356A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102L → V in AAB01687 (PubMed:7499385).Curated1
Sequence conflicti109W → G in AAB01687 (PubMed:7499385).Curated1
Sequence conflicti172A → P in AAC53116 (Ref. 2) Curated1
Sequence conflicti193P → S in AAB01687 (PubMed:7499385).Curated1
Sequence conflicti214K → N in AAC53116 (Ref. 2) Curated1
Sequence conflicti232A → V in AAB01687 (PubMed:7499385).Curated1
Sequence conflicti269F → L in AAB01687 (PubMed:7499385).Curated1
Sequence conflicti287W → V in AAB01687 (PubMed:7499385).Curated1
Sequence conflicti296Q → P in AAC53116 (Ref. 2) Curated1
Sequence conflicti351F → S in AAC53116 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33631 mRNA. Translation: AAB01687.1.
AF403131 Genomic DNA. Translation: AAC53116.1.
AK164058 mRNA. Translation: BAE37606.1.
CH466522 Genomic DNA. Translation: EDL25354.1.
CCDSiCCDS22952.1.
RefSeqiNP_034735.3. NM_010605.4.
XP_006510102.1. XM_006510039.3.
XP_017168644.1. XM_017313155.1.
UniGeneiMm.69472.

Genome annotation databases

EnsembliENSMUST00000034533; ENSMUSP00000034533; ENSMUSG00000032034.
GeneIDi16521.
KEGGimmu:16521.
UCSCiuc009orw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33631 mRNA. Translation: AAB01687.1.
AF403131 Genomic DNA. Translation: AAC53116.1.
AK164058 mRNA. Translation: BAE37606.1.
CH466522 Genomic DNA. Translation: EDL25354.1.
CCDSiCCDS22952.1.
RefSeqiNP_034735.3. NM_010605.4.
XP_006510102.1. XM_006510039.3.
XP_017168644.1. XM_017313155.1.
UniGeneiMm.69472.

3D structure databases

ProteinModelPortaliP48545.
SMRiP48545.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034533.

Chemistry databases

GuidetoPHARMACOLOGYi437.

PTM databases

iPTMnetiP48545.
PhosphoSitePlusiP48545.

Proteomic databases

MaxQBiP48545.
PaxDbiP48545.
PRIDEiP48545.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034533; ENSMUSP00000034533; ENSMUSG00000032034.
GeneIDi16521.
KEGGimmu:16521.
UCSCiuc009orw.1. mouse.

Organism-specific databases

CTDi3762.
MGIiMGI:104755. Kcnj5.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48545.
KOiK04999.
OMAiMNQDMEI.
OrthoDBiEOG091G08HC.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

PROiP48545.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032034.
GenevisibleiP48545. MM.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003277. K_chnl_inward-rec_Kir3.4.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01330. KIR34CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNJ5_MOUSE
AccessioniPrimary (citable) accession number: P48545
Secondary accession number(s): P97508, Q3TPX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.