P48537 (CRTI_CERNC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytoene desaturase
EC=1.3.99.30

Alternative name(s):
Phytoene desaturase (3,4-didehydrolycopene-forming)

Gene names

Name:

PDH1

Organism

Cercospora nicotianae (Barn spot disease fungus)

Taxonomic identifier

29003 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › dothideomyceta › Dothideomycetes › Dothideomycetidae › Capnodiales › Mycosphaerellaceae › mitosporic Mycosphaerellaceae › Cercospora

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phytoene desaturase involved in the carotenoid biosynthesis pathway. Converts phytoene into 3,4-didehydrolycopene via the intermediary of phytofluene, zeta-carotene, neurosporene and lycopene, by introducing up to five double bonds into phytoene By similarity.
Catalytic activity	15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor.
Cofactor	NAD By similarity.
Pathway	Carotenoid biosynthesis; lycopene biosynthesis.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the carotenoid/retinoid oxidoreductase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 621	598	Phytoene desaturase		PRO_0000067694
Regions
	Transmembrane	598 – 618	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P48537 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 61B9EA7784963CA8	FASTA	621	69,530
	10 20 30 40 50 60 MPSTSKRPTA IVIGSGVGGV STAARLARAG FHVTVLEKNN FTGGRCSLIH HEGYRFDQGP 70 80 90 100 110 120 SLLLLPGLFH RTFAELGTSL EQEGVKLLKC EPNYMIHFSD GEKFTLSSDL SVMKTEVEKW 130 140 150 160 170 180 EGKEGYTRYL EFLKESHGHY ELSVREVLLR NFEGLTAMLR PEFLRHLLQL HPFESIWTRA 190 200 210 220 230 240 GKYFWTERLR RVFTFGSMYM GMSPFDAPGT YSLLQYTELA EGIWYPVGGF HRVVEALVKI 250 260 270 280 290 300 GEREGVDFRM ETAVKKILLS EDGGVAKGVE LEDGRRLEAD VVVNNSDLVY AYEKLLPIKT 310 320 330 340 350 360 PYAESLKGRP GSCSSISFYW ALDRQVPELE AHNIFLADEY RESFDSIFKK HLIPDEPSFY 370 380 390 400 410 420 VNVPSRVDST AAPEGKDSVV VLVPVGHLLE EDRHASQAHQ LSASRNGHIS SASPPDQPGL 430 440 450 460 470 480 TPTEKQDWPA MISLARKTIL STIQSRTNVD LTPLIIHEST NSPLSWKQTF NLDRGAILGL 490 500 510 520 530 540 SHSFFNVLCF RPTTRARKPG AFDAQLLKFG VLGRAAEVII DAFRGRGKDI KGLYMVGASA 550 560 570 580 590 600 HPGTGVPICL AGGALVAEQI CGDYGVDIPW KEEERKGRDV GGKRKLDVLE SPMWLDSWEQ 610 620 WVSVLIYLLV GIFAWLWMKF R

References

[1]	"Isolation, sequence, and characterization of the Cercospora nicotianae phytoene dehydrogenase gene." Ehrenshaft M., Daub M.E. Appl. Environ. Microbiol. 60:2766-2771(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 18366.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U03903 Genomic DNA. Translation: AAB86988.1.
PIR	T48646.
3D structure databases
ProteinModelPortal	P48537.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00803.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. IPR014105. CrtI_fam. IPR008150. Phytoene_DH_bac_CS. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02734. crtI_fam. 1 hit.
PROSITE	PS00982. PHYTOENE_DH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTI_CERNC

Accession

Primary (citable) accession number: P48537

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program