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Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

ISM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei671ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • isoleucyl-tRNA aminoacylation Source: SGD
  • mitochondrial isoleucyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33954-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ISM1
Ordered Locus Names:YPL040C
ORF Names:P7102.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL040C
SGDiS000005961 ISM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000986051 – 1002Isoleucine--tRNA ligase, mitochondrialAdd BLAST1002

Proteomic databases

MaxQBiP48526
PaxDbiP48526
PRIDEiP48526

Interactioni

Protein-protein interaction databases

BioGridi36139, 90 interactors
DIPiDIP-4034N
IntActiP48526, 3 interactors
MINTiP48526
STRINGi4932.YPL040C

Structurei

3D structure databases

ProteinModelPortaliP48526
SMRiP48526
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi94 – 104"HIGH" regionAdd BLAST11
Motifi668 – 672"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074910
HOGENOMiHOG000246402
InParanoidiP48526
KOiK01870
OMAiHLGTAWN
OrthoDBiEOG092C0SBX

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 3 hits
3.90.740.10, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P48526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSRLVPQH IFSIISKRYL AKHAYQKTLN LPKTKFPNRS NLEITLRELI
60 70 80 90 100
PKSSQLVYKE QLRDFFEEFS KLNTTDEKLE FIKEKLFILH DGPPYANGEL
110 120 130 140 150
HLGHALNKIL KDIINRYQLS QGKYIFYKPG WDCHGLPIEI KALKDLSAQQ
160 170 180 190 200
IESISPLKIR SMALKHAQKA IKRQRETFQH FAILTDWETP YLTMDKDYEI
210 220 230 240 250
NQLNIFKEMY ERGLIKRQNK PVYWGTETRT ALAEGELEYN ENHKSIAAYV
260 270 280 290 300
KFPLEKKSQM DLCKKLGITN NLPIYCLIWT STPWTLLSNR AICFNQDFSY
310 320 330 340 350
SLLRLNSELI LVETGSIDKL GLTTNSFETI KQFQGTHLNG LYYQNLLVDD
360 370 380 390 400
KVGRPLLHGA HVTSGTGTGL VHTAPGHGQD DYLIGIQNGL EIYSPVDHQG
410 420 430 440 450
RYQLNELPQS VRSIVRDEGD LTKGRQVLDA ETAKIILCKL SDLNLLYKSH
460 470 480 490 500
EYTHSYPYDW RSKKPVIIRA TPQWFADLHD VKNLALESIS RVKFCPKRGY
510 520 530 540 550
SRLSSFMKSR NEWCISRQRS WGIPILSFYK KSEPDSVLMN SEILAHAIEK
560 570 580 590 600
IKQKGINAWF NDKDNDMKEW LPEKYHDVAH EYCRSQDTMD VWFDSGSSWS
610 620 630 640 650
VIKDFYEKSL KLSKLPSPLY QVCLEGSDQH RGWFQSSLLT KVASSNVPVA
660 670 680 690 700
PYEEVITHGF TLDENGLKMS KSVGNTISPE AIIRGDENLG LPALGVDGLR
710 720 730 740 750
YLIAQSNFTT DIVAGPTVMK HVGEALKKVR LTFRYLLSNL QKSQDFNLLP
760 770 780 790 800
IEQLRRVDQY TLYKINELLE TTREHYQKYN FSKVLITLQY HLNNELSAFY
810 820 830 840 850
FDISKDILYS NQISSLARRQ VQTTLVHILN AYRAILAPIL PVMVQEVWKY
860 870 880 890 900
IPEGWLQGQE HIDINPMRGK WPFLDSNTEI VTSFENFELK ILKQFQEEFK
910 920 930 940 950
RLSLEEGVTK TTHSHVTIFT KHHLPFSSDE LCDILQSSAV DILQMDDNNN
960 970 980 990 1000
SLPTIELGSG INVQILVERS KRHNCPRCWK ANSAEEDKLC DRCKEAVDHL

MS
Length:1,002
Mass (Da):115,794
Last modified:October 1, 1996 - v2
Checksum:i2785B9DED636529E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti437L → P in AAA64890 (Ref. 1) Curated1
Sequence conflicti672S → L in AAA64890 (Ref. 1) Curated1
Sequence conflicti806D → G in AAA64890 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38957 Genomic DNA Translation: AAA64890.1
U44030 Genomic DNA Translation: AAB68180.1
BK006949 Genomic DNA Translation: DAA11389.1
PIRiS62035
RefSeqiNP_015285.1, NM_001183854.1

Genome annotation databases

EnsemblFungiiYPL040C; YPL040C; YPL040C
GeneIDi856067
KEGGisce:YPL040C

Similar proteinsi

Entry informationi

Entry nameiSYIM_YEAST
AccessioniPrimary (citable) accession number: P48526
Secondary accession number(s): D6W3X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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