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P48543

- KCNJ9_MOUSE

UniProt

P48543 - KCNJ9_MOUSE

Protein

G protein-activated inward rectifier potassium channel 3

Gene

Kcnj9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei150 – 1501Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. G-protein activated inward rectifier potassium channel activity Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-activated inward rectifier potassium channel 3
    Short name:
    GIRK-3
    Alternative name(s):
    Inward rectifier K(+) channel Kir3.3
    Potassium channel, inwardly rectifying subfamily J member 9
    Gene namesi
    Name:Kcnj9
    Synonyms:Girk3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:108007. Kcnj9.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 393393G protein-activated inward rectifier potassium channel 3PRO_0000154951Add
    BLAST

    Proteomic databases

    PaxDbiP48543.
    PRIDEiP48543.

    PTM databases

    PhosphoSiteiP48543.

    Expressioni

    Tissue specificityi

    Expressed mainly in the brain, some expression in the skeletal muscle.

    Gene expression databases

    ArrayExpressiP48543.
    BgeeiP48543.
    GenevestigatoriP48543.

    Interactioni

    Subunit structurei

    Associates with GIRK1 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger. When alone, fail to give functional channels in Xenopus oocytes. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane.

    Structurei

    3D structure databases

    ProteinModelPortaliP48543.
    SMRiP48543. Positions 22-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5757CytoplasmicBy similarityAdd
    BLAST
    Topological domaini83 – 10624ExtracellularBy similarityAdd
    BLAST
    Topological domaini126 – 1349ExtracellularBy similarity
    Topological domaini157 – 393237CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei107 – 11812Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei119 – 1257Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei58 – 8225Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei135 – 15622Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi120 – 1256Selectivity filterBy similarity
    Motifi390 – 3934PDZ-binding

    Domaini

    The PDZ-binding motif specifically binds the PDZ domain of SNX27: the specificity for SNX27 is provided by the 2 residues located upstream (Glu-388 and Ser-389) of the PDZ-binding motif.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiP48543.
    KOiK05002.
    OMAiDYASFHQ.
    OrthoDBiEOG7XPZ5K.
    PhylomeDBiP48543.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003276. K_chnl_inward-rec_Kir3.3.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF17. PTHR11767:SF17. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01329. KIR33CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P48543-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQENAAFSP GSEEPPRRRG RQRYVEKDGR CNVQQGNVRE TYRYLTDLFT    50
    TLVDLQWRLS LLFFVLAYAL TWLFFGAIWW LIAYGRGDLE HLEDTAWTPC 100
    VNNLNGFVAA FLFSIETETT IGYGHRVITD QCPEGIVLLL LQAILGSMVN 150
    AFMVGCMFVK ISQPNKRAAT LVFSSHAVVS LRDGRLCLMF RVGDLRSSHI 200
    VEASIRAKLI RSRQTLEGEF IPLHQTDLSV GFDTGDDRLF LVSPLVISHE 250
    IDAASPFWEA SRRALERDDF EIVVILEGMV EATGMTCQAR SSYLVDEVLW 300
    GHRFTSVLTL EDGFYEVDYA SFHETFEVPT PSCSARELAE AAARLDAHLY 350
    WSIPSRLDEK VEEEGAGEGA GAGDGADKEH NGCLPPPESE SKV 393
    Length:393
    Mass (Da):43,974
    Last modified:April 27, 2001 - v2
    Checksum:i9CF749672A865B08
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti60 – 601S → R in AAA53246. (PubMed:7926018)Curated
    Sequence conflicti77 – 771A → V in AAA53246. (PubMed:7926018)Curated
    Sequence conflicti370 – 39324AGAGD…SESKV → GRCGRWS in AAA53246. (PubMed:7926018)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U11860 mRNA. Translation: AAA53246.1.
    AF130860 mRNA. Translation: AAD31016.1.
    BC065161 mRNA. Translation: AAH65161.1.
    CCDSiCCDS35783.1.
    PIRiS48739.
    RefSeqiNP_032455.2. NM_008429.2.
    XP_006496741.1. XM_006496678.1.
    UniGeneiMm.261168.

    Genome annotation databases

    EnsembliENSMUST00000062387; ENSMUSP00000060110; ENSMUSG00000038026.
    GeneIDi16524.
    KEGGimmu:16524.
    UCSCiuc007dqg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U11860 mRNA. Translation: AAA53246.1 .
    AF130860 mRNA. Translation: AAD31016.1 .
    BC065161 mRNA. Translation: AAH65161.1 .
    CCDSi CCDS35783.1.
    PIRi S48739.
    RefSeqi NP_032455.2. NM_008429.2.
    XP_006496741.1. XM_006496678.1.
    UniGenei Mm.261168.

    3D structure databases

    ProteinModelPortali P48543.
    SMRi P48543. Positions 22-348.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P48543.

    Proteomic databases

    PaxDbi P48543.
    PRIDEi P48543.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000062387 ; ENSMUSP00000060110 ; ENSMUSG00000038026 .
    GeneIDi 16524.
    KEGGi mmu:16524.
    UCSCi uc007dqg.1. mouse.

    Organism-specific databases

    CTDi 3765.
    MGIi MGI:108007. Kcnj9.

    Phylogenomic databases

    eggNOGi NOG72812.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi P48543.
    KOi K05002.
    OMAi DYASFHQ.
    OrthoDBi EOG7XPZ5K.
    PhylomeDBi P48543.
    TreeFami TF313676.

    Enzyme and pathway databases

    Reactomei REACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Miscellaneous databases

    NextBioi 289915.
    PROi P48543.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P48543.
    Bgeei P48543.
    Genevestigatori P48543.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003276. K_chnl_inward-rec_Kir3.3.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF17. PTHR11767:SF17. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01329. KIR33CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning provides evidence for a family of inward rectifier and G-protein coupled K+ channels in the brain."
      Lesage F., Duprat F., Fink M., Guillemare E., Coppola T., Lazdunski M., Hugnot J.-P.
      FEBS Lett. 353:37-42(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Functional expression and characterization of G-protein-gated inwardly rectifying K+ channels containing GIRK3."
      Jelacic T.M., Sims S.M., Clapham D.E.
      J. Membr. Biol. 169:123-129(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiKCNJ9_MOUSE
    AccessioniPrimary (citable) accession number: P48543
    Secondary accession number(s): Q9WUE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3