P48545 (IRK5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G protein-activated inward rectifier potassium channel 4
Short name=GIRK-4

Alternative name(s):
Cardiac inward rectifier
Short name=CIR
Heart KATP channel
Inward rectifier K(+) channel Kir3.4
KATP-1
Potassium channel, inwardly rectifying subfamily J member 5

Gene names

Name:	Kcnj5
Synonyms:	Girk4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
Subunit structure	May associate with GIRK1 and GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Predominantly atrial and pancreatic expression.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein activated inward rectifier potassium channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	G protein-activated inward rectifier potassium channel 4		PRO_0000154935
Regions
	Topological domain	1 – 86	86	Cytoplasmic By similarity
	Transmembrane	87 – 111	25	Helical; Name=M1; By similarity
	Topological domain	112 – 135	24	Extracellular By similarity
	Intramembrane	136 – 147	12	Helical; Pore-forming; Name=H5; By similarity
	Intramembrane	148 – 154	7	Pore-forming; By similarity
	Topological domain	155 – 163	9	Extracellular By similarity
	Transmembrane	164 – 185	22	Helical; Name=M2; By similarity
	Topological domain	186 – 419	234	Cytoplasmic By similarity
	Motif	149 – 154	6	Selectivity filter By similarity
Sites
	Site	179	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity
Experimental info
	Sequence conflict	102	1	L → V in AAB01687. Ref.1
	Sequence conflict	109	1	W → G in AAB01687. Ref.1
	Sequence conflict	172	1	A → P in AAC53116. Ref.2
	Sequence conflict	193	1	P → S in AAB01687. Ref.1
	Sequence conflict	214	1	K → N in AAC53116. Ref.2
	Sequence conflict	232	1	A → V in AAB01687. Ref.1
	Sequence conflict	269	1	F → L in AAB01687. Ref.1
	Sequence conflict	287	1	W → V in AAB01687. Ref.1
	Sequence conflict	296	1	Q → P in AAC53116. Ref.2
	Sequence conflict	351	1	F → S in AAC53116. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P48545 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 8383373921C7356A	FASTA	419	47,669
	10 20 30 40 50 60 MAGDSRNAMN QDMEIGVTSQ DHKKIPKQAR DYIPIATDRT RLLTEGKKPR QRYMEKTGKC 70 80 90 100 110 120 NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTIT WLFFGFIWWL IAYVRGDLDH 130 140 150 160 170 180 VGDQEWIPCV ENLSGFVSAF LFSIETETTI GYGFRVITEK CPEGIILLLV QAILGSIVNA 190 200 210 220 230 240 FMVGCMFVKI SQPKKRAETL MFSNNAVISM RDEKLCLMFR VGDLRNSHIV EASIRAKLIK 250 260 270 280 290 300 SRQTKEGEFI PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLEQEEFE 310 320 330 340 350 360 VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT FHDTYETNTP 370 380 390 400 410 SCCAKELAEM KRSGRLLQYL PSPPLLGGCA EAGNEAEAEK DEEGEPNGLS VSQATRGSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular properties of neuronal G-protein-activated inwardly rectifying K+ channels." Lesage F., Guillemare E., Fink M., Duprat F., Heurteaux C., Fosset M., Romey G., Barhanin J., Lazdunski M. J. Biol. Chem. 270:28660-28667(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	Wickman K.D., James M.R., Seldin M.F., Gendler S.J., Clapham D.E. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33631 mRNA. Translation: AAB01687.1. AF403131 Genomic DNA. Translation: AAC53116.1. AK164058 mRNA. Translation: BAE37606.1. CH466522 Genomic DNA. Translation: EDL25354.1.
IPI	IPI00113279.
RefSeq	NP_034735.3. NM_010605.4.
UniGene	Mm.69472.
3D structure databases
ProteinModelPortal	P48545.
SMR	P48545. Positions 51-370.
ModBase	Search...
PTM databases
PhosphoSite	P48545.
Proteomic databases
PRIDE	P48545.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034533; ENSMUSP00000034533; ENSMUSG00000032034.
GeneID	16521.
KEGG	mmu:16521.
Organism-specific databases
CTD	3762.
MGI	MGI:104755. Kcnj5.
Phylogenomic databases
eggNOG	NOG72812.
GeneTree	ENSGT00690000101708.
HOGENOM	HOG000237325.
HOVERGEN	HBG006178.
InParanoid	Q3TPX9.
KO	K04999.
OMA	QARSSYM.
OrthoDB	EOG4VT5X9.
Gene expression databases
ArrayExpress	P48545.
Bgee	P48545.
Genevestigator	P48545.
GermOnline	ENSMUSG00000032034. Mus musculus.
Family and domain databases
Gene3D	2.60.40.1400. 1 hit.
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR003277. K_chnl_inward-rec_Kir3.4. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
PANTHER	PTHR11767. PTHR11767. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01330. KIR34CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
NextBio	289897.
SOURCE	Search...

Entry information

Entry name

IRK5_MOUSE

Accession

Primary (citable) accession number: P48545
Secondary accession number(s): P97508, Q3TPX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program