Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase, mitochondrial

Gene

MSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58ATPBy similarity1
Binding sitei84GlutamateBy similarity1
Binding sitei253GlutamateBy similarity1
Binding sitei256ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi291 – 295ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • glutamyl-tRNA aminoacylation Source: SGD
  • mitochondrial glutamyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD
  • tRNA aminoacylation for mitochondrial protein translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33449-MONOMER.
SABIO-RKP48525.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:MSE1
Ordered Locus Names:YOL033W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL033W.
SGDiS000005393. MSE1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197401 – 536Glutamate--tRNA ligase, mitochondrialAdd BLAST536

Proteomic databases

MaxQBiP48525.
PRIDEiP48525.
TopDownProteomicsiP48525.

PTM databases

SwissPalmiP48525.

Interactioni

Protein-protein interaction databases

BioGridi34367. 10 interactors.
DIPiDIP-6368N.
IntActiP48525. 2 interactors.
MINTiMINT-607917.

Structurei

3D structure databases

ProteinModelPortaliP48525.
SMRiP48525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 50Glutamate bindingBy similarity3
Regioni235 – 239Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi53 – 61"HIGH" region9
Motifi291 – 295"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000009759.
HOGENOMiHOG000252720.
InParanoidiP48525.
KOiK01885.
OMAiKHIALYN.
OrthoDBiEOG092C0Y0C.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P48525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIMLRIPTRS YCSPSKLIKG VGLSPLKKSL LSKKIKEDIH PSLPVRTRFA
60 70 80 90 100
PSPTGFLHLG SLRTALYNYL LARNTNGQFL LRLEDTDQKR LIEGAEENIY
110 120 130 140 150
EILKWCNINY DETPIKQSER KLIYDKYVKI LLSSGKAYRC FCSKERLNDL
160 170 180 190 200
RHSAMELKPP SMASYDRCCA HLGEEEIKSK LAQGIPFTVR FKSPERYPTF
210 220 230 240 250
TDLLHGQINL QPQVNFNDKR YDDLILVKSD KLPTYHLANV VDDHLMGITH
260 270 280 290 300
VIRGEEWLPS TPKHIALYNA FGWACPKFIH IPLLTTVGDK KLSKRKGDMS
310 320 330 340 350
ISDLKRQGVL PEALINFCAL FGWSPPRDLA SKKHECFSME ELETIFNLNG
360 370 380 390 400
LTKGNAKVDD KKLWFFNKHF LQKRILNPST LRELVDDIMP SLESIYNTST
410 420 430 440 450
ISREKVAKIL LNCGGSLSRI NDFHDEFYYF FEKPKYNDND AVTKFLSKNE
460 470 480 490 500
SRHIAHLLKK LGQFQEGTDA QEVESMVETM YYENGFSRKV TYQAMRFALA
510 520 530
GCHPGAKIAA MIDILGIKES NKRLSEGLQF LQREKK
Length:536
Mass (Da):61,603
Last modified:November 1, 1997 - v2
Checksum:i5CF36FBAD0E8C58C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti464F → L in AAA61403 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39015 Genomic DNA. Translation: AAA61403.1.
Z74775 Genomic DNA. Translation: CAA99033.1.
BK006948 Genomic DNA. Translation: DAA10748.1.
PIRiS66716.
RefSeqiNP_014609.1. NM_001183287.1.

Genome annotation databases

EnsemblFungiiYOL033W; YOL033W; YOL033W.
GeneIDi854124.
KEGGisce:YOL033W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39015 Genomic DNA. Translation: AAA61403.1.
Z74775 Genomic DNA. Translation: CAA99033.1.
BK006948 Genomic DNA. Translation: DAA10748.1.
PIRiS66716.
RefSeqiNP_014609.1. NM_001183287.1.

3D structure databases

ProteinModelPortaliP48525.
SMRiP48525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34367. 10 interactors.
DIPiDIP-6368N.
IntActiP48525. 2 interactors.
MINTiMINT-607917.

PTM databases

SwissPalmiP48525.

Proteomic databases

MaxQBiP48525.
PRIDEiP48525.
TopDownProteomicsiP48525.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL033W; YOL033W; YOL033W.
GeneIDi854124.
KEGGisce:YOL033W.

Organism-specific databases

EuPathDBiFungiDB:YOL033W.
SGDiS000005393. MSE1.

Phylogenomic databases

GeneTreeiENSGT00390000009759.
HOGENOMiHOG000252720.
InParanoidiP48525.
KOiK01885.
OMAiKHIALYN.
OrthoDBiEOG092C0Y0C.

Enzyme and pathway databases

BioCyciYEAST:G3O-33449-MONOMER.
SABIO-RKP48525.

Miscellaneous databases

PROiP48525.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYEM_YEAST
AccessioniPrimary (citable) accession number: P48525
Secondary accession number(s): D6W232, Q08203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2940 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.