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Protein

G protein-activated inward rectifier potassium channel 3

Gene

Kcnj9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei150Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-MMU-1296041 Activation of G protein gated Potassium channels
R-MMU-997272 Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 3
Short name:
GIRK-3
Alternative name(s):
Inward rectifier K(+) channel Kir3.3
Potassium channel, inwardly rectifying subfamily J member 9
Gene namesi
Name:Kcnj9
Synonyms:Girk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:108007 Kcnj9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 57CytoplasmicBy similarityAdd BLAST57
Transmembranei58 – 82Helical; Name=M1By similarityAdd BLAST25
Topological domaini83 – 106ExtracellularBy similarityAdd BLAST24
Intramembranei107 – 118Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei119 – 125Pore-formingBy similarity7
Topological domaini126 – 134ExtracellularBy similarity9
Transmembranei135 – 156Helical; Name=M2By similarityAdd BLAST22
Topological domaini157 – 393CytoplasmicBy similarityAdd BLAST237

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549511 – 393G protein-activated inward rectifier potassium channel 3Add BLAST393

Proteomic databases

PaxDbiP48543
PeptideAtlasiP48543
PRIDEiP48543

PTM databases

PhosphoSitePlusiP48543

Expressioni

Tissue specificityi

Expressed mainly in the brain, some expression in the skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000038026
ExpressionAtlasiP48543 baseline and differential
GenevisibleiP48543 MM

Interactioni

Subunit structurei

Associates with GIRK1 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger. When alone, fail to give functional channels in Xenopus oocytes. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060110

Structurei

3D structure databases

ProteinModelPortaliP48543
SMRiP48543
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi120 – 125Selectivity filterBy similarity6
Motifi390 – 393PDZ-binding4

Domaini

The PDZ-binding motif specifically binds the PDZ domain of SNX27: the specificity for SNX27 is provided by the 2 residues located upstream (Glu-388 and Ser-389) of the PDZ-binding motif.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827 Eukaryota
ENOG410XQ62 LUCA
GeneTreeiENSGT00760000118842
HOGENOMiHOG000237325
HOVERGENiHBG006178
InParanoidiP48543
KOiK05002
OMAiDGKCNVQ
OrthoDBiEOG091G08HC
PhylomeDBiP48543
TreeFamiTF313676

Family and domain databases

Gene3Di2.60.40.1400, 2 hits
InterProiView protein in InterPro
IPR014756 Ig_E-set
IPR016449 K_chnl_inward-rec_Kir
IPR003276 K_chnl_inward-rec_Kir3.3
IPR013518 K_chnl_inward-rec_Kir_cyto
PANTHERiPTHR11767 PTHR11767, 1 hit
PTHR11767:SF17 PTHR11767:SF17, 1 hit
PfamiView protein in Pfam
PF01007 IRK, 1 hit
PIRSFiPIRSF005465 GIRK_kir, 1 hit
PRINTSiPR01329 KIR33CHANNEL
PR01320 KIRCHANNEL
SUPFAMiSSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

P48543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQENAAFSP GSEEPPRRRG RQRYVEKDGR CNVQQGNVRE TYRYLTDLFT
60 70 80 90 100
TLVDLQWRLS LLFFVLAYAL TWLFFGAIWW LIAYGRGDLE HLEDTAWTPC
110 120 130 140 150
VNNLNGFVAA FLFSIETETT IGYGHRVITD QCPEGIVLLL LQAILGSMVN
160 170 180 190 200
AFMVGCMFVK ISQPNKRAAT LVFSSHAVVS LRDGRLCLMF RVGDLRSSHI
210 220 230 240 250
VEASIRAKLI RSRQTLEGEF IPLHQTDLSV GFDTGDDRLF LVSPLVISHE
260 270 280 290 300
IDAASPFWEA SRRALERDDF EIVVILEGMV EATGMTCQAR SSYLVDEVLW
310 320 330 340 350
GHRFTSVLTL EDGFYEVDYA SFHETFEVPT PSCSARELAE AAARLDAHLY
360 370 380 390
WSIPSRLDEK VEEEGAGEGA GAGDGADKEH NGCLPPPESE SKV
Length:393
Mass (Da):43,974
Last modified:April 27, 2001 - v2
Checksum:i9CF749672A865B08
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60S → R in AAA53246 (PubMed:7926018).Curated1
Sequence conflicti77A → V in AAA53246 (PubMed:7926018).Curated1
Sequence conflicti370 – 393AGAGD…SESKV → GRCGRWS in AAA53246 (PubMed:7926018).CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11860 mRNA Translation: AAA53246.1
AF130860 mRNA Translation: AAD31016.1
BC065161 mRNA Translation: AAH65161.1
CCDSiCCDS35783.1
PIRiS48739
RefSeqiNP_032455.2, NM_008429.2
XP_006496741.1, XM_006496678.3
UniGeneiMm.261168

Genome annotation databases

EnsembliENSMUST00000062387; ENSMUSP00000060110; ENSMUSG00000038026
ENSMUST00000194204; ENSMUSP00000141633; ENSMUSG00000038026
GeneIDi16524
KEGGimmu:16524
UCSCiuc007dqg.1 mouse

Similar proteinsi

Entry informationi

Entry nameiKCNJ9_MOUSE
AccessioniPrimary (citable) accession number: P48543
Secondary accession number(s): Q9WUE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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