P48516 (IF3_MYXXA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-3

Gene names

Name:	infC
Synonyms:	dsg

Organism

Myxococcus xanthus

Taxonomic identifier

34 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. HAMAP MF_00080
Subunit structure	Monomer By similarity. HAMAP MF_00080
Subcellular location	Cytoplasm By similarity HAMAP MF_00080.
Domain	The 66 residues C-terminal tail is not present in other species. HAMAP MF_00080
Sequence similarities	Belongs to the IF-3 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 247	246	Translation initiation factor IF-3 HAMAP MF_00080		PRO_0000177547
Regions
	Region	182 – 247	66	Needed for vegetative and developmental functions, but not for viability HAMAP MF_00080
Sites
	Site	109	1	Important for 30S binding By similarity
	Site	112	1	Important for 30S binding By similarity
Natural variations
	Natural variant	134	1	G → E in strain: DK429 and DK439; causes defects in fruiting body development and in sporulation.

Sequences

	Sequence		Length	Mass (Da)
	P48516 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6052DAC6407FAEA7	FASTA	247	27,145
	10 20 30 40 50 60 MIREQRSSRG GSRDQRTNRR IRAREVRVVG SDGSQLGVMP LEAALDRART EGLDLVEISP 70 80 90 100 110 120 MASPPVCKIM DYGKFKYEEK KKASEAKRAQ VTVLLKEVKL RPKTEEHDYE FKVRNTRRFI 130 140 150 160 170 180 EDGNKAKVVI QFRGREITHR EQGTAILDDV AKDLKDVAVV EQMPRMEGRL MFMILAPTPK 190 200 210 220 230 240 VAQKARELVR QAATAAKRPP PPGAPGAGKS AAGASSGAEE KAEETAEEKK EAQAAPAAAE AQSPTAS

References

[1]	"The dsg gene of Myxococcus xanthus encodes a protein similar to translation initiation factor IF3." Cheng Y.L., Kalman L.V., Kaiser D. J. Bacteriol. 176:1427-1433(1994) [PubMed: 8113184] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-7. Strain: DK101.
[2]	"The Myxococcus xanthus dsg gene product performs functions of translation initiation factor IF3 in vivo." Kalman L.V., Cheng Y.L., Kaiser D. J. Bacteriol. 176:1434-1442(1994) [PubMed: 8113185] [Abstract] Cited for: CHARACTERIZATION.
[3]	"dsg, a gene required for cell-cell interaction early in Myxococcus development." Cheng Y.L., Kaiser D. J. Bacteriol. 171:3719-3726(1989) [PubMed: 2544552] [Abstract] Cited for: CHARACTERIZATION. Strain: DK101.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U04438 Unassigned DNA. Translation: AAC13748.1.
PIR	A53379.
3D structure databases
ProteinModelPortal	P48516.
SMR	P48516. Positions 14-88.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00080. IF-3. [Tree]
InterPro	IPR019813. Translation_initiation_fac3_CS. IPR001288. Translation_initiation_fac_3. IPR019815. Translation_initiation_fac_3_C. IPR019814. Translation_initiation_fac_3_N. [Graphical view]
Gene3D	G3DSA:3.10.20.80. IF3. 1 hit. G3DSA:3.30.110.10. IF3. 1 hit.
PANTHER	PTHR10938. IF3. 1 hit.
Pfam	PF00707. IF3_C. 1 hit. PF05198. IF3_N. 1 hit. [Graphical view]
SUPFAM	SSF54364. IF3. 1 hit. SSF55200. IF3. 1 hit.
TIGRFAMs	TIGR00168. InfC. 1 hit.
PROSITE	PS00938. IF3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF3_MYXXA

Accession

Primary (citable) accession number: P48516

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	May 31, 2011
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program