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P48526 (SYIM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase, mitochondrial

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ISM1
Ordered Locus Names:YPL040C
ORF Names:P7102.10
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1002 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Subcellular location

Mitochondrion matrix.

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10021002Isoleucine--tRNA ligase, mitochondrial
PRO_0000098605

Regions

Motif94 – 10411"HIGH" region
Motif668 – 6725"KMSKS" region

Sites

Binding site6711ATP By similarity

Experimental info

Sequence conflict4371L → P in AAA64890. Ref.1
Sequence conflict6721S → L in AAA64890. Ref.1
Sequence conflict8061D → G in AAA64890. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P48526 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 2785B9DED636529E

FASTA1,002115,794
        10         20         30         40         50         60 
MKRSRLVPQH IFSIISKRYL AKHAYQKTLN LPKTKFPNRS NLEITLRELI PKSSQLVYKE 

        70         80         90        100        110        120 
QLRDFFEEFS KLNTTDEKLE FIKEKLFILH DGPPYANGEL HLGHALNKIL KDIINRYQLS 

       130        140        150        160        170        180 
QGKYIFYKPG WDCHGLPIEI KALKDLSAQQ IESISPLKIR SMALKHAQKA IKRQRETFQH 

       190        200        210        220        230        240 
FAILTDWETP YLTMDKDYEI NQLNIFKEMY ERGLIKRQNK PVYWGTETRT ALAEGELEYN 

       250        260        270        280        290        300 
ENHKSIAAYV KFPLEKKSQM DLCKKLGITN NLPIYCLIWT STPWTLLSNR AICFNQDFSY 

       310        320        330        340        350        360 
SLLRLNSELI LVETGSIDKL GLTTNSFETI KQFQGTHLNG LYYQNLLVDD KVGRPLLHGA 

       370        380        390        400        410        420 
HVTSGTGTGL VHTAPGHGQD DYLIGIQNGL EIYSPVDHQG RYQLNELPQS VRSIVRDEGD 

       430        440        450        460        470        480 
LTKGRQVLDA ETAKIILCKL SDLNLLYKSH EYTHSYPYDW RSKKPVIIRA TPQWFADLHD 

       490        500        510        520        530        540 
VKNLALESIS RVKFCPKRGY SRLSSFMKSR NEWCISRQRS WGIPILSFYK KSEPDSVLMN 

       550        560        570        580        590        600 
SEILAHAIEK IKQKGINAWF NDKDNDMKEW LPEKYHDVAH EYCRSQDTMD VWFDSGSSWS 

       610        620        630        640        650        660 
VIKDFYEKSL KLSKLPSPLY QVCLEGSDQH RGWFQSSLLT KVASSNVPVA PYEEVITHGF 

       670        680        690        700        710        720 
TLDENGLKMS KSVGNTISPE AIIRGDENLG LPALGVDGLR YLIAQSNFTT DIVAGPTVMK 

       730        740        750        760        770        780 
HVGEALKKVR LTFRYLLSNL QKSQDFNLLP IEQLRRVDQY TLYKINELLE TTREHYQKYN 

       790        800        810        820        830        840 
FSKVLITLQY HLNNELSAFY FDISKDILYS NQISSLARRQ VQTTLVHILN AYRAILAPIL 

       850        860        870        880        890        900 
PVMVQEVWKY IPEGWLQGQE HIDINPMRGK WPFLDSNTEI VTSFENFELK ILKQFQEEFK 

       910        920        930        940        950        960 
RLSLEEGVTK TTHSHVTIFT KHHLPFSSDE LCDILQSSAV DILQMDDNNN SLPTIELGSG 

       970        980        990       1000 
INVQILVERS KRHNCPRCWK ANSAEEDKLC DRCKEAVDHL MS 

« Hide

References

« Hide 'large scale' references
[1]Tzagoloff A.A., Shtanko A.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24657 / D273-10B.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L38957 Genomic DNA. Translation: AAA64890.1.
U44030 Genomic DNA. Translation: AAB68180.1.
BK006949 Genomic DNA. Translation: DAA11389.1.
PIRS62035.
RefSeqNP_015285.1. NM_001183854.1.

3D structure databases

ProteinModelPortalP48526.
SMRP48526. Positions 49-458, 512-696.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36139. 16 interactions.
DIPDIP-4034N.
IntActP48526. 2 interactions.
MINTMINT-514793.
STRING4932.YPL040C.

Proteomic databases

PaxDbP48526.
PeptideAtlasP48526.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL040C; YPL040C; YPL040C.
GeneID856067.
KEGGsce:YPL040C.

Organism-specific databases

CYGDYPL040c.
SGDS000005961. ISM1.

Phylogenomic databases

eggNOGCOG0060.
GeneTreeENSGT00550000074910.
HOGENOMHOG000246402.
KOK01870.
OMAKGRSEWC.
OrthoDBEOG7JMGNT.

Enzyme and pathway databases

BioCycYEAST:G3O-33954-MONOMER.

Gene expression databases

GenevestigatorP48526.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981053.
PROP48526.

Entry information

Entry nameSYIM_YEAST
AccessionPrimary (citable) accession number: P48526
Secondary accession number(s): D6W3X3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries