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P48534

- APX1_PEA

UniProt

P48534 - APX1_PEA

Protein

L-ascorbate peroxidase, cytosolic

Gene

APX1

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Plays a key role in hydrogen peroxide removal.

    Catalytic activityi

    2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

    Cofactori

    Binds 1 heme B (iron-protoporphyrin IX) group per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei38 – 381Transition state stabilizer
    Active sitei42 – 421Proton acceptor
    Metal bindingi163 – 1631Iron (heme axial ligand)1 PublicationPROSITE-ProRule annotation
    Metal bindingi164 – 1641Potassium or calcium
    Metal bindingi180 – 1801Potassium or calcium
    Metal bindingi182 – 1821Potassium or calcium
    Metal bindingi185 – 1851Potassium or calcium; via carbonyl oxygen
    Metal bindingi187 – 1871Potassium or calcium

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. L-ascorbate peroxidase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. hydrogen peroxide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Biological processi

    Hydrogen peroxide, Stress response

    Keywords - Ligandi

    Calcium, Heme, Iron, Metal-binding, Potassium

    Enzyme and pathway databases

    SABIO-RKP48534.

    Protein family/group databases

    PeroxiBasei2462. PsAPx01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-ascorbate peroxidase, cytosolic (EC:1.11.1.11)
    Short name:
    AP
    Alternative name(s):
    PsAPx01
    Gene namesi
    Name:APX1
    Synonyms:APPX1
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 250249L-ascorbate peroxidase, cytosolicPRO_0000055597Add
    BLAST

    Proteomic databases

    ProMEXiP48534.

    Expressioni

    Inductioni

    By stress.1 Publication

    Structurei

    Secondary structure

    1
    250
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 3021
    Helixi33 – 4412
    Turni49 – 524
    Beta strandi55 – 584
    Helixi59 – 613
    Helixi63 – 664
    Helixi69 – 713
    Helixi74 – 8613
    Helixi93 – 10715
    Helixi138 – 1458
    Turni146 – 1483
    Helixi153 – 1608
    Helixi161 – 1644
    Turni170 – 1723
    Beta strandi177 – 1815
    Helixi189 – 1957
    Helixi206 – 2094
    Turni210 – 2123
    Helixi217 – 22610
    Helixi228 – 24316
    Turni244 – 2463

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1APXX-ray2.20A/B/C/D2-250[»]
    ProteinModelPortaliP48534.
    SMRiP48534. Positions 2-250.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP48534.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR002207. Asc_peroxidase.
    IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR019794. Peroxidases_AS.
    IPR019793. Peroxidases_heam-ligand_BS.
    [Graphical view]
    PfamiPF00141. peroxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00459. ASPEROXIDASE.
    PR00458. PEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 1 hit.
    PROSITEiPS00435. PEROXIDASE_1. 1 hit.
    PS00436. PEROXIDASE_2. 1 hit.
    PS50873. PEROXIDASE_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P48534-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKSYPTVSP DYQKAIEKAK RKLRGFIAEK KCAPLILRLA WHSAGTFDSK    50
    TKTGGPFGTI KHQAELAHGA NNGLDIAVRL LEPIKEQFPI VSYADFYQLA 100
    GVVAVEITGG PEVPFHPGRE DKPEPPPEGR LPDATKGSDH LRDVFGKAMG 150
    LSDQDIVALS GGHTIGAAHK ERSGFEGPWT SNPLIFDNSY FTELLTGEKD 200
    GLLQLPSDKA LLTDSVFRPL VEKYAADEDV FFADYAEAHL KLSELGFAEA 250
    Length:250
    Mass (Da):27,193
    Last modified:January 23, 2007 - v2
    Checksum:i6F51006D0A13B42C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93051 Genomic DNA. Translation: AAA33645.1.
    X62077 mRNA. Translation: CAA43992.1.
    PIRiA45116.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93051 Genomic DNA. Translation: AAA33645.1 .
    X62077 mRNA. Translation: CAA43992.1 .
    PIRi A45116.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1APX X-ray 2.20 A/B/C/D 2-250 [» ]
    ProteinModelPortali P48534.
    SMRi P48534. Positions 2-250.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    PeroxiBasei 2462. PsAPx01.

    Proteomic databases

    ProMEXi P48534.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P48534.

    Miscellaneous databases

    EvolutionaryTracei P48534.

    Family and domain databases

    InterProi IPR002207. Asc_peroxidase.
    IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR019794. Peroxidases_AS.
    IPR019793. Peroxidases_heam-ligand_BS.
    [Graphical view ]
    Pfami PF00141. peroxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00459. ASPEROXIDASE.
    PR00458. PEROXIDASE.
    SUPFAMi SSF48113. SSF48113. 1 hit.
    PROSITEi PS00435. PEROXIDASE_1. 1 hit.
    PS00436. PEROXIDASE_2. 1 hit.
    PS50873. PEROXIDASE_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence analysis of a cDNA encoding pea cytosolic ascorbate peroxidase."
      Mittler R., Zilinskas B.A.
      FEBS Lett. 289:257-259(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Little Marvel.
      Tissue: Leaf.
    2. "Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase."
      Mittler R., Zilinskas B.A.
      J. Biol. Chem. 267:21802-21807(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
      Strain: cv. Little Marvel.
    3. Erratum
      Mittler R., Zilinskas B.A.
      J. Biol. Chem. 268:4568-4568(1993)
    4. "Crystal structure of recombinant pea cytosolic ascorbate peroxidase."
      Patterson W.R., Poulos T.L.
      Biochemistry 34:4331-4341(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH HEME AND POTASSIUM IONS.

    Entry informationi

    Entry nameiAPX1_PEA
    AccessioniPrimary (citable) accession number: P48534
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Binds one cation per subunit; probably K+, but might also be Ca2+.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3