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Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

ISM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei671 – 6711ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • isoleucyl-tRNA aminoacylation Source: SGD
  • mitochondrial isoleucyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33954-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ISM1
Ordered Locus Names:YPL040C
ORF Names:P7102.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPL040c.
EuPathDBiFungiDB:YPL040C.
SGDiS000005961. ISM1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Isoleucine--tRNA ligase, mitochondrialPRO_0000098605Add
BLAST

Proteomic databases

MaxQBiP48526.
PaxDbiP48526.
PeptideAtlasiP48526.

Interactioni

Protein-protein interaction databases

BioGridi36139. 16 interactions.
DIPiDIP-4034N.
IntActiP48526. 2 interactions.
MINTiMINT-514793.
STRINGi4932.YPL040C.

Structurei

3D structure databases

ProteinModelPortaliP48526.
SMRiP48526. Positions 49-458, 512-696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi94 – 10411"HIGH" regionAdd
BLAST
Motifi668 – 6725"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074910.
HOGENOMiHOG000246402.
InParanoidiP48526.
KOiK01870.
OMAiSYLGEHV.
OrthoDBiEOG7JMGNT.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSRLVPQH IFSIISKRYL AKHAYQKTLN LPKTKFPNRS NLEITLRELI
60 70 80 90 100
PKSSQLVYKE QLRDFFEEFS KLNTTDEKLE FIKEKLFILH DGPPYANGEL
110 120 130 140 150
HLGHALNKIL KDIINRYQLS QGKYIFYKPG WDCHGLPIEI KALKDLSAQQ
160 170 180 190 200
IESISPLKIR SMALKHAQKA IKRQRETFQH FAILTDWETP YLTMDKDYEI
210 220 230 240 250
NQLNIFKEMY ERGLIKRQNK PVYWGTETRT ALAEGELEYN ENHKSIAAYV
260 270 280 290 300
KFPLEKKSQM DLCKKLGITN NLPIYCLIWT STPWTLLSNR AICFNQDFSY
310 320 330 340 350
SLLRLNSELI LVETGSIDKL GLTTNSFETI KQFQGTHLNG LYYQNLLVDD
360 370 380 390 400
KVGRPLLHGA HVTSGTGTGL VHTAPGHGQD DYLIGIQNGL EIYSPVDHQG
410 420 430 440 450
RYQLNELPQS VRSIVRDEGD LTKGRQVLDA ETAKIILCKL SDLNLLYKSH
460 470 480 490 500
EYTHSYPYDW RSKKPVIIRA TPQWFADLHD VKNLALESIS RVKFCPKRGY
510 520 530 540 550
SRLSSFMKSR NEWCISRQRS WGIPILSFYK KSEPDSVLMN SEILAHAIEK
560 570 580 590 600
IKQKGINAWF NDKDNDMKEW LPEKYHDVAH EYCRSQDTMD VWFDSGSSWS
610 620 630 640 650
VIKDFYEKSL KLSKLPSPLY QVCLEGSDQH RGWFQSSLLT KVASSNVPVA
660 670 680 690 700
PYEEVITHGF TLDENGLKMS KSVGNTISPE AIIRGDENLG LPALGVDGLR
710 720 730 740 750
YLIAQSNFTT DIVAGPTVMK HVGEALKKVR LTFRYLLSNL QKSQDFNLLP
760 770 780 790 800
IEQLRRVDQY TLYKINELLE TTREHYQKYN FSKVLITLQY HLNNELSAFY
810 820 830 840 850
FDISKDILYS NQISSLARRQ VQTTLVHILN AYRAILAPIL PVMVQEVWKY
860 870 880 890 900
IPEGWLQGQE HIDINPMRGK WPFLDSNTEI VTSFENFELK ILKQFQEEFK
910 920 930 940 950
RLSLEEGVTK TTHSHVTIFT KHHLPFSSDE LCDILQSSAV DILQMDDNNN
960 970 980 990 1000
SLPTIELGSG INVQILVERS KRHNCPRCWK ANSAEEDKLC DRCKEAVDHL

MS
Length:1,002
Mass (Da):115,794
Last modified:October 1, 1996 - v2
Checksum:i2785B9DED636529E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti437 – 4371L → P in AAA64890 (Ref. 1) Curated
Sequence conflicti672 – 6721S → L in AAA64890 (Ref. 1) Curated
Sequence conflicti806 – 8061D → G in AAA64890 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38957 Genomic DNA. Translation: AAA64890.1.
U44030 Genomic DNA. Translation: AAB68180.1.
BK006949 Genomic DNA. Translation: DAA11389.1.
PIRiS62035.
RefSeqiNP_015285.1. NM_001183854.1.

Genome annotation databases

EnsemblFungiiYPL040C; YPL040C; YPL040C.
GeneIDi856067.
KEGGisce:YPL040C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38957 Genomic DNA. Translation: AAA64890.1.
U44030 Genomic DNA. Translation: AAB68180.1.
BK006949 Genomic DNA. Translation: DAA11389.1.
PIRiS62035.
RefSeqiNP_015285.1. NM_001183854.1.

3D structure databases

ProteinModelPortaliP48526.
SMRiP48526. Positions 49-458, 512-696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36139. 16 interactions.
DIPiDIP-4034N.
IntActiP48526. 2 interactions.
MINTiMINT-514793.
STRINGi4932.YPL040C.

Proteomic databases

MaxQBiP48526.
PaxDbiP48526.
PeptideAtlasiP48526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL040C; YPL040C; YPL040C.
GeneIDi856067.
KEGGisce:YPL040C.

Organism-specific databases

CYGDiYPL040c.
EuPathDBiFungiDB:YPL040C.
SGDiS000005961. ISM1.

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074910.
HOGENOMiHOG000246402.
InParanoidiP48526.
KOiK01870.
OMAiSYLGEHV.
OrthoDBiEOG7JMGNT.

Enzyme and pathway databases

BioCyciYEAST:G3O-33954-MONOMER.

Miscellaneous databases

NextBioi981053.
PROiP48526.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Tzagoloff A.A., Shtanko A.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24657 / D273-10B.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYIM_YEAST
AccessioniPrimary (citable) accession number: P48526
Secondary accession number(s): D6W3X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.