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P48543 (KCNJ9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-activated inward rectifier potassium channel 3

Short name=GIRK-3
Alternative name(s):
Inward rectifier K(+) channel Kir3.3
Potassium channel, inwardly rectifying subfamily J member 9
Gene names
Name:Kcnj9
Synonyms:Girk3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

Subunit structure

Associates with GIRK1 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger. When alone, fail to give functional channels in Xenopus oocytes. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed mainly in the brain, some expression in the skeletal muscle.

Domain

The PDZ-binding motif specifically binds the PDZ domain of SNX27: the specificity for SNX27 is provided by the 2 residues located upstream (Glu-388 and Ser-389) of the PDZ-binding motif By similarity.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ9 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393G protein-activated inward rectifier potassium channel 3
PRO_0000154951

Regions

Topological domain1 – 5757Cytoplasmic By similarity
Transmembrane58 – 8225Helical; Name=M1; By similarity
Topological domain83 – 10624Extracellular By similarity
Intramembrane107 – 11812Helical; Pore-forming; Name=H5; By similarity
Intramembrane119 – 1257Pore-forming; By similarity
Topological domain126 – 1349Extracellular By similarity
Transmembrane135 – 15622Helical; Name=M2; By similarity
Topological domain157 – 393237Cytoplasmic By similarity
Motif120 – 1256Selectivity filter By similarity
Motif390 – 3934PDZ-binding

Sites

Site1501Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Experimental info

Sequence conflict601S → R in AAA53246. Ref.1
Sequence conflict771A → V in AAA53246. Ref.1
Sequence conflict370 – 39324AGAGD…SESKV → GRCGRWS in AAA53246. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48543 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 9CF749672A865B08

FASTA39343,974
        10         20         30         40         50         60 
MAQENAAFSP GSEEPPRRRG RQRYVEKDGR CNVQQGNVRE TYRYLTDLFT TLVDLQWRLS 

        70         80         90        100        110        120 
LLFFVLAYAL TWLFFGAIWW LIAYGRGDLE HLEDTAWTPC VNNLNGFVAA FLFSIETETT 

       130        140        150        160        170        180 
IGYGHRVITD QCPEGIVLLL LQAILGSMVN AFMVGCMFVK ISQPNKRAAT LVFSSHAVVS 

       190        200        210        220        230        240 
LRDGRLCLMF RVGDLRSSHI VEASIRAKLI RSRQTLEGEF IPLHQTDLSV GFDTGDDRLF 

       250        260        270        280        290        300 
LVSPLVISHE IDAASPFWEA SRRALERDDF EIVVILEGMV EATGMTCQAR SSYLVDEVLW 

       310        320        330        340        350        360 
GHRFTSVLTL EDGFYEVDYA SFHETFEVPT PSCSARELAE AAARLDAHLY WSIPSRLDEK 

       370        380        390 
VEEEGAGEGA GAGDGADKEH NGCLPPPESE SKV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning provides evidence for a family of inward rectifier and G-protein coupled K+ channels in the brain."
Lesage F., Duprat F., Fink M., Guillemare E., Coppola T., Lazdunski M., Hugnot J.-P.
FEBS Lett. 353:37-42(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Functional expression and characterization of G-protein-gated inwardly rectifying K+ channels containing GIRK3."
Jelacic T.M., Sims S.M., Clapham D.E.
J. Membr. Biol. 169:123-129(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U11860 mRNA. Translation: AAA53246.1.
AF130860 mRNA. Translation: AAD31016.1.
BC065161 mRNA. Translation: AAH65161.1.
CCDSCCDS35783.1.
PIRS48739.
RefSeqNP_032455.2. NM_008429.2.
XP_006496741.1. XM_006496678.1.
UniGeneMm.261168.

3D structure databases

ProteinModelPortalP48543.
SMRP48543. Positions 22-348.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP48543.

Proteomic databases

PaxDbP48543.
PRIDEP48543.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000062387; ENSMUSP00000060110; ENSMUSG00000038026.
GeneID16524.
KEGGmmu:16524.
UCSCuc007dqg.1. mouse.

Organism-specific databases

CTD3765.
MGIMGI:108007. Kcnj9.

Phylogenomic databases

eggNOGNOG72812.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP48543.
KOK05002.
OMADYASFHQ.
OrthoDBEOG7XPZ5K.
PhylomeDBP48543.
TreeFamTF313676.

Gene expression databases

ArrayExpressP48543.
BgeeP48543.
GenevestigatorP48543.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003276. K_chnl_inward-rec_Kir3.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF17. PTHR11767:SF17. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01329. KIR33CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio289915.
PROP48543.
SOURCESearch...

Entry information

Entry nameKCNJ9_MOUSE
AccessionPrimary (citable) accession number: P48543
Secondary accession number(s): Q9WUE1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot