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P48524

- BUL1_YEAST

UniProt

P48524 - BUL1_YEAST

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Protein

Ubiquitin ligase-binding protein BUL1

Gene

BUL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other permeases such as PMA1. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription and pressure-induced differential regulation of the 2 tryptophan permeases TAT1 and TAT2.11 Publications

Pathwayi

GO - Molecular functioni

  1. ubiquitin-ubiquitin ligase activity Source: SGD

GO - Biological processi

  1. mitochondrion inheritance Source: SGD
  2. protein monoubiquitination Source: SGD
  3. protein polyubiquitination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32946-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin ligase-binding protein BUL1
Alternative name(s):
Respiration deficiency suppressor 1
Gene namesi
Name:BUL1
Synonyms:DAG1, RDS1, SMM2
Ordered Locus Names:YMR275C
ORF Names:YM8021.01C, YM8156.17C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR275c.
SGDiS000004888. BUL1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. plasma membrane Source: SGD
  3. ubiquitin ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi157 – 1571P → Q: Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with A-158. 2 Publications
Mutagenesisi158 – 1581P → A: Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with Q-157. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 976976Ubiquitin ligase-binding protein BUL1PRO_0000065022Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Phosphoserine1 Publication
Modified residuei70 – 701Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP48524.
PaxDbiP48524.
PeptideAtlasiP48524.

Expressioni

Gene expression databases

GenevestigatoriP48524.

Interactioni

Subunit structurei

Component of the RSP5-BUL1/2 ubiquitin ligase complex composed of at least RSP5 and BUL1 or BUL2.

Binary interactionsi

WithEntry#Exp.IntActNotes
RPS5P267833EBI-3881,EBI-16150

Protein-protein interaction databases

BioGridi35454. 118 interactions.
DIPiDIP-2502N.
IntActiP48524. 11 interactions.
MINTiMINT-596689.
STRINGi4932.YMR275C.

Structurei

3D structure databases

ProteinModelPortaliP48524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi156 – 1605PY-motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi631 – 64010Poly-Ser
Compositional biasi868 – 8769Poly-Ser

Domaini

The PY-motif is required for the interaction with RSP5 ubiquitin-ligase and the HSE-mediated gene expression.1 Publication

Sequence similaritiesi

Belongs to the BUL1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG43698.
GeneTreeiENSGT00390000010225.
HOGENOMiHOG000095282.
InParanoidiP48524.
OMAiFESCLCC.
OrthoDBiEOG7PK975.

Family and domain databases

InterProiIPR022794. Bul1_C.
IPR007520. Bul1_C_yeast.
IPR007519. Bul1_N.
[Graphical view]
PfamiPF04426. Bul1_C. 1 hit.
PF04425. Bul1_N. 1 hit.
[Graphical view]
ProDomiPD024065. Bul1_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P48524-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKDLNDSGF PPKRKPLLRP QRSDFTANSS TTMNVNANTR GRGRQKQEGG
60 70 80 90 100
KGSSRSPSLH SPKSWIRSAS ATGILGLRRP ELAHSHSHAP STGTPAGGNR
110 120 130 140 150
SPLRRSTANA TPVETGRSLT DGDINNVVDV LPSFEMYNTL HRHIPQGNVD
160 170 180 190 200
PDRHDFPPSY QEANNSTATG AAGSSADLSH QSLSTDALGA TRSSSTSNLE
210 220 230 240 250
NLIPLRTEHH SIAAHQSTAV DEDSLDIPPI LDDLNDTDNI FIDKLYTLPK
260 270 280 290 300
MSTPIEITIK TTKHAPIPHV KPEEESILKE YTSGDLIHGF ITIENKSQAN
310 320 330 340 350
LKFEMFYVTL ESYISIIDKV KSKRTIKRFL RMVDLSASWS YSKIALGSGV
360 370 380 390 400
DFIPADVDYD GSVFGLNNSR VLEPGVKYKK FFIFKLPLQL LDVTCKQEHF
410 420 430 440 450
SHCLLPPSFG IDKYRNNCKY SGIKVNRVLG CGHLGTKGSP ILTNDMSDDN
460 470 480 490 500
LSINYTIDAR IVGKDQKASK LYIMKEREYN LRVIPFGFDA NVVGERTTMS
510 520 530 540 550
QLNDITKLVQ ERLDALRKIF QRLEKKEPIT NRDIHGADLS GTIDDSIESD
560 570 580 590 600
SQEILQRKLD QLHIKNRNNY LVNYNDLKLG HDLDNGRSGN SGHNTDTSRA
610 620 630 640 650
WGPFVESELK YKLKNKSNSS SFLNFSHFLN SSSSSMSSSS NAGKNNHDLT
660 670 680 690 700
GNKERTGLIL VKAKIPKQGL PYWAPSLLRK TNVFESKSKH DQENWVRLSE
710 720 730 740 750
LIPEDVKKPL EKLDLQLTCI ESDNSLPHDP PEIQSITTEL ICITAKSDNS
760 770 780 790 800
IPIKLNSELL MNKEKLTSIK ALYDDFHSKI CEYETKFNKN FLELNELYNM
810 820 830 840 850
NRGDRRPKEL KFTDFITSQL FNDIESICNL KVSVHNLSNI FKKQVSTLKQ
860 870 880 890 900
HSKHALSEDS ISHTGNGSSS SPSSASLTPV TSSSKSSLFL PSGSSSTSLK
910 920 930 940 950
FTDQIVHKWV RIAPLQYKRD INVNLEFNKD IKETLIPSFE SCLCCRFYCV
960 970
RVMIKFENHL GVAKIDIPIS VRQVTK
Length:976
Mass (Da):109,174
Last modified:February 1, 1996 - v1
Checksum:i9253D207B894CE44
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351V → A in BAA08787. (PubMed:8668140)Curated
Sequence conflicti773 – 7753YDD → ISN in AAB07266. 1 PublicationCurated
Sequence conflicti963 – 9631A → R in CAA61363. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50083 Genomic DNA. Translation: BAA08787.1.
X88901 Genomic DNA. Translation: CAA61363.1.
Z49704 Genomic DNA. Translation: CAA89773.1.
Z49260 Genomic DNA. Translation: CAA89258.1.
L40587 Genomic DNA. Translation: AAB07266.1.
BK006946 Genomic DNA. Translation: DAA10176.1.
PIRiS57725.
RefSeqiNP_014002.1. NM_001182782.1.

Genome annotation databases

EnsemblFungiiYMR275C; YMR275C; YMR275C.
GeneIDi855318.
KEGGisce:YMR275C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50083 Genomic DNA. Translation: BAA08787.1 .
X88901 Genomic DNA. Translation: CAA61363.1 .
Z49704 Genomic DNA. Translation: CAA89773.1 .
Z49260 Genomic DNA. Translation: CAA89258.1 .
L40587 Genomic DNA. Translation: AAB07266.1 .
BK006946 Genomic DNA. Translation: DAA10176.1 .
PIRi S57725.
RefSeqi NP_014002.1. NM_001182782.1.

3D structure databases

ProteinModelPortali P48524.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35454. 118 interactions.
DIPi DIP-2502N.
IntActi P48524. 11 interactions.
MINTi MINT-596689.
STRINGi 4932.YMR275C.

Proteomic databases

MaxQBi P48524.
PaxDbi P48524.
PeptideAtlasi P48524.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR275C ; YMR275C ; YMR275C .
GeneIDi 855318.
KEGGi sce:YMR275C.

Organism-specific databases

CYGDi YMR275c.
SGDi S000004888. BUL1.

Phylogenomic databases

eggNOGi NOG43698.
GeneTreei ENSGT00390000010225.
HOGENOMi HOG000095282.
InParanoidi P48524.
OMAi FESCLCC.
OrthoDBi EOG7PK975.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci YEAST:G3O-32946-MONOMER.

Miscellaneous databases

NextBioi 979017.

Gene expression databases

Genevestigatori P48524.

Family and domain databases

InterProi IPR022794. Bul1_C.
IPR007520. Bul1_C_yeast.
IPR007519. Bul1_N.
[Graphical view ]
Pfami PF04426. Bul1_C. 1 hit.
PF04425. Bul1_N. 1 hit.
[Graphical view ]
ProDomi PD024065. Bul1_C. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae."
    Yashiroda H., Oguchi T., Yasuda Y., Toh-e A., Kikuchi Y.
    Mol. Cell. Biol. 16:3255-3263(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH RSP5.
  2. Stein T.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Biggins S., Rose M.D.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-776.
    Strain: ATCC 204508 / S288c.
  6. "The PY-motif of Bul1 protein is essential for growth of Saccharomyces cerevisiae under various stress conditions."
    Yashiroda H., Kaida D., Toh-e A., Kikuchi Y.
    Gene 225:39-46(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN, INTERACTION WITH RSP5, MUTAGENESIS OF PRO-157 AND PRO-158.
  7. "Yeast glycogen synthase kinase 3 is involved in protein degradation in cooperation with Bul1, Bul2, and Rsp5."
    Andoh T., Hirata Y., Kikuchi A.
    Mol. Cell. Biol. 20:6712-6720(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Ubiquitin is required for sorting to the vacuole of the yeast general amino acid permease, Gap1."
    Soetens O., De Craene J.-O., Andre B.
    J. Biol. Chem. 276:43949-43957(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease."
    Helliwell S.B., Losko S., Kaiser C.A.
    J. Cell Biol. 153:649-662(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Rsp5-Bul1/2 complex is necessary for the HSE-mediated gene expression in budding yeast."
    Kaida D., Toh-e A., Kikuchi Y.
    Biochem. Biophys. Res. Commun. 306:1037-1041(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX, MUTAGENESIS OF PRO-157 AND PRO-158.
  11. "Pressure-induced differential regulation of the two tryptophan permeases Tat1 and Tat2 by ubiquitin ligase Rsp5 and its binding proteins, Bul1 and Bul2."
    Abe F., Iida H.
    Mol. Cell. Biol. 23:7566-7584(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX.
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Genetic, biochemical, and transcriptional responses of Saccharomyces cerevisiae to the novel immunomodulator FTY720 largely mimic those of the natural sphingolipid phytosphingosine."
    Welsch C.A., Roth L.W.A., Goetschy J.F., Movva N.R.
    J. Biol. Chem. 279:36720-36731(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "NPR1 kinase and RSP5-BUL1/2 ubiquitin ligase control GLN3-dependent transcription in Saccharomyces cerevisiae."
    Crespo J.L., Helliwell S.B., Wiederkehr C., Demougin P., Fowler B., Primig M., Hall M.N.
    J. Biol. Chem. 279:37512-37517(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX.
  16. "Ubiquitin-mediated targeting of a mutant plasma membrane ATPase, Pma1-7, to the endosomal/vacuolar system in yeast."
    Pizzirusso M., Chang A.
    Mol. Biol. Cell 15:2401-2409(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX.
  17. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  18. "Transduction of the nitrogen signal activating Gln3-mediated transcription is independent of Npr1 kinase and Rsp5-Bul1/2 ubiquitin ligase in Saccharomyces cerevisiae."
    Feller A., Boeckstaens M., Marini A.-M., Dubois E.
    J. Biol. Chem. 281:28546-28554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX.
  19. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  20. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  21. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBUL1_YEAST
AccessioniPrimary (citable) accession number: P48524
Secondary accession number(s): D6W0A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 486 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3