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P48525

- SYEM_YEAST

UniProt

P48525 - SYEM_YEAST

Protein

Glutamate--tRNA ligase, mitochondrial

Gene

MSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei58 – 581ATPBy similarity
    Binding sitei84 – 841GlutamateBy similarity
    Binding sitei253 – 2531GlutamateBy similarity
    Binding sitei256 – 2561ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi291 – 2955ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-tRNA ligase activity Source: SGD
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: SGD
    2. mitochondrial glutamyl-tRNA aminoacylation Source: SGD
    3. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33449-MONOMER.
    SABIO-RKP48525.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS
    Gene namesi
    Name:MSE1
    Ordered Locus Names:YOL033W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL033w.
    SGDiS000005393. MSE1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 536536Glutamate--tRNA ligase, mitochondrialPRO_0000119740Add
    BLAST

    Proteomic databases

    MaxQBiP48525.
    PaxDbiP48525.

    Expressioni

    Gene expression databases

    GenevestigatoriP48525.

    Interactioni

    Protein-protein interaction databases

    BioGridi34367. 9 interactions.
    DIPiDIP-6368N.
    IntActiP48525. 2 interactions.
    MINTiMINT-607917.
    STRINGi4932.YOL033W.

    Structurei

    3D structure databases

    ProteinModelPortaliP48525.
    SMRiP48525. Positions 45-533.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 503Glutamate bindingBy similarity
    Regioni235 – 2395Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi53 – 619"HIGH" region
    Motifi291 – 2955"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0008.
    GeneTreeiENSGT00390000009759.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiKKLWFFN.
    OrthoDBiEOG76MKJ7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P48525-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIMLRIPTRS YCSPSKLIKG VGLSPLKKSL LSKKIKEDIH PSLPVRTRFA    50
    PSPTGFLHLG SLRTALYNYL LARNTNGQFL LRLEDTDQKR LIEGAEENIY 100
    EILKWCNINY DETPIKQSER KLIYDKYVKI LLSSGKAYRC FCSKERLNDL 150
    RHSAMELKPP SMASYDRCCA HLGEEEIKSK LAQGIPFTVR FKSPERYPTF 200
    TDLLHGQINL QPQVNFNDKR YDDLILVKSD KLPTYHLANV VDDHLMGITH 250
    VIRGEEWLPS TPKHIALYNA FGWACPKFIH IPLLTTVGDK KLSKRKGDMS 300
    ISDLKRQGVL PEALINFCAL FGWSPPRDLA SKKHECFSME ELETIFNLNG 350
    LTKGNAKVDD KKLWFFNKHF LQKRILNPST LRELVDDIMP SLESIYNTST 400
    ISREKVAKIL LNCGGSLSRI NDFHDEFYYF FEKPKYNDND AVTKFLSKNE 450
    SRHIAHLLKK LGQFQEGTDA QEVESMVETM YYENGFSRKV TYQAMRFALA 500
    GCHPGAKIAA MIDILGIKES NKRLSEGLQF LQREKK 536
    Length:536
    Mass (Da):61,603
    Last modified:November 1, 1997 - v2
    Checksum:i5CF36FBAD0E8C58C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti464 – 4641F → L in AAA61403. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L39015 Genomic DNA. Translation: AAA61403.1.
    Z74775 Genomic DNA. Translation: CAA99033.1.
    BK006948 Genomic DNA. Translation: DAA10748.1.
    PIRiS66716.
    RefSeqiNP_014609.1. NM_001183287.1.

    Genome annotation databases

    EnsemblFungiiYOL033W; YOL033W; YOL033W.
    GeneIDi854124.
    KEGGisce:YOL033W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L39015 Genomic DNA. Translation: AAA61403.1 .
    Z74775 Genomic DNA. Translation: CAA99033.1 .
    BK006948 Genomic DNA. Translation: DAA10748.1 .
    PIRi S66716.
    RefSeqi NP_014609.1. NM_001183287.1.

    3D structure databases

    ProteinModelPortali P48525.
    SMRi P48525. Positions 45-533.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34367. 9 interactions.
    DIPi DIP-6368N.
    IntActi P48525. 2 interactions.
    MINTi MINT-607917.
    STRINGi 4932.YOL033W.

    Proteomic databases

    MaxQBi P48525.
    PaxDbi P48525.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL033W ; YOL033W ; YOL033W .
    GeneIDi 854124.
    KEGGi sce:YOL033W.

    Organism-specific databases

    CYGDi YOL033w.
    SGDi S000005393. MSE1.

    Phylogenomic databases

    eggNOGi COG0008.
    GeneTreei ENSGT00390000009759.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi KKLWFFN.
    OrthoDBi EOG76MKJ7.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33449-MONOMER.
    SABIO-RK P48525.

    Miscellaneous databases

    NextBioi 975831.
    PROi P48525.

    Gene expression databases

    Genevestigatori P48525.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Tzagoloff A.A., Shtanko A.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 24657 / D273-10B.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYEM_YEAST
    AccessioniPrimary (citable) accession number: P48525
    Secondary accession number(s): D6W232, Q08203
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2940 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3