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P48528 (PPX2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase PP-X isozyme 2

EC=3.1.3.16
Gene names
Name:PPX2
Ordered Locus Names:At5g55260
ORF Names:MCO15.21
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Subcellular location

Plastid stroma Ref.2.

Tissue specificity

Ubiquitous, mostly expressed in root mersitems, flowers, and vascular tissues. Ref.2

Sequence similarities

Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

Ontologies

Keywords
   Cellular componentPlastid
   LigandManganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from sequence or structural similarity Ref.1. Source: GOC

   Cellular_componentplastid stroma

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine phosphatase activity

Inferred from sequence or structural similarity Ref.1. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305Serine/threonine-protein phosphatase PP-X isozyme 2
PRO_0000058887

Sites

Active site1121Proton donor By similarity
Metal binding511Manganese 1 By similarity
Metal binding531Manganese 1 By similarity
Metal binding791Manganese 1 By similarity
Metal binding791Manganese 2 By similarity
Metal binding1111Manganese 2 By similarity
Metal binding1611Manganese 2 By similarity
Metal binding2361Manganese 2 By similarity

Experimental info

Sequence conflict731N → H in CAA80312. Ref.1
Sequence conflict2261S → L in CAA80312. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48528 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 77DF2B662F3B1FB1

FASTA30534,726
        10         20         30         40         50         60 
MSDLDKQIEQ LKRCEALKES EVKALCLKAM EILVEESNVQ RVDAPVTICG DIHGQFYDMK 

        70         80         90        100        110        120 
ELFKVGGDCP KTNYLFLGDF VDRGFYSVET FLLLLALKVR YPDRITLIRG NHESRQITQV 

       130        140        150        160        170        180 
YGFYDECLRK YGSVNVWRYC TDIFDYLSLS ALVENKIFCV HGGLSPAIMT LDQIRAIDRK 

       190        200        210        220        230        240 
QEVPHDGAMC DLLWSDPEDI VDGWGLSPRG AGFLFGGSVV TSFNHSNNID YICRAHQLVM 

       250        260        270        280        290        300 
EGYKWMFNSQ IVTVWSAPNY CYRCGNVAAI LELDENLNKE FRVFDAAPQE SRGALAKKPA 


PDYFL 

« Hide

References

« Hide 'large scale' references
[1]"Identification and molecular cloning of two homologues of protein phosphatase X from Arabidopsis thaliana."
Perez-Callejon E., Casamayor A., Pujol G., Clua E., Ferrer A., Arino J.
Plant Mol. Biol. 23:1177-1185(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.
[2]"The Arabidopsis thaliana PPX/PP4 phosphatases: molecular cloning and structural organization of the genes and immunolocalization of the proteins to plastids."
Pujol G., Baskin T.I., Casamayor A., Cortadellas N., Ferrer A., Arino J.
Plant Mol. Biol. 44:499-511(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Arabidopsis ORF clones."
Shinn P., Chen H., Kim C.J., Ecker J.R.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Arabidopsis PPP family of serine/threonine phosphatases."
Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z22596 mRNA. Translation: CAA80312.1.
AF030290 Genomic DNA. Translation: AAB86419.1.
AB010071 Genomic DNA. Translation: BAB08595.1.
CP002688 Genomic DNA. Translation: AED96607.1.
BT024716 mRNA. Translation: ABD59054.1.
PIRS42559.
RefSeqNP_200337.1. NM_124908.3.
UniGeneAt.105.

3D structure databases

ProteinModelPortalP48528.
SMRP48528. Positions 3-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G55260.1-P.

Proteomic databases

PaxDbP48528.
PRIDEP48528.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G55260.1; AT5G55260.1; AT5G55260.
GeneID835619.
KEGGath:AT5G55260.

Organism-specific databases

TAIRAT5G55260.

Phylogenomic databases

eggNOGCOG0639.
HOGENOMHOG000172696.
InParanoidP48528.
KOK15423.
OMAVFNHRND.
PhylomeDBP48528.

Enzyme and pathway databases

BioCycARA:AT5G55260-MONOMER.

Gene expression databases

GenevestigatorP48528.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP48528.

Entry information

Entry namePPX2_ARATH
AccessionPrimary (citable) accession number: P48528
Secondary accession number(s): O22626, Q29Q40
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: December 1, 2000
Last modified: June 11, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names