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Protein

Ubiquitin ligase-binding protein BUL1

Gene

BUL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other permeases such as PMA1. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription and pressure-induced differential regulation of the 2 tryptophan permeases TAT1 and TAT2.11 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin-ubiquitin ligase activity Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • mitochondrion inheritance Source: SGD
  • protein monoubiquitination Source: SGD
  • protein polyubiquitination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32946-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin ligase-binding protein BUL1
Alternative name(s):
Respiration deficiency suppressor 1
Gene namesi
Name:BUL1
Synonyms:DAG1, RDS1, SMM2
Ordered Locus Names:YMR275C
ORF Names:YM8021.01C, YM8156.17C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR275C.
SGDiS000004888. BUL1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: SGD
  • ubiquitin ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157P → Q: Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with A-158. 2 Publications1
Mutagenesisi158P → A: Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with Q-157. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000650221 – 976Ubiquitin ligase-binding protein BUL1Add BLAST976

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei70PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48524.
PRIDEiP48524.

PTM databases

iPTMnetiP48524.

Interactioni

Subunit structurei

Component of the RSP5-BUL1/2 ubiquitin ligase complex composed of at least RSP5 and BUL1 or BUL2.

Binary interactionsi

WithEntry#Exp.IntActNotes
RPS5P267833EBI-3881,EBI-16150

Protein-protein interaction databases

BioGridi35454. 119 interactors.
DIPiDIP-2502N.
IntActiP48524. 11 interactors.
MINTiMINT-596689.

Structurei

3D structure databases

ProteinModelPortaliP48524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi156 – 160PY-motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi631 – 640Poly-Ser10
Compositional biasi868 – 876Poly-Ser9

Domaini

The PY-motif is required for the interaction with RSP5 ubiquitin-ligase and the HSE-mediated gene expression.1 Publication

Sequence similaritiesi

Belongs to the BUL1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000010225.
HOGENOMiHOG000095282.
InParanoidiP48524.
OMAiFESCLCC.
OrthoDBiEOG092C0V39.

Family and domain databases

InterProiIPR022794. Bul1_C.
IPR007519. Bul1_N.
[Graphical view]
PfamiPF04426. Bul1_C. 1 hit.
PF04425. Bul1_N. 1 hit.
[Graphical view]
ProDomiPD024065. Bul1_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P48524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKDLNDSGF PPKRKPLLRP QRSDFTANSS TTMNVNANTR GRGRQKQEGG
60 70 80 90 100
KGSSRSPSLH SPKSWIRSAS ATGILGLRRP ELAHSHSHAP STGTPAGGNR
110 120 130 140 150
SPLRRSTANA TPVETGRSLT DGDINNVVDV LPSFEMYNTL HRHIPQGNVD
160 170 180 190 200
PDRHDFPPSY QEANNSTATG AAGSSADLSH QSLSTDALGA TRSSSTSNLE
210 220 230 240 250
NLIPLRTEHH SIAAHQSTAV DEDSLDIPPI LDDLNDTDNI FIDKLYTLPK
260 270 280 290 300
MSTPIEITIK TTKHAPIPHV KPEEESILKE YTSGDLIHGF ITIENKSQAN
310 320 330 340 350
LKFEMFYVTL ESYISIIDKV KSKRTIKRFL RMVDLSASWS YSKIALGSGV
360 370 380 390 400
DFIPADVDYD GSVFGLNNSR VLEPGVKYKK FFIFKLPLQL LDVTCKQEHF
410 420 430 440 450
SHCLLPPSFG IDKYRNNCKY SGIKVNRVLG CGHLGTKGSP ILTNDMSDDN
460 470 480 490 500
LSINYTIDAR IVGKDQKASK LYIMKEREYN LRVIPFGFDA NVVGERTTMS
510 520 530 540 550
QLNDITKLVQ ERLDALRKIF QRLEKKEPIT NRDIHGADLS GTIDDSIESD
560 570 580 590 600
SQEILQRKLD QLHIKNRNNY LVNYNDLKLG HDLDNGRSGN SGHNTDTSRA
610 620 630 640 650
WGPFVESELK YKLKNKSNSS SFLNFSHFLN SSSSSMSSSS NAGKNNHDLT
660 670 680 690 700
GNKERTGLIL VKAKIPKQGL PYWAPSLLRK TNVFESKSKH DQENWVRLSE
710 720 730 740 750
LIPEDVKKPL EKLDLQLTCI ESDNSLPHDP PEIQSITTEL ICITAKSDNS
760 770 780 790 800
IPIKLNSELL MNKEKLTSIK ALYDDFHSKI CEYETKFNKN FLELNELYNM
810 820 830 840 850
NRGDRRPKEL KFTDFITSQL FNDIESICNL KVSVHNLSNI FKKQVSTLKQ
860 870 880 890 900
HSKHALSEDS ISHTGNGSSS SPSSASLTPV TSSSKSSLFL PSGSSSTSLK
910 920 930 940 950
FTDQIVHKWV RIAPLQYKRD INVNLEFNKD IKETLIPSFE SCLCCRFYCV
960 970
RVMIKFENHL GVAKIDIPIS VRQVTK
Length:976
Mass (Da):109,174
Last modified:February 1, 1996 - v1
Checksum:i9253D207B894CE44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35V → A in BAA08787 (PubMed:8668140).Curated1
Sequence conflicti773 – 775YDD → ISN in AAB07266 (Ref. 5) Curated3
Sequence conflicti963A → R in CAA61363 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50083 Genomic DNA. Translation: BAA08787.1.
X88901 Genomic DNA. Translation: CAA61363.1.
Z49704 Genomic DNA. Translation: CAA89773.1.
Z49260 Genomic DNA. Translation: CAA89258.1.
L40587 Genomic DNA. Translation: AAB07266.1.
BK006946 Genomic DNA. Translation: DAA10176.1.
PIRiS57725.
RefSeqiNP_014002.1. NM_001182782.1.

Genome annotation databases

EnsemblFungiiYMR275C; YMR275C; YMR275C.
GeneIDi855318.
KEGGisce:YMR275C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50083 Genomic DNA. Translation: BAA08787.1.
X88901 Genomic DNA. Translation: CAA61363.1.
Z49704 Genomic DNA. Translation: CAA89773.1.
Z49260 Genomic DNA. Translation: CAA89258.1.
L40587 Genomic DNA. Translation: AAB07266.1.
BK006946 Genomic DNA. Translation: DAA10176.1.
PIRiS57725.
RefSeqiNP_014002.1. NM_001182782.1.

3D structure databases

ProteinModelPortaliP48524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35454. 119 interactors.
DIPiDIP-2502N.
IntActiP48524. 11 interactors.
MINTiMINT-596689.

PTM databases

iPTMnetiP48524.

Proteomic databases

MaxQBiP48524.
PRIDEiP48524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR275C; YMR275C; YMR275C.
GeneIDi855318.
KEGGisce:YMR275C.

Organism-specific databases

EuPathDBiFungiDB:YMR275C.
SGDiS000004888. BUL1.

Phylogenomic databases

GeneTreeiENSGT00390000010225.
HOGENOMiHOG000095282.
InParanoidiP48524.
OMAiFESCLCC.
OrthoDBiEOG092C0V39.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-32946-MONOMER.

Miscellaneous databases

PROiP48524.

Family and domain databases

InterProiIPR022794. Bul1_C.
IPR007519. Bul1_N.
[Graphical view]
PfamiPF04426. Bul1_C. 1 hit.
PF04425. Bul1_N. 1 hit.
[Graphical view]
ProDomiPD024065. Bul1_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiBUL1_YEAST
AccessioniPrimary (citable) accession number: P48524
Secondary accession number(s): D6W0A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 486 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.