P48526 (SYIM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase, mitochondrial
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ISM1
Ordered Locus Names:	YPL040C
ORF Names:	P7102.10

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1002 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 1270 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial isoleucyl-tRNA aminoacylation Inferred by curator PubMed 7607232. Source: SGD regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from sequence alignment PubMed 7607232. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1002	1002	Isoleucine--tRNA ligase, mitochondrial		PRO_0000098605
Regions
	Motif	94 – 104	11	"HIGH" region
	Motif	668 – 672	5	"KMSKS" region
Sites
	Binding site	671	1	ATP By similarity
Experimental info
	Sequence conflict	437	1	L → P in AAA64890. Ref.1
	Sequence conflict	672	1	S → L in AAA64890. Ref.1
	Sequence conflict	806	1	D → G in AAA64890. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P48526 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 2785B9DED636529E	FASTA	1,002	115,794
	10 20 30 40 50 60 MKRSRLVPQH IFSIISKRYL AKHAYQKTLN LPKTKFPNRS NLEITLRELI PKSSQLVYKE 70 80 90 100 110 120 QLRDFFEEFS KLNTTDEKLE FIKEKLFILH DGPPYANGEL HLGHALNKIL KDIINRYQLS 130 140 150 160 170 180 QGKYIFYKPG WDCHGLPIEI KALKDLSAQQ IESISPLKIR SMALKHAQKA IKRQRETFQH 190 200 210 220 230 240 FAILTDWETP YLTMDKDYEI NQLNIFKEMY ERGLIKRQNK PVYWGTETRT ALAEGELEYN 250 260 270 280 290 300 ENHKSIAAYV KFPLEKKSQM DLCKKLGITN NLPIYCLIWT STPWTLLSNR AICFNQDFSY 310 320 330 340 350 360 SLLRLNSELI LVETGSIDKL GLTTNSFETI KQFQGTHLNG LYYQNLLVDD KVGRPLLHGA 370 380 390 400 410 420 HVTSGTGTGL VHTAPGHGQD DYLIGIQNGL EIYSPVDHQG RYQLNELPQS VRSIVRDEGD 430 440 450 460 470 480 LTKGRQVLDA ETAKIILCKL SDLNLLYKSH EYTHSYPYDW RSKKPVIIRA TPQWFADLHD 490 500 510 520 530 540 VKNLALESIS RVKFCPKRGY SRLSSFMKSR NEWCISRQRS WGIPILSFYK KSEPDSVLMN 550 560 570 580 590 600 SEILAHAIEK IKQKGINAWF NDKDNDMKEW LPEKYHDVAH EYCRSQDTMD VWFDSGSSWS 610 620 630 640 650 660 VIKDFYEKSL KLSKLPSPLY QVCLEGSDQH RGWFQSSLLT KVASSNVPVA PYEEVITHGF 670 680 690 700 710 720 TLDENGLKMS KSVGNTISPE AIIRGDENLG LPALGVDGLR YLIAQSNFTT DIVAGPTVMK 730 740 750 760 770 780 HVGEALKKVR LTFRYLLSNL QKSQDFNLLP IEQLRRVDQY TLYKINELLE TTREHYQKYN 790 800 810 820 830 840 FSKVLITLQY HLNNELSAFY FDISKDILYS NQISSLARRQ VQTTLVHILN AYRAILAPIL 850 860 870 880 890 900 PVMVQEVWKY IPEGWLQGQE HIDINPMRGK WPFLDSNTEI VTSFENFELK ILKQFQEEFK 910 920 930 940 950 960 RLSLEEGVTK TTHSHVTIFT KHHLPFSSDE LCDILQSSAV DILQMDDNNN SLPTIELGSG 970 980 990 1000 INVQILVERS KRHNCPRCWK ANSAEEDKLC DRCKEAVDHL MS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Tzagoloff A.A., Shtanko A. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L38957 Genomic DNA. Translation: AAA64890.1. U44030 Genomic DNA. Translation: AAB68180.1. BK006949 Genomic DNA. Translation: DAA11389.1.
PIR	S62035.
RefSeq	NP_015285.1. NM_001183854.1.
3D structure databases
ProteinModelPortal	P48526.
SMR	P48526. Positions 74-458.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4034N.
IntAct	P48526. 3 interactions.
MINT	MINT-514793.
STRING	4932.YPL040C.
Proteomic databases
PaxDb	P48526.
PeptideAtlas	P48526.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL040C; YPL040C; YPL040C.
GeneID	856067.
KEGG	sce:YPL040C.
Organism-specific databases
CYGD	YPL040c.
SGD	S000005961. ISM1.
Phylogenomic databases
eggNOG	COG0060.
GeneTree	ENSGT00550000074910.
HOGENOM	HOG000246402.
KO	K01870.
OMA	DGVFTDV.
OrthoDB	EOG40S3PR.
Gene expression databases
ArrayExpress	P48526.
Genevestigator	P48526.
GermOnline	YPL040C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 2 hits.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981053.

Entry information

Entry name

SYIM_YEAST

Accession

Primary (citable) accession number: P48526
Secondary accession number(s): D6W3X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program