Reviewed, UniProtKB/Swiss-Prot P48526 (SYIM_YEAST)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase, mitochondrial
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ISM1
Ordered Locus Names:	YPL040C
ORF Names:	P7102.10

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1002 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 1270 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial isoleucyl-tRNA aminoacylation Inferred by curator. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-18725,EBI-24597
BUB2	P26448	1	EBI-18725,EBI-3824

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1002	1002	Isoleucyl-tRNA synthetase, mitochondrial		PRO_0000098605
Regions
	Motif	94 – 104	11	"HIGH" region
	Motif	668 – 672	5	"KMSKS" region
Sites
	Binding site	671	1	ATP By similarity
Experimental info
	Sequence conflict	437	1	L → P in AAA64890. Ref.1
	Sequence conflict	672	1	S → L in AAA64890. Ref.1
	Sequence conflict	806	1	D → G in AAA64890. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48526-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 2785B9DED636529E	FASTA	1,002	115,794
	10 20 30 40 50 60 MKRSRLVPQH IFSIISKRYL AKHAYQKTLN LPKTKFPNRS NLEITLRELI PKSSQLVYKE 70 80 90 100 110 120 QLRDFFEEFS KLNTTDEKLE FIKEKLFILH DGPPYANGEL HLGHALNKIL KDIINRYQLS 130 140 150 160 170 180 QGKYIFYKPG WDCHGLPIEI KALKDLSAQQ IESISPLKIR SMALKHAQKA IKRQRETFQH 190 200 210 220 230 240 FAILTDWETP YLTMDKDYEI NQLNIFKEMY ERGLIKRQNK PVYWGTETRT ALAEGELEYN 250 260 270 280 290 300 ENHKSIAAYV KFPLEKKSQM DLCKKLGITN NLPIYCLIWT STPWTLLSNR AICFNQDFSY 310 320 330 340 350 360 SLLRLNSELI LVETGSIDKL GLTTNSFETI KQFQGTHLNG LYYQNLLVDD KVGRPLLHGA 370 380 390 400 410 420 HVTSGTGTGL VHTAPGHGQD DYLIGIQNGL EIYSPVDHQG RYQLNELPQS VRSIVRDEGD 430 440 450 460 470 480 LTKGRQVLDA ETAKIILCKL SDLNLLYKSH EYTHSYPYDW RSKKPVIIRA TPQWFADLHD 490 500 510 520 530 540 VKNLALESIS RVKFCPKRGY SRLSSFMKSR NEWCISRQRS WGIPILSFYK KSEPDSVLMN 550 560 570 580 590 600 SEILAHAIEK IKQKGINAWF NDKDNDMKEW LPEKYHDVAH EYCRSQDTMD VWFDSGSSWS 610 620 630 640 650 660 VIKDFYEKSL KLSKLPSPLY QVCLEGSDQH RGWFQSSLLT KVASSNVPVA PYEEVITHGF 670 680 690 700 710 720 TLDENGLKMS KSVGNTISPE AIIRGDENLG LPALGVDGLR YLIAQSNFTT DIVAGPTVMK 730 740 750 760 770 780 HVGEALKKVR LTFRYLLSNL QKSQDFNLLP IEQLRRVDQY TLYKINELLE TTREHYQKYN 790 800 810 820 830 840 FSKVLITLQY HLNNELSAFY FDISKDILYS NQISSLARRQ VQTTLVHILN AYRAILAPIL 850 860 870 880 890 900 PVMVQEVWKY IPEGWLQGQE HIDINPMRGK WPFLDSNTEI VTSFENFELK ILKQFQEEFK 910 920 930 940 950 960 RLSLEEGVTK TTHSHVTIFT KHHLPFSSDE LCDILQSSAV DILQMDDNNN SLPTIELGSG 970 980 990 1000 INVQILVERS KRHNCPRCWK ANSAEEDKLC DRCKEAVDHL MS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Tzagoloff A.A., Shtanko A. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L38957 Genomic DNA. Translation: AAA64890.1. U44030 Genomic DNA. Translation: AAB68180.1.
PIR	S62035.
RefSeq	NP_015285.1.
3D structure databases
HSSP	HSSP built from PDB template 1FFY based on UniProtKB P41972.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4034N.
IntAct	P48526. 5 interactions.
STRING	P48526.
Proteomic databases
PeptideAtlas	P48526.
Genome annotation databases
Ensembl	YPL040C; YPL040C; YPL040C; Saccharomyces cerevisiae. [Genome view]
GeneID	856067.
GenomeReviews	Gene locus YPL040C in contig U00094_GR.
KEGG	sce:YPL040C.
NMPDR	fig|4932.3.peg.6418.
Organism-specific databases
CYGD	YPL040c.
SGD	S000005961. ISM1.
Phylogenomic databases
HOGENOM	P48526.
OMA	FPMRGNL.
Enzyme and pathway databases
BRENDA	6.1.1.5. 250.
Gene expression databases
ArrayExpress	P48526.
Genevestigator	P48526.
GermOnline	YPL040C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981053.

Entry information

Entry name

SYIM_YEAST

Accession

Primary (citable) accession number: P48526

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)