Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P48524

- BUL1_YEAST

UniProt

P48524 - BUL1_YEAST

Protein

Ubiquitin ligase-binding protein BUL1

Gene

BUL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other permeases such as PMA1. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription and pressure-induced differential regulation of the 2 tryptophan permeases TAT1 and TAT2.11 Publications

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin-ubiquitin ligase activity Source: SGD

    GO - Biological processi

    1. mitochondrion inheritance Source: SGD
    2. protein monoubiquitination Source: SGD
    3. protein polyubiquitination Source: SGD

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32946-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin ligase-binding protein BUL1
    Alternative name(s):
    Respiration deficiency suppressor 1
    Gene namesi
    Name:BUL1
    Synonyms:DAG1, RDS1, SMM2
    Ordered Locus Names:YMR275C
    ORF Names:YM8021.01C, YM8156.17C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR275c.
    SGDiS000004888. BUL1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: SGD
    3. ubiquitin ligase complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi157 – 1571P → Q: Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with A-158. 2 Publications
    Mutagenesisi158 – 1581P → A: Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with Q-157. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 976976Ubiquitin ligase-binding protein BUL1PRO_0000065022Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 581Phosphoserine1 Publication
    Modified residuei70 – 701Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP48524.
    PaxDbiP48524.
    PeptideAtlasiP48524.

    Expressioni

    Gene expression databases

    GenevestigatoriP48524.

    Interactioni

    Subunit structurei

    Component of the RSP5-BUL1/2 ubiquitin ligase complex composed of at least RSP5 and BUL1 or BUL2.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RPS5P267833EBI-3881,EBI-16150

    Protein-protein interaction databases

    BioGridi35454. 118 interactions.
    DIPiDIP-2502N.
    IntActiP48524. 11 interactions.
    MINTiMINT-596689.
    STRINGi4932.YMR275C.

    Structurei

    3D structure databases

    ProteinModelPortaliP48524.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi156 – 1605PY-motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi631 – 64010Poly-Ser
    Compositional biasi868 – 8769Poly-Ser

    Domaini

    The PY-motif is required for the interaction with RSP5 ubiquitin-ligase and the HSE-mediated gene expression.1 Publication

    Sequence similaritiesi

    Belongs to the BUL1 family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG43698.
    GeneTreeiENSGT00390000010225.
    HOGENOMiHOG000095282.
    OMAiFESCLCC.
    OrthoDBiEOG7PK975.

    Family and domain databases

    InterProiIPR022794. Bul1_C.
    IPR007520. Bul1_C_yeast.
    IPR007519. Bul1_N.
    [Graphical view]
    PfamiPF04426. Bul1_C. 1 hit.
    PF04425. Bul1_N. 1 hit.
    [Graphical view]
    ProDomiPD024065. Bul1_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    P48524-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKDLNDSGF PPKRKPLLRP QRSDFTANSS TTMNVNANTR GRGRQKQEGG    50
    KGSSRSPSLH SPKSWIRSAS ATGILGLRRP ELAHSHSHAP STGTPAGGNR 100
    SPLRRSTANA TPVETGRSLT DGDINNVVDV LPSFEMYNTL HRHIPQGNVD 150
    PDRHDFPPSY QEANNSTATG AAGSSADLSH QSLSTDALGA TRSSSTSNLE 200
    NLIPLRTEHH SIAAHQSTAV DEDSLDIPPI LDDLNDTDNI FIDKLYTLPK 250
    MSTPIEITIK TTKHAPIPHV KPEEESILKE YTSGDLIHGF ITIENKSQAN 300
    LKFEMFYVTL ESYISIIDKV KSKRTIKRFL RMVDLSASWS YSKIALGSGV 350
    DFIPADVDYD GSVFGLNNSR VLEPGVKYKK FFIFKLPLQL LDVTCKQEHF 400
    SHCLLPPSFG IDKYRNNCKY SGIKVNRVLG CGHLGTKGSP ILTNDMSDDN 450
    LSINYTIDAR IVGKDQKASK LYIMKEREYN LRVIPFGFDA NVVGERTTMS 500
    QLNDITKLVQ ERLDALRKIF QRLEKKEPIT NRDIHGADLS GTIDDSIESD 550
    SQEILQRKLD QLHIKNRNNY LVNYNDLKLG HDLDNGRSGN SGHNTDTSRA 600
    WGPFVESELK YKLKNKSNSS SFLNFSHFLN SSSSSMSSSS NAGKNNHDLT 650
    GNKERTGLIL VKAKIPKQGL PYWAPSLLRK TNVFESKSKH DQENWVRLSE 700
    LIPEDVKKPL EKLDLQLTCI ESDNSLPHDP PEIQSITTEL ICITAKSDNS 750
    IPIKLNSELL MNKEKLTSIK ALYDDFHSKI CEYETKFNKN FLELNELYNM 800
    NRGDRRPKEL KFTDFITSQL FNDIESICNL KVSVHNLSNI FKKQVSTLKQ 850
    HSKHALSEDS ISHTGNGSSS SPSSASLTPV TSSSKSSLFL PSGSSSTSLK 900
    FTDQIVHKWV RIAPLQYKRD INVNLEFNKD IKETLIPSFE SCLCCRFYCV 950
    RVMIKFENHL GVAKIDIPIS VRQVTK 976
    Length:976
    Mass (Da):109,174
    Last modified:February 1, 1996 - v1
    Checksum:i9253D207B894CE44
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351V → A in BAA08787. (PubMed:8668140)Curated
    Sequence conflicti773 – 7753YDD → ISN in AAB07266. 1 PublicationCurated
    Sequence conflicti963 – 9631A → R in CAA61363. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50083 Genomic DNA. Translation: BAA08787.1.
    X88901 Genomic DNA. Translation: CAA61363.1.
    Z49704 Genomic DNA. Translation: CAA89773.1.
    Z49260 Genomic DNA. Translation: CAA89258.1.
    L40587 Genomic DNA. Translation: AAB07266.1.
    BK006946 Genomic DNA. Translation: DAA10176.1.
    PIRiS57725.
    RefSeqiNP_014002.1. NM_001182782.1.

    Genome annotation databases

    EnsemblFungiiYMR275C; YMR275C; YMR275C.
    GeneIDi855318.
    KEGGisce:YMR275C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50083 Genomic DNA. Translation: BAA08787.1 .
    X88901 Genomic DNA. Translation: CAA61363.1 .
    Z49704 Genomic DNA. Translation: CAA89773.1 .
    Z49260 Genomic DNA. Translation: CAA89258.1 .
    L40587 Genomic DNA. Translation: AAB07266.1 .
    BK006946 Genomic DNA. Translation: DAA10176.1 .
    PIRi S57725.
    RefSeqi NP_014002.1. NM_001182782.1.

    3D structure databases

    ProteinModelPortali P48524.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35454. 118 interactions.
    DIPi DIP-2502N.
    IntActi P48524. 11 interactions.
    MINTi MINT-596689.
    STRINGi 4932.YMR275C.

    Proteomic databases

    MaxQBi P48524.
    PaxDbi P48524.
    PeptideAtlasi P48524.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR275C ; YMR275C ; YMR275C .
    GeneIDi 855318.
    KEGGi sce:YMR275C.

    Organism-specific databases

    CYGDi YMR275c.
    SGDi S000004888. BUL1.

    Phylogenomic databases

    eggNOGi NOG43698.
    GeneTreei ENSGT00390000010225.
    HOGENOMi HOG000095282.
    OMAi FESCLCC.
    OrthoDBi EOG7PK975.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-32946-MONOMER.

    Miscellaneous databases

    NextBioi 979017.

    Gene expression databases

    Genevestigatori P48524.

    Family and domain databases

    InterProi IPR022794. Bul1_C.
    IPR007520. Bul1_C_yeast.
    IPR007519. Bul1_N.
    [Graphical view ]
    Pfami PF04426. Bul1_C. 1 hit.
    PF04425. Bul1_N. 1 hit.
    [Graphical view ]
    ProDomi PD024065. Bul1_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae."
      Yashiroda H., Oguchi T., Yasuda Y., Toh-e A., Kikuchi Y.
      Mol. Cell. Biol. 16:3255-3263(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH RSP5.
    2. Stein T.
      Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Biggins S., Rose M.D.
      Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-776.
      Strain: ATCC 204508 / S288c.
    6. "The PY-motif of Bul1 protein is essential for growth of Saccharomyces cerevisiae under various stress conditions."
      Yashiroda H., Kaida D., Toh-e A., Kikuchi Y.
      Gene 225:39-46(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DOMAIN, INTERACTION WITH RSP5, MUTAGENESIS OF PRO-157 AND PRO-158.
    7. "Yeast glycogen synthase kinase 3 is involved in protein degradation in cooperation with Bul1, Bul2, and Rsp5."
      Andoh T., Hirata Y., Kikuchi A.
      Mol. Cell. Biol. 20:6712-6720(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Ubiquitin is required for sorting to the vacuole of the yeast general amino acid permease, Gap1."
      Soetens O., De Craene J.-O., Andre B.
      J. Biol. Chem. 276:43949-43957(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease."
      Helliwell S.B., Losko S., Kaiser C.A.
      J. Cell Biol. 153:649-662(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Rsp5-Bul1/2 complex is necessary for the HSE-mediated gene expression in budding yeast."
      Kaida D., Toh-e A., Kikuchi Y.
      Biochem. Biophys. Res. Commun. 306:1037-1041(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX, MUTAGENESIS OF PRO-157 AND PRO-158.
    11. "Pressure-induced differential regulation of the two tryptophan permeases Tat1 and Tat2 by ubiquitin ligase Rsp5 and its binding proteins, Bul1 and Bul2."
      Abe F., Iida H.
      Mol. Cell. Biol. 23:7566-7584(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX.
    12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    14. "Genetic, biochemical, and transcriptional responses of Saccharomyces cerevisiae to the novel immunomodulator FTY720 largely mimic those of the natural sphingolipid phytosphingosine."
      Welsch C.A., Roth L.W.A., Goetschy J.F., Movva N.R.
      J. Biol. Chem. 279:36720-36731(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "NPR1 kinase and RSP5-BUL1/2 ubiquitin ligase control GLN3-dependent transcription in Saccharomyces cerevisiae."
      Crespo J.L., Helliwell S.B., Wiederkehr C., Demougin P., Fowler B., Primig M., Hall M.N.
      J. Biol. Chem. 279:37512-37517(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX.
    16. "Ubiquitin-mediated targeting of a mutant plasma membrane ATPase, Pma1-7, to the endosomal/vacuolar system in yeast."
      Pizzirusso M., Chang A.
      Mol. Biol. Cell 15:2401-2409(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX.
    17. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    18. "Transduction of the nitrogen signal activating Gln3-mediated transcription is independent of Npr1 kinase and Rsp5-Bul1/2 ubiquitin ligase in Saccharomyces cerevisiae."
      Feller A., Boeckstaens M., Marini A.-M., Dubois E.
      J. Biol. Chem. 281:28546-28554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX.
    19. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    20. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    21. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBUL1_YEAST
    AccessioniPrimary (citable) accession number: P48524
    Secondary accession number(s): D6W0A2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 486 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3