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P48526

- SYIM_YEAST

UniProt

P48526 - SYIM_YEAST

Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

ISM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei671 – 6711ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: SGD

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: SGD
    2. mitochondrial isoleucyl-tRNA aminoacylation Source: SGD
    3. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33954-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:ISM1
    Ordered Locus Names:YPL040C
    ORF Names:P7102.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL040c.
    SGDiS000005961. ISM1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10021002Isoleucine--tRNA ligase, mitochondrialPRO_0000098605Add
    BLAST

    Proteomic databases

    MaxQBiP48526.
    PaxDbiP48526.
    PeptideAtlasiP48526.

    Expressioni

    Gene expression databases

    GenevestigatoriP48526.

    Interactioni

    Protein-protein interaction databases

    BioGridi36139. 16 interactions.
    DIPiDIP-4034N.
    IntActiP48526. 2 interactions.
    MINTiMINT-514793.
    STRINGi4932.YPL040C.

    Structurei

    3D structure databases

    ProteinModelPortaliP48526.
    SMRiP48526. Positions 49-458, 512-696.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi94 – 10411"HIGH" regionAdd
    BLAST
    Motifi668 – 6725"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.
    GeneTreeiENSGT00550000074910.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiEYPEGHI.
    OrthoDBiEOG7JMGNT.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P48526-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRSRLVPQH IFSIISKRYL AKHAYQKTLN LPKTKFPNRS NLEITLRELI     50
    PKSSQLVYKE QLRDFFEEFS KLNTTDEKLE FIKEKLFILH DGPPYANGEL 100
    HLGHALNKIL KDIINRYQLS QGKYIFYKPG WDCHGLPIEI KALKDLSAQQ 150
    IESISPLKIR SMALKHAQKA IKRQRETFQH FAILTDWETP YLTMDKDYEI 200
    NQLNIFKEMY ERGLIKRQNK PVYWGTETRT ALAEGELEYN ENHKSIAAYV 250
    KFPLEKKSQM DLCKKLGITN NLPIYCLIWT STPWTLLSNR AICFNQDFSY 300
    SLLRLNSELI LVETGSIDKL GLTTNSFETI KQFQGTHLNG LYYQNLLVDD 350
    KVGRPLLHGA HVTSGTGTGL VHTAPGHGQD DYLIGIQNGL EIYSPVDHQG 400
    RYQLNELPQS VRSIVRDEGD LTKGRQVLDA ETAKIILCKL SDLNLLYKSH 450
    EYTHSYPYDW RSKKPVIIRA TPQWFADLHD VKNLALESIS RVKFCPKRGY 500
    SRLSSFMKSR NEWCISRQRS WGIPILSFYK KSEPDSVLMN SEILAHAIEK 550
    IKQKGINAWF NDKDNDMKEW LPEKYHDVAH EYCRSQDTMD VWFDSGSSWS 600
    VIKDFYEKSL KLSKLPSPLY QVCLEGSDQH RGWFQSSLLT KVASSNVPVA 650
    PYEEVITHGF TLDENGLKMS KSVGNTISPE AIIRGDENLG LPALGVDGLR 700
    YLIAQSNFTT DIVAGPTVMK HVGEALKKVR LTFRYLLSNL QKSQDFNLLP 750
    IEQLRRVDQY TLYKINELLE TTREHYQKYN FSKVLITLQY HLNNELSAFY 800
    FDISKDILYS NQISSLARRQ VQTTLVHILN AYRAILAPIL PVMVQEVWKY 850
    IPEGWLQGQE HIDINPMRGK WPFLDSNTEI VTSFENFELK ILKQFQEEFK 900
    RLSLEEGVTK TTHSHVTIFT KHHLPFSSDE LCDILQSSAV DILQMDDNNN 950
    SLPTIELGSG INVQILVERS KRHNCPRCWK ANSAEEDKLC DRCKEAVDHL 1000
    MS 1002
    Length:1,002
    Mass (Da):115,794
    Last modified:October 1, 1996 - v2
    Checksum:i2785B9DED636529E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti437 – 4371L → P in AAA64890. 1 PublicationCurated
    Sequence conflicti672 – 6721S → L in AAA64890. 1 PublicationCurated
    Sequence conflicti806 – 8061D → G in AAA64890. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38957 Genomic DNA. Translation: AAA64890.1.
    U44030 Genomic DNA. Translation: AAB68180.1.
    BK006949 Genomic DNA. Translation: DAA11389.1.
    PIRiS62035.
    RefSeqiNP_015285.1. NM_001183854.1.

    Genome annotation databases

    EnsemblFungiiYPL040C; YPL040C; YPL040C.
    GeneIDi856067.
    KEGGisce:YPL040C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38957 Genomic DNA. Translation: AAA64890.1 .
    U44030 Genomic DNA. Translation: AAB68180.1 .
    BK006949 Genomic DNA. Translation: DAA11389.1 .
    PIRi S62035.
    RefSeqi NP_015285.1. NM_001183854.1.

    3D structure databases

    ProteinModelPortali P48526.
    SMRi P48526. Positions 49-458, 512-696.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36139. 16 interactions.
    DIPi DIP-4034N.
    IntActi P48526. 2 interactions.
    MINTi MINT-514793.
    STRINGi 4932.YPL040C.

    Proteomic databases

    MaxQBi P48526.
    PaxDbi P48526.
    PeptideAtlasi P48526.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL040C ; YPL040C ; YPL040C .
    GeneIDi 856067.
    KEGGi sce:YPL040C.

    Organism-specific databases

    CYGDi YPL040c.
    SGDi S000005961. ISM1.

    Phylogenomic databases

    eggNOGi COG0060.
    GeneTreei ENSGT00550000074910.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi EYPEGHI.
    OrthoDBi EOG7JMGNT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33954-MONOMER.

    Miscellaneous databases

    NextBioi 981053.
    PROi P48526.

    Gene expression databases

    Genevestigatori P48526.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Tzagoloff A.A., Shtanko A.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 24657 / D273-10B.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYIM_YEAST
    AccessioniPrimary (citable) accession number: P48526
    Secondary accession number(s): D6W3X3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1270 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3