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P48525

- SYEM_YEAST

UniProt

P48525 - SYEM_YEAST

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Protein

Glutamate--tRNA ligase, mitochondrial

Gene

MSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581ATPBy similarity
Binding sitei84 – 841GlutamateBy similarity
Binding sitei253 – 2531GlutamateBy similarity
Binding sitei256 – 2561ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi291 – 2955ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-tRNA ligase activity Source: SGD
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: SGD
  2. mitochondrial glutamyl-tRNA aminoacylation Source: SGD
  3. mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33449-MONOMER.
SABIO-RKP48525.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:MSE1
Ordered Locus Names:YOL033W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL033w.
SGDiS000005393. MSE1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 536536Glutamate--tRNA ligase, mitochondrialPRO_0000119740Add
BLAST

Proteomic databases

MaxQBiP48525.
PaxDbiP48525.

Expressioni

Gene expression databases

GenevestigatoriP48525.

Interactioni

Protein-protein interaction databases

BioGridi34367. 9 interactions.
DIPiDIP-6368N.
IntActiP48525. 2 interactions.
MINTiMINT-607917.
STRINGi4932.YOL033W.

Structurei

3D structure databases

ProteinModelPortaliP48525.
SMRiP48525. Positions 45-533.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 503Glutamate bindingBy similarity
Regioni235 – 2395Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 619"HIGH" region
Motifi291 – 2955"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
GeneTreeiENSGT00390000009759.
HOGENOMiHOG000252720.
InParanoidiP48525.
KOiK01885.
OMAiKKLWFFN.
OrthoDBiEOG76MKJ7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P48525-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIMLRIPTRS YCSPSKLIKG VGLSPLKKSL LSKKIKEDIH PSLPVRTRFA
60 70 80 90 100
PSPTGFLHLG SLRTALYNYL LARNTNGQFL LRLEDTDQKR LIEGAEENIY
110 120 130 140 150
EILKWCNINY DETPIKQSER KLIYDKYVKI LLSSGKAYRC FCSKERLNDL
160 170 180 190 200
RHSAMELKPP SMASYDRCCA HLGEEEIKSK LAQGIPFTVR FKSPERYPTF
210 220 230 240 250
TDLLHGQINL QPQVNFNDKR YDDLILVKSD KLPTYHLANV VDDHLMGITH
260 270 280 290 300
VIRGEEWLPS TPKHIALYNA FGWACPKFIH IPLLTTVGDK KLSKRKGDMS
310 320 330 340 350
ISDLKRQGVL PEALINFCAL FGWSPPRDLA SKKHECFSME ELETIFNLNG
360 370 380 390 400
LTKGNAKVDD KKLWFFNKHF LQKRILNPST LRELVDDIMP SLESIYNTST
410 420 430 440 450
ISREKVAKIL LNCGGSLSRI NDFHDEFYYF FEKPKYNDND AVTKFLSKNE
460 470 480 490 500
SRHIAHLLKK LGQFQEGTDA QEVESMVETM YYENGFSRKV TYQAMRFALA
510 520 530
GCHPGAKIAA MIDILGIKES NKRLSEGLQF LQREKK
Length:536
Mass (Da):61,603
Last modified:November 1, 1997 - v2
Checksum:i5CF36FBAD0E8C58C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti464 – 4641F → L in AAA61403. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39015 Genomic DNA. Translation: AAA61403.1.
Z74775 Genomic DNA. Translation: CAA99033.1.
BK006948 Genomic DNA. Translation: DAA10748.1.
PIRiS66716.
RefSeqiNP_014609.1. NM_001183287.1.

Genome annotation databases

EnsemblFungiiYOL033W; YOL033W; YOL033W.
GeneIDi854124.
KEGGisce:YOL033W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39015 Genomic DNA. Translation: AAA61403.1 .
Z74775 Genomic DNA. Translation: CAA99033.1 .
BK006948 Genomic DNA. Translation: DAA10748.1 .
PIRi S66716.
RefSeqi NP_014609.1. NM_001183287.1.

3D structure databases

ProteinModelPortali P48525.
SMRi P48525. Positions 45-533.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34367. 9 interactions.
DIPi DIP-6368N.
IntActi P48525. 2 interactions.
MINTi MINT-607917.
STRINGi 4932.YOL033W.

Proteomic databases

MaxQBi P48525.
PaxDbi P48525.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL033W ; YOL033W ; YOL033W .
GeneIDi 854124.
KEGGi sce:YOL033W.

Organism-specific databases

CYGDi YOL033w.
SGDi S000005393. MSE1.

Phylogenomic databases

eggNOGi COG0008.
GeneTreei ENSGT00390000009759.
HOGENOMi HOG000252720.
InParanoidi P48525.
KOi K01885.
OMAi KKLWFFN.
OrthoDBi EOG76MKJ7.

Enzyme and pathway databases

BioCyci YEAST:G3O-33449-MONOMER.
SABIO-RK P48525.

Miscellaneous databases

NextBioi 975831.
PROi P48525.

Gene expression databases

Genevestigatori P48525.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Tzagoloff A.A., Shtanko A.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24657 / D273-10B.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYEM_YEAST
AccessioniPrimary (citable) accession number: P48525
Secondary accession number(s): D6W232, Q08203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2940 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3