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Protein

Serine/threonine-protein phosphatase PP-X isozyme 1

Gene

PPX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Manganese 1By similarity
Metal bindingi53 – 531Manganese 1By similarity
Metal bindingi79 – 791Manganese 1By similarity
Metal bindingi79 – 791Manganese 2By similarity
Metal bindingi111 – 1111Manganese 2By similarity
Active sitei112 – 1121Proton donorBy similarity
Metal bindingi161 – 1611Manganese 2By similarity
Metal bindingi236 – 2361Manganese 2By similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  • dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G26720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP-X isozyme 1 (EC:3.1.3.16)
Gene namesi
Name:PPX1
Synonyms:EP124, EP129
Ordered Locus Names:At4g26720
ORF Names:F10M23.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G26720.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: TAIR
  • plastid stroma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Serine/threonine-protein phosphatase PP-X isozyme 1PRO_0000058886Add
BLAST

Proteomic databases

PaxDbiP48529.
PRIDEiP48529.

Expressioni

Tissue specificityi

Ubiquitous, mostly expressed in root mersitems, flowers, and vascular tissues.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi14066. 1 interaction.
STRINGi3702.AT4G26720.1.

Structurei

3D structure databases

ProteinModelPortaliP48529.
SMRiP48529. Positions 3-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiP48529.
KOiK15423.
OMAiCELITEQ.
PhylomeDBiP48529.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLDRQIGQ LKRCEPLSES EVKALCLKAM EILVEESNVQ RVDAPVTLCG
60 70 80 90 100
DIHGQFYDMM ELFKVGGDCP KTNYLFMGDF VDRGYYSVET FLLLLALKVR
110 120 130 140 150
YPDRITLIRG NHESRQITQV YGFYDECLRK YGSSNVWRYC TDIFDYMSLS
160 170 180 190 200
AVVENKIFCV HGGLSPAIMT LDQIRTIDRK QEVPHDGAMC DLLWSDPEDI
210 220 230 240 250
VDGWGLSPRG AGFLFGGSVV TSFNHSNNID YIARAHQLVM EGYKWMFDSQ
260 270 280 290 300
IVTVWSAPNY CYRCGNVASI LELDENLNKE FRVFDAAQQD SRGPPAKKPA

PDYFL
Length:305
Mass (Da):34,738
Last modified:February 1, 1996 - v1
Checksum:i3FBBEADB2DA5B650
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22587 mRNA. Translation: CAA80302.1.
AF030289 Genomic DNA. Translation: AAB86418.1.
AL035440 Genomic DNA. Translation: CAB36518.1.
AL161565 Genomic DNA. Translation: CAB79527.1.
CP002687 Genomic DNA. Translation: AEE85245.1.
PIRiS42558.
RefSeqiNP_194402.1. NM_118806.5.
UniGeneiAt.106.

Genome annotation databases

EnsemblPlantsiAT4G26720.1; AT4G26720.1; AT4G26720.
GeneIDi828779.
KEGGiath:AT4G26720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22587 mRNA. Translation: CAA80302.1.
AF030289 Genomic DNA. Translation: AAB86418.1.
AL035440 Genomic DNA. Translation: CAB36518.1.
AL161565 Genomic DNA. Translation: CAB79527.1.
CP002687 Genomic DNA. Translation: AEE85245.1.
PIRiS42558.
RefSeqiNP_194402.1. NM_118806.5.
UniGeneiAt.106.

3D structure databases

ProteinModelPortaliP48529.
SMRiP48529. Positions 3-287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14066. 1 interaction.
STRINGi3702.AT4G26720.1.

Proteomic databases

PaxDbiP48529.
PRIDEiP48529.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G26720.1; AT4G26720.1; AT4G26720.
GeneIDi828779.
KEGGiath:AT4G26720.

Organism-specific databases

TAIRiAT4G26720.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiP48529.
KOiK15423.
OMAiCELITEQ.
PhylomeDBiP48529.

Enzyme and pathway databases

BioCyciARA:AT4G26720-MONOMER.

Miscellaneous databases

PROiP48529.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and molecular cloning of two homologues of protein phosphatase X from Arabidopsis thaliana."
    Perez-Callejon E., Casamayor A., Pujol G., Clua E., Ferrer A., Arino J.
    Plant Mol. Biol. 23:1177-1185(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  2. "The Arabidopsis thaliana PPX/PP4 phosphatases: molecular cloning and structural organization of the genes and immunolocalization of the proteins to plastids."
    Pujol G., Baskin T.I., Casamayor A., Cortadellas N., Ferrer A., Arino J.
    Plant Mol. Biol. 44:499-511(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPX1_ARATH
AccessioniPrimary (citable) accession number: P48529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.