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Protein

G protein-activated inward rectifier potassium channel 3

Gene

Kcnj9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei150 – 1501Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. G-protein activated inward rectifier potassium channel activity Source: GO_Central

GO - Biological processi

  1. potassium ion import Source: GO_Central
  2. regulation of ion transmembrane transport Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_308732. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_339334. Activation of G protein gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 3
Short name:
GIRK-3
Alternative name(s):
Inward rectifier K(+) channel Kir3.3
Potassium channel, inwardly rectifying subfamily J member 9
Gene namesi
Name:Kcnj9
Synonyms:Girk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:108007. Kcnj9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757CytoplasmicBy similarityAdd
BLAST
Transmembranei58 – 8225Helical; Name=M1By similarityAdd
BLAST
Topological domaini83 – 10624ExtracellularBy similarityAdd
BLAST
Intramembranei107 – 11812Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei119 – 1257Pore-formingBy similarity
Topological domaini126 – 1349ExtracellularBy similarity
Transmembranei135 – 15622Helical; Name=M2By similarityAdd
BLAST
Topological domaini157 – 393237CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393G protein-activated inward rectifier potassium channel 3PRO_0000154951Add
BLAST

Proteomic databases

PaxDbiP48543.
PRIDEiP48543.

PTM databases

PhosphoSiteiP48543.

Expressioni

Tissue specificityi

Expressed mainly in the brain, some expression in the skeletal muscle.

Gene expression databases

BgeeiP48543.
ExpressionAtlasiP48543. baseline and differential.
GenevestigatoriP48543.

Interactioni

Subunit structurei

Associates with GIRK1 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger. When alone, fail to give functional channels in Xenopus oocytes. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane.

Structurei

3D structure databases

ProteinModelPortaliP48543.
SMRiP48543. Positions 22-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi120 – 1256Selectivity filterBy similarity
Motifi390 – 3934PDZ-binding

Domaini

The PDZ-binding motif specifically binds the PDZ domain of SNX27: the specificity for SNX27 is provided by the 2 residues located upstream (Glu-388 and Ser-389) of the PDZ-binding motif.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48543.
KOiK05002.
OMAiYVEKNGR.
OrthoDBiEOG7XPZ5K.
PhylomeDBiP48543.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003276. K_chnl_inward-rec_Kir3.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF17. PTHR11767:SF17. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01329. KIR33CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P48543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQENAAFSP GSEEPPRRRG RQRYVEKDGR CNVQQGNVRE TYRYLTDLFT
60 70 80 90 100
TLVDLQWRLS LLFFVLAYAL TWLFFGAIWW LIAYGRGDLE HLEDTAWTPC
110 120 130 140 150
VNNLNGFVAA FLFSIETETT IGYGHRVITD QCPEGIVLLL LQAILGSMVN
160 170 180 190 200
AFMVGCMFVK ISQPNKRAAT LVFSSHAVVS LRDGRLCLMF RVGDLRSSHI
210 220 230 240 250
VEASIRAKLI RSRQTLEGEF IPLHQTDLSV GFDTGDDRLF LVSPLVISHE
260 270 280 290 300
IDAASPFWEA SRRALERDDF EIVVILEGMV EATGMTCQAR SSYLVDEVLW
310 320 330 340 350
GHRFTSVLTL EDGFYEVDYA SFHETFEVPT PSCSARELAE AAARLDAHLY
360 370 380 390
WSIPSRLDEK VEEEGAGEGA GAGDGADKEH NGCLPPPESE SKV
Length:393
Mass (Da):43,974
Last modified:April 26, 2001 - v2
Checksum:i9CF749672A865B08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601S → R in AAA53246 (PubMed:7926018).Curated
Sequence conflicti77 – 771A → V in AAA53246 (PubMed:7926018).Curated
Sequence conflicti370 – 39324AGAGD…SESKV → GRCGRWS in AAA53246 (PubMed:7926018).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11860 mRNA. Translation: AAA53246.1.
AF130860 mRNA. Translation: AAD31016.1.
BC065161 mRNA. Translation: AAH65161.1.
CCDSiCCDS35783.1.
PIRiS48739.
RefSeqiNP_032455.2. NM_008429.2.
XP_006496741.1. XM_006496678.2.
UniGeneiMm.261168.

Genome annotation databases

EnsembliENSMUST00000062387; ENSMUSP00000060110; ENSMUSG00000038026.
ENSMUST00000194204; ENSMUSP00000141633; ENSMUSG00000038026.
GeneIDi16524.
KEGGimmu:16524.
UCSCiuc007dqg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11860 mRNA. Translation: AAA53246.1.
AF130860 mRNA. Translation: AAD31016.1.
BC065161 mRNA. Translation: AAH65161.1.
CCDSiCCDS35783.1.
PIRiS48739.
RefSeqiNP_032455.2. NM_008429.2.
XP_006496741.1. XM_006496678.2.
UniGeneiMm.261168.

3D structure databases

ProteinModelPortaliP48543.
SMRiP48543. Positions 22-348.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP48543.

Proteomic databases

PaxDbiP48543.
PRIDEiP48543.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062387; ENSMUSP00000060110; ENSMUSG00000038026.
ENSMUST00000194204; ENSMUSP00000141633; ENSMUSG00000038026.
GeneIDi16524.
KEGGimmu:16524.
UCSCiuc007dqg.1. mouse.

Organism-specific databases

CTDi3765.
MGIiMGI:108007. Kcnj9.

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP48543.
KOiK05002.
OMAiYVEKNGR.
OrthoDBiEOG7XPZ5K.
PhylomeDBiP48543.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiREACT_308732. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_339334. Activation of G protein gated Potassium channels.

Miscellaneous databases

NextBioi289915.
PROiP48543.
SOURCEiSearch...

Gene expression databases

BgeeiP48543.
ExpressionAtlasiP48543. baseline and differential.
GenevestigatoriP48543.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003276. K_chnl_inward-rec_Kir3.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF17. PTHR11767:SF17. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01329. KIR33CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning provides evidence for a family of inward rectifier and G-protein coupled K+ channels in the brain."
    Lesage F., Duprat F., Fink M., Guillemare E., Coppola T., Lazdunski M., Hugnot J.-P.
    FEBS Lett. 353:37-42(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Functional expression and characterization of G-protein-gated inwardly rectifying K+ channels containing GIRK3."
    Jelacic T.M., Sims S.M., Clapham D.E.
    J. Membr. Biol. 169:123-129(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiKCNJ9_MOUSE
AccessioniPrimary (citable) accession number: P48543
Secondary accession number(s): Q9WUE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: April 26, 2001
Last modified: March 31, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.