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P48545

- KCNJ5_MOUSE

UniProt

P48545 - KCNJ5_MOUSE

Protein

G protein-activated inward rectifier potassium channel 4

Gene

Kcnj5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei179 – 1791Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. G-protein activated inward rectifier potassium channel activity Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-activated inward rectifier potassium channel 4
    Short name:
    GIRK-4
    Alternative name(s):
    Cardiac inward rectifier
    Short name:
    CIR
    Heart KATP channel
    Inward rectifier K(+) channel Kir3.4
    KATP-1
    Potassium channel, inwardly rectifying subfamily J member 5
    Gene namesi
    Name:Kcnj5
    Synonyms:Girk4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:104755. Kcnj5.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 419419G protein-activated inward rectifier potassium channel 4PRO_0000154935Add
    BLAST

    Proteomic databases

    PRIDEiP48545.

    PTM databases

    PhosphoSiteiP48545.

    Expressioni

    Tissue specificityi

    Predominantly atrial and pancreatic expression.

    Gene expression databases

    BgeeiP48545.
    GenevestigatoriP48545.

    Interactioni

    Subunit structurei

    May associate with GIRK1 and GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP48545.
    SMRiP48545. Positions 51-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8686CytoplasmicBy similarityAdd
    BLAST
    Topological domaini112 – 13524ExtracellularBy similarityAdd
    BLAST
    Topological domaini155 – 1639ExtracellularBy similarity
    Topological domaini186 – 419234CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei136 – 14712Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei148 – 1547Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei87 – 11125Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei164 – 18522Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi149 – 1546Selectivity filterBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    GeneTreeiENSGT00730000110555.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiQ3TPX9.
    KOiK04999.
    OMAiQARSSYM.
    OrthoDBiEOG7XPZ5K.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003277. K_chnl_inward-rec_Kir3.4.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01330. KIR34CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P48545-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGDSRNAMN QDMEIGVTSQ DHKKIPKQAR DYIPIATDRT RLLTEGKKPR    50
    QRYMEKTGKC NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTIT 100
    WLFFGFIWWL IAYVRGDLDH VGDQEWIPCV ENLSGFVSAF LFSIETETTI 150
    GYGFRVITEK CPEGIILLLV QAILGSIVNA FMVGCMFVKI SQPKKRAETL 200
    MFSNNAVISM RDEKLCLMFR VGDLRNSHIV EASIRAKLIK SRQTKEGEFI 250
    PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLEQEEFE 300
    VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT 350
    FHDTYETNTP SCCAKELAEM KRSGRLLQYL PSPPLLGGCA EAGNEAEAEK 400
    DEEGEPNGLS VSQATRGSM 419
    Length:419
    Mass (Da):47,669
    Last modified:July 27, 2011 - v3
    Checksum:i8383373921C7356A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021L → V in AAB01687. (PubMed:7499385)Curated
    Sequence conflicti109 – 1091W → G in AAB01687. (PubMed:7499385)Curated
    Sequence conflicti172 – 1721A → P in AAC53116. 1 PublicationCurated
    Sequence conflicti193 – 1931P → S in AAB01687. (PubMed:7499385)Curated
    Sequence conflicti214 – 2141K → N in AAC53116. 1 PublicationCurated
    Sequence conflicti232 – 2321A → V in AAB01687. (PubMed:7499385)Curated
    Sequence conflicti269 – 2691F → L in AAB01687. (PubMed:7499385)Curated
    Sequence conflicti287 – 2871W → V in AAB01687. (PubMed:7499385)Curated
    Sequence conflicti296 – 2961Q → P in AAC53116. 1 PublicationCurated
    Sequence conflicti351 – 3511F → S in AAC53116. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33631 mRNA. Translation: AAB01687.1.
    AF403131 Genomic DNA. Translation: AAC53116.1.
    AK164058 mRNA. Translation: BAE37606.1.
    CH466522 Genomic DNA. Translation: EDL25354.1.
    CCDSiCCDS22952.1.
    RefSeqiNP_034735.3. NM_010605.4.
    XP_006510102.1. XM_006510039.1.
    XP_006510103.1. XM_006510040.1.
    XP_006510104.1. XM_006510041.1.
    UniGeneiMm.69472.

    Genome annotation databases

    EnsembliENSMUST00000034533; ENSMUSP00000034533; ENSMUSG00000032034.
    GeneIDi16521.
    KEGGimmu:16521.
    UCSCiuc009orw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33631 mRNA. Translation: AAB01687.1 .
    AF403131 Genomic DNA. Translation: AAC53116.1 .
    AK164058 mRNA. Translation: BAE37606.1 .
    CH466522 Genomic DNA. Translation: EDL25354.1 .
    CCDSi CCDS22952.1.
    RefSeqi NP_034735.3. NM_010605.4.
    XP_006510102.1. XM_006510039.1.
    XP_006510103.1. XM_006510040.1.
    XP_006510104.1. XM_006510041.1.
    UniGenei Mm.69472.

    3D structure databases

    ProteinModelPortali P48545.
    SMRi P48545. Positions 51-370.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    GuidetoPHARMACOLOGYi 437.

    PTM databases

    PhosphoSitei P48545.

    Proteomic databases

    PRIDEi P48545.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034533 ; ENSMUSP00000034533 ; ENSMUSG00000032034 .
    GeneIDi 16521.
    KEGGi mmu:16521.
    UCSCi uc009orw.1. mouse.

    Organism-specific databases

    CTDi 3762.
    MGIi MGI:104755. Kcnj5.

    Phylogenomic databases

    eggNOGi NOG72812.
    GeneTreei ENSGT00730000110555.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi Q3TPX9.
    KOi K04999.
    OMAi QARSSYM.
    OrthoDBi EOG7XPZ5K.
    TreeFami TF313676.

    Enzyme and pathway databases

    Reactomei REACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Miscellaneous databases

    NextBioi 289897.
    PROi P48545.
    SOURCEi Search...

    Gene expression databases

    Bgeei P48545.
    Genevestigatori P48545.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003277. K_chnl_inward-rec_Kir3.4.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01330. KIR34CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular properties of neuronal G-protein-activated inwardly rectifying K+ channels."
      Lesage F., Guillemare E., Fink M., Duprat F., Heurteaux C., Fosset M., Romey G., Barhanin J., Lazdunski M.
      J. Biol. Chem. 270:28660-28667(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. Wickman K.D., James M.R., Seldin M.F., Gendler S.J., Clapham D.E.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/SvJ.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiKCNJ5_MOUSE
    AccessioniPrimary (citable) accession number: P48545
    Secondary accession number(s): P97508, Q3TPX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3