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P48528

- PPX2_ARATH

UniProt

P48528 - PPX2_ARATH

Protein

Serine/threonine-protein phosphatase PP-X isozyme 2

Gene

PPX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi51 – 511Manganese 1By similarity
    Metal bindingi53 – 531Manganese 1By similarity
    Metal bindingi79 – 791Manganese 1By similarity
    Metal bindingi79 – 791Manganese 2By similarity
    Metal bindingi111 – 1111Manganese 2By similarity
    Active sitei112 – 1121Proton donorBy similarity
    Metal bindingi161 – 1611Manganese 2By similarity
    Metal bindingi236 – 2361Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G55260-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP-X isozyme 2 (EC:3.1.3.16)
    Gene namesi
    Name:PPX2
    Ordered Locus Names:At5g55260
    ORF Names:MCO15.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G55260.

    Subcellular locationi

    Plastid stroma 1 Publication

    GO - Cellular componenti

    1. plastid stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 305305Serine/threonine-protein phosphatase PP-X isozyme 2PRO_0000058887Add
    BLAST

    Proteomic databases

    PaxDbiP48528.
    PRIDEiP48528.

    Expressioni

    Tissue specificityi

    Ubiquitous, mostly expressed in root mersitems, flowers, and vascular tissues.1 Publication

    Gene expression databases

    GenevestigatoriP48528.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G55260.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP48528.
    SMRiP48528. Positions 3-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172696.
    InParanoidiP48528.
    KOiK15423.
    OMAiVFNHRND.
    PhylomeDBiP48528.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48528-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDLDKQIEQ LKRCEALKES EVKALCLKAM EILVEESNVQ RVDAPVTICG    50
    DIHGQFYDMK ELFKVGGDCP KTNYLFLGDF VDRGFYSVET FLLLLALKVR 100
    YPDRITLIRG NHESRQITQV YGFYDECLRK YGSVNVWRYC TDIFDYLSLS 150
    ALVENKIFCV HGGLSPAIMT LDQIRAIDRK QEVPHDGAMC DLLWSDPEDI 200
    VDGWGLSPRG AGFLFGGSVV TSFNHSNNID YICRAHQLVM EGYKWMFNSQ 250
    IVTVWSAPNY CYRCGNVAAI LELDENLNKE FRVFDAAPQE SRGALAKKPA 300
    PDYFL 305
    Length:305
    Mass (Da):34,726
    Last modified:December 1, 2000 - v2
    Checksum:i77DF2B662F3B1FB1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731N → H in CAA80312. (PubMed:8292782)Curated
    Sequence conflicti226 – 2261S → L in CAA80312. (PubMed:8292782)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22596 mRNA. Translation: CAA80312.1.
    AF030290 Genomic DNA. Translation: AAB86419.1.
    AB010071 Genomic DNA. Translation: BAB08595.1.
    CP002688 Genomic DNA. Translation: AED96607.1.
    BT024716 mRNA. Translation: ABD59054.1.
    PIRiS42559.
    RefSeqiNP_200337.1. NM_124908.3.
    UniGeneiAt.105.

    Genome annotation databases

    EnsemblPlantsiAT5G55260.1; AT5G55260.1; AT5G55260.
    GeneIDi835619.
    KEGGiath:AT5G55260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22596 mRNA. Translation: CAA80312.1 .
    AF030290 Genomic DNA. Translation: AAB86419.1 .
    AB010071 Genomic DNA. Translation: BAB08595.1 .
    CP002688 Genomic DNA. Translation: AED96607.1 .
    BT024716 mRNA. Translation: ABD59054.1 .
    PIRi S42559.
    RefSeqi NP_200337.1. NM_124908.3.
    UniGenei At.105.

    3D structure databases

    ProteinModelPortali P48528.
    SMRi P48528. Positions 3-289.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G55260.1-P.

    Proteomic databases

    PaxDbi P48528.
    PRIDEi P48528.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G55260.1 ; AT5G55260.1 ; AT5G55260 .
    GeneIDi 835619.
    KEGGi ath:AT5G55260.

    Organism-specific databases

    TAIRi AT5G55260.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172696.
    InParanoidi P48528.
    KOi K15423.
    OMAi VFNHRND.
    PhylomeDBi P48528.

    Enzyme and pathway databases

    BioCyci ARA:AT5G55260-MONOMER.

    Miscellaneous databases

    PROi P48528.

    Gene expression databases

    Genevestigatori P48528.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and molecular cloning of two homologues of protein phosphatase X from Arabidopsis thaliana."
      Perez-Callejon E., Casamayor A., Pujol G., Clua E., Ferrer A., Arino J.
      Plant Mol. Biol. 23:1177-1185(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Leaf.
    2. "The Arabidopsis thaliana PPX/PP4 phosphatases: molecular cloning and structural organization of the genes and immunolocalization of the proteins to plastids."
      Pujol G., Baskin T.I., Casamayor A., Cortadellas N., Ferrer A., Arino J.
      Plant Mol. Biol. 44:499-511(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
      Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Ecker J.R.
      Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Arabidopsis PPP family of serine/threonine phosphatases."
      Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
      Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPPX2_ARATH
    AccessioniPrimary (citable) accession number: P48528
    Secondary accession number(s): O22626, Q29Q40
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3