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Protein

Glutamate--tRNA ligase, mitochondrial

Gene

MSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Miscellaneous

Present with 2940 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58ATPBy similarity1
Binding sitei84GlutamateBy similarity1
Binding sitei253GlutamateBy similarity1
Binding sitei256ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi291 – 295ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • glutamyl-tRNA aminoacylation Source: SGD
  • mitochondrial glutamyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33449-MONOMER
SABIO-RKP48525

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:MSE1
Ordered Locus Names:YOL033W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL033W
SGDiS000005393 MSE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197401 – 536Glutamate--tRNA ligase, mitochondrialAdd BLAST536

Proteomic databases

MaxQBiP48525
PaxDbiP48525
PRIDEiP48525
TopDownProteomicsiP48525

PTM databases

CarbonylDBiP48525
SwissPalmiP48525

Interactioni

Protein-protein interaction databases

BioGridi34367, 17 interactors
DIPiDIP-6368N
IntActiP48525, 3 interactors
MINTiP48525
STRINGi4932.YOL033W

Structurei

3D structure databases

ProteinModelPortaliP48525
SMRiP48525
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 50Glutamate bindingBy similarity3
Regioni235 – 239Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi53 – 61"HIGH" region9
Motifi291 – 295"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000009759
HOGENOMiHOG000252720
InParanoidiP48525
KOiK01885
OMAiAYYAFDT
OrthoDBiEOG092C0Y0C

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit

Sequencei

Sequence statusi: Complete.

P48525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIMLRIPTRS YCSPSKLIKG VGLSPLKKSL LSKKIKEDIH PSLPVRTRFA
60 70 80 90 100
PSPTGFLHLG SLRTALYNYL LARNTNGQFL LRLEDTDQKR LIEGAEENIY
110 120 130 140 150
EILKWCNINY DETPIKQSER KLIYDKYVKI LLSSGKAYRC FCSKERLNDL
160 170 180 190 200
RHSAMELKPP SMASYDRCCA HLGEEEIKSK LAQGIPFTVR FKSPERYPTF
210 220 230 240 250
TDLLHGQINL QPQVNFNDKR YDDLILVKSD KLPTYHLANV VDDHLMGITH
260 270 280 290 300
VIRGEEWLPS TPKHIALYNA FGWACPKFIH IPLLTTVGDK KLSKRKGDMS
310 320 330 340 350
ISDLKRQGVL PEALINFCAL FGWSPPRDLA SKKHECFSME ELETIFNLNG
360 370 380 390 400
LTKGNAKVDD KKLWFFNKHF LQKRILNPST LRELVDDIMP SLESIYNTST
410 420 430 440 450
ISREKVAKIL LNCGGSLSRI NDFHDEFYYF FEKPKYNDND AVTKFLSKNE
460 470 480 490 500
SRHIAHLLKK LGQFQEGTDA QEVESMVETM YYENGFSRKV TYQAMRFALA
510 520 530
GCHPGAKIAA MIDILGIKES NKRLSEGLQF LQREKK
Length:536
Mass (Da):61,603
Last modified:November 1, 1997 - v2
Checksum:i5CF36FBAD0E8C58C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti464F → L in AAA61403 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39015 Genomic DNA Translation: AAA61403.1
Z74775 Genomic DNA Translation: CAA99033.1
BK006948 Genomic DNA Translation: DAA10748.1
PIRiS66716
RefSeqiNP_014609.1, NM_001183287.1

Genome annotation databases

EnsemblFungiiYOL033W; YOL033W; YOL033W
GeneIDi854124
KEGGisce:YOL033W

Similar proteinsi

Entry informationi

Entry nameiSYEM_YEAST
AccessioniPrimary (citable) accession number: P48525
Secondary accession number(s): D6W232, Q08203
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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