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P48534

- APX1_PEA

UniProt

P48534 - APX1_PEA

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Protein

L-ascorbate peroxidase, cytosolic

Gene

APX1

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a key role in hydrogen peroxide removal.

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

Binds 1 heme B (iron-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei38 – 381Transition state stabilizer
Active sitei42 – 421Proton acceptor
Metal bindingi163 – 1631Iron (heme axial ligand)1 PublicationPROSITE-ProRule annotation
Metal bindingi164 – 1641Potassium or calcium
Metal bindingi180 – 1801Potassium or calcium
Metal bindingi182 – 1821Potassium or calcium
Metal bindingi185 – 1851Potassium or calcium; via carbonyl oxygen
Metal bindingi187 – 1871Potassium or calcium

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. L-ascorbate peroxidase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. hydrogen peroxide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Stress response

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

SABIO-RKP48534.

Protein family/group databases

PeroxiBasei2462. PsAPx01.

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase, cytosolic (EC:1.11.1.11)
Short name:
AP
Alternative name(s):
PsAPx01
Gene namesi
Name:APX1
Synonyms:APPX1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 250249L-ascorbate peroxidase, cytosolicPRO_0000055597Add
BLAST

Proteomic databases

ProMEXiP48534.

Expressioni

Inductioni

By stress.1 Publication

Structurei

Secondary structure

1
250
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 3021Combined sources
Helixi33 – 4412Combined sources
Turni49 – 524Combined sources
Beta strandi55 – 584Combined sources
Helixi59 – 613Combined sources
Helixi63 – 664Combined sources
Helixi69 – 713Combined sources
Helixi74 – 8613Combined sources
Helixi93 – 10715Combined sources
Helixi138 – 1458Combined sources
Turni146 – 1483Combined sources
Helixi153 – 1608Combined sources
Helixi161 – 1644Combined sources
Turni170 – 1723Combined sources
Beta strandi177 – 1815Combined sources
Helixi189 – 1957Combined sources
Helixi206 – 2094Combined sources
Turni210 – 2123Combined sources
Helixi217 – 22610Combined sources
Helixi228 – 24316Combined sources
Turni244 – 2463Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1APXX-ray2.20A/B/C/D2-250[»]
ProteinModelPortaliP48534.
SMRiP48534. Positions 2-250.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP48534.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P48534-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKSYPTVSP DYQKAIEKAK RKLRGFIAEK KCAPLILRLA WHSAGTFDSK
60 70 80 90 100
TKTGGPFGTI KHQAELAHGA NNGLDIAVRL LEPIKEQFPI VSYADFYQLA
110 120 130 140 150
GVVAVEITGG PEVPFHPGRE DKPEPPPEGR LPDATKGSDH LRDVFGKAMG
160 170 180 190 200
LSDQDIVALS GGHTIGAAHK ERSGFEGPWT SNPLIFDNSY FTELLTGEKD
210 220 230 240 250
GLLQLPSDKA LLTDSVFRPL VEKYAADEDV FFADYAEAHL KLSELGFAEA
Length:250
Mass (Da):27,193
Last modified:January 23, 2007 - v2
Checksum:i6F51006D0A13B42C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93051 Genomic DNA. Translation: AAA33645.1.
X62077 mRNA. Translation: CAA43992.1.
PIRiA45116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93051 Genomic DNA. Translation: AAA33645.1 .
X62077 mRNA. Translation: CAA43992.1 .
PIRi A45116.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1APX X-ray 2.20 A/B/C/D 2-250 [» ]
ProteinModelPortali P48534.
SMRi P48534. Positions 2-250.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

PeroxiBasei 2462. PsAPx01.

Proteomic databases

ProMEXi P48534.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P48534.

Miscellaneous databases

EvolutionaryTracei P48534.

Family and domain databases

InterProi IPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view ]
Pfami PF00141. peroxidase. 1 hit.
[Graphical view ]
PRINTSi PR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMi SSF48113. SSF48113. 1 hit.
PROSITEi PS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence analysis of a cDNA encoding pea cytosolic ascorbate peroxidase."
    Mittler R., Zilinskas B.A.
    FEBS Lett. 289:257-259(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Little Marvel.
    Tissue: Leaf.
  2. "Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase."
    Mittler R., Zilinskas B.A.
    J. Biol. Chem. 267:21802-21807(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Strain: cv. Little Marvel.
  3. Erratum
    Mittler R., Zilinskas B.A.
    J. Biol. Chem. 268:4568-4568(1993)
  4. "Crystal structure of recombinant pea cytosolic ascorbate peroxidase."
    Patterson W.R., Poulos T.L.
    Biochemistry 34:4331-4341(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH HEME AND POTASSIUM IONS.

Entry informationi

Entry nameiAPX1_PEA
AccessioniPrimary (citable) accession number: P48534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3