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P48526

- SYIM_YEAST

UniProt

P48526 - SYIM_YEAST

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Protein

Isoleucine--tRNA ligase, mitochondrial

Gene
ISM1, YPL040C, P7102.10
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei671 – 6711ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: SGD

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: SGD
  2. mitochondrial isoleucyl-tRNA aminoacylation Source: SGD
  3. mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33954-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ISM1
Ordered Locus Names:YPL040C
ORF Names:P7102.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL040c.
SGDiS000005961. ISM1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Isoleucine--tRNA ligase, mitochondrialPRO_0000098605Add
BLAST

Proteomic databases

MaxQBiP48526.
PaxDbiP48526.
PeptideAtlasiP48526.

Expressioni

Gene expression databases

GenevestigatoriP48526.

Interactioni

Protein-protein interaction databases

BioGridi36139. 16 interactions.
DIPiDIP-4034N.
IntActiP48526. 2 interactions.
MINTiMINT-514793.
STRINGi4932.YPL040C.

Structurei

3D structure databases

ProteinModelPortaliP48526.
SMRiP48526. Positions 49-458, 512-696.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi94 – 10411"HIGH" regionAdd
BLAST
Motifi668 – 6725"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074910.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEYPEGHI.
OrthoDBiEOG7JMGNT.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P48526-1 [UniParc]FASTAAdd to Basket

« Hide

MKRSRLVPQH IFSIISKRYL AKHAYQKTLN LPKTKFPNRS NLEITLRELI     50
PKSSQLVYKE QLRDFFEEFS KLNTTDEKLE FIKEKLFILH DGPPYANGEL 100
HLGHALNKIL KDIINRYQLS QGKYIFYKPG WDCHGLPIEI KALKDLSAQQ 150
IESISPLKIR SMALKHAQKA IKRQRETFQH FAILTDWETP YLTMDKDYEI 200
NQLNIFKEMY ERGLIKRQNK PVYWGTETRT ALAEGELEYN ENHKSIAAYV 250
KFPLEKKSQM DLCKKLGITN NLPIYCLIWT STPWTLLSNR AICFNQDFSY 300
SLLRLNSELI LVETGSIDKL GLTTNSFETI KQFQGTHLNG LYYQNLLVDD 350
KVGRPLLHGA HVTSGTGTGL VHTAPGHGQD DYLIGIQNGL EIYSPVDHQG 400
RYQLNELPQS VRSIVRDEGD LTKGRQVLDA ETAKIILCKL SDLNLLYKSH 450
EYTHSYPYDW RSKKPVIIRA TPQWFADLHD VKNLALESIS RVKFCPKRGY 500
SRLSSFMKSR NEWCISRQRS WGIPILSFYK KSEPDSVLMN SEILAHAIEK 550
IKQKGINAWF NDKDNDMKEW LPEKYHDVAH EYCRSQDTMD VWFDSGSSWS 600
VIKDFYEKSL KLSKLPSPLY QVCLEGSDQH RGWFQSSLLT KVASSNVPVA 650
PYEEVITHGF TLDENGLKMS KSVGNTISPE AIIRGDENLG LPALGVDGLR 700
YLIAQSNFTT DIVAGPTVMK HVGEALKKVR LTFRYLLSNL QKSQDFNLLP 750
IEQLRRVDQY TLYKINELLE TTREHYQKYN FSKVLITLQY HLNNELSAFY 800
FDISKDILYS NQISSLARRQ VQTTLVHILN AYRAILAPIL PVMVQEVWKY 850
IPEGWLQGQE HIDINPMRGK WPFLDSNTEI VTSFENFELK ILKQFQEEFK 900
RLSLEEGVTK TTHSHVTIFT KHHLPFSSDE LCDILQSSAV DILQMDDNNN 950
SLPTIELGSG INVQILVERS KRHNCPRCWK ANSAEEDKLC DRCKEAVDHL 1000
MS 1002
Length:1,002
Mass (Da):115,794
Last modified:October 1, 1996 - v2
Checksum:i2785B9DED636529E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti437 – 4371L → P in AAA64890. 1 Publication
Sequence conflicti672 – 6721S → L in AAA64890. 1 Publication
Sequence conflicti806 – 8061D → G in AAA64890. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L38957 Genomic DNA. Translation: AAA64890.1.
U44030 Genomic DNA. Translation: AAB68180.1.
BK006949 Genomic DNA. Translation: DAA11389.1.
PIRiS62035.
RefSeqiNP_015285.1. NM_001183854.1.

Genome annotation databases

EnsemblFungiiYPL040C; YPL040C; YPL040C.
GeneIDi856067.
KEGGisce:YPL040C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L38957 Genomic DNA. Translation: AAA64890.1 .
U44030 Genomic DNA. Translation: AAB68180.1 .
BK006949 Genomic DNA. Translation: DAA11389.1 .
PIRi S62035.
RefSeqi NP_015285.1. NM_001183854.1.

3D structure databases

ProteinModelPortali P48526.
SMRi P48526. Positions 49-458, 512-696.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36139. 16 interactions.
DIPi DIP-4034N.
IntActi P48526. 2 interactions.
MINTi MINT-514793.
STRINGi 4932.YPL040C.

Proteomic databases

MaxQBi P48526.
PaxDbi P48526.
PeptideAtlasi P48526.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL040C ; YPL040C ; YPL040C .
GeneIDi 856067.
KEGGi sce:YPL040C.

Organism-specific databases

CYGDi YPL040c.
SGDi S000005961. ISM1.

Phylogenomic databases

eggNOGi COG0060.
GeneTreei ENSGT00550000074910.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EYPEGHI.
OrthoDBi EOG7JMGNT.

Enzyme and pathway databases

BioCyci YEAST:G3O-33954-MONOMER.

Miscellaneous databases

NextBioi 981053.
PROi P48526.

Gene expression databases

Genevestigatori P48526.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Tzagoloff A.A., Shtanko A.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24657 / D273-10B.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYIM_YEAST
AccessioniPrimary (citable) accession number: P48526
Secondary accession number(s): D6W3X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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