Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P48534 (APX1_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-ascorbate peroxidase, cytosolic

Short name=AP
EC=1.11.1.11
Alternative name(s):
PsAPx01
Gene names
Name:APX1
Synonyms:APPX1
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a key role in hydrogen peroxide removal.

Catalytic activity

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per subunit.

Subcellular location

Cytoplasm.

Induction

By stress. Ref.2

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.

Sequence similarities

Belongs to the peroxidase family. Ascorbate peroxidase subfamily.

Ontologies

Keywords
   Biological processHydrogen peroxide
Stress response
   Cellular componentCytoplasm
   LigandCalcium
Heme
Iron
Metal-binding
Potassium
   Molecular functionOxidoreductase
Peroxidase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processhydrogen peroxide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-ascorbate peroxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 250249L-ascorbate peroxidase, cytosolic
PRO_0000055597

Sites

Active site421Proton acceptor
Metal binding1631Iron (heme axial ligand) Ref.4
Metal binding1641Potassium or calcium
Metal binding1801Potassium or calcium
Metal binding1821Potassium or calcium
Metal binding1851Potassium or calcium; via carbonyl oxygen
Metal binding1871Potassium or calcium
Site381Transition state stabilizer

Secondary structure

...................................... 250
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P48534 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 6F51006D0A13B42C

FASTA25027,193
        10         20         30         40         50         60 
MGKSYPTVSP DYQKAIEKAK RKLRGFIAEK KCAPLILRLA WHSAGTFDSK TKTGGPFGTI 

        70         80         90        100        110        120 
KHQAELAHGA NNGLDIAVRL LEPIKEQFPI VSYADFYQLA GVVAVEITGG PEVPFHPGRE 

       130        140        150        160        170        180 
DKPEPPPEGR LPDATKGSDH LRDVFGKAMG LSDQDIVALS GGHTIGAAHK ERSGFEGPWT 

       190        200        210        220        230        240 
SNPLIFDNSY FTELLTGEKD GLLQLPSDKA LLTDSVFRPL VEKYAADEDV FFADYAEAHL 

       250 
KLSELGFAEA 

« Hide

References

[1]"Molecular cloning and nucleotide sequence analysis of a cDNA encoding pea cytosolic ascorbate peroxidase."
Mittler R., Zilinskas B.A.
FEBS Lett. 289:257-259(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Little Marvel.
Tissue: Leaf.
[2]"Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase."
Mittler R., Zilinskas B.A.
J. Biol. Chem. 267:21802-21807(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
Strain: cv. Little Marvel.
[3]Erratum
Mittler R., Zilinskas B.A.
J. Biol. Chem. 268:4568-4568(1993)
[4]"Crystal structure of recombinant pea cytosolic ascorbate peroxidase."
Patterson W.R., Poulos T.L.
Biochemistry 34:4331-4341(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH HEME AND POTASSIUM IONS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M93051 Genomic DNA. Translation: AAA33645.1.
X62077 mRNA. Translation: CAA43992.1.
PIRA45116.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1APXX-ray2.20A/B/C/D2-250[»]
ProteinModelPortalP48534.
SMRP48534. Positions 2-250.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

PeroxiBase2462. PsAPx01.

Proteomic databases

ProMEXP48534.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP48534.

Family and domain databases

InterProIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMSSF48113. SSF48113. 1 hit.
PROSITEPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP48534.

Entry information

Entry nameAPX1_PEA
AccessionPrimary (citable) accession number: P48534
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references