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Protein

Serine/threonine-protein phosphatase PP-X isozyme 2

Gene

PPX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Manganese 1By similarity
Metal bindingi53 – 531Manganese 1By similarity
Metal bindingi79 – 791Manganese 1By similarity
Metal bindingi79 – 791Manganese 2By similarity
Metal bindingi111 – 1111Manganese 2By similarity
Active sitei112 – 1121Proton donorBy similarity
Metal bindingi161 – 1611Manganese 2By similarity
Metal bindingi236 – 2361Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G55260-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP-X isozyme 2 (EC:3.1.3.16)
Gene namesi
Name:PPX2
Ordered Locus Names:At5g55260
ORF Names:MCO15.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G55260.

Subcellular locationi

Plastid stroma 1 Publication

GO - Cellular componenti

  1. plastid stroma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Serine/threonine-protein phosphatase PP-X isozyme 2PRO_0000058887Add
BLAST

Proteomic databases

PaxDbiP48528.
PRIDEiP48528.

Expressioni

Tissue specificityi

Ubiquitous, mostly expressed in root mersitems, flowers, and vascular tissues.1 Publication

Gene expression databases

GenevestigatoriP48528.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G55260.1-P.

Structurei

3D structure databases

ProteinModelPortaliP48528.
SMRiP48528. Positions 3-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiP48528.
KOiK15423.
OMAiQFNYKND.
PhylomeDBiP48528.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLDKQIEQ LKRCEALKES EVKALCLKAM EILVEESNVQ RVDAPVTICG
60 70 80 90 100
DIHGQFYDMK ELFKVGGDCP KTNYLFLGDF VDRGFYSVET FLLLLALKVR
110 120 130 140 150
YPDRITLIRG NHESRQITQV YGFYDECLRK YGSVNVWRYC TDIFDYLSLS
160 170 180 190 200
ALVENKIFCV HGGLSPAIMT LDQIRAIDRK QEVPHDGAMC DLLWSDPEDI
210 220 230 240 250
VDGWGLSPRG AGFLFGGSVV TSFNHSNNID YICRAHQLVM EGYKWMFNSQ
260 270 280 290 300
IVTVWSAPNY CYRCGNVAAI LELDENLNKE FRVFDAAPQE SRGALAKKPA

PDYFL
Length:305
Mass (Da):34,726
Last modified:November 30, 2000 - v2
Checksum:i77DF2B662F3B1FB1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731N → H in CAA80312 (PubMed:8292782).Curated
Sequence conflicti226 – 2261S → L in CAA80312 (PubMed:8292782).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22596 mRNA. Translation: CAA80312.1.
AF030290 Genomic DNA. Translation: AAB86419.1.
AB010071 Genomic DNA. Translation: BAB08595.1.
CP002688 Genomic DNA. Translation: AED96607.1.
BT024716 mRNA. Translation: ABD59054.1.
PIRiS42559.
RefSeqiNP_200337.1. NM_124908.3.
UniGeneiAt.105.

Genome annotation databases

EnsemblPlantsiAT5G55260.1; AT5G55260.1; AT5G55260.
GeneIDi835619.
KEGGiath:AT5G55260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22596 mRNA. Translation: CAA80312.1.
AF030290 Genomic DNA. Translation: AAB86419.1.
AB010071 Genomic DNA. Translation: BAB08595.1.
CP002688 Genomic DNA. Translation: AED96607.1.
BT024716 mRNA. Translation: ABD59054.1.
PIRiS42559.
RefSeqiNP_200337.1. NM_124908.3.
UniGeneiAt.105.

3D structure databases

ProteinModelPortaliP48528.
SMRiP48528. Positions 3-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G55260.1-P.

Proteomic databases

PaxDbiP48528.
PRIDEiP48528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G55260.1; AT5G55260.1; AT5G55260.
GeneIDi835619.
KEGGiath:AT5G55260.

Organism-specific databases

TAIRiAT5G55260.

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000172696.
InParanoidiP48528.
KOiK15423.
OMAiQFNYKND.
PhylomeDBiP48528.

Enzyme and pathway databases

BioCyciARA:AT5G55260-MONOMER.

Miscellaneous databases

PROiP48528.

Gene expression databases

GenevestigatoriP48528.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and molecular cloning of two homologues of protein phosphatase X from Arabidopsis thaliana."
    Perez-Callejon E., Casamayor A., Pujol G., Clua E., Ferrer A., Arino J.
    Plant Mol. Biol. 23:1177-1185(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  2. "The Arabidopsis thaliana PPX/PP4 phosphatases: molecular cloning and structural organization of the genes and immunolocalization of the proteins to plastids."
    Pujol G., Baskin T.I., Casamayor A., Cortadellas N., Ferrer A., Arino J.
    Plant Mol. Biol. 44:499-511(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
    Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:41-54(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPX2_ARATH
AccessioniPrimary (citable) accession number: P48528
Secondary accession number(s): O22626, Q29Q40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: November 30, 2000
Last modified: January 6, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.