Reviewed, UniProtKB/Swiss-Prot P48527 (SYYM_YEAST)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase, mitochondrial
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	MSY1
Ordered Locus Names:	YPL097W
ORF Names:	LPG11W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).
Subcellular location	Mitochondrion matrix. Ref.3 Ref.5 Ref.6
Miscellaneous	Present with 996 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Traceable author statement. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct tyrosine-tRNA ligase activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-18851,EBI-18851

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 492		Tyrosyl-tRNA synthetase, mitochondrial		PRO_0000035835
Regions
	Motif	94 – 103	10	"HIGH" region
	Motif	303 – 307	5	"KMSKS" region
Sites
	Binding site	306	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P48527-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 2159116E1CB5ED2E	FASTA	492	55,287
	10 20 30 40 50 60 MLELRSCSNL VNSSRRLVPL VTYSGLSAIT LPKSRFYSQP SALEVQGTSD SRSDNILDEL 70 80 90 100 110 120 KQRGLVSQVS QPESFLRTKL NGNDKIKLYC GVDPTAQSLH LGNLVPLMVL LHFYVKGHDI 130 140 150 160 170 180 VTVIGGATGK VGDPSGRKTE RDVMENDIRQ SNVASISQQL QRFFKNGLEY YRNRCALTED 190 200 210 220 230 240 VPSGKYTPRN NFNWWKDIKM LDFLADFGRH IRVQSMLARD SISSRLQTKN GLGFNEFTYQ 250 260 270 280 290 300 VLQAYDFYHL YKEENVTIQV GGNDQWGNIT AGIDLINRIQ PIKNKGLPFG ITVPLLTTAT 310 320 330 340 350 360 GEKFGKSAGN AVFIDPSINT AYDVYQFFYN TLDADVPKFL KIFTFLNSSE IKKIVETHIK 370 380 390 400 410 420 SPSLRYGQTL LAKEVTDMLY GVGSGSDSEA LSNIIFGRYD GTLSAAKLVD LCKKARILQY 430 440 450 460 470 480 ADREIDLIKL ICKLVNCSVS EARRKLSQGS VYLHHSKSKV NENISNLAPF LIDDRVLILR 490 IGKQKCFIIE MR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nuclear gene MSY1 of Saccharomyces cerevisiae codes for mitochondrial tyrosyl-tRNA synthetase." Hill J.E., Tzagoloff A.A. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed: 16823961] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42333 Genomic DNA. Translation: AAA67122.1. U43281 Genomic DNA. Translation: AAB68202.1.
PIR	S59733.
RefSeq	NP_015228.1.
3D structure databases
HSSP	HSSP built from PDB template 1TYC based on UniProtKB P00952.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3826N.
IntAct	P48527. 2 interactions.
STRING	P48527.
Genome annotation databases
Ensembl	YPL097W; YPL097W; YPL097W; Saccharomyces cerevisiae. [Genome view]
GeneID	856007.
GenomeReviews	Gene locus YPL097W in contig U00094_GR.
KEGG	sce:YPL097W.
NMPDR	fig|4932.3.peg.6360.
Organism-specific databases
CYGD	YPL097w.
SGD	S000006018. MSY1.
Phylogenomic databases
HOGENOM	P48527.
OMA	MLARDSI.
Enzyme and pathway databases
BRENDA	6.1.1.1. 250.
Gene expression databases
ArrayExpress	P48527.
Genevestigator	P48527.
GermOnline	YPL097W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	980887.

Entry information

Entry name

SYYM_YEAST

Accession

Primary (citable) accession number: P48527

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)